Article
Agriculture, Multidisciplinary
Svenja Schneider, Simon Hammann, Heiko Hayen
Summary: A comprehensive analysis of polar lipids in whole grain wheat and oat was conducted, resulting in the identification of 67 lipid species belonging to nine polar lipid classes. The relative composition of four specific lipid classes was investigated in detail.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Applied
Jie Wang, Jie Liao, Honghai Wang, Xiaofang Zhu, Linqiu Li, Weibo Lu, Gongshuai Song, Qing Shen
Summary: A method for analyzing plasmalogens in shellfish was developed, with mussel identified as having the highest content and being a good choice for large-scale preparation.
Review
Chemistry, Analytical
Qianying Sheng, Meiyan Liu, Minbo Lan, Guangyan Qing
Summary: Hydrophilic interaction liquid chromatography (HILIC) is an attractive complementary method to reversed phase LC for the separation of hydrophilic or strongly polar substances. The advancements in HILIC have significantly improved sensitivity, analyte coverage, throughput, analysis speed, and resolution in bio-separation and bio-analytical chemistry.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Xixi Jia, Xiaowei Li, Liang Zhao, Saiwa Liu, Zhinan Wang, Jingjing Du, Lirui Ge, Kunxia Zhao, Lu Tian, Jian Xu, Zuhao Yan, Jiangyi Ding, Xi Xia
Summary: A sensitive and selective method using hydrophilic interaction chromatography combined with tandem mass spectrometry was developed to detect 18 aminoglycosides in bovine milk. Aminoglycosides were extracted and isolated from milk using a mixture of trichloroacetic acid, ammonium acetate, ethylenediaminetetraacetic acid, and sodium chloride, and an Oasis HLB solid-phase extraction cartridge. The method showed satisfactory separation of the analytes with detection and quantification limits ranging from 0.05-2 μg/kg and 0.1-5 μg/kg, respectively. The method was successfully applied to detect neomycin in one fresh bovine milk sample, and it provides a powerful tool for the surveillance of aminoglycosides in bovine milk.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Chemistry, Analytical
Stefano Dugheri, Nicola Mucci, Donato Squillaci, Elisabetta Bucaletti, Giovanni Cappelli, Lucia Trevisani, Cecile Valsecchi, Viviana Consonni, Fabio Gosetti, Davide Ballabio, Giulio Arcangeli
Summary: Antineoplastic drugs, known for their severe toxic effects, require sensitive monitoring methods to address surface contaminations within preparation and administration units. An orthogonal normal phase analysis method has been developed to complement the limitations of the more common reversed-phase methods, and has shown promise in reliable relationship evaluation between chromatographic retention and chemical-structural features of the drugs under analysis.
Article
Biochemical Research Methods
Lili Guo, Shruti Nayak, Yuan Mao, Ning Li
Summary: Adding glycine to mobile phases during HILIC-ESI-MS analysis significantly enhances the sensitivity of mass spectrometry detection, increases ion intensities, improves tandem MS spectral quality of N- and O-glycans, and does not affect chromatographic performance.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Biochemical Research Methods
Xiaoqing Wang, Jian Cui, Jin Zhou, Shuo Wang, Yiming Gu, Xiaowei Liu, Shudong Wang
Summary: Vinyl modified silica was synthesized through a silane coupling reaction and a poly-acrylamide modified silica (PAM-SIL) stationary phase was prepared via free radical polymerization. The separation performance of the stationary phases could be regulated by controlling the polymer structure, showing potential for future separation and analysis applications.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Agriculture, Multidisciplinary
Andrea Castellaneta, Ilario Losito, Beniamino Leoni, Pietro Santamaria, Cosima Damiana Calvano, Tommaso R. Cataldi
Summary: Microgreens are a special type of vegetal product with potentially high content of various nutrients, including glycerophospholipids. The study found that glycerophospholipids in different microgreens are rich in essential fatty acids, which can serve as additional sources in vegetarian/vegan diets.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Nikolay Berezhnoy, Amaury Cazenave-Gassiot, Liang Gao, Juat Chin Foo, Shanshan Ji, Viduthalai Rasheedkhan Regina, Pui Khee Peggy Yap, Markus R. Wenk, Staffan Kjelleberg, Thomas William Seviour, Jamie Hinks
Summary: This study investigates the changes in lipid composition of Escherichia coli under different environmental conditions, particularly during growth phases and under stress. The researchers found that the cyclopropanation of fatty acyl chains plays a key role in lipid diversity, with a bias towards shorter chains. Additionally, the presence of ampicillin and chloramphenicol leads to an increase in saturated fatty acyl chains, affecting membrane properties and bacterial stress tolerance.
