Article
Agricultural Engineering
Fernando Roberto Paz-Cedeno, Jose Miguel Carceller, Sara Iborra, Ricardo Keitel Donato, Anselmo Fortunato Ruiz Rodriguez, Marco Antonio Morales, Eddyn Gabriel Solorzano-Chavez, Ismael Ulises Miranda Roldan, Ariela Veloso de Paula, Fernando Masarin
Summary: Immobilized enzymes on magnetic graphene oxide particles improve stability and reusability, showing potential applications in enzymatic hydrolysis of sugarcane bagasse.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Energy & Fuels
Prabhpreet Kaur, Monica Sachdeva Taggar, Anu Kalia
Summary: In this study, cellulase produced from Aspergillus fumigatus was immobilized on magnetic nanoparticles using glutaraldehyde as a cross linker, exhibiting good binding efficiency and activity. The immobilized cellulase showed high efficiency and reusability in hydrolyzing rice straw, indicating promising potential for industrial production of fermentable sugars and ethanol.
BIOMASS CONVERSION AND BIOREFINERY
(2021)
Article
Biochemistry & Molecular Biology
Dianyu Yu, Ziyue Li, Xiaonan Zhou, Weining Wang, Liqi Wang, Tianyi Liu, Jing Du
Summary: In this study, MNPs were combined with GO to improve the immobilization effect of lipase. The modified MGON with TMSPU showed potential for enzyme immobilization applications, displaying good thermal stability and high enzyme activity retention after multiple reuse.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Inorganic & Nuclear
Jaya Sharma, Parveen Kumar, Mika Sillanpaa, Dinesh Kumar, Meena Nemiwal
Summary: Ionic liquids are considered as eco-sustainable solvents and have great potential in chemical reactions. Magnetic nanoparticle-supported ILs have attracted much attention due to their outstanding qualities. However, coating of nanoparticles with silica layer is required to prevent aggregation and deactivation.
INORGANIC CHEMISTRY COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Guozhen Chen, Jianbin Zheng
Summary: In this study, a novel electrochemical nitrite sensor was developed using a GO-PANI-AuNPs nanocomposite, which exhibited excellent catalytic performance and selectivity for nitrite oxidation. The sensor was successfully applied to the detection of nitrite ions in tap water and sewage, offering a reliable electrochemical strategy for environmental monitoring applications.
MICROCHEMICAL JOURNAL
(2021)
Article
Biophysics
Hengyu Liu, Changchun Hao, Yanyan Zhang, Haiyan Yang, Runguang Sun
Summary: In this study, graphene oxide-silver nanoparticles (GO-AgNPs) hybrids were synthesized using a one-pot method. Various methods were employed to characterize the interaction between GO-AgNPs and trypsin, revealing that GO-AgNPs can enhance trypsin activity and promote the hydrolysis of BSA. This provides important support for the application of GO-based nanocomposites in enzyme immobilization.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Chemistry, Applied
Bin Gu, Qimeng Jiang, Bichong Luo, Chuanfu Liu, Junli Ren, Xiaohui Wang, Xiaoying Wang
Summary: A new nanocomposite film was synthesized in this study, showing durable antibacterial effect against E. coli and S. aureus and no toxicity to cells.
CARBOHYDRATE POLYMERS
(2021)
Article
Materials Science, Multidisciplinary
Gabriel Cardoso Pinto, Guilherme Nunes Lucena, Rodolfo Debone Piazza, Joao Miguel Lopes Costa, Eduardo Torres Couto Coimbra Silva, Yuanyu Gu, Ariela Veloso de Paula, Nuno Joao Oliveira Silva, Rodrigo Fernando Costa Marques
Summary: This study successfully immobilized cellulases and lipases enzymes using reusable enzyme carriers and evaluated their enzymatic activity under normal and alternating magnetic field conditions. It was found that increasing the reaction frequency can reduce the decrease in enzyme activity at different pH values. Additionally, the magnetic properties of the carrier and reaction medium facilitated successful recycling experiments. In conclusion, immobilizing cellulase and lipases enzymes into MGO derivatives shows great potential and suitability for the conversion of cellulose and long-chain acyl esters into valuable compounds.
