期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1216, 期 11, 页码 2179-2184出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2008.05.068
关键词
Biological fingerprinting chromatogram analysis; Cortex Pseudolarix; Radix Stephaniae; MCF-7; Multidrug resistance
资金
- National Natural Science Foundation of China [90709021]
- Dalian Young Scientific and Technological Talents Fund [2007J23JH026]
- DLFU [015605]
- Key Laboratory of Industrial Ecology and Environmental Engineering of China Ministry of Education [0704]
- CAS [KJCX2.YW.HO9]
An efficient and convenient method, biological Fingerprinting chromatogram analysis is presented. which is applied to the comparison or finger printing chromatograms of the extracts of Chinese herbal medicines after the interaction with biological systems (cell, DNA, protein, etc.). The method was established for the purpose of screening and analysis of the multiple bioactive compounds in herbal medicines. In this work, microdialysis sampling combined with high performance liquid chromatography-mass spectrometry (HPLC-MS) was Studied for binding property of MCF 7 and multidrug resistant MCF-7 cell systems. The results showed that pseudolaric acid A (PAA) and pseudolaric acid B (PAB) in the cortex of Pseudolarix kaempferi (Lamb.) Gorden can easily bind to the MCF-7 cells ranging from 0 to 16.3% (PAA) and from 0 to 35.7% (PAB), and another compound, tetrandrine (TET) from the root of Stephania tetrandrae S. Moore, showed higher binding activity with multidrug resistant MCF-7 cells ranging from 0 to 39.9%. (C) 2008 Elsevier B.V. All rights reserved.
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