Article
Biochemical Research Methods
Sofia Albolafio, Alicia Marin, Maria I. Gil
Summary: In this study, a method for the quantification of HAAs in fresh-cut lettuce and process water samples was optimized and successfully applied. The UHPLC-MRM analysis showed good selectivity, specificity, and linearity, making it suitable for quality control and regulatory analysis in the fruit and vegetable industry.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Agriculture, Dairy & Animal Science
Holger A. Ebhardt, Pierre Ponchon, Konstantinos Theodosiadis, Christophe Fuerer, Marie -Claude Courtet-Compondu, Jonathan O'Regan, Michael Affolter, Yousef Joubran
Summary: High resolution mass spectrometry can identify hundreds to thousands of proteins per sample with label-free quantification, but the gold standard for quantitative proteomics is MRM. The authors demonstrate a reductionist approach using correlation analysis of dairy ingredients, successfully explaining variance in proteomics data with only 9 proteins. Their method, Trinity-MRM, shows reproducibility below 5% in independent experiments with double cream.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Biochemical Research Methods
Claudia Gaither, Robert Popp, Sophia P. Borchers, Kjartan Skarphedinsson, Finnur F. Eiriksson, Margret Thorsteinsdottir, Yassene Mohammed, Christoph H. Borchers
Summary: The study demonstrated that leaving a lyophilized peptide mixture at room temperature for up to 20 days does not affect the quantitative performance of mass spectrometry analysis, making it a feasible option for sample transportation. Most peptide concentrations showed minimal fluctuations across different time points, indicating the stability of the peptide mixture under the tested conditions.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Agriculture, Multidisciplinary
Mengmeng Wang, Bowen Yang, Ping Shao, Fan Jie, Xuan Yang, Baiyi Lu
Summary: Dietary intake of plant sterols reduces cholesterol levels in serum and liver, but increases cholesterol levels in the brain, while intake of cholesterol oxidation products increases levels of all COPs in serum.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Maura Brioschi, Erica Gianazza, Piergiuseppe Agostoni, Beatrice Zoanni, Alice Mallia, Cristina Banfi
Summary: This study used a targeted mass spectrometry approach to analyze plasma samples from HF patients and healthy controls, identifying a panel of four plasma proteins associated with HF severity and pulmonary dysfunction, with the potential to distinguish between HF patients and healthy subjects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Agriculture, Multidisciplinary
Chuanling Deng, Mingxuan Li, Yang Liu, Chi Yan, Zouyan He, Zhen-Yu Chen, Hanyue Zhu
Summary: Cholesterol oxidation products (COPs) are substances formed during food processing that can affect human health and increase the risk of various diseases. Preventing the generation of COPs in foods is crucial, and natural antioxidants as well as processing measures can help inhibit their production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemical Research Methods
Dongyoon Shin, Sang Jin Rhee, Jihyeon Lee, Injoon Yeo, Misol Do, Eun-Jeong Joo, Hee Yeon Jung, Sungwon Roh, Sang-Hyuk Lee, Hyeyoung Kim, Minji Bang, Kyu Young Lee, Jun Soo Kwon, Kyooseob Ha, Yong Min Ahn, Youngsoo Kim
Summary: This study successfully differentiated major depressive disorder (MDD) from bipolar disorder (BD) using a targeted proteomic approach, developing a model composed of nine proteins with good discriminative ability. The proteins identified were associated with neuro, oxidative/nitrosative stress, and immunity/inflammation-related biological functions.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemical Research Methods
Thomas Alexandre Brunet, Sophie Ayciriex, Delphine Arquier, Jerome Lemoine, Jerome Randon, Arnaud Salvador
Summary: The field of metabolomics based on mass spectrometry has grown considerably in recent years due to the need to detect and quantify a large number of metabolites simultaneously. The current targeted multiplexed analysis methods based on LCMS/MS rely on absolute retention times and are not easily transferable between instruments. To address this, a new acquisition mode called scout-triggered MRM (stMRM) is introduced, which remains robust even in the presence of retention time shifts.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Chemistry, Analytical
Juntong Wei, Xiaojun Xiao, Kun Li, Yuanyuan Song, Shau-Ku Huang, Zongwei Cai, Zhigang Liu
Summary: This study utilized a derivatization strategy to improve the mass spectrometry analysis efficiency of MLFAs, providing a new approach for discovering disease biomarkers.
