Article
Biochemical Research Methods
Saida Ibragic, Stefano Barbini, Josua Timotheus Oberlerchner, Antje Potthast, Thomas Rosenau, Stefan Bohmdorfer
Summary: This study investigated the antioxidative properties and chemical composition of Ephedra herb extracts using a variety of analytical methods, including HPTLC and GC-MS. The results provide insights into the qualitative analysis and chemical fingerprinting of different Ephedra species of various geographical origins.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Review
Chemistry, Analytical
Sanka N. Atapattu, Azamat Temerdashev
Summary: Gas chromatography is a widely used analytical technique, but not all analytes can be directly analyzed. Analytical derivatization, such as injector port derivatization, is often required to improve the suitability of analytes for gas chromatographic analysis. This technique is easy to use, efficient, and requires fewer hazardous chemicals.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Rongfan Ren, Minghui Yuan, Hongli Li, David Da Yong Chen
Summary: Carbohydrates are essential for energy supply and biological functions, but identifying and distinguishing their isomers has been challenging due to their similar structures and possible glycosidic linkages. This study developed an ionization tandem mass spectrometry method to characterize disaccharide isomers by in situ methylation. The method allowed for immediate methylation of hydroxyl groups on disaccharides, enabling their ionization and distinction through mass spectrometry. This approach was used to directly identify disaccharide isomers in real commercial products without complex pretreatment or chromatographic separation.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Iwona Wenio, Iwona Bartosiewicz, Dorota Derewiaka, Katarzyna Dewiszek, Kamila Karnilowicz
Summary: This paper proposes a simple and fast GC-MS/MS analysis method for the detection of ethylene oxide (EtO) bactericides in food. The method involves the derivatization of ethylene oxide to 2-chloroethanol, with minimized extraction steps and volume of extraction solvent, and purification of the final extract through a syringe filter. The method is in compliance with the latest European guidelines for pesticide residue analysis.
APPLIED SCIENCES-BASEL
(2023)
Article
Food Science & Technology
Sandra Pradana-Lopez, Ana M. Perez-Calabuig, John C. Cancilla, Laura Otero, Jose S. Torrecilla
Summary: This work presents a method to detect and quantify melamine in three different powdered milks by training convolutional neural networks with a photograph database. The optimized model achieved an overall accuracy of 98.7% during the validation phase and a 3.0% misclassification rate during blind testing, demonstrating its effectiveness as a quality and safety control method for the milk industry.
Article
Biochemical Research Methods
Alexander Bollenbach, Dimitrios Tsikas
Summary: This study reports a novel two-step derivatization procedure for the specific quantitative measurement of GSH by GC-MS, demonstrating the linearity of the method and minimal interference from other substances.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Biochemical Research Methods
Yaqiong Qin, Bing Wang, Shaofeng Liu, Lining Pan, Mantang Chen, Huapeng Cui, Ruihong Liu, Yunzhen Jia, Junlan Cai, Kejian Liu, Xiaoyu Wang, Fuwei Xie
Summary: This study developed a highly robust, sensitive, and precise method for the simultaneous analysis of 171 flavour additives in cigarette smoke. The method utilized silylation and GCMS/MS with an improved backflushing system, providing reliable results for regulating tobacco flavour additives.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Food Science & Technology
Sebastian Imperiale, Elke Kaneppele, Ksenia Morozova, Federico Fava, Demian Martini-Losch, Peter Robatscher, Giovanni Peratoner, Elena Venir, Daniela Eisenstecken, Matteo Scampicchio
Summary: Hay milk is a traditional dairy product protected as traditional specialty guaranteed (TSG), with strict regulations to ensure authenticity. The study proposes two analytical approaches using GC-MS and HPLC-HRMS to differentiate milk containing silage, successfully predicting the use of maize or grass silage in cow's diet with 100% recognition. The findings suggest that analytical approaches based on HRMS are a viable authentication method for hay milk.
Article
Plant Sciences
Dorsaf Ben Hassine, Rami Rahmani, Jean Pierre Souchard, Manef Abderrabba, Jalloul Bouajila
Summary: This study investigated the chemical composition and biological activities of the leaves of two Eucalyptus species. The methanolic extracts were found to be rich in phenolic compounds, and new molecules were identified in the extracts. All extracts exhibited antioxidant, enzyme inhibitory, and antiproliferative activities against cancer cells.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Biochemical Research Methods
Elisabet Foguet-Romero, Iris Samarra, Maria Guirro, Marc Riu, Jorge Joven, Javier A. Menendez, Nuria Canela, Antoni DelPino-Rius, Salvador Fernandez-Arroyo, Pol Herrero
Summary: Advances in metabolomics analysis and data treatment have increased our understanding of complex biological systems. Gas chromatography-mass spectrometry (GC-MS) is one of the most commonly used methods. However, method standardization and derivatization steps in GC-MS can introduce experimental errors and be time-consuming. This study proposes the injection-port derivatization (IPD) method to increase analysis throughput.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Shuyu Liu, Yayun Tang, Yang Lu, Yawen Guo, Kaizhou Xie, Fanxun Guan, Pengfei Gao, Yali Zhu, Yuhao Dong, Tao Zhang, Genxi Zhang, Guojun Dai, Xing Xie
Summary: A novel precolumn derivatization-GC-MS/MS method was developed and applied to determine decoquinate residues in chicken tissues. The method met the requirements for veterinary drug residue detection in China, the US, and the EU. Analysis of 60 chicken tissue samples showed that only one sample of chicken muscle contained 15.6 μg/kg decoquinate residue.
Article
Biochemical Research Methods
Nerea Lorenzo-Parodi, Erich Leitner, Torsten C. Schmidt
Summary: This study compares three gas chromatographic methods for analyzing ten iodinated derivatives of aromatic amines, and finds that all methods and most analytes have good detection limits and precision. Urine samples from smokers and non-smokers were successfully analyzed, and differences in concentration were observed.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Daniel Kuhn, Claudia Schlabitz, Maiara Giroldi, Daniel Neutzling Lehn, Lucelia Hoehne, Claucia Fernanda Volken de Souza
Summary: This study validated a method for quantifying free amino acids in cheese whey protein concentrate and hydrolysate using GC-MS. Trichloroacetic acid was found to enable the determination of 13 free amino acids. The method was validated in terms of recovery, limits of detection, and limits of quantification, and can be used in food laboratories for quantifying amino acids in solid dairy products.
INTERNATIONAL DAIRY JOURNAL
(2023)
Article
Food Science & Technology
Elena Bergamasco, Gregorio Peron, Andrea Venerando, Shakil Ahmed Polash, Ravi Shukla, Stefania Sut, Stefano Dall'Acqua, Antonio Masi
Summary: This study provides a comprehensive characterization of sulfur-containing metabolites in asparagus using LC-MS and GC-MS. 80 S-containing compounds were identified and their distribution in different parts of the spears was assessed. The identified compounds are considered to contribute to the taste and odor properties of green and white asparagus.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemical Research Methods
Ming-Jia Zhang, Jing Chou, Zhi-Wei Sun, Jin-Hui Zhao, Jing Guo, Jia-Ying Yu, Si-Qi Gao, Ying-Shu Tang, Li-Yan Liu
Summary: This study evaluated a protocol for detecting 26 organic acids in serum based on ultrasound-assisted derivatization using gas chromatography/mass spectrometry (GC/MS). Results showed satisfactory linearity, low detection limits, good reproducibility, acceptable accuracy, and stability, suggesting the efficacy of this protocol for clinical applications in evaluating metabolic changes related to organic acid profiles.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2021)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)