Article
Biochemical Research Methods
Ming Huang, Xiaobin Xu, Haibo Qiu, Ning Li
Summary: In this study, a generic HILIC-MS/MS method was developed for in-depth characterization of nucleic acid therapeutic modalities. High-resolution mass spectrometry was used to determine the deconvoluted masses of oligonucleotide and siRNA standards and their impurities. Optimized higher-energy C-trap dissociation was used to improve the sequence coverage of DNA and RNA oligonucleotides, achieving higher on-column sensitivity for phosphorothioate oligonucleotide analysis.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
M. Mar Aparicio-Muriana, Rocio Carmona-Molero, Francisco J. Lara, Ana M. Garcia-Campana, Monsalud del Olmo-Iruela
Summary: The development of a HILIC-MS/MS multiclass method for simultaneous analysis of eight cyanotoxins in reservoir water samples is described in this work, providing a reliable and sensitive analytical approach for cyanotoxin analysis. The study shows that a tandem solid-phase extraction system can effectively extract and preconcentrate different classes of cyanotoxins.
Article
Chemistry, Analytical
Dun-Jian Wang, Da-Wei Wang, Qiu-Chen Fang, Ye Shen, Nv-Jin Zeng, Yan-Ling Yang, Hong-Wen Zhang, Yong-Qing Wang, Lu-Ning Sun
Summary: A simple, fast, and sensitive tandem mass spectrometry method was established to detect the concentration of regadenoson in human plasma, which was used to study the pharmacokinetic characteristics of regadenoson in healthy Chinese subjects. The method showed linear calibration curve, low precision, high accuracy, and no matrix effect for regadenoson or regadenoson-d3.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Applied
Xixi Jia, Xiaowei Li, Liang Zhao, Saiwa Liu, Zhinan Wang, Jingjing Du, Lirui Ge, Kunxia Zhao, Lu Tian, Jian Xu, Zuhao Yan, Jiangyi Ding, Xi Xia
Summary: A sensitive and selective method using hydrophilic interaction chromatography combined with tandem mass spectrometry was developed to detect 18 aminoglycosides in bovine milk. Aminoglycosides were extracted and isolated from milk using a mixture of trichloroacetic acid, ammonium acetate, ethylenediaminetetraacetic acid, and sodium chloride, and an Oasis HLB solid-phase extraction cartridge. The method showed satisfactory separation of the analytes with detection and quantification limits ranging from 0.05-2 μg/kg and 0.1-5 μg/kg, respectively. The method was successfully applied to detect neomycin in one fresh bovine milk sample, and it provides a powerful tool for the surveillance of aminoglycosides in bovine milk.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Food Science & Technology
Zhixing Gu, Xi Nie, Ping Guo, Yuehua Lu, Bo Chen
Summary: An analytical method using HPLC with diode array and evaporative light scattering detectors was established for the simultaneous determination of hydrophobic and hydrophilic compounds in the dried rhizome of Atractylodes macrocephala Koidz. The method provides a new choice for the quality control of traditional Chinese medicines and health foods.
Article
Biochemical Research Methods
Elif Ozturk Er, Belma Ozbek, Sezgin Bakirdere
Summary: A novel analytical method was described for the determination of underivatized amino acids in human plasma and urine samples, achieving accurate results through liquid chromatography-tandem mass spectrometry, demonstrating high reliability and reproducibility.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Bingchuan Wei, Jenny Wang, Lulu Dai, Bifan Chen, Kelly Zhang
Summary: This study developed a method for analyzing sgRNA and demonstrated the potential of HILIC-MS for analyzing large oligonucleotides.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Marta Passamonti, Chiem de Roos, Peter J. Schoenmakers, Andrea F. G. Gargano
Summary: In this study, polymerization mixture was optimized to synthesize poly(acrylamide-co-N,N'-methylenebisacrylamide) monolithic stationary phases for HILIC of intact proteins. The monolithic structure exhibited a permeable and polar stationary phase suitable for HILIC. The monolith showed superior separation performance and lower ion interaction compared to silica-based particulate amide phase, enabling successful hyphenation with high-resolution mass spectrometry in HILIC of intact proteins.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Michaela Chocholouskova, Denise Wolrab, Robert Jirasko, Hana Studentova, Bohuslav Melichar, Michal Holcapek
Summary: This study systematically investigates the effects of different lipid class separation methods and mass spectrometers on quantitative lipidomic data, finding slight variations in molar concentrations even within the same laboratory using different platforms. Normalization to a reference sample can significantly reduce these differences, leading to highly homogenous molar concentrations of individual lipid species. This strategy suggests a potential approach for reporting comparable quantitative results regardless of the analytical platform and mass spectrometer used.
