Article
Chemistry, Analytical
Jing Li, Danye Qiu, Feng Li, Jingwu Kang
Summary: A method was reported for preparing a poly(N-vinylpyrrolidone-co-pentaerythritol triacrylate copolymerization)-based monolithic capillary column for the separation of polar small molecular weight compounds with nano-liquid chromatography in hydrophilic interaction chromatography mode. The monolithic columns were prepared by copolymerization of N-vinylpyrrolidone and pentaerythritol triacrylate in a binary porogenic agent consisting of methanol and water. The polymerization solution was optimized for column permeability, theoretical plate number, asymmetric factor, and retention factor. The N-vinylpyrrolidone-based hydrophilic interaction chromatography stationary phase showed satisfactory separation selectivity for polar test analytes.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Qi Wu, Xiudan Hou, Xiangfei Zhang, Hui Li, Liang Zhao, Haitao Lv
Summary: Amphipathic CQDs modified silica stationary phase shows typical characteristics of RPLC/HILIC, achieving satisfactory separation of both hydrophobic and hydrophilic compounds. The retention mechanism is investigated, revealing main interactions such as hydrophobic interaction, pi-pi stacking, hydrogen-bonding and electrostatic interactions under RPLC mode. This work provides a new approach for synthesis of amphipathic CQDs with great prospects in separation science.
Article
Biochemical Research Methods
Houmei Liu, Pian Jin, Mi Jiang, Yu Duan, Guozheng Zhu, Hui Yu, Hongdeng Qiu
Summary: In this work, five amine-ligands were functionalized on mesoporous micro-silicas as stationary phases for HILIC. Among them, di-amine and tri-amine functionalized stationary phases showed outstanding separation and retention abilities, providing potential for exploring novel HILIC stationary phases.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Multidisciplinary
Jiao-Jiao Li, Zong-Jie Guan, Shang-Fu Yuan, Feng Hu, Quan-Ming Wang
Summary: The article describes the synthesis and isolation of alkynyl/chloride-protected gold nanoclusters, and presents the structures of three different clusters, including the new clusters Au-67 and Au-106 with complex surface structures and unique electrochemical properties.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Analytical
Sepideh Khaki Firooz, M. Farooq Wahab, Jeongjae Yu, Daniel W. Armstrong
Summary: Packed column SFC is popular for separations due to low CO2 cost and environmental benefits. New functionalized cyclofructan columns show improved selectivity and efficiency in achiral SFC separations. Adding small amounts of water to the methanol modifier in CO2 improves peak symmetries for amines. These new stationary phases offer greener approaches to separation problems.
Article
Polymer Science
Mahmut Erzengin, Gozde Baydemir Pesint, Okan Zenger, Mehmet Odabasi
Summary: A novel hydrophobic adsorbent was synthesized for the removal of lysozyme from aqueous solution, showing good adsorption performance and reusability after multiple cycles.
Review
Chemistry, Analytical
Hooi Tee Khoo, Chiuan Herng Leow
Summary: The article discusses the development of silica monolithic columns as an alternative approach to address the challenges of high back-pressure in HPLC systems. By utilizing smaller particle sizes and internal diameters, these columns have been widely investigated for fast and efficient separation of various molecules. The fabrication processes, derivatization, and endcapping techniques of the monolith are elaborated, along with highlighting the performance of such columns in key applications across different fields with various types of matrices.
Article
Pharmacology & Pharmacy
Yikun Liu, Ning He, Yingfang Lu, Weiqiang Li, Xin He, Zhentao Li, Zilin Chen
Summary: In this study, a styrene-based polymer monolithic column poly(VBS-co-TAT-co-AHM) with reversed-phase/hydrophilic interaction liquid chromatography (RPLC/HILIC) bifunctional separation mode was successfully prepared for capillary electrochromatography. The incorporation of VBS functional monomer enabled the monolith to achieve efficient separation of various compounds in RPLC or HILIC mode. The prepared poly(VBS-co-TAT-co-AHM) monolith exhibited excellent reproducibility and stability, making it highly valuable and promising for capillary electrochromatography (CEC).
