Article
Biochemical Research Methods
Quentin Gros, Jeremy Molineau, Angeline Noireau, Johanna Duval, Takeshi Bamba, Eric Lesellier, Caroline West
Summary: Achiral packed column supercritical fluid chromatography (SFC) has regained interest, leading to the design of specific stationary phases by major instrument manufacturers. SFC stationary phases have been extensively studied using linear solvation energy relationships (LSER), providing insights into retention mechanisms and facilitating comparisons. Through the use of descriptors like Abraham descriptors and shape features descriptors, the retention mechanisms and interaction capabilities of different stationary phases in SFC can be better understood.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Katarzyna Krzeminska, Szymon Bocian, Robert Pluskota, Boguslaw Buszewski
Summary: This study examined the chromatographic properties of six non-commercially available stationary phases with ester or phosphodiester functional groups. The zeta potential values of the stationary phases in different solvents were measured, and selectivity coefficients were calculated based on retention factor. Comparison was made between the hydrophobic and polar properties of the investigated columns.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Sanka N. Atapattu
Summary: The solvation parameter model system constants and retention factors were used to interpret the retention properties of calibration compounds on a biphenylsiloxane-bonded stationary phase. The study compared the retention properties of acetone-water binary mobile phases with acetonitrile and methanol mobile phases. The results showed that acetone mobile phases have similar retention properties to acetonitrile but with selectivity differences that can be exploited in reversed-phase liquid chromatography method development on the stationary phase.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Biochemical Research Methods
Pan Luo, Jingdong Peng, Huanjun Peng, Zilong Zhang, Jun Chen, Kun Fan, Xiang Wang
Summary: In this study, three regioisomeric ionic liquid silane reagents were synthesized and bonded to silica to fabricate new stationary phases. The retention behavior and mechanism of the new stationary phases were investigated, and it was found that they exhibited great retention repeatability and efficiency. The separation performances of the stationary phases towards different analytes varied depending on the position of imidazole ions.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Xiao-Dong Cheng, Zheng Zhang, Yun-Ping Li
Summary: Green chromatography techniques using low-toxic mobile phase and developing stationary phases with adequate retention and separation under high content water mobile phase have gained increasing attention. In this study, an undecylenic acid-bonded silica stationary phase (UAS) was successfully prepared using thiol-ene click chemistry. The synthesized UAS demonstrated excellent separation ability towards highly polar compounds under the mobile phase of high content water, surpassing commercial C18 and silica stationary phases. Overall, this UAS stationary phase meets the requirements of green chromatography and shows promise for enhanced separation.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Xiaoqing Wang, Jian Cui, Jin Zhou, Shuo Wang, Yiming Gu, Xiaowei Liu, Shudong Wang
Summary: Vinyl modified silica was synthesized through a silane coupling reaction and a poly-acrylamide modified silica (PAM-SIL) stationary phase was prepared via free radical polymerization. The separation performance of the stationary phases could be regulated by controlling the polymer structure, showing potential for future separation and analysis applications.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Review
Biochemical Research Methods
Yong Guo
Summary: Various polar stationary phases have been extensively used in HILIC applications in the past decade. This review provides an overview of commercially available polar stationary phases and summarizes the progress made in the past 5 years. New types of stationary phases are discussed, and the trend in stationary phase research and development is highlighted. The review also emphasizes the experimental evidence for direct interactions between polar analytes and stationary phase ligands in HILIC conditions, as well as the advancements in understanding the retention mechanisms.
BIOMEDICAL CHROMATOGRAPHY
(2022)
Article
Chemistry, Analytical
Sanka N. Atapattu
Summary: The solvation parameter model was used to investigate the intermolecular interactions that affect retention on a C18 stationary phase in the ACN:MeOH (1:1) solvent system. System maps were constructed and compared to evaluate the solvent strength of ACN:MeOH (1:1) in comparison to other organic modifiers. Solvation parameter models were successfully constructed for the ACN:MeOH (1:1)-water ternary solvent system, with high coefficient of determination values and low standard error of the estimate values.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Farideh Hosseinkhani, Luojiao Huang, Anne-Charlotte Dubbelman, Faisa Guled, Amy C. Harms, Thomas Hankemeier
Summary: This study systematically evaluated the effectiveness of polar stationary phases for untargeted metabolomics using hydrophilic interaction liquid chromatography (HILIC) columns. The ZIC-c HILIC column operated at neutral pH showed superior performance for different classes of compounds, better isomer separation, repeatability and high metabolic coverage. However, the retention of inorganic ions in plasma resulted in ion-suppression as part of the overall plasma matrix effect. HILIC was successful in separating plasma samples with different phenotypes and can be used for comprehensive analysis of plasma samples and discovering metabolic changes associated with health and disease.