Article
Chemistry, Analytical
Akinori Yamaguchi, Hajime Miyaguchi, Manabu Tokeshi
Summary: This study developed a screening method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to detect and identify Novichok A-series degradation products and alkyl methylphosphonic acids in urine samples. The method utilized a suitable derivatization reagent and achieved the detection and stability of the target compounds. The newly developed method was successfully applied to analyze urine samples from a proficiency test.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Quanshou Feng, Zehu Xie, Hongze Liang, Chuan-Fan Ding, Yinghua Yan
Summary: Ovalbumin is a significant protein source, but the safety concerns of protein allergy and contamination have increased. Hence, it is necessary to detect proteins efficiently and rapidly. Mass spectrometry is a powerful tool used in protein detection. In this study, COF@S-S@GC was prepared and showed sensitive detection, good selectivity, high adsorption capacity, stability, reproducibility, linearity, LOQ level, and recovery ratio for ovalbumin. Furthermore, COF@S-S@GC also demonstrated satisfactory purification ability for protein enrichment from complex samples, indicating its great potential in protein analysis.
Article
Chemistry, Analytical
Szabolcs Fekete, Mathew DeLano, Andrew Bates Harrison, Stephen J. Shiner, Jonathan Leon Belanger, Kevin D. Wyndham, Matthew A. Lauber
Summary: In order to address the challenges in analyzing certain biomolecules using liquid chromatography, researchers have developed a new hydrophilically modified hybrid surface (h-HST) for size exclusion chromatography and anion exchange separations. By incorporating additional oxygen and carbon atoms onto an ethylene bridge hybrid siloxane polymer, this surface reduces non-specific adsorption and undesired secondary interactions, resulting in improved separations of proteins and nucleic acids.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Majidah Alsaeedi, Huda Alghamdi, Phyllis Hayes, Anna M. Hogan, Elizabeth S. Gilchrist, Kirsten G. Dowling, Jane A. English, Jeremy D. Glennon
Summary: Nickel (Ni) is a trace heavy metal with documented allergy and carcinogenic effects in humans. Understanding the coordination mechanisms and labile complex species of Ni(II) is crucial for understanding its biological effects. Histidine (His) is an essential amino acid that contributes to protein structure and coordination of Ni(II) ions. This study evaluated different chromatographic columns for the separation of Ni(II)-Histidine species, and the Zic-cHILIC column showed high efficiency and selectivity. The identities of the Ni(II)-Histidine species were confirmed using HILIC-ESI-MS.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Olatomiwa O. Bifarin, David A. Gaul, Samyukta Sah, Rebecca S. Arnold, Kenneth Ogan, Viraj A. Master, David L. Roberts, Sharon H. Bergquist, John A. Petros, Facundo M. Fernandez, Arthur S. Edison
Summary: This study utilized liquid chromatography-mass spectrometry and nuclear magnetic resonance data, combined with machine learning methods, to discover candidate metabolomic panels for renal cell carcinoma.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemical Research Methods
Kristian Serafimov, Michael Laemmerhofer
Summary: In this study, a targeted approach with wide metabolite coverage was developed for cellular metabolomic analysis using a UHPLC-QTrap-MS system operated in the scheduled multiple reaction monitoring (sMRM) mode. The study compared four different cell extraction protocols and developed two HILIC-MS methods. A total of 161 metabolites were successfully detected in ESI+ mode, and 92 were detected in negative ionization mode. The developed methods were calibrated and validated, showing good accuracy and precision.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Agriculture, Multidisciplinary
Thierry Sayd, Christophe Chambon, Milka Popova, Diego P. Morgavi, Angelique Torrent, Sylvie Blinet, Laetitia Theron, Vincent Niderkorn
Summary: The study aimed to investigate the effects of tannins on plant protein digestion in sheep by combining in vivo and in vitro approaches. The results showed that tannins reduced the production of protein degradation products and increased the abundance of RuBisCo protein. This protective effect was observed during early-stage intestinal digestion but not in the late-stage.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Review
Nutrition & Dietetics
Francesco Maria Calabrese, Annalisa Porrelli, Mirco Vacca, Blandine Comte, Katharina Nimptsch, Mariona Pinart, Tobias Pischon, Estelle Pujos-Guillot, Maria De Angelis
Summary: Low-grade inflammatory diseases have been found to be associated with metabolic disturbances, such as gut microbiota dysbiosis. Metaproteomics has been used in the past decade to investigate protein composition in specific healthy and pathological conditions. This review applied a rigorous protocol to select 10 metaproteomics studies aiming at investigating obesity and diabetes, providing insights into microbial and human metaproteome alterations and metabolic patterns in individuals affected by diabetes and obesity. However, the lack of metaproteomic data limits objective considerations, and the establishment of specific databases and standardized analysis protocols is needed to further enhance our knowledge on these prevalent diseases.