Article
Biochemistry & Molecular Biology
Akash Kumar Bhaskar, Salwa Naushin, Arjun Ray, Praveen Singh, Anurag Raj, Shalini Pradhan, Khushboo Adlakha, Towfida Jahan Siddiqua, Dipankar Malakar, Debasis Dash, Shantanu Sengupta
Summary: Lipid compositions are complex and challenging to identify, necessitating new comprehensive analysis methods. This study proposes a targeted-mass spectrometry method that can rapidly screen a large number of lipid species, and identifies alterations in plasma lipids due to vitamin B(12) deficiency.
Article
Biochemistry & Molecular Biology
Yoshihiro Kita, Suzumi M. Tokuoka, Yoshiya Oda, Takao Shimizu
Summary: This article reports the development of a lightweight chromatogram browser software called TRACES for MRM-based targeted LC-MS analysis. TRACES provides rapid access to MRM chromatograms and has a chromatogram-level deisotoping function, helping users with data browsing and identification of regions potentially affected by isotopic signals.
Article
Biochemistry & Molecular Biology
Weicheng Wu, Jingqiu Chen, Dandan Yu, Shiguo Chen, Xingqian Ye, Zhiguo Zhang
Summary: Different cooking methods have significant effects on the glucosinolate content in red cabbage, with microwaving and steaming being able to better retain glucosinolates. Boiling, microwaving, steaming, frying, and stir-frying resulted in reductions in glucosinolate levels.
Article
Food Science & Technology
Mengmeng Wang, Yan Liu, Tian Zhao, Fan Xiao, Xuan Yang, Baiyi Lu
Summary: This study reveals that phytosterols, COPs, and POPs have different effects on the composition and metabolism of glycerophospholipids, sphingolipids, and glycerolipids in the liver of ApoE(-/-) mice. Specifically, they impact liver lipid metabolism and atherosclerosis risk factors differently.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Hui Tan, Shen-An Wang, Zixi Yan, Jianzhong Liu, Jialiang Wei, Song Song, Ning Jiao
Summary: This study demonstrates a novel N-heterocyclic carbene (NHC) catalyzed oxidative transformation of organic halides into esters utilizing O-2 as the oxygen source. Mechanistic studies show the oxidation of deoxy Breslow intermediate to Breslow intermediate for further transformation. This method expands the field of NHC catalysis and promotes oxygenation reactions with O-2.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Environmental Sciences
Maxime Lepretre, Olivier Geffard, Anabelle Espeyte, Julien Faugere, Sophie Ayciriex, Arnaud Salvador, Nicolas Delorme, Arnaud Chaumot, Davide Degli-Esposti
Summary: This study utilized multiple reaction monitoring mass spectrometry to measure customized molecular markers in freshwater sentinel species and proposed a breakthrough approach to screen and identify potential proteins.
ENVIRONMENTAL POLLUTION
(2022)
Article
Cell Biology
Xiaojuan Liang, Cong Tao, Jianfei Pan, Lilan Zhang, Lulu Liu, Ying Zhao, Yiping Fan, Chunwei Cao, Jiali Liu, Jin Zhang, Sin Man Lam, Guanghou Shui, Wanzhu Jin, Wei Li, Jianguo Zhao, Kui Li, Yanfang Wang
Summary: The adipocyte-specific knockout of RNF20 in mice resulted in progressive fat loss, organomegaly, and hyperinsulinemia, leading to severe liver steatosis in high-fat diet-fed mice. Despite signs of hyperinsulinemia, normal insulin sensitivity and improved glucose tolerance were observed in the knockout mice. Reduced expression of BAT selective genes and impaired mitochondrial functions were observed, highlighting the critical role of RNF20 in adipose tissue development and physiological function.
