Article
Chemistry, Physical
Samaneh Fathi Darani, Farzaneh Ghazizadeh Ahsaie, Gholamreza Pazuki, Shiva Abdolrahimi
Summary: This study investigated the potential use of copolymers in aqueous two-phase systems, and the effect of UCON copolymer ATPS on the partitioning of doxorubicin. The results showed that the molecular weight of dextran affects the partition coefficient, while pH has minimal impact, and increasing the weight fraction of UCON enhances the partitioning of doxorubicin.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Green & Sustainable Science & Technology
Dipak Kumar Kanaujiya, Kannan Pakshirajan
Summary: This study investigates the biodegradation of DMP and DEP using a TPPB system. The results demonstrate that Cellulosimicrobium funkei can efficiently degrade these endocrine disrupting chemicals, highlighting the potential of the TPPB system for detoxification in the environment.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Engineering, Chemical
Petr A. Korchak, Evgenia A. Safonova, Alexey Victorov
Summary: This study systematically investigates experimental data and predictive capabilities of a family of aqueous biphasic systems. Improvements in the model are suggested for better performance. Results show that different chemical compositions affect the partitioning coefficients of L-tryptophan, indicating high potential for the extraction of biocomponents.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2021)
Article
Multidisciplinary Sciences
Damien Degoulange, Raj Pandya, Michael Deschamps, Dhyllan A. Skiba, Betar M. Gallant, Sylvain Gigan, Hilton B. de Aguiar, Alexis Grimaud
Summary: High-resolution Raman imaging is used to investigate the interface of salt-salt aqueous biphasic systems (ABSs) at a molecular level, revealing a gradual change of environment instead of a sharp molecular interface. This knowledge is of great importance in understanding aqueous interfaces and has significant implications in various biological and technological settings, including novel battery technologies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Engineering, Environmental
Michael J. Sargent, Nigel K. H. Slater, John S. Dennis, Gary J. Smith, Paul S. Pearlman
Summary: This study investigated the separation of Hexamethylenediamine (HMD) from dilute aqueous solutions using phase separation techniques, with sodium hydroxide (NaOH) found to significantly promote the phase separation. It was also discovered that sodium sulfate and sodium carbonate can enhance the phase separation, but are unable to induce phase separation without the presence of sodium hydroxide.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Engineering, Chemical
Hao Li, Liangdong Zhao, Shuo Yin, Chuanyu Chen, Haixiong Liu, Jing Fang
Summary: This study highlights the importance of a salt-free extraction system, with the K4P2O7/isopropanol aqueous phase system identified as the optimal choice for biofuel recovery.
Article
Chemistry, Physical
Jaran Patchimpet, Kanokphorn Sangkharak, Apiluck Eiad-ua, Sappasith Klomklao
Summary: The study investigated the recovery and partitioning of lipase from Nile tilapia viscera using a T-ATPS system, achieving high yield and partial purification of lipase by optimizing different partitioning parameters and conditions. Ultrasound was found to enhance the activity of lipase, demonstrating its potential as a technology for lipase recovery.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Green & Sustainable Science & Technology
Qian Li, Keping Zhang, Liangcheng Yang, Lichao Lu, Xianwang Kong, Xiangyu Zhao, Jianmeng Chen, Dongzhi Chen
Summary: Two-phase partitioning biotechnology is an effective method for enhancing the purification of hydrophobic waste-gas. However, current non-aqueous phases have limitations such as difficulty in recovery and low affinity for hydrophobic pollutants. In this study, a nano silicon oil-based ferrofluid was synthesized and used as an innovative non-aqueous phase liquid for the removal of gaseous n-hexane, achieving high removal efficiency and easy recovery.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Engineering, Chemical
Yuqiu Chen, Xiaodong Liang, Georgios M. Kontogeorgis
Summary: An optimal design method combining artificial neural network algorithm and group contribution method is developed in this study to predict the phase equilibrium composition and partition of biomolecules in aqueous two-phase systems (ATPS). By solving an optimization-based mixed-integer non-linear programming problem, the optimal ATPS is identified. Results show that our tailored ATPS exhibits significantly higher partitioning performance than reported ATPS in both cases.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Chemistry, Physical
Xiang Yang, Alberto Scacchi, Hossein Vahid, Maria Sammalkorpi, Tapio Ala-Nissila
