Article
Chemistry, Analytical
Ban Khalil Ail
Summary: The presence of mycotoxins in food poses potential health risks, making it crucial to monitor and develop effective methods for removal. Researchers have explored the use of sol-gel immobilization for separating toxins from complex matrices, demonstrating its versatility and potential for broad applications.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Izabela Podgorska-Kryszczuk, Ewa Solarska, Monika Kordowska-Wiater
Summary: This study used selected yeast strains to reduce the levels of mycotoxins produced by Fusarium culmorum, F. graminearum, and F. poae on wheat grain and bread. The results showed that these yeast isolates can be efficient mycotoxin decontamination agents in the food industry.
Article
Agriculture, Dairy & Animal Science
Jong Young Ahn, Jongkeon Kim, Da Hyeon Cheong, Hyosun Hong, Jin Young Jeong, Beob Gyun Kim
Summary: This study aimed to determine the efficacy of mycotoxin sequestering agents for aflatoxin B1, deoxynivalenol, and zearalenone using an in vitro method. Results showed that most sequestering agents effectively bound to aflatoxin B1, with only activated charcoal showing significant effects on deoxynivalenol and zearalenone.
Article
Biotechnology & Applied Microbiology
Marina H. Boshra, Ghadir S. El-Housseiny, Mohammed M. S. Farag, Khaled M. Aboshanab
Summary: This study aimed to validate two common methods for monitoring MTs levels in various food categories. Results showed no significant differences between the ELISA and immunoaffinity fluorometric analysis in the detection of MTs. However, significant differences were observed among different food categories, highlighting the need for strict control measures to minimize the risk of MTs adverse effects.
Article
Agronomy
Jan Marczuk, Piotr Brodzki, Adam Brodzki, Katarzyna Glodkowska, Karolina Wrzesniewska, Nikodem Brodzki
Summary: The study aimed to evaluate the impact of mycotoxin-contaminated feed on protein metabolism in cows and investigate the effectiveness of a mycotoxin deactivator product (MDP). The experimental group of 10 cows were fed a total mixed ration (TMR) containing mycotoxins, while the control group were fed TMR without mycotoxins. The MDP Mycofix Plus was added to the TMR of the experimental group for 90 days. The results showed that mycotoxicosis had a negative impact on protein metabolism in cows, but the use of MDP improved these processes and overall cow health.
Article
Food Science & Technology
Congning Zhai, Yangguang Yu, Jun Han, Junqiang Hu, Dan He, Hongyin Zhang, Jianrong Shi, Sherif Ramzy Mohamed, Dawood H. Dawood, Gang Wang, Jianhong Xu
Summary: In this study, an anaerobic ZEN-degrading bacterium named F39 was isolated from animal intestinal contents and identified as Clostridium sporogenes. F39 showed a high efficiency in degrading ZEN and converting it into alpha- and beta-zearalenol, reducing the toxicity of degradation products. Additionally, F39 exhibited higher degradation efficiency in wheat-based feedstuffs compared to other feedstuffs, and the efficiency was pH related.
Article
Plant Sciences
Sean P. O'Mara, Karen Broz, Erin M. Schwister, Lovepreet Singh, Yanhong Dong, J. Mitch Elmore, H. Corby Kistler
Summary: This study reveals that the deletion of membrane-bound transporters in the plant pathogenic fungus Fusarium graminearum leads to reduced accumulation of the mycotoxin deoxynivalenol (DON) and decreased disease symptoms on wheat. The ATP-binding cassette (ABC) transporter gene Abc1 plays a major role in DON accumulation and virulence reduction. Heterologous expression of F. graminearum transporters in yeast confirms Abc1 as a major mechanism of DON resistance and suggests the involvement of multiple transporters in resistance to other compounds. These findings provide valuable insights for developing novel management techniques for Fusarium head blight and other destructive plant diseases.
Article
Chemistry, Applied
Hua Cui, Songshan Wang, Xu Yang, Wei Zhang, Mengze Chen, Yu Wu, Sen Li, Li Li, Di Cai, Baoyuan Guo, Jin Ye, Songxue Wang
Summary: Reliable prediction of mycotoxin contamination in post-harvest wheat is crucial for improving its quality and safety. The study found that water activity plays a significant role in the relationship between mycotoxins and CO2 production. Prediction models for DON and ZEN contamination were established based on CO2 concentration, moisture content, and temperature. These models, combined with multi-parameter integrated sensors, formed an early warning system to minimize the risk of mycotoxin contamination in post-harvest wheat.
