期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1216, 期 26, 页码 5155-5159出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2009.04.081
关键词
Microchip electrophoresis; Chemiluminescence detection; N-(4-aminobutyl)-N-ethylisoluminol; Biogenic amines; Human urine
资金
- National Natural Science Foundations of China (NSFC) [20665002, 20875019]
- US National Institutes of Health [S06GM08047]
A highly sensitive microchip electrophoresis (MCE) method with chemiluminescence (CL) detection was developed for the determination of biogenic amines including agmatine (Agm), epinephrine (E), dopamine (DA), tyramine, and histamine in human urine samples. To achieve a high assay sensitivity, the targeted analytes were pre-column labeled by a CL tagging reagent, N-(4-aminobutyl)-N-ethylisoluminol (ABEI). ABEI-tagged biogenic amines after MCE separation reacted with hydrogen peroxide in the presence of horseradish peroxidase (HRP), producing CL emission. Since no CL reagent was added to the running buffer, the background of the CL detection was extremely low, resulting in a significant improvement in detection sensitivity. Detection limits (S/N=3) were in the range from 5.9 x 10(-8) to 7.7 x 10(-8) M for the biogenic amines tested, which were at least 10 times lower than those of the MCE-CL methods previously reported. Separation of a urine sample on a 7 cm glass/poly(dimethylsiloxane) (PDMS) microchip channel was completed within 3 min. Analysis of human urine samples found that the levels of Agm, E and DA were in the ranges of 2.61 x 10(-7) to 4.30 x 10(-7) M, 0.81 x 10(-7) to 1.12 x 10(-7) M, and 8.76 x 10(-7) to 11.21 x 10(-7) M (n=4), respectively. (C) 2009 Elsevier B.V. All rights reserved.
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