Summary: We use the SPB theory to study the interaction between two parallel polyelectrolytes in monovalent ionic solutions. The theory accurately captures the interactions beyond the PE radius and accounts for positional correlations between charged groups. The results suggest that the SPB theory is an efficient way to model interactions in chemically specific complex PE systems.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Chemistry, Analytical
Yang Cao, Jingxuan Tian, Haisong Lin, Qingchuan Li, Yang Xiao, Huanqing Cui, Ho Cheung Shum
Summary: Most fluorescence-based bioanalytical applications require washing to remove excess labels, which is labor-intensive and hinders portable applications in resource-limited areas. In this study, an aqueous two-phase system (ATPS) is used for partitioning-induced isolation of labeled analytes without extra processing steps. The ATPS-based isolation is driven by affinity differences between analytes and excess labels. Different ATPS compositions were optimized for various scales of bioanalytical applications. The results showed successful isolation and analysis of analytes in centimeter-scale, millimeter-scale, and microscale ATPS.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Pranjala Tiwari, Blazej Podlesny, Maciej Krzywiecki, Karolina Z. Milowska, Dawid Janas
Summary: In this study, a Pluronic/Dextran system was developed to investigate the mechanism of the aqueous two-phase extraction (ATPE) technique for sorting SWCNTs and other nanomaterials. The role of phase-forming components and partitioning modulators was comprehensively studied to gain insights into the differentiation process. The results revealed the effectiveness of sodium dodecyl sulfate and sodium dodecylbenzene sulfonate as partitioning modulators for diameter-based sorting of SWCNTs. Additionally, the study suggested that the density differences of SWCNT species drive their movement in the ATPE system, resulting in a specific order of migration.
NANOSCALE HORIZONS
(2023)
Article
Chemistry, Physical
Diana C. Belchior, Mara G. Freire
Summary: Egg white proteins, such as ovalbumin and lysozyme, were effectively separated using aqueous biphasic systems (ABS) and three phase partitioning (TPP) methods. By optimizing the ABS conditions, selective precipitation of lysozyme over ovalbumin was achieved, demonstrating the potential of ABS/TTP combined strategies for fractionating high-value proteins from complex matrices in a single-step.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Biochemistry & Molecular Biology
Phei Er Kee, John Chi-Wei Lan, Hip Seng Yim, Yin Hui Chow, Po-Ting Chen, Hui-Suan Ng
Summary: Keratinase is efficiently recovered using aqueous two-phase electrophoresis system, achieving high partition coefficient, recovery yield, and purification fold under optimal conditions. The introduction of an electric field in the system significantly enhances the selectivity towards extracellular keratinase, demonstrating the efficiency of ATPES in separating keratinase from crude feedstock.
PROCESS BIOCHEMISTRY
(2021)
Article
Food Science & Technology
Xiao-Hua Zhang, Hui-Na Cui, Jing-Jing Zheng, Xiang-Dong Qing, Kai-Long Yang, Ya-Qian Zhang, Lu-Meng Ren, Le-Yuan Pan, Xiao-Li Yin
Summary: The flavor and aroma quality of green tea can be determined by the harvesting season using ATPS combined with chemometric analysis. The optimal ATPS was selected through single factor experiments and response surface methodology. The qualitative and quantitative analysis of green tea samples was carried out using HPLC-DAD and ATLD-MCR algorithm. Chemometric pattern recognition analysis showed that the PLS-DA and OPLS-DA models provided better results than the PCA model. The combination of ATPS and chemometric methods is accurate and reliable for the quality control of green tea.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Environmental Sciences
Caroline Maria Bezerra de Araujo, Gessica Wernke, Marcos Gomes Ghislandi, Alexandre Diorio, Marcelo Fernandes Vieira, Rosangela Bergamasco, Mauricio Alves da Motta Sobrinho, Alirio Egidio Rodrigues
Summary: This study demonstrates that the agar-graphene oxide (A-GO) hydrogel is a viable and eco-friendly alternative for the selective removal of chloroquine diphosphate and the cationic dye Safranin-O from water.
ENVIRONMENTAL RESEARCH
(2023)
Article
Thermodynamics
Alirio E. Rodrigues
Summary: In this article, the author reviews their work in the field of adsorption, summarizing their findings and suggesting a roadmap for future development. The author emphasizes the importance of understanding batch and continuous adsorption processes, as well as the prediction of adsorption equilibrium and the screening of adsorbents. They also discuss the challenges and opportunities associated with difficult separations and material choices, and highlight the significance of hybrid and data-driven modeling.