Article
Food Science & Technology
Xing Qin, Xiaoyun Su, Tao Tu, Jie Zhang, Xiaolu Wang, Yaru Wang, Yuan Wang, Yingguo Bai, Bin Yao, Huiying Luo, Huoqing Huang
Summary: This study demonstrates that BsDyP is a promising candidate for the simultaneous degradation of multiple mycotoxins in animal feed and food, with lower toxicities of its degradation products.
Article
Microbiology
Valentina Scarpino, Massimo Blandino
Summary: Enhancing FHB resistance in wheat is an effective method to reduce mycotoxin contamination. The study found that wheat genotypes with high deoxynivalenol tolerance can minimize sanitary risks, with the most FHB resistant genotypes showing the lowest contamination levels of various mycotoxins. An inverse relationship between deoxynivalenol amount and ratios of other mycotoxins was observed across all wheat cultivars and years.
Article
Microbiology
Alexander A. Stakheev, Denis Erokhin, Ekaterina A. Meleshchuk, Oleg D. Mikityuk, Natalia Statsyuk
Summary: This study reveals that the main pathway for inhibition of DON and ZEN biosynthesis is the transcriptional regulation of genes involved in the G-protein-cAMP-PKA signaling pathway. These findings contribute to a better understanding of the genetic mechanisms underlying mycotoxin production and its regulation.
Article
Food Science & Technology
Maozhen Qu, Shijie Tian, Hengjie Yu, Da Liu, Chao Zhang, Yingchao He, Fang Cheng
Summary: Deoxynivalenol and zearalenone contamination in maize poses a threat to food safety. This study developed a cost-effective and non-destructive method for classifying contaminated maize. Texture and color features were extracted using visible light imaging and polarized light imaging, and six classification models were established. The results showed that the optimal detection surface was the endosperm side, and the SVM model achieved a classification accuracy of 88.1%. Further experiments verified the effectiveness of this method in identifying different contamination levels and reducing testing costs.
Article
Chemistry, Applied
Mobing Chen, Wei Liu, Ping Xiang, Linhao Xu, Yiling Tang, Ran Kong, Shuo Yang, Hui Yan, Bin Di
Summary: A sensitive LC-MS/MS method was developed to determine 17 mycotoxins in human urine. The method employed a two-step liquid-liquid extraction using ethyl acetate: acetonitrile (7:1) with good extraction recovery. The LOQs for all mycotoxins were in the range of 0.1-1 ng/mL. The method showed high intra-day and inter-day accuracy and precision. It was successfully applied to analyze the urine levels of 17 mycotoxins in 42 volunteers, with deoxynivalenol and zearalenone detected in a subset of samples.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2023)
Article
Plant Sciences
Jana Chrpova, Heinrich Grausgruber, Verena Weyermann, Maria Buerstmayr, Jana Palicova, Jana Kozova, Martina Travnickova, Quynh Trang Nguyen, Jose Esteban Moreno Amores, Hermann Buerstmayr, Dagmar Janovska
Summary: Modern spelt varieties with reduced plant height showed higher susceptibility to Fusarium head blight and higher contamination of grains with deoxynivalenol (DON), while old landraces and varieties had higher resistance and lower mycotoxin levels. These findings can aid in selecting suitable parental material for breeding spelt with improved FHB resistance.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Food Science & Technology
Agnieszka Tkaczyk, Piotr Jedziniak, Lukasz Zielonka, Michal Dabrowski, Piotr Ochodzki, Adrianna Rudawska
Summary: This study successfully utilized LC-MS/MS methods to determine mycotoxin biomarkers of exposure in pigs. The levels of biomarkers in urine and serum correlated well with toxin intake, demonstrating their suitability for exposure monitoring. Urine is suitable for monitoring multiple mycotoxin exposures, while serum may be used to monitor specific mycotoxin exposures. Additionally, both matrices are suitable candidates to monitor prolonged OTA exposure in pigs.
