期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1218, 期 50, 页码 9064-9070出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2011.10.021
关键词
High-performance liquid chromatography; Chemiluminescence; Low molecular weight aminothiols; Triangular gold nanoparticles; Postcolumn reagents
资金
- National Natural Science Foundation of China [21077008, 20975010]
- 973 Program [2011CBA00503]
- Ministry of Education Foundation of Humanities and Social Sciences [10YJC630208]
Our recent study demonstrates the synthesized triangular gold nanoparticles (AuNPs) by trisodium citrate reduction of HAuCl(4) in the presence of nonionic fluorosurfactant (FSN) could display stronger catalytic activity towards luminol-chemiluminescence (CL) than spherical AuNPs. Ultratrace aminothiols may cause a great decrease in CL intensity of the triangular AuNPs-luminol CL system. In this article, we utilize the as-prepared triangular AuNPs as novel postcolumn CL reagents to explore a simple high-performance liquid chromatography (HPLC)-CL method for the determination of low molecular weight aminothiols (i.e., cysteine, homocysteine, glutathione, cysteinylglycine and glutamylcysteine). The as-prepared triangular AuNPs were easier to synthesize, stable at a wider pH range and high ionic strength, and highly selective and sensitive towards reduced aminothiols. The detection limits at a signal-to-noise ratio of 3 for cysteine, homocysteine, glutathione, cysteinylglycine and glutamylcysteine were 0.016, 0.08, 0.1, 0.04 and 0.1 pmol, respectively. Recoveries from spiked urine and plasma samples were 95.7-104.3%. The applicability of the proposed method has been validated by determining these low molecular weight aminothiols in human urine and plasma samples with satisfactory results, and thus it will have great potential application in clinical diagnosis. (C) 2011 Elsevier B.V. All rights reserved.
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