Article
Food Science & Technology
Cheng Li, Zhiqian Liu, Leah Marett, Jennie Pryce, Simone Rochfort
Summary: Milk is a rich source of lipids, and LC-MS is the predominant technique for lipid identification and quantification. However, no standard methods are currently available for the quantification of milk phospholipids. This study aims to examine the factors influencing the quantification outcome of bovine milk phospholipids and discuss the pros and cons of current LC-MS methods.
Article
Biochemical Research Methods
Bingchuan Wei, Jenny Wang, Lulu Dai, Bifan Chen, Kelly Zhang
Summary: This study developed a method for analyzing sgRNA and demonstrated the potential of HILIC-MS for analyzing large oligonucleotides.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Isabelle Kohler, Michel Verhoeven, Rob Haselberg, Andrea F. G. Gargano
Summary: Proteomics and metabolomics have become increasingly important in clinical applications, with RPLC-MS and HILIC-MS techniques playing crucial roles in biomarker discovery and disease diagnosis. While RPLC-MS and HILIC-MS complement each other in resolving the complexity of proteome and metabolites, they also have technical limitations and potential solutions that need to be addressed.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Michaela Chocholouskova, Denise Wolrab, Robert Jirasko, Hana Studentova, Bohuslav Melichar, Michal Holcapek
Summary: This study systematically investigates the effects of different lipid class separation methods and mass spectrometers on quantitative lipidomic data, finding slight variations in molar concentrations even within the same laboratory using different platforms. Normalization to a reference sample can significantly reduce these differences, leading to highly homogenous molar concentrations of individual lipid species. This strategy suggests a potential approach for reporting comparable quantitative results regardless of the analytical platform and mass spectrometer used.
Article
Chemistry, Analytical
Mengyao Fu, Baichun Wang, Linhua Yi, Xueting Jin, Yinghua Yan, Chuan-Fan Ding
Summary: A novel porous bifunctionalized composite material with superhydrophilicity and small steric hindrance was synthesized via a simple method, showing excellent performance in glycopeptides enrichment.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Biochemical Research Methods
Dana Moravcova, Richard Cmelik, Jana Krenkova
Summary: This study demonstrates the advantages of using mixtures of organic solvents for the separation of labeled oligosaccharides under hydrophilic interaction liquid chromatography conditions. Successful separation of labeled isomeric trisaccharides was achieved, and adjusting the volume ratio of solvents allowed for rapid separation of different labeled saccharides.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biophysics
Klara R. Mertinkus, J. Tassilo Grun, Nadide Altincekic, Jasleen Kaur Bains, Betul Ceylan, Jan-Peter Ferner, Lucio Frydman, Boris Fuertig, Martin Hengesbach, Katharina F. Hohmann, Daniel Hymon, Jihyun Kim, Bozana Knezic, Mihajlo Novakovic, Andreas Oxenfarth, Stephen A. Peter, Nusrat S. Qureshi, Christian Richter, Tali Scherf, Andreas Schlundt, Robbin Schnieders, Harald Schwalbe, Elke Stirnal, Alexey Sudakov, Jennifer Voegele, Anna Wacker, Julia E. Weigand, Julia Wirmer-Bartoschek, Maria A. Wirtz Martin, Jens Woehnert
Summary: The ongoing pandemic of COVID-19 is caused by the SARS-CoV-2 virus, which belongs to the Betacoronavirus genus. The genome of SCoV2 contains conserved RNA elements that are involved in viral replication and translation. Studying the structure of these elements can support the development of new therapeutics against COVID-19.
BIOMOLECULAR NMR ASSIGNMENTS
(2022)
Review
Biochemistry & Molecular Biology
J. Tassilo Grun, Harald Schwalbe
Summary: G-quadruplexes (G4), found in various locations within the human genome, play essential roles in cell regulation processes. Understanding their intricate structures, polymorphism, and folding mechanisms is crucial for unraveling their biological functions and potential therapeutic applications.
Article
Biochemistry & Molecular Biology
Bozana Knezic, Sara Keyhani-Goldau, Harald Schwalbe
Summary: The development of enzymatic function in an RNA world is closely related to specific RNA folds. By investigating the conversion of ribozymes in a neutral network, the relationship between ribozyme function and secondary structure can be understood.
