Size-exclusion chromatography combined with small-angle X-ray scattering optics

Size-exclusion chromatography with on-line synchrotion radiation solution small-angle X-ray scattering optics, absorbance and/or refractive index detectors was evaluated by protein characterizations The radius of gyration Value and zero-angle scattering intensity of protein molecules eluted fiom the chromatography Column were estimated using this measurement system In addition, the characterization of the conformation of the eluted proteins was demonstrated for hen egg lysozyme and bovine Submaxillary mucin. The present technique will be useful for not only the determination of the radius of gyration value and molecular weight of proteins with dimensions of 1-10 nm, but also for the structural characterization of the macromolecules during the chromatography (C) 2009 Elseviei B.V. All rights reserved.