Article
Biotechnology & Applied Microbiology
Guanlan Xing, Jinyu Li, Wenli Li, Sin Man Lam, Hongli Yuan, Guanghou Shui, Jinshui Yang
Summary: This study identified AP2 superfamily and R2R3-MYB family genes in the green algae A. protothecoides and analyzed their expression profiles under temperature stress. It found intrinsic associations between these transcription factors and the regulatory mechanism of lipid metabolism, providing insights into their roles in lipid adjustments and response to temperature stress. These findings contribute to biotechnological development in microalgae-based biofuel production and enhance understanding of photosynthetic organisms' adaptive mechanism to temperature stress.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biology
Qianqian Pang, Chunyi Liu, Yulong Qiao, Jian Zhao, Sin Man Lam, Mei Mei, Guanghou Shui, Shilai Bao, Qiuling Li
Summary: Deletion of the GM130 gene disrupts the Golgi structure, impairing the secretion of lung surfactant proteins and lipids. This leads to an enlargement of alveolar airspace and increased alveolar epithelial autophagy, which can be partially rescued by surfactant repletion in GM130-deficient mice.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Environmental Sciences
Chao Xing, Jinyu Li, Sin Man Lam, Hongli Yuan, Guanghou Shui, Jinshui Yang
Summary: This study revealed that under Cd stress, glutathione can activate TAG synthesis in A. protothecoides, leading to TAG accumulation and glutathione accumulation, which can enhance resistance to high Cd stress. Insights into a new operation mode of TAG accumulation under heavy metal stress were provided.
JOURNAL OF ENVIRONMENTAL SCIENCES
(2021)
Article
Plant Sciences
Yingchun Wang, Jianan Zhai, Zhenyu Qi, Wanping Liu, Jipeng Cui, Xi Zhang, Sulan Bai, Li Li, Guanghou Shui, Suxia Cui
Summary: Physcomitrella patens, an early terrestrial plant, has short-term desiccation resistance, with membrane integrity maintenance related to its specific glycerolipid composition and key genes for lipid metabolism. Phospholipids and glycolipids dominate its lipid composition, with phospholipids accounting for 61.7% and glycolipids for 26.5%. The study sheds light on lipid evolution of early terrestrial plants in adverse terrestrial environments.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Plant Sciences
Mingjian Hu, Haiming Zhao, Bo Yang, Shuang Yang, Haihong Liu, He Tian, Guanghou Shui, Zongliang Chen, E. Lizhu, Jinsheng Lai, Weibin Song
Summary: The maize kernel development is influenced by the choline transporter-like protein ZmCTLP1, which affects kernel size, starch content, membrane lipid homeostasis, wall in-growth formation, and plasmodesmata development in transfer cells. The mutation of ZmCTLP1 leads to reduced choline content and incomplete differentiation of transfer cells, indicating its crucial role in maize kernel development.
Article
Oncology
Mingjun Cao, Shaohua Zhang, Sin Man Lam, Guanghou Shui
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Ying Yu, Bin Liu, Xiaolin Liu, Xuan Zhang, Wenhui Zhang, He Tian, Guanghou Shui, Wenzhao Wang, Moshi Song, Jun Wang
Summary: The gut-liver axis refers to the complex connection and interaction between the gut microbiome and the liver, which affects the host metabolism. The hepatic portal vein is considered to be the main route for metabolite transportation in the gut-liver axis, but the existence and importance of other routes are still unclear. By comparing the metabolites in the blood circulation and mesenteric lymph systems, significant shifts in metabolites in serum and mesentery are identified. The study reveals that the lymph system has the potential to regulate liver metabolism by transporting certain metabolites independently.