Article
Biochemistry & Molecular Biology
Tatjana Schamber, Oliver Binas, Andreas Schlundt, Anna Wacker, Harald Schwalbe
Summary: Riboswitches are regulatory RNA elements that undergo conformational changes upon binding specific ligands. The guanidine-II riboswitch investigated here binds guanidinium and forms a kissing loop-loop interaction. Structural changes were studied using NMR spectroscopy, confirming the observed structure and characterizing the interaction with Mg2+ and ligand. Mutant constructs were also investigated to understand functional differences between the two hairpins P1 and P2. Formation of oligomeric states of the riboswitch induced by Mg2+ and ligand binding were observed through small-angle X-ray scattering (SAXS) and NMR DOSY data.
Article
Chemistry, Physical
Jihyun Kim, Mihajlo Novakovic, Sundaresan Jayanthi, Adonis Lupulescu, Eriks Kupce, J. Tassilo Gruenorcid, Klara Mertinkus, Andreas Oxenfarth, Harald Schwalbe, Lucio Frydman
Summary: Hadamard encoded saturation transfer can improve the efficiency and sensitivity of NOE-based NMR correlations in proteins, glycans, and RNAs. However, it fails in correlating labile protons within a pool. This study proposes an extended Hadamard scheme that allows for artifact-free correlations within the labile pool and between labile and non-labile protons, while still benefiting from Hadamard encoding and solvent repolarizations. The extended Hadamard scheme demonstrates clean, artifact-free, sensitivity-enhancing performance on RNA fragments derived from the SARS-CoV-2 genome.
Article
Biochemical Research Methods
Timothy Warwick, Sandra Seredinski, Nina M. Krause, Jasleen Kaur Bains, Lara Althaus, James A. Oo, Alessandro Bonetti, Anne Dueck, Stefan Engelhardt, Harald Schwalbe, Matthias S. Leisegang, Marcel H. Schulz, Ralf P. Brandes
Summary: This paper presents a new method for predicting RNA-DNA triple helix formation and develops the TriplexAligner program for predicting such interactions. The method predicts RNA-DNA interactions more accurately than previous methods in humans and mice and has been validated through biophysical experiments. This work is important for better understanding triplex formation and genome-wide analysis of RNA-DNA interactions.
BRIEFINGS IN BIOINFORMATICS
(2022)
Article
Multidisciplinary Sciences
Matthias S. Leisegang, Jasleen Kaur Bains, Sandra Seredinski, James A. Oo, Nina M. Krause, Chao-Chung Kuo, Stefan Guenther, Nevcin Senturk Cetin, Timothy Warwick, Can Cao, Frederike Boos, Judit Izquierdo Ponce, Shaza Haydar, Rebecca Bednarz, Chanil Valasarajan, Dominik C. Fuhrmann, Jens Preussner, Mario Looso, Soni S. Pullamsetti, Marcel H. Schulz, Hendrik R. A. Jonker, Christian Richter, Flavia Rezende, Ralf Gilsbach, Beatrice Pflueger-Mueller, Ilka Wittig, Ingrid Grummt, Teodora Ribarska, Ivan G. Costa, Harald Schwalbe, Ralf P. Brandes
Summary: Using bioinformatic techniques and biophysical studies, we identified a functionally important DNA:DNA:RNA triplex-forming lncRNA HIF1 alpha-AS1 in human endothelial cells, which reduces the expression of multiple genes by inducing triplex formation as an adapter. Additionally, we found that HIF1 alpha-AS1 is down-regulated in pulmonary hypertension and loss-of-function enhances gene de-repression and angiogenic capacity.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Marie-Theres Hutchison, Giovanni Bellomo, Alexey Cherepanov, Elke Stirnal, Boris Fuertig, Christian Richter, Verena Linhard, Elina Gurewitsch, Moreno Lelli, Nina Morgner, Thomas Schrader, Harald Schwalbe
Summary: This study investigates the aggregation process of A beta 42 protein, which is directly related to the pathogenesis of Alzheimer's disease. Real-time solution NMR spectroscopy is used to track the A beta 42 aggregation kinetics and analyze the impact of low-molecular-weight inhibitors. The findings reveal the differential effects of low-molecular-weight inhibitors on early-stage aggregation.
Article
Chemistry, Multidisciplinary
Alexey Sudakov, Bozana Knezic, Martin Hengesbach, Boris Fuertig, Elke Stirnal, Harald Schwalbe
Summary: More than 170 post-transcriptional modifications of RNAs have been identified. However, the detailed investigation of these modifications is limited due to the difficulty in producing large RNAs containing these modifications. This study reports the chemo-enzymatic synthesis of RNA modifications and fluorine-modified analogs, allowing the synthesis of large RNAs. The incorporation of labeled nucleotides in the riboswitch demonstrated the applicability of the modified large RNAs in characterizing RNA fold and dynamics.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Stefan Kunert, Verena Linhard, Sara Weirich, Michel Choudalakis, Florian Osswald, Lisa Kraemer, Anja R. Koehler, Alexander Broehm, Jan Wollenhaupt, Harald Schwalbe, Albert Jeltsch
Summary: The highly expressed DNMT3A DNA methyltransferase and MECP2 methylation reader in neurons interact through their DNMT3A-ADD and MECP2-TRD domains, and the interaction between MECP2 regulates the activity and subnuclear localization of DNMT3A. The interface of these two domains was mapped, revealing that the ADD domain interacts with the TRD domain through the D529-D531 region, while the TRD domain interacts with the ADD domain through residues 214-228, including K219 and K223. The mutations at the ADD-TRD interface disrupt the cellular interaction of these proteins in NIH3T3 cells.
