Article
Chemistry, Analytical
Petr Kozlik, Jana Vaclova, Kveta Kalikova
Summary: In this study, three recently developed HILIC columns were characterized in detail to compare their peptide retention mechanisms, revealing a multimodal retention mechanism in systems with these stationary phases. By balancing the properties of analytes and stationary phases, satisfactory retention and peak shape of peptides can be achieved. Buffer concentration and pH optimization was confirmed to be an effective tool for manipulating retention and peak symmetry, enhancing the potential applications of these columns in the analysis of diverse compounds.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemical Research Methods
Matthew J. Sorensen, Mellie June Paulines, Todd D. Maloney
Summary: This study evaluated the orthogonality between ion-pair reversed phase (IP-RP), anion exchange (AEX), and hydrophilic interaction liquid chromatography (HILIC) for the separation of oligonucleotides. The results showed that coupling HILIC with either AEX or IP-RP provided the highest degree of orthogonality.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Ye Shu, Tong Chen, Tao Liu, Hechen Xu, Jinjin He, Zhihui Cai, Wanning Zhu, Zihan Zhao, Jun Jiang
Summary: In this study, spherical silica was modified with imidazole, amide, and sulfonic acid. The modified silica was used to create novel polymerization stationary phases for hydrophilic interaction liquid chromatography (HILIC). Various characterization techniques were employed to analyze the stationary phases, and their performance was evaluated using high performance liquid chromatography. The HILIC stationary phases exhibited excellent retention properties and selectivity compared to the conventional C18 column. They were successfully applied for the separation of sulfonamides, flavonoids, and Fructus Ligustri Lucidi extracts. Overall, the prepared surface polymeric stationary phases showed great potential for the analysis of polar components and complex samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Marta Passamonti, Chiem de Roos, Peter J. Schoenmakers, Andrea F. G. Gargano
Summary: In this study, polymerization mixture was optimized to synthesize poly(acrylamide-co-N,N'-methylenebisacrylamide) monolithic stationary phases for HILIC of intact proteins. The monolithic structure exhibited a permeable and polar stationary phase suitable for HILIC. The monolith showed superior separation performance and lower ion interaction compared to silica-based particulate amide phase, enabling successful hyphenation with high-resolution mass spectrometry in HILIC of intact proteins.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Isabelle Kohler, Michel Verhoeven, Rob Haselberg, Andrea F. G. Gargano
Summary: Proteomics and metabolomics have become increasingly important in clinical applications, with RPLC-MS and HILIC-MS techniques playing crucial roles in biomarker discovery and disease diagnosis. While RPLC-MS and HILIC-MS complement each other in resolving the complexity of proteome and metabolites, they also have technical limitations and potential solutions that need to be addressed.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Zongying Li, Shuxiang Li, Feifang Zhang, Huiliang Geng, Bingcheng Yang
Summary: The novel amide functionalized polar stationary phase exhibits favorable hydrophilicity, excellent separation ability, and outstanding hydrolytic stability, with minimal baseline drift under gradient elution and good selectivity in the separation of isomers and homologue sugars.
Article
Nanoscience & Nanotechnology
Arunima Rajan, Niroj Kumar Sahu
Summary: Magnetically induced hyperthermia using iron oxide nanoparticles presents an effective alternative to traditional cancer treatments, with monodisperse oleate-coated magnetite nanorods demonstrating promising performance in biological studies.
ACS APPLIED NANO MATERIALS
(2021)
Article
Biochemical Research Methods
Katarina Molnarova, Ales Duris, Tomas Jecmen, Petr Kozlik
Summary: Glycoproteomics, a challenging field due to glycosylation heterogeneity, benefits from hydrophilic interaction liquid chromatography for glycopeptide separation. Comparing HILIC columns in human immunoglobulin G glycopeptide separation, it was found that different columns exhibit higher separation power for different glycoforms and can effectively elucidate the structure of isomeric glycoforms. The retention mechanism for HILIC columns is discussed based on determined chromatographic parameters.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Qianying Sheng, Meiyan Liu, Minbo Lan, Guangyan Qing
Summary: Hydrophilic interaction liquid chromatography (HILIC) is an attractive complementary method to reversed phase LC for the separation of hydrophilic or strongly polar substances. The advancements in HILIC have significantly improved sensitivity, analyte coverage, throughput, analysis speed, and resolution in bio-separation and bio-analytical chemistry.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Xiaoqing Wang, Jian Cui, Jin Zhou, Shuo Wang, Yiming Gu, Xiaowei Liu, Shudong Wang
Summary: Vinyl modified silica was synthesized through a silane coupling reaction and a poly-acrylamide modified silica (PAM-SIL) stationary phase was prepared via free radical polymerization. The separation performance of the stationary phases could be regulated by controlling the polymer structure, showing potential for future separation and analysis applications.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Kaixing Luo, Yu Luo, Yanjuan Liu, Yuefei Zhang, Wei Chen, Zhengwu Bai, Sheng Tang
Summary: In this work, a new strategy was adopted to simultaneously graft two different functional ligands onto the silica surface, resulting in a carbonyldiimidazolium/dodecyl dual-functionalized silica stationary phase (Sil-CDI-DD) using recycled commercial C18 columns as raw materials. The obtained Sil-CDI-DD exhibited enhanced separation selectivity and efficiency, providing multiple interaction sites for diverse target analytes and enabling the favorable resolution of hydrophilic nucleosides/nucleobases and weak polar environmental endocrine disruptors.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Jing Li, Danye Qiu, Feng Li, Jingwu Kang
Summary: A method was reported for preparing a poly(N-vinylpyrrolidone-co-pentaerythritol triacrylate copolymerization)-based monolithic capillary column for the separation of polar small molecular weight compounds with nano-liquid chromatography in hydrophilic interaction chromatography mode. The monolithic columns were prepared by copolymerization of N-vinylpyrrolidone and pentaerythritol triacrylate in a binary porogenic agent consisting of methanol and water. The polymerization solution was optimized for column permeability, theoretical plate number, asymmetric factor, and retention factor. The N-vinylpyrrolidone-based hydrophilic interaction chromatography stationary phase showed satisfactory separation selectivity for polar test analytes.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Sumit K. Singh, Kelvin H. Lee
Summary: Glycosylation is a critical quality attribute of monoclonal antibody therapeutics. Hydrophilic interaction liquid chromatography-mass spectrometry (HILIC-MS) is a valuable technology for glycosylation characterization. Traditional glycan analysis based on glucose units (GU) and accurate mass (AM) is not suitable for complex samples or high-throughput applications. This study presents a two-step strategy for targeted glycan analysis and its application in different scenarios.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Honorine Lardeux, Davy Guillarme, Valentina D'Atri
Summary: Hydrophilic interaction chromatography (HILIC) is proposed as an alternative to ion-pairing reversed-phase chromatography (IP-RPLC) for oligonucleotide (ON) analysis. This study evaluates the potential of seven zwitterionic HILIC columns and other columns for ON analysis, and highlights the importance of understanding column characteristics (retention and selectivity) when selecting a stationary phase for specific ON applications.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Qi Wu, Xiudan Hou, Haitao Lv, Hui Li, Liang Zhao, Hongdeng Qiu
Summary: The novel octadecylamine-derived CDs, C18-CDs, were successfully designed, synthesized, and applied in RPLC/HILIC mixed-mode chromatography, showing good separation performance for both hydrophobic and hydrophilic compounds. The C18-CDs/SiO2 column demonstrated efficient separation for various compounds under different modes, indicating its potential for further research in mixed-mode chromatography.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)