Review
Environmental Sciences
Aikaterini A. Zompra, Styliani A. Chasapi, Matthew S. Twigg, Karina Salek, Ioannis Anestopoulos, Alex Galanis, Aglaia Pappa, Tony Gutierrez, Ibrahim M. Banat, Roger Marchant, Stephen R. Euston, Mihalis I. Panayiotidis, Georgios A. Spyroulias
Summary: Synthetic surfactants, commonly used in various industries, have a negative impact on the environment due to their synthetic preparation. As a result, there is a growing interest in exploring natural sources of surfactants, which can be produced through environmentally friendly processes. Microbial production of such chemicals, however, often results in crude materials composed of multiple complex bioproducts. Therefore, advanced analytical methods are required for the extraction, identification, and characterization of biosurfactants.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biochemical Research Methods
Hua Li, Ru Ding, Chen Chen, Zhixuan Tian, Hongnan Yin, Lieming Ding
Summary: A sensitive and reliable LC-MS/MS method was developed for the determination of befotertinib and its metabolite D-0865 in human plasma. The method was validated according to the Chinese Pharmacopeia Commission and ICH Harmonised Guideline for Bioanalytical Method Validation. It was successfully applied for the quantitation of befotertinib and D-0865 during the pharmacokinetics study of befotertinib in NSCLC patients.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
Sara Palmieri, Marcello Mascini, Eleonora Oliva, Eduardo Viteritti, Fabiola Eugelio, Federico Fanti, Dario Compagnone, Manuel Sergi
Summary: A new multitarget screening procedure using HPLC-MS/MS analysis was developed for the detection and identification of multiple cannabinoids in hemp. The results showed accurate classification of different cultivars and highlighted the limitations of traditional classification based on common cannabinoids.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Analytical
Ping Du, Lingling Xuan, Zhuoling An, Yuhui Zhang
Summary: MRTX849 is a novel targeted inhibitor of KRAS (G12C) that improves the response rate in patients with advanced solid tumors. This study reports an analytical HPLC-MS/MS assay and pharmacokinetics for MRTX849 in plasma, demonstrating the accuracy and validity of the method.
Article
Food Science & Technology
Fan Liu, Jan van der Molen, Folkert Kuipers, Sander S. van Leeuwen
Summary: Human milk is the best food for infants, while infant formulas are appropriate substitutes. This study analyzed the concentrations of bioactive components in different infant formulas available on the Dutch market and found that they were lower than in human milk.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biochemical Research Methods
Devan Loganathan, Rong Yi, Bhavesh Patel, Julia Zhang, Noel Kong
Summary: The importance of distinguishing between inadvertent ingestion of plant-based products and deliberate administration of designer synthetic analogs in equine anti-doping analysis is increasing. A rapid and sensitive method was developed and validated for the detection, resolution and quantitative determination of cathinone enantiomers in horse blood plasma and urine. This study is the first to successfully resolve and quantify cathinone enantiomers in horse blood plasma and urine at sub nanogram levels.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Environmental Sciences
Crystal L. Sweeney, Jessica L. Bennett, Clarke A. M. Brown, Neil W. Ross, Graham A. Gagnon
Summary: The QuEChERS method offers an alternative sample preparation approach for extracting estrogens from complex wastewater matrices, providing reliable quantitation of E1, E2, and E3. Excellent process efficiencies were observed for E2 and E1, while moderate to acceptable process efficiencies were achieved for E3, demonstrating the method's reliability and effectiveness in analyzing estrogens in waste systems.
Article
Chemistry, Analytical
Karel Kudlacek, Jacopo La Nasa, Erika Ribechini, Maria Perla Colombini, Karel Nesmerak
Summary: A multi-analytical approach was used for the first time to study six historical ointments from the 18th century of Capuchin Monastery in Prague, Czech Republic. The analysis involved gas chromatography, mass spectrometry, solid-phase microextraction, liquid chromatography, and flow injection analysis to identify ingredients and determine the chemical modifications. The results provided insights into the ointments' formulation and alteration of chemical compounds.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Applied
Utpal Bose, James A. Broadbent, Angela Juhasz, Shaymaviswanathan Karnaneedi, Elecia B. Johnston, Sally Stockwell, Keren Byrne, Vachiranee Limviphuvadh, Sebastian Maurer-Stroh, Andreas L. Lopata, Michelle L. Colgrave
Summary: The study explored the proteome of insect products using LC-MS/MS and identified potential allergens, particularly three proteoforms of arginine kinase. A targeted MS assay was developed to detect these allergens, showing variability in abundance/detectability based on extraction and processing methods.
Article
Medical Laboratory Technology
Shin Aleks, Connolly Shawn, Little Randie, Kabytaev Kuanysh
Summary: This study developed an assay for absolute quantitation of glycated albumin based on isotope dilution liquid chromatography-mass spectrometry. The method showed good analytical performance with a linear correlation between LC-MS/MS and Lucica method. This approach allows simultaneous quantitation of glycated and non-glycated peptides.
CLINICA CHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Niraj Shende, Abhijeet Karale, Prashant Bore, Sudhakar Bhagade, Ashishkumar Gulhane, Asha D. Mallya, Rajeev M. Dhere
Summary: Polysaccharide activation evaluation is crucial for conjugate vaccine quality. Pneumococcal polysaccharides of serotypes 5, 6B, 14, 19A, and 23F were cyanylated for 3 and 8 minutes, and their activation was assessed through methanolysis and derivatization. The results showed controlled conjugation kinetics in serotype 6B (22% and 27%) and serotype 23F Ps (11% and 36%) at 3 and 8 minutes, respectively. The GC-MS analysis of structural modifications provides important information for consistent conjugate vaccine manufacturing.
CARBOHYDRATE RESEARCH
(2023)
Article
Agriculture, Multidisciplinary
Qingmei Song, Ye Wang, Shouying Tang, Xiurou Meng, Fei Wang, Deyu Hu, Yuping Zhang
Summary: Four difenoconazole stereoisomers were separated and their configurations confirmed in a study. A trace detection method for residue determination in plant samples was developed using HPLC-MS/MS, showing high accuracy and recovery rates. The degradation of the four stereoisomers showed no obvious enantioselectivity, with one particular stereoisomer degrading the slowest in one region.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Food Science & Technology
Shouying Tang, Xiurou Meng, Yongkang Wang, Xueqin Shi, Tianyou Feng, Deyu Hu, Yuping Zhang
Summary: The study developed a method for determining two dimethacarb isomers in rice, showing that the half-lives of dimethacarb in rice straw ranged from 4.08 to 4.23 days in field experiments. The final residues of XMC and MPMC in rice husk after 21 days of spraying at six sites ranged from 0.23-2.65 mg/kg, while in brown rice the residues were lower.
Article
Engineering, Environmental
Lina Yang, Dehua Hu, Hailing Liu, Xiangfeng Wang, Yuan Liu, Qianshu Xia, Suimin Deng, Yun Hao, Yuhao Jin, Mengxia Xie
Summary: The immobilization of penicillinase into ZIF-8 enhanced the catalytic activity and stability, providing a promising strategy for degrading penicillin residues in water environment.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Chemistry, Analytical
Doaa M. Mustafa, Nancy Magdy, Noha F. El Azab
Summary: The study focused on the development of a stability-indicating HPLC-DAD technique for Vericiguat (VER) analysis. VER was found to be sensitive to alkaline, oxidative and thermal degradation. The analysis method was validated to be specific, fast, simple, precise and accurate for routine analysis and quality control of VER in its pharmaceutical formulation. The technique was also used to investigate the kinetics of alkaline, oxidative and dry heat degradation.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)