Article
Nutrition & Dietetics
Valeria Agamennone, Peter M. Abuja, Marijana Basic, Maria De Angelis, Andre Gessner, Bart Keijser, Martin Larsen, Mariona Pinart, Katharina Nimptsch, Estelle Pujos-Guillot, Kristina Schlicht, Itai Sharon, Eva Untersmayr, Matthias Laudes, Tobias Pischon, Jildau Bouwman
Summary: Studies show that the intestinal microbiota has an influence on general metabolic processes and the risk of chronic diseases in humans. Dietary factors also play a role and affect the composition and function of the gut microbiota. The HDHL-INTIMIC knowledge platform aims to foster research on the microbiota, nutrition, and health by assembling available knowledge and sharing information with the scientific community.
Review
Food Science & Technology
Dragan Milenkovic, Frederic Capel, Lydie Combaret, Blandine Comte, Dominique Dardevet, Bertrand Evrard, Christelle Guillet, Laurent-Emmanuel Monfoulet, Alexandre Pinel, Sergio Polakof, Estelle Pujos-Guillot, Didier Remond, Yohann Wittrant, Isabelle Savary-Auzeloux
Summary: Impairment of gut function is a mechanism for the decline in health status in the elderly, involving declines in digestive physiology, metabolism, and immune status. This is associated with changes in the composition and function of the gut microbiota. These alterations can be targeted through nutritional strategies to prevent or delay the occurrence of age-related pathologies.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Agriculture, Dairy & Animal Science
R. Huang, P. Romero, A. Belanche, E. M. Ungerfeld, D. Yanez-Ruiz, D. P. Morgavi, M. Popova
Summary: Some antimethanogenic feed additives for ruminants can lead to the accumulation of rumen dihydrogen (H2), which may affect optimal fermentation. However, combining an H2 acceptor with a methanogenesis inhibitor can decrease rumen H2 build-up and improve beneficial metabolite production for the host ruminant.
Article
Agriculture, Dairy & Animal Science
P. Romero, R. Huang, E. Jimenez, J. M. Palma-Hidalgo, E. M. Ungerfeld, M. Popova, D. P. Morgavi, A. Belanche, D. R. Yanez-Ruiz
Summary: Most mitigation strategies to reduce enteric methane production in the rumen result in an excess of rumen dihydrogen that is not utilized efficiently. This study investigated the potential of phenolic compounds to act as hydrogen acceptors, thereby improving rumen fermentation and reducing methane production. The results showed that Asparagopsis taxiformis effectively inhibited methane production, increased hydrogen accumulation, and improved rumen fermentation in goats.
Review
Agriculture, Dairy & Animal Science
D. P. Morgavi, G. Cantalapiedra-Hijar, M. Eugene, C. Martin, P. Noziere, M. Popova, I. Ortigues-Marty, R. Munoz-Tamayo, E. M. Ungerfeld
Summary: The inhibition of methane emissions in livestock does not result in consistent improvements in production, and the expected improvement in net energy for production is small and difficult to detect. Therefore, the claim that enteric methane inhibition will lead to more feed-efficient animals is not supported.
Article
Ecology
Thea O. Andersen, Ianina Altshuler, Arturo Vera-Ponce de Leon, Juline M. Walter, Emily McGovern, Kate Keogh, Cecile Martin, Laurence Bernard, Diego P. Morgavi, Tansol Park, Zongjun Li, Yu Jiang, Jeffrey L. Firkins, Zhongtang Yu, Torgeir R. Hvidsten, Sinead M. Waters, Milka Popova, Magnus o. Arntzen, Live H. Hagen, Phillip B. Pope
Summary: Protozoa, including entodiniomorphs and holotrichs, play a major role in the microbial biomass of the rumen microbiome. However, their specific biological and metabolic contributions to rumen function have not been well described. By using metaproteomics, this study reveals the specific metabolic niches occupied by protozoa in relation to their microbial co-habitants in the rumen fluid of cattle and goats fed varying diets.
