期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1216, 期 31, 页码 5903-5910出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2009.06.014
关键词
Thermochemolysis; Trimethylsulfonium hydroxide (TMSH); Tetramethylammonium hydroxide (TMAH); Solids injector; Injection port; Lignin markers
资金
- Natural Sciences and Engineering Research Council of Canada
- Memorial University
A simple and direct approach was developed for thermochemolytic analysis of a wide range of biomolecules present in plant materials using an injection port of a gas chromatograph/mass spectrometer (GC/MS) and a novel solids injector consisting of a coiled stainless steel wire placed inside a modified needle syringe. Optimum thermochemolysis (or Thermally Assisted Hydrolysis/Methylation) was achieved by using a suitable methanolic solution of trimethylsulfonium hydroxide (TMSH) or tetramethylammonium hydroxide (TMAH) with an injection port temperature of 350 degrees C. Intact, methylated flavonoids, saccharides, phenolic and fatty acids, lignin dimers and diterpene resin acids were identified. Samples include tea leaves, hemicelluloses, lignin isolates and herbal medicines. Unexpected chromatographic results using TMAH reagent revealed the presence of intact methylated trisaccharides (658 Da) and structurally informative dimer lignin markers. (C) 2009 Elsevier B.V. All rights reserved.
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