Article
Engineering, Environmental
Evgeny E. Tereshatov, Jonathan D. Burns, Steven J. Schultz, Brooklyn D. Green, Gabriela A. Picayo, Laura A. McCann, Lauren A. McIntosh, Gabriel C. Tabacaru, Austin Abbott, Matthew Berko, Emily Engelthaler, Kris Hagel, Travis Hankins, Bryan Harvey, Lauren Hoekstra, Kylie Lofton, Sebastian Regener, Robert Rider, Maxwell Sorensen, Alexandra Tabacaru, David Thomas, Justin Tobar, Zachary Tobin, Sherry J. Yennello
Summary: A systematic study on astatine (At) sorption on extraction chromatography resins from nitric acid media has been conducted at Texas A&M University. Commercially available resins such as LN, TK400, SR, TRU, and UTEVA were included in this study. Understanding the behavior of At in nitric acid solutions is crucial for the development of a fast and reliable separation and purification system. The results showed high distribution coefficients for all the studied resins, with TRU and UTEVA resins reaching values as high as 400. The sorption uptake followed the order: TRU ≥ UTEVA > TK400 ≥ SR > LN. Thermodynamic models were developed for each resin to suggest possible mechanisms of At sorption, and sorption constants were estimated. Additionally, a mathematical model was proposed to describe bismuth (Bi) sorption by the studied resins in nitric acid media, and separation factors of At and Bi were estimated using the ratio of fit functions assigned to the same resin and acidity. Only the TRU resin was found to be unsuitable for efficient At isolation from Bi, while the other four resins showed promising results.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Polymer Science
Mahmut Erzengin, Gozde Baydemir Pesint, Okan Zenger, Mehmet Odabasi
Summary: A novel hydrophobic adsorbent was synthesized for the removal of lysozyme from aqueous solution, showing good adsorption performance and reusability after multiple cycles.
Article
Biochemical Research Methods
Weixian Deng, Jihui Sha, Kathrin Plath, James A. Wohlschlegel
Summary: In bottom-up proteomics, achieving deep proteome coverage requires peptide-level fractionation to simplify the complex peptide mixture. CIF is an effective offline separation strategy capable of increasing the depth of peptide analyte coverage.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Biochemical Research Methods
Joe Mahmoud, Matthew Higginson, Paul Thompson, Christopher Gilligan, Francis Livens, Scott L. Heath
Summary: Several novel extraction chromatography resins (EXC) have been synthesized by solvent impregnation method, and they show good performance in selectively extracting americium. Compared with previous complex synthesis methods, the solvent impregnation method reported in this study is simpler and more effective.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Multidisciplinary
Oliver Musl, Irina Sulaeva, Ivan Sumerskii, Arnulf Kai Mahler, Thomas Rosenau, Jana Falkenhagen, Antje Potthast
Summary: Lignosulfonates are industrial biorefinery products with significant variability and heterogeneity in their structural composition. This study utilizes a novel hydrophobic chromatography method to provide a comprehensive understanding of the characteristic composition of lignosulfonates and reveals significant differences in hydrophobic composition among different industrial lignosulfonates.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Chemistry, Analytical
Joe Mahmoud, Matthew Higginson, Christopher Gilligan, Paul Thompson, Francis Livens, Scott L. Heath
Summary: A method for rapid separation of americium from complex matrices using commercially available extraction chromatography resins is reported. The study demonstrates the effectiveness of these resins in purifying americium and lanthanides from various metal contaminants.
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Allyson Tucker Dilks, Joanne Gilchrist, Yik Lam, Nathan Nicholes, Brad Stanley
Summary: No-salt flowthrough hydrophobic interaction chromatography (HIC) is effective in removing impurities from bioprocess streams. This study demonstrates the operating principles of no-salt flowthrough HIC in antibody purification, showing robust aggregate clearance across various conditions. HMW reduction can be improved by adjusting protein load and/or concentration.
BIOTECHNOLOGY PROGRESS
(2023)
Article
Cell Biology
W. A. Gayan Chathuranga, Chamilani Nikapitiya, Jae-Hoon Kim, Kiramage Chathuranga, Asela Weerawardhana, Niranjan Dodantenna, Doo-Jin Kim, Haryoung Poo, Jae U. Jung, Chul-Ho Lee, Jong-Soo Lee
Summary: This study reveals that Gadd45(3) functions as a critical regulator in G3BP1-mediated stress granule formation and interferon signaling. It interacts with G3BP1, promoting the assembly of stress granules and activation of interferon signaling. Deficiency of Gadd45(3) impairs the immune response to RNA virus infection, leading to increased susceptibility in mice, and severely decreases the production of interferon and cytokines.