Editorial Material
Microbiology
Robert W. Li, Christina D. Moon, Diego P. Morgavi
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Marion Brandolini-Bunlon, Benoit Jaillais, Veronique Cariou, Blandine Comte, Estelle Pujos-Guillot, Evelyne Vigneau
Summary: In nutrition and health research, untargeted metabolomics is analyzed together with clinical data to improve prediction and understanding of pathological status. This can be modeled using a multiblock supervised model, where different input data blocks are potential predictors of the outcome. Path diagrams can also be used to represent this configuration, allowing for accounting of block effects.
Article
Microbiology
Mahendra Mariadassou, Laurent X. X. Nouvel, Fabienne Constant, Diego P. P. Morgavi, Lucie Rault, Sarah Barbey, Emmanuelle Helloin, Olivier Rue, Sophie Schbath, Frederic Launay, Olivier Sandra, Rachel Lefebvre, Yves Le Loir, Pierre Germon, Christine Citti, Sergine Even
Summary: In this study, dairy cows with different susceptibility to mastitis were examined to explore the relationships between microbiota composition and various factors in different body sites. The results showed that each body site harbored a specific microbial community that changed over time. There was a significant sharing of microbes between anatomical sites within each animal, but limited sharing between animals. The host genetics were found to be related to the microbial composition in milk.
Article
Multidisciplinary Sciences
Gonzalo Cantalapiedra-Hijar, Gonzalo Martinez-Fernandez, Evelyne Forano, Stuart E. Denman, Diego Morgavi, Christopher S. Mcsweeney
Summary: This study investigates the impact of rumen bacteria on nitrogen isotopic discrimination and confirms their key role in Δ15Nanimal-diet.
Meeting Abstract
Gastroenterology & Hepatology
Benjamin Buchard, Natali Abeywickrama Samarakoon, Estelle Pujos-Guillot, Stephanie Durand, Melanie Petera, Delphine Centeno, Juliette Joubert, Aicha Demidem, Armand Abergel
JOURNAL OF HEPATOLOGY
(2022)
Article
Multidisciplinary Sciences
Rafael Munoz-Tamayo, Birte L. Nielsen, Mohammed Gagaoua, Florence Gondret, E. Tobias Krause, Diego P. Morgavi, I. Anna S. Olsson, Matti Pastell, Masoomeh Taghipoor, Luis Tedeschi, Isabelle Veissier, Christian Nawroth
Summary: This article provides practical steps to embrace Open Science in animal science, aiming to promote the field's development, increase public trust, and address future challenges.
Article
Endocrinology & Metabolism
Nils Paulhe, Cecile Canlet, Annelaure Damont, Lindsay Peyriga, Stephanie Durand, Catherine Deborde, Sandra Alves, Stephane Bernillon, Thierry Berton, Raphael Bir, Alyssa Bouville, Edern Cahoreau, Delphine Centeno, Robin Costantino, Laurent Debrauwer, Alexis Delabriere, Christophe Duperier, Sylvain Emery, Amelie Flandin, Ulli Hohenester, Daniel Jacob, Charlotte Joly, Cyril Jousse, Marie Lagree, Nadia Lamari, Marie Lefebvre, Claire Lopez-Piffet, Bernard Lyan, Mickael Maucourt, Carole Migne, Marie-Francoise Olivier, Estelle Rathahao-Paris, Pierre Petriacq, Julie Pinelli, Lea Roch, Pierrick Roger, Simon Roques, Jean-Claude Tabet, Marie Tremblay-Franco, Mounir Traikia, Anna Warnet, Vanessa Zhendre, Dominique Rolin, Fabien Jourdan, Etienne Thevenot, Annick Moing, Emilien Jamin, Francois Fenaille, Christophe Junot, Estelle Pujos-Guillot, Franck Giacomoni
Summary: A comprehensive resource, PeakForest addresses the technical bottleneck in annotating large-scale spectral data and identifying metabolites in metabolomics laboratories. It provides a structured database with tools for data curation and browsing, facilitating the sharing and integration of spectral data across laboratories.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)