Article
Biochemical Research Methods
Honorine Lardeux, Davy Guillarme, Valentina D'Atri
Summary: Hydrophilic interaction chromatography (HILIC) is proposed as an alternative to ion-pairing reversed-phase chromatography (IP-RPLC) for oligonucleotide (ON) analysis. This study evaluates the potential of seven zwitterionic HILIC columns and other columns for ON analysis, and highlights the importance of understanding column characteristics (retention and selectivity) when selecting a stationary phase for specific ON applications.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Lidia Redon, Xavier Subirats, Marti Roses
Summary: The study used pycnometric and homologous series retention methods to investigate the volume and mean composition of water-rich layers partially adsorbed on the surface of HILIC column fillings with acetonitrile-water and methanol-water as eluents. The findings suggest that water is preferentially adsorbed on the surface of the HILIC bonded phase in hydroorganic eluents with high acetonitrile or methanol concentrations, forming several water-rich transition layers. In eluents with low acetonitrile or methanol concentrations, the amount of preferentially adsorbed water is minimal, and the retention behavior is similar to that of reversed-phase liquid chromatography.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Sanka N. Atapattu
Summary: The solvation parameter model was used to evaluate selectivity on siloxane-bonded reversed phase columns with acetone-water mobile phases. The study found that system constants showed higher similarity between acetone and acetonitrile, indicating easier method transferability.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Elham Sadat Hosseini, Kourosh Tabar Heydar
Summary: The research synthesized two acridine derivatives and developed two mixed-mode stationary phases by functionalizing silica. These stationary phases were designed with multiple interactions, including pi-pi stacking interaction, reverse phase, hydrophilic interaction, and anion exchange, based on the structure of the acridine groups on the silica gel. The performance of the columns was investigated by chromatographic parameters in terms of column efficiency, asymmetry factor, retention factor, and resolution.
Article
Biochemical Research Methods
Ting Huang, Wanru Zhang, Xiaoyun Lei, Huijuan Chen, Chenchen Lin, Xiaoping Wu
Summary: The novel POSS-based zwitterionic monolithic capillary column exhibits high hydrophilicity, electrostatic interaction, and good mechanical stability, allowing for tuneable electroosmotic flow across a wide pH range and separation of various polar compounds in capillary electrochromatography. It utilizes a mixed-mode hydrophilic interaction chromatography retention mechanism, including weak electrostatic interaction, hydrophobic interaction, and anion exchange.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Lidia Redon, Mahmoud Safar Beiranvand, Xavier Subirats, Marti Roses
Summary: In this study, the Abraham's solvation parameter model is revisited to develop a fast and reliable method for the characterization of columns used in reversed-phase liquid chromatography and hydrophilic interaction liquid chromatography. By carefully selecting pairs of compounds with similar molecular descriptors, except for one specific property, the selectivity of a chromatographic system can be inferred based on the differences in chromatographic retention.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Quentin Gros, Jeremy Molineau, Angeline Noireau, Johanna Duval, Takeshi Bamba, Eric Lesellier, Caroline West