MATERIALS TODAY COMMUNICATIONS
(2023)
Article
Engineering, Environmental
Weiguo Huang, Yunru Yu, Chaoyu Yang, Xiaohui Zhang, Luoran Shang, Yan Zu, Keqing Shi
Summary: A novel magnetic graphene oxide nanoparticles (MGONs) have been developed for efficient capture of exosomes. The capture process does not affect the structure and function of the exosomes. The concentration of captured exosomes can be measured by combining with a fluorescence probe. These MGONs have significant potential in early disease diagnosis and clinical applications.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Energy & Fuels
Murilo Barbosa Andrade, Tassia Rhuna Tonial Santos, Ana Carolina Sestito Guerra, Marcela Fernandes Silva, Gabriela Maria Matos Demiti, Rosangela Bergamasco
Summary: This study developed a new adsorbent material GO#α-γ-Fe2O3Co3O4, which showed high efficiency and excellent regenerative capacity in caffeine adsorption experiments. Thermodynamic parameters indicated that caffeine adsorption process is exothermic, spontaneous, and favorable. Ion competition experiments found that Na+, K+, Ca2+, and Mg2+ ions do not interfere with the adsorption process.
CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION
(2022)
Article
Engineering, Chemical
Yangchao Liu, Senqing Fan, Yu Chen, Jiaojiao Chen, Jiaxin Meng, Mingxia Yang, Chuang Li, Haijie Qing, Zeyi Xiao
Summary: A catalytic membrane nano reactor (CMNR) with two-dimensional channels and Co3S4 catalyst immobilized on graphene oxide (GO) nanosheets has been successfully fabricated. The CMNR shows excellent catalytic performance with high conversion and stability, even in the presence of humic acid.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Daniela F. Baez
Summary: Graphene-based nanomaterials, specifically graphene oxide (GO) and reduced graphene oxide (rGO), have shown great potential in cancer therapy. This review explores the structural features of GO and rGO, and discusses the applicability of GBNMs in photothermal therapy for different cancer types. It also examines the integration of photothermal therapy with other treatment strategies and the use of hybrid magnetic-GBNMs. The challenges and prospects of clinical translation are also addressed.
Article
Chemistry, Multidisciplinary
Baishali Mahanta, Hasan Al Mamun, Rana Sanjay Kumar Singh, Lakhya Jyoti Borthakur
Summary: A MoS2/SG/Cu nanocomposite was prepared and used as a sensor electrode for the simultaneous electrochemical sensing of DA and 5-HT in urine samples with high stability and sensitivity.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Analytical
Vedran Milosavljevic, Katerina Mitrevska, Vojtech Adam
Summary: Graphene nanoparticles (GNPs) show promise in biosensing applications due to their high electrochemical activity and optical properties. Oxygenated counterparts, GONPs, offer improved dispersibility and stability.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemical Research Methods
Lekshmi M. S. Sundar, K. Madhavan Nampoothiri
Summary: In this study, xylose dehydrogenase was immobilized on ferromagnetic nanoparticles to efficiently convert xylose into xyloonic acid. The optimized parameters resulted in a maximum conversion rate of 91%, and the immobilized enzyme showed a 2-fold increase in reaction velocity and catalytic efficiency compared to the free enzyme. The enzyme-nanoparticle conjugate retained 93% relative activity after 10 successive experiments, demonstrating its good recyclability for XA production.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Gastroenterology & Hepatology
Jonathan Yde, Qi Wu, Johan F. Borg, Robert A. Fenton, Hanne B. Moeller
Summary: Bile acid diarrhea is a chronic condition caused by increased delivery of bile acids to the colon. This study used a rat model to investigate genes associated with bile acid diarrhea, and identified genes involved in electrolyte and water transport as well as bile acid transport. The study also provided annotated rat colonic epithelial cell transcriptome and proteome with response to bile acid feeding.