Article
Biochemical Research Methods
Miaoyuan Guo, Jianlong Ye, Chunyang Zheng, Jianqiang Meng
Summary: This study proposes a new modification method that simplifies the preparation process and improves the selectivity of the membrane for protein separation by combining mixed-mode chromatography and molecular imprinting technology. By utilizing Ugi four-component reaction and surface-initiated atom transfer radical polymerization, a dual-recognition membrane adsorber with enhanced performance was successfully prepared. The experimental results demonstrate that this membrane effectively separates the target protein while retaining its bioactivity.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Multidisciplinary
Jia Chen, Ning Yuan, Danni Jiang, Qian Lei, Bei Liu, Weiyang Tang, Kyung Ho Row, Hongdeng Qiu
Summary: A hydrophobic carbon dots (Glc-OCDs) grafted onto porous silica surface showed excellent separation performance in reversed-phase liquid chromatography, particularly in the separation of isomers, demonstrating promising potential for chromatographic separation applications.
CHINESE CHEMICAL LETTERS
(2021)
Article
Chemistry, Applied
Liang Guo, Yan-hua Li, Sheng Fang, Yang Pan, Jie Chen, Yue-cheng Meng
Summary: This article proposes a novel strategy for protein purification using epsilon-polylysine (EPL) through selective phase separation (SPS) under neutral pH conditions. The phase separation of EPL-protein systems is driven by electrostatic attraction (EA) and hydrophobic attraction (HA), and is a kinetic process following the nucleation and growth mechanism. Hydrophobic patches from proteins play a major role in the selectivity of phase separation.
FOOD HYDROCOLLOIDS
(2022)
Article
Chemistry, Multidisciplinary
Xinhao Liu, Abdol Hadi Mokarizadeh, Amal Narayanan, Prathamesh Mane, Avanti Pandit, Yen-Ming Tseng, Mesfin Tsige, Abraham Joy
Summary: Coacervation is a mechanism used by living cells to compartmentalize biomolecules, and synthetic coacervates can mimic the functions of biological coacervates. This study investigates the assembly and stabilization of nonionic coacervates through a library of polyesteramides, and shows that water-tertiary amide bridging hydrogen bonds and hydrophobic interactions play a role in their stability. The viscosity and interfacial tension of polyesteramide-based coacervates can be tuned by controlling temperature and the molar ratio between hydrophobic interaction sites and bridging hydrogen bonding sites. These nonionic coacervates can be engineered to form multiphasic structures similar to those found in living cells, and can be used to study cargo partitioning, biochemical reaction rates, and interorganellar signal transport.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Physical
Diana C. Belchior, Mara G. Freire
Summary: Egg white proteins, such as ovalbumin and lysozyme, were effectively separated using aqueous biphasic systems (ABS) and three phase partitioning (TPP) methods. By optimizing the ABS conditions, selective precipitation of lysozyme over ovalbumin was achieved, demonstrating the potential of ABS/TTP combined strategies for fractionating high-value proteins from complex matrices in a single-step.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Engineering, Chemical
Wei Shi, Shun-Nan Zhu, Hong Xu, Kai-Bin Li, Si-Qi Zhang, Tian-Yi Zhang, Chao-Ying Fang, Xiao-Bin Zhang, De-Man Han
Summary: This study explored the potential of dual-ligand hydrophobic charge induction chromatography for simultaneous recovery of bovine serum albumin and bovine immunoglobulin G. Results showed that dual-ligand mode had stable coupling efficiency ratio and broke through the adsorption capacity limit of single-ligand mode. It exhibited good adsorption performance for both components, confirming its effectiveness as a strategy for biological separation.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Materials Science, Multidisciplinary
Santeri Kurkinen, Sami Virolainen, Tuomo Sainio
Summary: The chromatographic separation of rare earth elements (REE) using the chelating aminopolycarboxylate ligand methylglycine N,N-diacetate (MGDA) as anionic complexes was experimentally studied. A synthetic mixture of La, Nd, and Eu was used to simulate a REE mixture obtained from processed secondary sources. Successful separation of the REEs was achieved using different strong base anion exchange resins, and the pKa values of the complexes were determined. Fractionation of a ternary La-Nd-Eu mixture was investigated, with La being recovered at approximately 80% purity and yield. Chromatographic separation was found to be an efficient process option for the recovery of pure REE from phosphogypsum.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)