Review
Chemistry, Medicinal
Hussain Alessa, Amr L. Saber, Jalal T. Althakafy
Summary: This study aimed to summarize recent outcomes of chromatographic methods, such as gas chromatography and high-performance liquid chromatography, used for the determination of melatonin over the past three decades.
ARCHIV DER PHARMAZIE
(2022)
Review
Food Science & Technology
Huynh N. P. Dang, Joselito P. Quirino
Summary: Alkenylbenzenes are potentially toxic compounds found in food, especially in flavorings, requiring constant monitoring due to concerns about their health effects. The challenge in determining alkenylbenzenes in food lies in the complex sample matrices and low amounts present. Gas and liquid chromatography are commonly used separation techniques, with detection methods including flame ionisation and mass spectrometry.
Review
Chemistry, Analytical
Albert Perez-Alcaraz, Francesc Borrull, Marta Calull, Carme Aguilar
Summary: The consumption of synthetic cathinones has been increasing in recent years due to its affordability and accessibility as an alternative to amphetamines. Developing procedures capable of detecting and monitoring their consumption is crucial, and urine has been widely used for this purpose as it is non-invasive and provides larger sample volumes than other biological matrices. Chromatographic techniques are the most reported approach for determining cathinones in urine samples.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Apostolia Tsiasioti, Constantinos K. Zacharis, Paraskevas D. Tzanavaras
Summary: This article reviews recent studies on the determination of histamine in food matrices using derivatization-based separation methods. The derivatization of histamine plays an important role in enhancing sensitivity, selectivity, and compatibility to analytical techniques. The article also discusses the application of the latest derivatizing reagents for histamine and various analytical techniques.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Fangfang Zhu, Honglong Liu, Wenqing Zhang, Chuanyuan Du, Hao Zhu, Xiaodi Du, Xiaosong Hu, Yawei Lin
Summary: A novel BSMAD derivative was synthesized for the quantitative analysis of BAs in alcoholic beverages, and a HPLC method with low detection limit and excellent repeatability was successfully established. The method was applied for BA determination in various types of alcoholic beverages with satisfactory recoveries, providing a reliable and sensitive approach for future studies.
Review
Chemistry, Analytical
Justyna Piechocka, Rafal Glowacki
Summary: This overview summarizes all documented analytical methods for the quantification of homocysteine thiolactone (HTL) in biological samples, with a focus on pre-analytical treatment strategies and separation techniques. The review also discusses the advantages, pitfalls, and limitations of the methods, as well as the origin and main metabolites of HTL, and the association between civilization diseases and sulfur-containing compounds' metabolism disorders.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Gege Song, Zhiqiao Zou, Ailing Feng, Chenbiao Li, Meijuan Liang, Fuan Wang, Xiaoqing Liu
Summary: In this study, a flexible and portable light-up aptameric sensor was developed for smartphone-based point-of-care testing. By manipulating the affinity and dynamic response of designer DNA molecules, detection of serotonin was achieved with high sensitivity and selectivity. The study also demonstrated the versatility of the engineered aptameric molecular probes for different sensing applications.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Xinglei Zhang, Yuan Xu, Ting Li, Hui Li, Zhendong Yu, Lili Song, Jiaquan Xu
Summary: In this study, electrochemical mass spectrometry (EC-MS) combined with in situ electrospray ionization mass spectrometry (ESI-MS) was used to directly analyze heavy metal speciations in suspended particulate matter (SPM) in water. Four types of speciations were obtained through water and methanol extraction and sequential extraction. Compared with traditional methods, this method has the advantages of no complex matrix separation process, less sample consumption, and high analysis throughput, making it suitable for advanced applications in environmental monitoring and risk assessment.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2023)
Review
Chemistry, Analytical
Clarisse Gosset-Erard, Frederic Aubriet, Emmanuelle Leize-Wagner, Yannis-Nicolas Francois, Patrick Chaimbault
Summary: This review provides an overview of the online hyphenation of FT-ICR MS with separation methods, highlighting its advantages and challenges. While FT-ICR MS requires time to achieve high resolving power and accuracy, chromatography and electrophoresis offer transient peaks. The combination of these techniques balances their strengths and weaknesses, making it valuable for applications such as untargeted Omics. Although implementing FT-ICR MS with online separation methods can be challenging, it has shown unparalleled results and raises questions about its future with advancing technology.
Article
Medical Laboratory Technology
Kasper Munch Lauridsen, Helle Pilgaard Kristiansen, Anne Winther-Larsen
Summary: This study aimed to establish pediatric reference intervals (RIs) of hemoglobin fractions using high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) in children and adolescents aged 1-18 years. The study found that HbA2 RI was 2.00-2.90% by HPLC and 2.2-3.0% by CE, and partitioning by age was suggested by CE but not by HPLC.
CLINICA CHIMICA ACTA
(2023)
Review
Biochemical Research Methods
Le Peng, Xin Gao, Long Wang, Aiqiang Zhu, Xiang Cai, Pian Li, Wenlong Li
Summary: This paper presents a systematic literature review of the application of Design of experiment (DoE) techniques in the development of chromatographic analysis methods. It introduces the common designs and implementation steps of DoE, discusses the recent studies on the application of DoE techniques in various chromatographic techniques, and provides insights for future research in different chromatographic techniques fields.
Article
Biochemical Research Methods
Mohamed Adel Ahmed, Joselito P. Quirino
Summary: The study discusses the in-line sample concentration by micelle to cyclodextrin stacking in open-tubular liquid chromatography with UV detection. Factors influencing MCDS, such as type and concentration of CD, concentration of CTAB, were studied to achieve higher sensitivity and linear range under optimized conditions. Application to real samples showed comparable results to high-performance liquid chromatography-UV method.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Henri Ravald, Susanne K. Wiedmer
Summary: This article describes the use of lipid vesicles and liposomes as cell membrane models for investigating interactions between lipid membranes and 0-blockers in capillary electromigration and liquid chromatography techniques. The use of commercial intravenous lipid emulsions and their interactions with 0-blockers are also discussed. Different instrumental techniques for these purposes are introduced, and other methodologies for studying 0-blocker interactions with lipid membranes are compared.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Multidisciplinary
Arixin Bo, Yawei Liu, Bjoern Kuttich, Tobias Kraus, Asaph Widmer-Cooper, Niels de Jonge
Summary: Self-assembly of nanoscale structures at liquid-solid interfaces plays a crucial role in industrial processes and natural phenomena. The conventional theory is oversimplified, and in situ observations using liquid-phase scanning transmission electron microscopy are required. It has been found that modifying the molecular coating of the substrate can control the self-assembly structures of nanoparticles.
ADVANCED MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Yutong Gao, Youshuang Zhou, Xiangyun Xu, Chungui Chen, Bijin Xiong, Jintao Zhu
Summary: A facile method is demonstrated to fabricate oriented colloidal crystals from polymer-tethered gold nanoparticles through capillary assembly. The orientation structure of the crystals is mainly determined by the chain length of polymer ligands and size of gold nanoparticles.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)