期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1216, 期 31, 页码 5838-5848出版社
ELSEVIER
DOI: 10.1016/j.chroma.2009.06.023
关键词
Liposomes; Size distribution; Size exclusion chromatography; Cryo-transmission electron microscopy; Dynamic light scattering
Detergent removal from mixed micelles was combined with preparative size exclusion chromatography (SEC) on Sephacryl S 500 HR to prepare unilamellar and spherical liposomes of defined sizes between 50 and 100 nm with a very narrow size distribution (RSD of vesicle diameter between 13% and 25%). For neutral phosphatidylcholine and negatively charged phosphatidylcholine/phosphatidylglycerol liposome preparations, efficient sizing at the preparative scale was demonstrated by analyzing isolated SEC peak fractions with cryo-transmission electron microscopy and dynamic light scattering. The number-weighted average vesicle diameters obtained using both methods are in very good agreement for fractions of low polydispersity. (C) 2009 Elsevier B.V. All rights reserved.
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