Editorial Material
Chemistry, Multidisciplinary
Elke Duchardt-Ferner, Jan Ferner, Boris Fuertig, Martin Hengesbach, Christian Richter, Andreas Schlundt, Sridhar Sreeramulu, Anna Wacker, Julia E. Weigand, Julia Wirmer-Bartoschek, Harald Schwalbe
Summary: The COVID-19 outbreak in December 2019 led to the establishment of international collaborations to understand and combat the virus. The NMR community has come together to study the genome and proteome of SARS-CoV-2 and test their interaction with low-molecular-weight binders. Due to the global pandemic and extended lockdowns, research has shifted from local collaborations to digital formats, allowing immediate discussion of unpublished results, data analysis, sample sharing, and coordinated research between more than 50 groups from 18 countries. Key lessons and challenges for post-pandemic research are discussed.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
J. Tassilo Grun, Jihyun Kim, Sundaresan Jayanthi, Adonis Lupulescu, Eriks Kupce, Harald Schwalbe, Lucio Frydman
Summary: Magnetization transfer experiments can provide site-specific information in nuclear magnetic resonance (NMR). Repeated saturation magnetization transfer (SMT) experiments can enhance connectivities revealed via the nuclear Overhauser effect (NOE), but may also lead to artifacts such as spill-over effects and NOE oversaturation. Another potential artifact arises when labile protons of interest are bound to 15N-labeled heteronuclei. These phenomena are experimentally demonstrated in this article, along with proposed solutions to overcome them.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Tamer Ali, Sandra Rogala, Nina M. Krause, Jasleen Kaur Bains, Maria-Theodora Melissari, Sandra Waehrisch, Harald Schwalbe, Bernhard G. Herrmann, Phillip Grote
Summary: Long non-coding RNAs can regulate cell function and other genes on the transcriptional level through mechanisms such as direct interaction with DNA and formation of RNA:dsDNA triplexes. In this study, we genetically deleted the triplex forming sequence from the lncRNA Fendrr in mice and found its partial requirement for Fendrr function in vivo. Loss of the triplex forming site in developing lungs led to dysregulation of gene programs associated with lung fibrosis, with some genes containing a triplex site directly at their promoter. We confirmed the formation of an RNA:dsDNA triplex with target promoters in vitro and implicated the synergistic role of Fendrr and the Wnt signaling pathway in lung fibrosis.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Chemistry, Analytical
Robert Tannenberg, Martin Paul, Bettina Roeder, Santosh L. Gande, Sridhar Sreeramulu, Krishna Saxena, Christian Richter, Harald Schwalbe, Claudia Swart, Michael G. Weller
Summary: Cardiac vascular diseases, especially acute myocardial infarction (AMI), are a major cause of death worldwide. In this study, a chemiluminescence-based immunosensor was developed for the rapid and accurate measurement of cardiac troponin I (cTnI), a specific biomarker for AMI diagnosis. The sensor achieved selective and quasi-online cTnI determination within ten minutes using a flow cell and a sensitive CMOS camera for optical readout. The sensitivity and selectivity of the sensor were tested with recombinant proteins and diluted blood plasma and serum, demonstrating its potential for clinical applications.
Article
Chemistry, Multidisciplinary
Alexey V. Cherepanov, Harald Schwalbe
Summary: The quest for atomic structures of microsecond reaction intermediates is a cutting-edge research in the field of biochemistry. However, there is currently a lack of experimental methods for preparing time-resolved samples. In this study, a single-turnover technique for nanosecond initiation and suspension of biomolecular reactions in the microsecond time domain is developed. The reactions can be started by temperature jump or direct mixing and stopped by hyperquenching in liquid cryogen at 77K. The diverse morphology of nanoscale glassy bodies, including thin sheets suitable for structure analysis by transmission electron cryomicroscopy, is observed. Furthermore, a novel method of concentrating reaction intermediates for structural studies using low-temperature techniques is presented.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)