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2022)
Article
Chemistry, Analytical
Hongwei Chu, Qun Zhao, Ju Liu, Kaiguang Yang, Yanlei Wang, Jianhui Liu, Kun Zhang, Baofeng Zhao, Hongyan He, Yong Zheng, Shijun Zhong, Zhen Liang, Lihua Zhang, Yukui Zhang
Summary: Efficient extraction of hydrophobic membrane protein complexes (MPCs) has always been a challenge due to stability concerns, but the novel i-TAN extraction system utilizing TEAA IL and NP-40 shows superior performance in stabilizing PPIs and extracting MPCs. This system allows for confident identification of protein interactions and provides insights into drug resistance mechanisms. The study presents a novel strategy for MPC extraction and downstream processing, highlighting the potential of i-TAN in interactomics and functional analysis of MPCs.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yechen Hu, Bo Jiang, Jianhui Liu, He Wang, Zhigang Sui, Baofeng Zhao, Zhen Liang, Lihua Zhang, Yukui Zhang
Summary: Protein N-phosphorylation is a critical post-translational modification in central metabolism and cell signaling. However, the other functions of N-phosphorylation in organisms are largely unexplored due to the lack of efficient enrichment methods. In this study, a graphene oxide nanocomposite with large immobilization amount and excellent solubility was designed and verified to have excellent enrichment ability for N-phosphoproteins. This method provides an alternative for the discovery of N-phosphoproteins with significant biological functions.
Article
Engineering, Biomedical
Baofu Ma, Kun Zhang, Zhen Sun, Hui Pan, Kaiguang Yang, Bo Jiang, Baofeng Zhao, Zhen Liang, Yukui Zhang, Lihua Zhang
Summary: A unique pushpin-like Au/CeO2 hybrid nanozyme with high catalytic activity was designed by combining site-selective growth and steric restriction strategies. The nanozyme exhibited superior antitumor effects both in vitro and in vivo due to its high catalytic activity and targeting effects. The reactive oxygen species (ROS) generated by the nanozyme caused damage in tumor cells and evoked a response to oxidative stress and innate immunity in vivo.
ACTA BIOMATERIALIA
(2023)
Article
Biochemical Research Methods
Hui Pan, Baofu Ma, He Wang, Xulian Cui, Kun Zhang, Baofeng Zhao, Bo Jiang, Zhen Liang, Lihua Zhang, Yukui Zhang
Summary: Besides O-phosphorylation, protein N-phosphorylation plays important roles in various biological processes. Enriching N-phosphorylation peptides in tissue is challenging due to its complexity. In this study, a workflow for identifying N-phosphorylation peptides in mouse brain tissue was established using direct enrichment in high concentration urea. The method was reliable and successfully applied to analyze N-phosphorylation in a mouse model of Alzheimer's disease, revealing potential links between N-phosphorylation and Alzheimer's disease-related processes.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Chemistry, Analytical
Wen Zhou, Zhigang Sui, Jianhui Liu, Yingyun He, Huiming Yuan, Yue Sun, Zhen Liang, Kaiguang Yang, Lihua Zhang, Yukui Zhang
Summary: In this study, a high-sensitivity and high-selectivity diagnostic method based on mass spectrometry was developed to specifically identify the SARS-CoV-2 S1 glycoprotein. This technology demonstrated a detection sensitivity of 0.01 pg even in the presence of other structural proteins. It not only validates the presence of the SARS-CoV-2 virus, but also holds potential for detecting other pathogens.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Xinwei Li, Yuwan Chen, Jing Jiang, Wen Zhou, Hang Gao, Qun Zhao, Zhen Liang, Xupeng Cao, Kaiguang Yang, Lihua Zhang, Yukui Zhang
Summary: This study developed a new method to achieve in vivo crosslinking and mapping of photosynthetic membrane protein complexes in living cells. By delivering BSPNO crosslinker into the chloroplasts using nanocarriers, the method overcame the limitations of previous techniques and captured weak interactions and uncharacterized protein functions in photosynthetic protein complexes.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Baofu Ma, Jianyu Han, Qun Zhao, Bo Jiang, Zhixin Zhang, Baofeng Zhao, Zhen Liang, Yukui Zhang, Lihua Zhang
