Article
Biochemical Research Methods
Johannes Winderl, Eric Neumann, Jurgen Hubbuch
Summary: Fiber-based CEX adsorbents show comparable performance to resins in removing mAb aggregates, with higher productivity and lower buffer consumption observed in controlled grafted fibers. The use of such adsorbents for mAb aggregate removal is feasible with performance on par with existing resin materials.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Thuy Tran, Erik Martinsson, Sergio Vargas, Ingemar Lundstrom, Carl-Fredrik Mandenius, Daniel Aili
Summary: The production of therapeutic monoclonal antibodies is a complex process that requires extensive analytical characterization. Aggregation of antibodies is common and problematic, as it can affect the product quality and immunogenicity. Rapid detection of aggregates is challenging with current analytical techniques.
ANALYTICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Young Hoon Oh, Matthew L. Becker, Kerri M. Mendola, Leila H. Choe, Lie Min, Kelvin H. H. Lee, Yinges Yigzaw, Alexander Seay, Jerome Bill, Xuanwen Li, David J. Roush, Steven M. Cramer, Stefano Menegatti, Abraham M. Lenhoff
Summary: In the production of biopharmaceuticals, the presence of residual host-cell proteins (HCPs) is a critical quality concern. Measurements on seven monoclonal antibody (mAb) processes revealed that HCP aggregates, not necessarily derived from chromatin, are important factors in the persistence of HCPs. These aggregates can also interfere with accurate detection of HCPs using proteomics methods. The study also highlights the difficulty in removing certain HCPs due to their functional complementarity to the biopharmaceutical product.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biochemical Research Methods
Urh Cernigoj, Blaz Nemec, Ales Strancar
Summary: The rise of biosimilar monoclonal antibodies has sparked renewed interest in the composition and separation of monoclonal antibody (mAb) charge variants. Sample displacement chromatography (SDC) has shown potential in enhancing efficiency and productivity in separating mAb charge variants, especially when used in combination with weak cation exchange chromatographic columns. The efficiency of the SDC process is flow rate independent, and at optimal conditions, it can achieve a productivity that is three times higher than the standard bind-elute separation method.
Article
Engineering, Chemical
Sobhana A. Sripada, Driss Elhanafi, Leonard B. Collins, Taufika I. Williams, Marina Y. Linova, John M. Woodley, Cristiana Boi, Stefano Menegatti
Summary: This study presents the development and validation of a novel adsorbent, PichiaGuard, which is functionalized with peptide ligands and targets the whole spectrum of K. phaffii HCPs for protein purification. The PichiaGuard adsorbent has high HCP binding capacity and successfully purified monoclonal antibodies and fragments, showing its potential for modern biopharmaceutical processing.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Roxana Roshankhah, Guoqiang Chen, Yating Xu, Nikhila Butani, Yves Durocher, Robert Pelton, Raja Ghosh
Summary: This study examines the purification of Trastuzumab using cation-exchange z(2) laterally-fed membrane chromatography (z(2)LFMC). The results show that the z(2)LFMC process is comparable to protein A affinity chromatography in terms of purity and recovery, but it is faster and does not require acidic conditions. The productivity of Trastuzumab with the z(2)LFMC process is more than 3 times higher than with column-based purification. Therefore, z(2)LFMC could be a cheaper and more efficient alternative to protein A affinity chromatography.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Ryan R. Davis, Fletcher Suber, Ian Heller, Boxuan Yang, Juan Martinez
Summary: Improving productivity and decreasing costs for biotherapeutic agents is a key driver in the biologics manufacturing industry. Advances in upstream processes have outpaced downstream operations, particularly in monoclonal antibody purification. Utilizing cellulose nanofibers with a protein A ligand in continuous chromatography can greatly increase productivity and reduce costs.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Microbiology
Haiqiao Bian, Chong Yu, Yanwu Wei, Li Feng, Changming Liu, Liping Huang
Summary: The study successfully purified PCV2 virus strains using affinity chromatography, resulting in high purity and no significant changes in antigenic characteristics after purification. It was found that PCV2 viruses can be successfully purified, but the yield and purity may vary among different subtypes, and the purified virus can be used for virus structure analysis and viral adsorption and invasion research.