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Kaixing Luo, Yu Luo, Yanjuan Liu, Yuefei Zhang, Wei Chen, Zhengwu Bai, Sheng Tang
Summary: In this work, a new strategy was adopted to simultaneously graft two different functional ligands onto the silica surface, resulting in a carbonyldiimidazolium/dodecyl dual-functionalized silica stationary phase (Sil-CDI-DD) using recycled commercial C18 columns as raw materials. The obtained Sil-CDI-DD exhibited enhanced separation selectivity and efficiency, providing multiple interaction sites for diverse target analytes and enabling the favorable resolution of hydrophilic nucleosides/nucleobases and weak polar environmental endocrine disruptors.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Mengyao Fu, Baichun Wang, Linhua Yi, Xueting Jin, Yinghua Yan, Chuan-Fan Ding
Summary: A novel porous bifunctionalized composite material with superhydrophilicity and small steric hindrance was synthesized via a simple method, showing excellent performance in glycopeptides enrichment.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Biochemical Research Methods
Takeshi Hara, Gino V. Baron, Kosuke Hata, Yoshihiro Izumi, Takeshi Bamba, Gert Desmet
Summary: The study examined the possibility of enhancing the phase ratio and retention factor in silica monoliths by applying a stationary phase layer via radical polymerization with octadecyl methacrylate (ODM). This new method showed improved retention factors and separation efficiencies compared to the traditional approach, demonstrating the potential for enhanced performance in chromatographic separations.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Zixing Xu, Yonglei Wu, Zhiqiang Deng, Jian Long, Nianrong Sun, Chunhui Deng
Summary: A novel magnetic mesoporous silica nanomaterial with strong hydrophilic property was developed for glycopeptide enrichment prior to MS analysis, showing remarkable size-exclusion effect and reusability. Combined with nano-LC-MS/MS, the glycopeptidome of serum from breast cancer patients was comprehensively analyzed, indicating great potential for glycopeptidomics research.
Article
Food Science & Technology
Qianchun Zhang, Yanqun Yang, Changbo Zhang, Yuguo Zheng, Yun Wu, Xingyi Wang
Summary: Patulin is a mycotoxin that can endanger food quality and human health, with its low concentration levels posing challenges in discrimination and quantification. Developing a highly specific sample preparation method is crucial for accurate analysis of patulin. The innovative approach presented in the study utilizes an aptamer-functionalized capillary monolithic polymer hybrid for the selective determination of patulin in food samples, showcasing sensitivity and selectivity for ultrasensitive detection of target molecules.
Article
Chemistry, Analytical
Ye Shu, Tong Chen, Tao Liu, Hechen Xu, Jinjin He, Zhihui Cai, Wanning Zhu, Zihan Zhao, Jun Jiang
Summary: In this study, spherical silica was modified with imidazole, amide, and sulfonic acid. The modified silica was used to create novel polymerization stationary phases for hydrophilic interaction liquid chromatography (HILIC). Various characterization techniques were employed to analyze the stationary phases, and their performance was evaluated using high performance liquid chromatography. The HILIC stationary phases exhibited excellent retention properties and selectivity compared to the conventional C18 column. They were successfully applied for the separation of sulfonamides, flavonoids, and Fructus Ligustri Lucidi extracts. Overall, the prepared surface polymeric stationary phases showed great potential for the analysis of polar components and complex samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemical Research Methods
Ziteng Yu, Zongying Li, Feifang Zhang, Bingcheng Yang
Summary: This study describes a novel polymer-based polar stationary phase for hydrophilic interaction chromatography (HILIC) with high hydrophilicity and good separation performance achieved by introducing multiple types of polar groups onto the microspheres.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)