Summary: Pt/CeO2-R has shown high enzyme-like activity, selective toxicity to tumor cells, induction of tumor cell apoptosis, and inhibition of cell migration. Mechanistically, Pt/CeO2-R affects mitochondria and endoplasmic reticulum by producing reactive oxygen species, leading to tumor cell apoptosis. Additionally, Pt/CeO2-R inhibits cell migration by downregulating proteins on the cell membrane surface.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Beirong Zhang, Zhou Gong, Lili Zhao, Yuxin An, Hang Gao, Jing Chen, Zhen Liang, Maili Liu, Yukui Zhang, Qun Zhao, Lihua Zhang
Summary: Protein dynamics are crucial for their diverse functions, and are significantly influenced by the intracellular environment. This study introduces a hierarchical decoding strategy that uses chemical cross-linking mass spectrometry to investigate protein dynamics in cells. Computational analysis based on distance restraints derived from cross-links is used to infer protein dynamics in vivo.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Ecology
Zenghu Zhang, Dehai Li, Ruize Xie, Ruoyu Guo, Shailesh Nair, Huan Han, Guojian Zhang, Qun Zhao, Lihua Zhang, Nianzhi Jiao, Yongyu Zhang
Summary: Algae and bacteria in the ocean have complex interactions, and bacteria have developed anti-algal strategies. This study reveals a novel strategy in which a marine bacterium produces 4-BP to inhibit phytoplankton by affecting key metabolic processes. The presence of genes responsible for 4-BP synthesis in diverse bacterial members in the global ocean suggests its widespread use in mediating bacteria-algae antagonistic relationships.
Article
Multidisciplinary Sciences
Yuwan Chen, Wen Zhou, Yufei Xia, Weijie Zhang, Qun Zhao, Xinwei Li, Hang Gao, Zhen Liang, Guanghui Ma, Kaiguang Yang, Lihua Zhang, Yukui Zhang
Summary: Current methods for intracellular protein analysis often require the separation of specific organelles or changes to the intracellular environment. We demonstrate a method for in situ cross-linking and analysis of mitochondrial proteins in living cells. By delivering protein cross-linkers into mitochondria using poly(lactic-co-glycolic acid) (PLGA) nanoparticles functionalized with dimethyldioctadecylammonium bromide (DDAB), we identify novel protein-protein interactions and validate the data with structural analysis of mitochondrial respiratory chain proteins.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Han Ruonan, Zhao Lili, An Yuxin, Liang Zhen, Zhao Qun, Zhang Lihua, Zhang Yukui
Summary: Protein complexes play crucial roles in biological activities, and the CXMS technique offers a sensitive and high-throughput way to analyze their structures and interactions. However, the quality of the cross-linked peptide spectra needs improvement to enhance the identification of cross-linked sites.
CHINESE JOURNAL OF CHROMATOGRAPHY
(2022)
Article
Chemistry, Multidisciplinary
He Wang, Zhiting Wang, Hang Gao, Jianhui Liu, Zichun Qiao, Baofeng Zhao, Zhen Liang, Bo Jiang, Lihua Zhang, Yukui Zhang
Summary: This paper presents a photo-oxidation driven proximity labeling strategy for profiling the mitochondrial proteome with high spatiotemporal resolution in living cells. By selectively labeling mitochondrial proteins and quantifying their dynamics, this strategy provides solid technical support for the study of subcellular biology.
Article
Urology & Nephrology
Aihua Wu, Martin J. Wolley, Qi Wu, Diane Cowley, Johan Palmfeldt, Paul A. Welling, Robert A. Fenton, Michael Stowasser
Summary: Acute sodium chloride loading and volume expansion can reduce renal tubular reabsorption of sodium chloride and water in patients with hypertension. This modulation may be associated with changes in the sodium-chloride cotransporter (NCC), aquaporin-2 (AQP2), and urinary extracellular vesicle (uEV) proteins.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)