JOURNAL OF GENETICS AND GENOMICS
(2022)
Review
Endocrinology & Metabolism
Sin Man Lam, Xun Huang, Guanghou Shui
Summary: This review discusses the connection between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and systemic lipid couriers, such as high-density lipoproteins (HDLs) and exosomes, in relation to the neurological aspects of the infection. It is found that SARS-CoV-2 primarily targets lipid-producing cells and utilizes host cell lipid metabolism for efficient replication and systemic spreading. Additionally, certain lipid particles have the ability to traverse the blood-brain barrier (BBB) and contribute to the neurotropism of SARS-CoV-2.
TRENDS IN ENDOCRINOLOGY AND METABOLISM
(2022)
Article
Chemistry, Physical
He Tian, Zhen Ni, Sin Man Lam, Wenxi Jiang, Fengjuan Li, Jie Du, Yuan Wang, Guanghou Shui
Summary: This study develops a robust metabolomics approach for accurate quantitation and precise profiling of metabolomes. By introducing isotope-labeled standards (ISs) and refining the selection of appropriate ISs, this approach successfully reduces COVs and facilitates the identification of additional metabolites. The approach reveals perturbations in various metabolic pathways across aging, providing clues for the prevention and treatment of age-related diseases.
Article
Cell Biology
Xinyu Bao, Xiaogen Ma, Rongfeng Huang, Jianghui Chen, Haoran Xin, Meiyu Zhou, Lihua Li, Shifei Tong, Qian Zhang, Guanghou Shui, Fang Deng, Liqing Yu, Min-Dian Li, Zhihui Zhang
Summary: This study demonstrates that inhibiting Plin3 in hepatocytes is sufficient to alleviate hepatic steatosis and steatohepatitis caused by CGI-58 deficiency.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Hematology
Jing-gang Xia, Bowen Li, Hao Zhang, Qin-xue Li, Sin Man Lam, Chun-lin Yin, He Tian, Guanghou Shui
Summary: This study used quantitative metabolomics to investigate the metabolic differences in acute myocardial infarction (AMI) patients with and without diabetes. The findings revealed that diabetes-AMI patients had more severe metabolic dysregulation, involving multiple pathways such as carbohydrate metabolism, lipid metabolism, glycolysis, tricarboxylic acid cycle, and amino acid metabolism. The key functional metabolites N-lactoyl-phenylalanine and lysophosphatidylcholines were identified, which were associated with mitochondrial dysfunction, glucose utilization, and inflammation, suggesting their potential as therapeutic targets for diabetes-AMI.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jinglin Zhu, Wei Meng, Sin Man Lam, Guanghou Shui, Xun Huang
Summary: By regulating lysosomal activity, the protein SPIN-4 ensures the synthesis of B12-associated PC, which is essential for germline development and fertility in Caenorhabditis elegans. Sterility resulting from PC deficiency can be rescued by reducing levels of polyunsaturated fatty acids, reactive oxygen species, and redox-active iron, indicating that it is mediated by germline ferroptosis. These findings highlight the critical role of PC homeostasis in ferroptosis susceptibility and provide a new target for pharmacological interventions.
JOURNAL OF GENETICS AND GENOMICS
(2023)
Review
Chemistry, Analytical
Zehua Wang, Mingjun Cao, Sin Man Lam, Guanghou Shui
Summary: Technological innovations have revolutionized the way scientists approach biological and clinical questions, shifting the focus from conventional bulk assays to the measurement of single molecules in individual cells. Single-cell lipidomics has emerged as a promising technique to provide unique insights into the lipid composition of individual cells. This review summarizes the current progress and challenges in single-cell lipidomics, particularly emphasizing the importance of accurate lipid identification and quantification, and discusses its potential applications in biology and clinical medicine.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Cell Biology
Cen Yan, Xiaojuan Ma, Sin Man Lam, Yuejie Zhang, Yu Cao, Yuan Dong, Li Su, Guanghou Shui, Yingmei Feng
Summary: GLP-1 RAs can control blood sugar and inhibit inflammation and plaque development. The study shows that Ex-4 can inhibit skewed myelopoiesis caused by hypercholesteremia, reducing the risk of atherosclerosis.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2023)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