Summary: Achiral packed column supercritical fluid chromatography (SFC) has regained interest, leading to the design of specific stationary phases by major instrument manufacturers. SFC stationary phases have been extensively studied using linear solvation energy relationships (LSER), providing insights into retention mechanisms and facilitating comparisons. Through the use of descriptors like Abraham descriptors and shape features descriptors, the retention mechanisms and interaction capabilities of different stationary phases in SFC can be better understood.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Review
Chemistry, Analytical
Jeremy Molineau, Maria Hideux, Caroline West
Summary: This paper reviews the application of liquid-phase chromatographic methods and emerging chromatographic techniques in pharmaceutical settings, focusing on the analysis and purification of biomolecules such as proteins and nucleic acids. Specific attention is placed on the use of supercritical fluid chromatography (SFC) and enhanced fluidity liquid chromatography (EFLC) for biomolecule analysis, including the potential benefits and detection issues like mass spectrometry hyphenation.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Analytical
Amandine Dispas, Adrian Clarke, Alexandre Grand-Guillaume Perrenoud, Luca Gioacchino Losacco, Jean-Luc Veuthey, Quentin Gros, Jeremy Molineau, Angeline Noireau, Caroline West, Fabio Salafia, Mariosimone Zoccali, Luigi Mondello, Amber Guillen, Jenny Wang, Kelly Zhang, Philipp Jochems, Gesa Schad, Kosuke Nakajima, Shinnosuke Horie, Jan Joseph, Maria Kristina Parr, Pierre Billemont, Antoni Severino, Sonja Schneider, Edgar Naegele, Daniel Kutscher, Rick Wikfors, Regina Black, Lee Ingvaldson, Jimmy Oliveira Da Silva, Raffeal Bennett, Erik L. Regalado, Thi Phuong Thuy Hoang, David Touboul, Yana Nikolova, Mariana Kamenova-Nacheva, Vladimir Dimitrov, Blair K. Berger, Kevin A. Schug, Solene Kerviel-Guillon, Fabien Mauge, Masatomo Takahashi, Yoshihiro Izumi, Takeshi Bamba, Florent Rouviere, Sabine Heinisch, Davy Guillarme, Philippe Hubert
Summary: The study evaluated the transferability and reproducibility of pharmaceutical impurity analysis using supercritical fluid chromatography (SFC) across different instrument brands. Results showed significant reproducibility differences between instruments, but overall reproducibility was comparable to LC methods.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemical Research Methods
Jeremy Molineau, Yasmine Hamel, Maria Hideux, Philippe Hennig, Sophie Bertin, Fabien Mauge, Eric Lesellier, Caroline West
Summary: A method for analyzing short-chain bioactive peptides and their impurities was developed using unified chromatography (UC), with optimal conditions identified on Ascentis Express OH5 stationary phase. This method was successfully applied to a large set of peptides, providing adequate scattering of peaks in the retention space and some separation of isomeric species, especially for cyclic peptides.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Jeremy Molineau, Maria Hideux, Philippe Hennig, Sophie Bertin, Fabien Mauge, Eric Lesellier, Caroline West
Summary: A unified chromatography (UC) analysis method was developed for short-chain peptides, and compared to a reference reversed-phase ultra-high-performance liquid chromatography (RP-UHPLC) method for the analysis of 43 peptides. The two methods showed differences in orthogonality, success rate, and performance, with excellent complementarity observed in impurity profiling and separation of isomeric species.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Chemistry, Analytical
Eric Lesellier, Caroline West
Summary: Plant-derived natural dyes are utilized in various products for their color and bioactivity. Supercritical fluid chromatography is used for analyzing these dyes, with different stationary phases and mobile phase compositions preferred based on the dye's structure and polarity. Chromatographic analysis can provide information on solubility in supercritical fluids and stationary phase properties.