FLUID PHASE EQUILIBRIA
(2023)
Article
Chemistry, Physical
William M. Godoy, Leandro G. Aguiar, Nuno A. B. S. Graca, Alirio E. Rodrigues
Summary: This study synthesized sulfonated polymer resins based on styrene and TMPTA and assessed their catalytic efficiency in glycerol acetylation. A factorial design was employed to study the effects of cross-linker percentage and cross-linker feed time. Lower cross-linker percentages resulted in higher catalytic efficiencies, and there was a linear correlation between catalytic efficiency and cross-linker percentage. Statistical and kinetic models were developed to represent the experimental results and support resin formulation improvement strategies. The TMPTA feed time positively influenced catalytic efficiency, indicating the importance of resin micro- and macrostructure.
Article
Biochemistry & Molecular Biology
Ana F. Costa, Emanuel Senra, Isabel Faria-Ramos, Andreia Teixeira, Joao Morais, Mariana Pacheco, Celso A. Reis, Catarina Gomes
Summary: Expression of SLeX is associated with malignant transformation of cancer cells and their invasive properties. In this study, the role of ST3GalIV in the biosynthesis of SLeX and malignant properties of GI cancer cells was investigated. The findings suggest that ST3GalIV is the main enzyme driving the biosynthesis of SLeX and affects cancer cell motility.
GLYCOCONJUGATE JOURNAL
(2023)
Article
Engineering, Chemical
Mohsen Karimi, Alexandre Ferreira, Alirio E. Rodrigues, Farid Nouar, Christian Serre, Jose A. C. Silva
Summary: The microporous bioderived Al dicarboxylate MIL-160(Al) MOF in its shaped form has shown potential as a candidate for biogas upgrading (BU) and/or carbon capture and storage (CCS). The adsorption isotherms of CO2, CH4, and N2 at different temperatures and pressures were studied, revealing the loading capacities and fitting with the dual-site Langmuir model. Uptake rate experiments and Response Surface Methodology (RSM) were used to calculate the driving force coefficients and maximize selectivity for CO2/CH4 and CO2/N2 mixtures. Breakthrough curve experiments were simulated using ASPEN Adsorption, confirming the potential of MIL-160(Al) for BU and/or CCS applications.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Engineering, Chemical
Luana P. Queiroz, Carine M. Rebello, Erbet A. Costa, Vinicius V. Santana, Bruno C. L. Rodrigues, Alirio E. Rodrigues, Ana M. Ribeiro, Idelfonso B. R. Nogueira
Summary: In the past decades, the flavor industry has increased its investment in research and development to take innovative steps. However, the lack of information about flavored molecules and specific flavoring properties hinders progress in this sector. This study presents the use of three scientific machine learning techniques as an innovative methodology to design new natural flavor molecules with specific desired properties for product development. The transfer learning technique is utilized to address the lack of available data when analyzing flavor molecules. Nine flavor descriptors were studied, with more than 50% of molecules generated achieving outstanding results in evaluation metrics such as natural product-likeness score and synthetic accessibility score. Finally, a discussion on the results considers data availability, presence in nature, and the impact of multisensory flavor components on specific flavor outcomes.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Engineering, Chemical
Mariana C. N. Bessa, Azahara Luna-Triguero, Jose M. Vicent-Luna, Paulo M. O. C. Carmo, Mihalis N. Tsampas, Ana Mafalda Ribeiro, Aliirio E. Rodrigues, Sofia Calero, Alexandre F. P. Ferreira
Summary: Two industrial dual-step pressure swing adsorption (PSA) processes were designed and simulated to obtain high-purity methane, CO2, and syngas from a gas effluent of a CO2 electroreduction reactor using different design configurations. NaX and MFI zeolites were selected from the investigated set for simulation. Case study 1 achieved 90.5% methane purity with a 95.2% recovery, whereas case study 2 obtained methane with a 97.5% purity and 95.3% recovery. Both case studies produced high-purity CO2 (>97%) and syngas with a H-2/CO ratio above 4. However, case study 2 had a significantly higher energy consumption compared to case study 1 (64.9 vs 29.8 W h mol(CH4) (-1)).
