Review
Biotechnology & Applied Microbiology
Jorge Joa, Joao Lampreia, Duarte Miguel F. Prazeres, Ana M. Azevedo
Summary: Bacteriophages, as the most abundant biological entities on Earth, have a high level of specificity in infecting bacteria and offer a solution to the emergence of multidrug-resistant bacteria. The development of phage therapies should be accompanied by improvements in phage biomanufacturing processes. Key factors in phage production and purification, traditional purification methods, and state-of-the-art purification approaches are discussed, with continuous purification of phages presented as a future trend in biomanufacturing.
BIOTECHNOLOGY ADVANCES
(2021)
Review
Biochemical Research Methods
Danish J. Malik
Summary: A successful future bacteriophage industry requires robust and scalable manufacturing platforms, with a quality-by-design approach for cost-effectiveness, and the use of process analytical tools for monitoring and control. The development of biophysical characterization tools for phage can have a significant impact on the prospects of the industry.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Review
Polymer Science
Evgenia Korzhikova-Vlakh, Mariia Antipchik, Tatiana Tennikova
Summary: This review provides a comprehensive summary of the current achievements in the preparation and application of macroporous polymer monolithic layers, as well as their utility as solid phases in thin-layer chromatography and various microarray technologies.
Article
Virology
Frej Larsen, Simone Margaard Offersen, Viktoria Rose Li, Ling Deng, Dennis Sandris Nielsen, Torben Solbeck Rasmussen
Summary: This study investigates the impact of ultrafiltration on viral extraction and the resulting viral community. The choice of ultrafilter is found to affect the recovery of different phage strains in a sample-dependent manner.
Article
Biochemical Research Methods
Masaru Kato, Misa Yamaguchi, Tomoka Morita, Noriko Watanabe, Shigenori Ota, Eiichi Yamamoto
Summary: In this study, three types of cationic modified silica monoliths were used to separate and purify nanoparticles, and the nanoparticles were separated from other substances by changing the pH and concentration of the eluent. The results showed that this method can extract nanoparticles in a short time with only low-speed centrifugation and has a high recovery efficiency.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemistry & Molecular Biology
Julia Neumair, Claudia D'Ercole, Matteo De March, Martin Elsner, Michael Seidel, Ario de Marco
Summary: In this study, a new affinity chromatography platform composed of a macroporous epoxy monolith matrix functionalized with anti-CD63 nanobodies was developed. The platform successfully captured and released integral EVs from urine samples with negligible unspecific binding for circulating proteins. Size discrimination of eluted EVs was achieved by different elution approaches. The use of inexpensive recombinant nanobodies and scalable monolith materials make this platform feasible and affordable for large-scale production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Yueyue Liu, Hui Wang, Haoyuan Yang, Zixuan Wang, Zhenxin Huang, Denghui Pan, Zhuangfei Zhang, Zhiyong Duan, Tingting Xu, Dezhi Kong, Xinjian Li, Ye Wang, Jingyu Sun
Summary: Researchers have developed a three-dimensional porous hierarchical silver/reduced graphene oxide microlattice aerogel as a sodiophilic monolith. The electrode exhibits excellent sodium ion flux and uniform deposition kinetics, resulting in long cycling lifespan and high Coulombic efficiency, making it a promising candidate for high-capacity sodium metal anodes.
Article
Biochemical Research Methods
Lucija Rebula, Andrej Raspor, Mojca Bavcar, Ales Strancar, Maja Leskovec
Summary: Bacteriophages have great potential as therapeutic agents, particularly in human therapy and antibiotic replacement. However, phage lysates may contain harmful by-products, making purification crucial. This article presents a two-step chromatographic purification method for P. aeruginosa bacteriophage, achieving high recovery rates and reducing contaminants. An HPLC analytical method was also developed for accurate measurement and analysis of phage concentration and impurities.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Physics, Applied
Kazuya Okamoto, Kodai Kishibe, Naoki Sano, Katsuaki Tanabe
Summary: We propose and demonstrate a new concept of semiconductor wafer bonding that combines bond formation and solar cell implementation. We develop a semiconductor bonding technique using PEDOT:PSS as a mediator. By utilizing this technique, we create an InP/Si heterostructure with a PEDOT:PSS/Si heterojunction at the bonded interface, which functions as a photovoltaic device. The prepared InP/PEDOT:PSS/Si heterostructure serves as a prototype for a multijunction solar cell with a built-in subcell, and our semiconductor bonding scheme could potentially enable low-cost, high-throughput production of high-efficiency multijunction solar cells.
APPLIED PHYSICS EXPRESS
(2023)
Review
Chemistry, Physical
Jinjue Zeng, Chenyang Xu, Tian Gao, Xiangfen Jiang, Xue-Bin Wang
Summary: This review discusses the integration of two-dimensional nanosheets into three-dimensional porous monoliths, creating 3D graphene structures that prevent aggregation or restacking of individual graphene sheets and provide pathways for electron/phonon transport and mass transfer. The synthesis methodology and applications of 3D graphene for electric double-layer capacitors are highlighted, along with current challenges and future prospects for manufacturing and application.
Article
Chemistry, Analytical
Marco Wilke, Bettina Roeder, Martin Paul, Michael G. Weller
Summary: A novel stationary phase for affinity separations based on sintered borosilicate glass is presented, showing fast binding kinetics and long-term stability. The material can be easily modified for selective binding, and columns and holders can be manufactured by 3D printing. High separation speed and pressure resistance allow for high productivity and purity of IgG purification with standard HPLC systems.
Article
Multidisciplinary Sciences
Rudy Plarre, Andrea Zocca, Andrea Spitzer, Sigrid Benemann, Anna A. Gorbushina, Yuexuan Li, Anja Waske, Alexander Funk, Janka Wilbig, Jens Guenster
Summary: The frass of larvae of the European house borer and drywood termites was tested as a natural feedstock for 3D-printing of wood-based materials. The filigree-like particles flow easily permitting the build-up of wood-based structures in a layer wise fashion using the Binder Jetting printing process.
Article
Microbiology
Ireneusz Sobolewski, Katarzyna Adamowicz, Anna Struck, Agnieszka Zylicz-Stachula, Piotr M. Skowron
Summary: DNA purification methods are important tools in molecular biology, but currently available techniques may not be effective for certain specialized applications. We have developed a method that uses silica columns and mini-scale isolation to efficiently purify bacteriophage genomic DNA from concentrated phage preparations, without the need for removing CsCl solutions.
Article
Physics, Applied
Henry T. Aller, Alan J. H. McGaughey, Jonathan A. Malen
Summary: Rapid thermal annealing significantly improves the thermal conductivity of amorphous alumina thin films and reduces the thermal boundary resistance of adjacent interfaces, which promotes heat spreading and enhances device longevity.
APPLIED PHYSICS LETTERS
(2023)
Article
Chemistry, Physical
A. P. Shivprasad, S. C. Vogel, V. K. Mehta, M. W. D. Cooper, T. A. Saleh, J. T. White, J. R. Wermer, E. P. Luther, H. R. Trellue
Summary: Yttrium dihydride has been successfully prepared in high-density monoliths using powder metallurgical methods in this study. The measured and calculated physical properties of yttrium dihydride confirm the feasibility and high quality of this method for large-scale production.
JOURNAL OF ALLOYS AND COMPOUNDS
(2021)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)