期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1236, 期 -, 页码 115-122出版社
ELSEVIER
DOI: 10.1016/j.chroma.2012.03.010
关键词
Peptides; Ion exchange; Centrifugal partition chromatography; DOSY NMR
资金
- University of Reims Champagne-Ardenne
- CNRS
- Ministere de l'Enseignement Superieur et de la Recherche
This article presents the scope and optimization strategies employed in ion-exchange centrifugal partition chromatography (IXCPC). Both the weak and the strong modes were used to separate the constituents of a model mixture of dipeptides. Thus, the combined use of the quaternary biphasic solvent system, methyl-tert-butylether/acetonitrile/n-butanol/water (2:1:2:5, v/v) in the descending mode, of the lipophilic di(2-ethylhexyl)phosphoric acid (DEHPA) cation-exchanger, and of two displacers: calcium chloride and hydrochloric acid, has proven to be efficient for the preparative separation of the model mixture of five di peptides (GG, GY, AY, LV and LY, in the order they were collected). The separation was optimized by splitting the stationary phase into two sections that differed by their triethylamine concentration. Moreover, the chemical nature of the exchanger/analyte entities that were involved in the chromatographic process was determined by P-31 and H-1 DOSY NMR experiments. (C) 2012 Elsevier B.V. All rights reserved.
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