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Ana Rita Pereira, Carina Costa, Nuno Mateus, Victor de Freitas, Alirio Rodrigues, Joana Oliveira
Summary: Large amounts of vine shoots generated during vine pruning still contain compounds from the original plant, including phenolic compounds and structural compounds like cellulose, hemicellulose, and lignin. This study proposes the extraction of lignin from vine shoots for the production of nanoparticles, using ethanol/toluene and water/ethanol as solvents. The lignin nanoparticles showed excellent antioxidant properties and the extracts from biomass pretreatment also exhibited antioxidant activity. Overall, this work contributes to the integral reuse of vine shoot byproduct, promoting sustainability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Juliana Pocas, Catarina Marques, Catarina Gomes, Andreia Hanada Otake, Filipe Pinto, Mariana Ferreira, Tiago Silva, Isabel Faria-Ramos, Rita Matos, Ana Raquel Ribeiro, Emanuel Senra, Bruno Cavadas, Silvia Batista, Joana Maia, Joana A. Macedo, Luis Lima, Luis Pedro Afonso, Jose Alexandre Ferreira, Lucio Lara Santos, Antonio Polonia, Hugo Osorio, Mattias Belting, Celso A. Reis, Bruno Costa-Silva, Ana Magalhaes
Summary: Gastric cancer is influenced by the expression of syndecan-4 (SDC4) which is associated with poor patient survival. SDC4 plays a crucial role in regulating the motility and invasion of gastric cancer cells. Additionally, SDC4 decorated with heparan sulfate is involved in the distribution, uptake, and functional effects of extracellular vesicles (EVs) derived from gastric cancer cells. SDC4 knockout disrupts the tropism of EVs for common gastric cancer metastatic sites. These findings provide insight into the molecular implications of SDC4 expression in gastric cancer cells and present potential therapeutic strategies targeting the glycan-EV axis to limit tumor progression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Engineering, Chemical
Marcio A. F. Martins, Alirio E. Rodrigues, Jose M. Loureiro, Ana M. Ribeiro, Idelfonso B. R. Nogueira
Summary: This paper addresses the application of a robust model predictive control strategy to a pressure swing adsorption unit. Linear models are used to handle uncertainties and describe the nonlinear behavior of the unit. The control law incorporates a multi-model uncertainty description and a tracking scheme for steady states.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Alvaro M. Martins, Tania M. Lopes, Francisca Diniz, Jose Pires, Hugo Osorio, Filipe Pinto, Daniela Freitas, Celso A. Reis
Summary: Alterations in glycosylation machinery in cancer cells lead to the synthesis of abnormal glycan structures in tumor cells. Extracellular vesicles (EVs) are involved in cancer communication and progression, and certain tumor-associated glycans have been identified in cancer EVs. This study investigates the impact of 3D tumor architecture on the selective packaging of cellular glycans into EVs. The results show differential packaging of specific proteins and glycans in EVs produced by 2D and 3D cultured cells, suggesting distinct biological functions and correlation with clinical data.
Article
Engineering, Chemical
Albertina G. Rios, Ana M. Ribeiro, Aliïrio E. Rodrigues, Alexandre F. P. Ferreira
Summary: The separation of bovine serum albumin and myoglobin was achieved using ion-exchange simulated moving bed. The study demonstrates the possibility of obtaining high purity, recovery, and productivity under specific operating conditions.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Engineering, Chemical
Rute Seabra, Rafael O. M. Dias, Maria J. Regufe, Ana Mafalda Ribeiro, Alirio E. Rodrigues, Alexandre F. P. Ferreira
Summary: This study focused on the separation of propane and propylene using carbon dioxide as a desorbent and binder-free zeolite 13X as an adsorbent. The gas-simulated moving bed (SMB) technology was utilized at a mild temperature (323 K). The adsorption equilibrium isotherms were measured, and breakthrough experiments were conducted to obtain binary adsorption equilibrium data. The experiment demonstrated the production of high-purity propylene with a high recovery using binder-free zeolite 13X and carbon dioxide as the desorbent at a mild temperature (323 K).
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Environmental Sciences
P. Mourao, C. Cassavela, I. Cansado, J. Castanheiro, L. Ribeiro, F. Pagnanelli
Summary: In this study, granulated samples were prepared to enhance the adsorption capacity of bisphenol A. The samples were characterized structurally and chemically, and the results showed that stable granulated adsorbents with good bisphenol A adsorption capacity can be prepared using different commercial silica binders.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Luis M. C. Oliveira, Vinicius V. Santana, Alirio E. Rodrigues, Ana M. Ribeiro, Idelfonso B. R. Nogueira
Summary: This study developed a framework based on scientific machine learning strategies and utilized transfer learning to predict odor thresholds for chemical substances. Results showed that the transfer learning-based strategy displayed better predictive performance.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)