Article
Biophysics
Atsushi Hirano, Shogo Kanoh, Kentaro Shiraki, Momoyo Wada, Masahiro Kitamura, Katsuya Kato
Summary: This study discovered that phosphate-modified zirconia nanoparticles can selectively adsorb immunoglobulin G (IgG) antibodies with high capacity while maintaining their molecular conformation. Compared to traditional Protein A ligands, zirconia nanoparticles have higher affinity for human IgG antibodies, especially IgG3. Due to their chemical and mechanical stability, zirconia nanoparticles can be used in batch methods and chromatography as an alternative to Protein A chromatography for the purification of antibody drugs.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Biochemical Research Methods
Gunasekaran Dhandapani, Ellen Wachtel, Mordechai Sheves, Guy Patchornik
Summary: The study demonstrates that a non-chromatographic, ligand-free approach using detergent aggregates can effectively purify antibodies, with varying efficacy observed for different types of detergents. Furthermore, the purification method allows for continuous purification mode through filtration, achieving high recovery rates and preservation of Ab specificity.
Article
Biochemical Research Methods
Xin Li, Wanhui Liu, Heshui Li, Xin Wang, Yanyan Zhao
Summary: A new method for capturing and purifying untagged nanobodies (Nbs) using mixed weak cation exchange chromatography and cation exchange chromatography was successfully established in this study. The optimal loading condition for capturing anti-NGF Nb using Capto MMC was found to be pH 4.0-5.0 through experiments. This method showed high purity and yield of products at both laboratory scale and commercial production scale.
PROTEIN EXPRESSION AND PURIFICATION
(2022)
Article
Engineering, Chemical
Joshua Peles, Fatemeh Fallahianbijan, Benjamin Cacace, Christina Carbrello, Sal Giglia, Andrew L. Zydney
Summary: This study investigates the effect of operating pressure on membrane fouling and filter capacity in virus removal filtration. The results show that increasing pressure reduces fouling and increases filter capacity. Furthermore, pressure has an impact on both pore blockage and cake resistance.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Multidisciplinary Sciences
Ozge Tatli, Yagmur Oz, Baran Dingiloglu, Duygu Yalcinkaya, Ezgi Basturk, Melis Korkmaz, Latif Akbulut, Derya Hatipoglu, Cansin Kirmacoglu, Buse Akgun, Kubra Turk, Orkun Pinar, Berna Sariyar Akbulut, Zeynep Atabay, Eda Tahir Turanli, Dilek Kazan, Gizem Dinler Doganay
Summary: Antibody fragments have advantages over antibodies in certain therapeutic settings, but their purification is more challenging due to their greater chemical diversity. This study developed a new purification platform for antibody fragments, which successfully cleared Fab and process-related impurities with a high overall process recovery.
Article
Chemistry, Multidisciplinary
Thomas Powell, Michael J. Knight, Amanda Wood, John O'Hara, William Burkitt
Summary: This study characterized inter-protein isopeptide bonds in mAb aggregates for the first time, showing diversity in clipping of the antibody's CH2 domain and identifying 14 isopeptide bonds. The results revealed the presence of intermolecular isopeptide bonds between the side chains of acidic amino acids (aspartate and glutamate) and lysine residues in mAb aggregates, providing insights into a chemical mechanism for spontaneous isopeptide bond formation.
