期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1216, 期 29, 页码 5614-5618出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2009.05.067
关键词
Cyanamide; Fertilizer; Hairy vetch; 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate; Lime nitrogen; Soil; Ultra-high-pressure liquid chromatography
A rapid and simple method for determination of cyanamide in fertilizer, soil and plants has been developed. In this method, cyanamide is extracted with 2% acetic acid and the extract separated by centrifugation. It is then purified by passing through a membrane filter. The extract was derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate and the derivatized compound separated by ultra-high-pressure liquid chromatography. it is then detected with a UV detector at 260 nm by the same method as is used for amino acid analysis. The proposed method is fast, simple and cheap and also has good selectivity and sensitivity for the determination of cyanamide in a wide range of biotic and abiotic materials. (C) 2009 Elsevier B.V. All rights reserved.
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