Article
Biochemistry & Molecular Biology
Samuel M. Meier-Menches, Katja Zappe, Andrea Bileck, Dominique Kreutz, Ammar Tahir, Margit Cichna-Markl, Christopher Gerner
Article
Biochemistry & Molecular Biology
Georg Pretsch, Nasim Sanadgol, Roman Smidak, Jana Lubec, Volker Korz, Harald Hoeger, Katja Zappe, Margit Cichna-Markl, Gert Lubec
Article
Biochemistry & Molecular Biology
Eva Nora Bukosza, Christoph Kornauth, Karin Hummel, Helga Schachner, Nicole Huttary, Sigurd Krieger, Katharina Noebauer, Andre Oszwald, Ebrahim Razzazi Fazeli, Klaus Kratochwill, Christoph Aufricht, Gabor Szenasi, Peter Hamar, Christoph A. Gebeshuber
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Fisheries
Simon Menanteau-Ledouble, Michael Gotesman, Ebrahim Razzazi-Fazeli, Sven M. Bergmann, Mansour El-Matbouli
JOURNAL OF FISH DISEASES
(2020)
Article
Multidisciplinary Sciences
Simon Menanteau-Ledouble, Katharina Noebauer, Ebrahim Razzazi-Fazeli, Mansour El-Matbouli
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Donatella Tesei, Felice Quartinello, Georg M. Guebitz, Doris Ribitsch, Katharina Noebauer, Ebrahim Razzazi-Fazeli, Katja Sterflinger
SCIENTIFIC REPORTS
(2020)
Article
Chemistry, Multidisciplinary
Samuel M. Meier-Menches, Benjamin Neuditschko, Katja Zappe, Martin Schaier, Marlene C. Gerner, Klaus G. Schmetterer, Giorgia Del Favero, Riccardo Bonsignore, Margit Cichna-Markl, Gunda Koellensperger, Angela Casini, Christopher Gerner
CHEMISTRY-A EUROPEAN JOURNAL
(2020)
Article
Multidisciplinary Sciences
Maria Kaltenbrunner, Walter Mayer, Kirsten Kerkhoff, Rita Epp, Hermann Rueggeberg, Rupert Hochegger, Margit Cichna-Markl
SCIENTIFIC REPORTS
(2020)
Review
Cell Biology
Katja Zappe, Margit Cichna-Markl
Letter
Allergy
Sandra Pfeiffer, Peter Sandler, Marianne Raith, Mariona Pascal, Rosa Maria Munoz-Cano, Clara San Bartolome, Katharina Noebauer, Santiago Quirce, Ebrahim Razzazi-Fazeli, Margarete Focke-Tejkl, Katja Sterflinger, Ines Swoboda
Article
Food Science & Technology
Kristina Gense, Verena Peterseil, Alma Licina, Martin Wagner, Margit Cichna-Markl, Stefanie Dobrovolny, Rupert Hochegger
Summary: The study introduces a DNA metabarcoding method for identifying bivalve species in foodstuffs, showing high accuracy in detecting both major and minor components. It is suitable for complex and processed foods, providing a useful tool for food authentication.
Article
Food Science & Technology
Barbara Druml, Steffen Uhlig, Kirsten Simon, Kirstin Frost, Karina Hettwer, Margit Cichna-Markl, Rupert Hochegger
Summary: A real-time PCR assay has been developed to identify and quantify roe deer in food products, aimed at detecting potential adulteration of game meat with cheaper alternatives. Interlaboratory ring trials across several EU Member States have shown the assay to be reliable and accurate for the detection and quantification of roe deer in raw meat samples. Performance characteristics such as amplification efficiency, LOD95%, repeatability, reproducibility, and accuracy of quantitative results have been evaluated with satisfactory results, making this PCR assay a valuable tool for food authentication purposes.
Article
Food Science & Technology
Laura Preckel, Claudia Brunen-Nieweler, Gregoire Denay, Henning Petersen, Margit Cichna-Markl, Stefanie Dobrovolny, Rupert Hochegger
Summary: DNA metabarcoding is an effective method for routine analysis of mammalian and poultry species in food and pet food products, showing consistent results with PCR analysis. It is particularly suitable for detecting unexpected species and is a cost-effective alternative, especially for analyzing pet food products with high potential for adulteration.
Article
Food Science & Technology
Stefanie Dobrovolny, Steffen Uhlig, Kirstin Frost, Anja Schlierf, Kapil Nichani, Kirsten Simon, Margit Cichna-Markl, Rupert Hochegger
Summary: Meat species authentication in food is often conducted using genetic variation detection. DNA metabarcoding, a novel method that can simultaneously identify various species, shows great potential in the field and could be harmonized across EU Member States.
Editorial Material
Food Science & Technology
Margit Cichna-Markl, Isabel Mafra
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)