PHARMACEUTICAL RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Jonas Schilz, Charlotte Clement, Franziska Greiner, Arne Skerra
Summary: A novel strategy for the efficient and gentle affinity purification of PASylated proteins is reported, which allows direct one-step purification from cell extracts or culture supernatants while avoiding harsh elution conditions. This method can improve the efficiency of biological drug development.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Immunology
Andrew E. Nixon, Jie Chen, Daniel J. Sexton, Arumugam Muruganandam, Alan J. Bitonti, Jennifer Dumont, Malini Viswanathan, Diana Martik, Dina Wassaf, Adam Mezo, Clive R. Wood, Joseph C. Biedenkapp, Chris TenHoor
FRONTIERS IN IMMUNOLOGY
(2015)
Article
Rheumatology
Kazuyo Kaneko, Richard O. Williams, Daniel T. Dransfield, Andrew E. Nixon, Ann Sandison, Yoshifumi Itoh
ARTHRITIS & RHEUMATOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Jon A. Kenniston, Brandy M. Taylor, Gregory P. Conley, Janja Cosic, Kris J. Kopacz, Allison P. Lindberg, Stephen R. Comeau, Kateri Atkins, Jameson Bullen, Christopher TenHoor, Burt A. Adelman, Daniel J. Sexton, Thomas E. Edwards, Andrew E. Nixon
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Biochemical Research Methods
Michael Dileo, Arthur Ley, Andrew E. Nixon, Jie Chen
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2017)
Article
Biochemistry & Molecular Biology
Danlin Yang, John J. Correia, Walter F. Stafford, Christopher J. Roberts, Sanjaya Singh, David Hayes, Rachel Kroe-Barrett, Andrew Nixon, Thomas M. Laue
Article
Biochemical Research Methods
Jon A. Kenniston, Gregory P. Conley, Daniel J. Sexton, Andrew E. Nixon
ANALYTICAL BIOCHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
Daniel J. Sexton, Ting Chen, Diana Martik, Petr Kuzmic, Guannan Kuang, Jie Chen, Andrew E. Nixon, Bruce L. Zuraw, Rosanna M. Forteza, William M. Abraham, Clive R. Wood
BIOCHEMICAL JOURNAL
(2009)
Article
Biotechnology & Applied Microbiology
Greg P. Conley, Malini Viswanathan, Ying Hou, Doug L. Rank, Allison P. Lindberg, Steve M. Cramer, Robert C. Ladner, Andrew E. Nixon, Jie Chen
BIOTECHNOLOGY AND BIOENGINEERING
(2011)
Article
Oncology
Laetitia Devy, Lili Huang, Laurent Naa, Niranjan Yanamandra, Henk Pieters, Nicolas Frans, Edward Chang, Qingfeng Tao, Marc Vanhove, Annabelle Lejeune, Reinoud van Gool, Daniel J. Sexton, Guannan Kuang, Douglas Rank, Shannon Hogan, Csaba Pazmany, Yu Lu Ma, Sonia Schoonbroodt, Andrew E. Nixon, Robert C. Ladner, Rene Hoet, Paula Henderikx, Chris TenHoor, Shafaat A. Rabbani, Maria Luisa Valentino, Clive R. Wood, Daniel T. Dransfield
Article
Biochemistry & Molecular Biology
Jon A. Kenniston, Ryan R. Faucette, Diana Martik, Stephen R. Comeau, Allison P. Lindberg, Kris J. Kopacz, Gregory P. Conley, Jie Chen, Malini Viswanathan, Niksa Kastrapeli, Janja Cosic, Shauna Mason, Mike DiLeo, Jan Abendroth, Petr Kuzmic, Robert C. Ladner, Thomas E. Edwards, Christopher TenHoor, Burt A. Adelman, Andrew E. Nixon, Daniel J. Sexton
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Review
Medicine, Research & Experimental
Andrew E. Nixon, Daniel J. Sexton, Robert C. Ladner
Article
Oncology
Susanne Hipp, Vladimir Voynov, Barbara Drobits-Handl, Craig Giragossian, Francesca Trapani, Andrew E. Nixon, Justin M. Scheer, Paul J. Adam
CLINICAL CANCER RESEARCH
(2020)
Article
Multidisciplinary Sciences
Lucky Ahmed, Priyanka Gupta, Kyle P. Martin, Justin M. Scheer, Andrew E. Nixon, Sandeep Kumar
Summary: By analyzing the physicochemical descriptors of Fv regions in marketed antibody-based biotherapeutics, this study derived a set of descriptors that can help guide hit selection, lead identification, and optimization of biotherapeutic candidates. The research found that 33% of CST antibodies deviate in physicochemical properties from currently marketed biotherapeutics, providing valuable information for risk assessment and collaborations.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Medicinal
Helen Haixia Wu, Maureen Crames, Yangjie Wei, Dongmei Liu, Kristina Gueneva-Boucheva, Ikbae Son, Lee Frego, Fei Han, Rachel Kroe-Barrett, Andrew Nixon, Michael Marlow
Summary: Monoclonal antibodies, especially IgGs and Ig-based molecules, are widely used biotherapeutic drugs. The engineering of the Fc domain of these drugs to optimize their interactions with Fc gamma receptors (FcgRs) has been extensively studied in recent years. However, limited information is available regarding the effects of mutations and isotype differences on the physicochemical properties and manufacturability of these drugs. This study systematically characterizes the effects of mutations and IgG4 isotype on the stability and manufacturability of six Fc variants, providing a basis for selecting appropriate Fc regions and predicting the impact of mutations on the drug development process.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)