JOURNAL OF SEPARATION SCIENCE
(2022)
Review
Chemistry, Analytical
Quentin Gros, Johanna Duval, Caroline West, Eric Lesellier
Summary: The development of automatized hyphenated systems is attractive for avoiding sampling error, saving time, and reducing costs. Among these systems, on-line SFE-SFC allows efficient, quick, and eco-friendly extraction, separation, and detection of molecules. The principles of supercritical fluid extraction (SFE) and supercritical fluid chromatography (SFC) are discussed, along with comparisons of partially-supercritical on-line systems before examining the evolution of on-line SFE-SFC over the past decades. Different devices for better understanding coupling challenges and determining advantageous configurations are also examined.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Quentin Gros, Laurine Reset, Johanna Duval, Shinnosuke Horie, Yusuke Toyota, Yasuhiro Funada, Yoshihiro Hayakawa, Eric Lesellier, Caroline West
Summary: When starting method development in SFC, the choice of suitable stationary phases can be difficult due to the large number of options available. In this project, 16 columns with diverse stationary phases and polarities were compared to determine their performance. The ranking was based on multiple criteria including the number of eluted compounds, elution time, peak width, peak symmetry, and retention along the gradient time. Columns with high overall scores, such as bare silica gel, propanediol-bonded silica, and pentabromobenzyloxy-bonded silica, were found to be advantageous.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Jeremy Molineau, Maria Hideux, Philippe Hennig, Sophie Bertin, Fabien Mauge, Eric Lesellier, Caroline West
Summary: The chromatographic analysis of long-chain hydrophilic therapeutic peptides was explored using pressurized CO2 as the mobile phase. The optimal method was obtained on a Torus 2-PIC column with a gradient elution of 50-90% co-solvent in CO2. UV and mass spectrometry were used to assess the purity and resolution of the major peak and its impurities. Peak distortion was observed in some cases, and thorough examination of dilution solvent and injection volume was conducted to improve peak shape and impurity resolution.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Chemistry, Applied
Quentin Gros, Marta Wolniaczyk, Johanna Duval, Caroline West, Shinnosuke Horie, Yusuke Toyota, Yasuhiro Funada, Eric Lesellier
Summary: The composition and abundance of triglycerides in vegetable lipid samples were analyzed using ultra-high efficiency / low-pressure supercritical fluid chromatography coupled with mass spectrometry. A total of 94 different triglycerides were identified and quantified, including low-abundance triglycerides and structurally similar isomers. Hierarchical cluster analysis was used to compare the similarity between samples, allowing for comparison of samples from different vegetable sources and the impact of refining treatments on triglyceride content.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Biochemical Research Methods
Quentin Gros, Marta Wolniaczyk, Johanna Duval, Shinnosuke Horie, Yasuhiro Funada, Yoshihiro Hayakawa, Caroline West, Eric Lesellier
Summary: Plant seeds, especially milk thistle seeds, contain valuable nonpolar and polar compounds with potential biological activity. This study developed an online method called supercritical fluid extraction (SFE)-supercritical fluid chromatography (SFC) to extract and analyze both types of compounds. Different transfer modes were compared, and the challenges of analyzing nonpolar and polar compounds were discussed. The versatility of online SFE-SFC and its potential in analyzing both types of compounds in a single sample were explored.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Organic
Yoshinori Tokairin, Hiroyuki Konno, Angeline Noireau, Caroline West, Hiroki Moriwaki, Vadim A. Soloshonok, Cyril Nicolas, Isabelle Gillaizeau
Summary: A new approach was developed for the asymmetric synthesis of beta-phosphorus-containing alpha-amino acids by utilizing chiral Ni(ii) complexes to catalyze the Michael addition of dehydroalanine-Schiff base with phosphine oxides and dialkyl phosphites.
ORGANIC CHEMISTRY FRONTIERS
(2021)
Article
Chemistry, Multidisciplinary
Nicu-Cosmin Ostache, Marie-Aude Hiebel, Adriana-Luminita Finaru, Hassan Allouchi, Gerald Guillaumet, Franck Suzenet
Summary: This study presents a convenient design for the preparation of new pyrazolo[3,4-c]pyrazoles, employing hydrazine condensations and C-N Ullmann-type cross-coupling reactions. The chemoselective bromination of bipyrazoles followed by Suzuki-Miyaura cross-coupling reactions allowed for the synthesis of various modulated heterobicycles with high therapeutic impact.
Article
Chemistry, Multidisciplinary
Elena-Mirela Suceveanu, Luminita Grosu, Irina-Claudia Alexa, Adriana-Luminita Finaru
SCIENTIFIC STUDY AND RESEARCH-CHEMISTRY AND CHEMICAL ENGINEERING BIOTECHNOLOGY FOOD INDUSTRY
(2020)
Article
Chemistry, Multidisciplinary
AlMa Mihaela (Maciuca) Birtea, Andra Poloboc, Nicolae Catalin Tampu, Raluca Ioana Tampu, Oana-Irina Patriciu, Berengere Claude, Reine Nehme, Adriana Luminita Finaru
SCIENTIFIC STUDY AND RESEARCH-CHEMISTRY AND CHEMICAL ENGINEERING BIOTECHNOLOGY FOOD INDUSTRY
(2020)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)