Article
Chemistry, Analytical
Apostolia Tsiasioti, Paraskevas D. Tzanavaras
Summary: In this study, a new method called Pulsed-post column derivatization (Pulsed-PCD) was developed for the determination of thiols in yeast samples. The method involved using 100% aqueous mobile phase for chromatographic separation and on-line reaction with a derivatizing reagent. Spectrophotometric detection was carried out and the method was validated for endogenous content determination. The proposed method was found to be greener in terms of chemical usage and waste production compared to a corroborative method based on classical PCD.
Article
Environmental Sciences
Yu-Ru Lin, Yeh-Fang Hu, Chih-Yang Huang, Huai-Ting Huang, Zhen-Hao Liao, An-Ting Lee, Yu-Sheng Wu, Fan-Hua Nan
Summary: Zeolite, oyster shell powder (OSP), and humic acid (HA) showed high affinity for malachite green (MG) and its metabolite leucomalachite green (LMG) in both freshwater and seawater, while Chloride dioxide (ClO2) exhibited greater efficiency for MG and LMG degradation. These water treatment agents have the potential to reduce the amount of hazardous MG and LMG residue in aquatic environments.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2022)
Article
Chemistry, Analytical
Brittany Widner, Melissa C. Kido Soule, Frank Xavier Ferrer-Gonzalez, Mary Ann Moran, Elizabeth B. Kujawinski
Summary: Utilizing pre-extraction derivatization and ultrahigh performance liquid chromatography electrospray ionization tandem mass spectrometry, targeted dissolved metabolites in seawater and saline culture media were successfully detected and quantified. This quantitative method has been optimized for 73 common metabolites, with 50 requiring derivatization for quantification due to low extraction efficiencies.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Wenwei Wu, Qifeng Sun, Tiansong Li, Kexin Liu, Yan Jiang, Yi Wang, Yongjie Yang
Summary: The bispecific aptamers against MG and LMG were isolated using Capture-SELEX, and their specificity and affinity were confirmed. This study provides novel probes for the development of multi-residues rapid detection and opportunities for multiple target aptamer discovery.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Biochemical Research Methods
Apostolia Tsiasioti, Constantinos K. Zacharis, Paraskevas D. Tzanavaras
Summary: In this article, a new approach to reduce the consumption of reagents in liquid chromatography coupled to online post column derivatization is proposed. The proposed method, called Pulsed-Post Column Derivatization (Pulsed-PCD), introduces reagents as well-defined pulses at microliter levels. The robustness of the procedure was evaluated and the method was successfully applied to determine histidine in human urine.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Alexandros Pavlidis, Apostolia Tsiasioti, Paraskevas D. Tzanavaras
Summary: In this study, an HPLC method coupled with on-line post column derivatization was proposed for the simultaneous determination of glutathione and its disulfide in flour samples. The efficiency, stability and compatibility of analyte extraction from the sample matrix were investigated. By using o-phthalaldehyde in a high alkaline medium as the post column reaction system, both analytes could be determined in a single run with reasonable separation times. Fluorimetric detection allowed for determination at low levels without interference from endogenous compounds. The method exhibited LOD values of 1.2-1.3 nmol g-1 and precision better than 7.2% in all cases. Analysis of commercially available flour samples revealed the presence of glutathione and its disulfide in the ranges of 29-243 and 8-84 nmol g-1, respectively, with only one rice flour sample having levels of GSH below the LOQ of the method.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Applied
Apostolia Tsiasioti, Anastasia-Stella Zotou, Paraskevas D. Tzanavaras
Summary: The study successfully developed a method for determining glutathione and its disulfide using liquid chromatography with on-line postcolumn derivatization and fluorimetric detection. The method showed high sensitivity and throughput, with accurate determination of analytes content in various food samples. It is considered a reliable and promising analytical tool for estimating the redox status of glutathione in different food matrices.
Article
Biochemical Research Methods
Apostolia Tsiasioti, Eirini Georgiadou, Constantinos K. Zacharis, Paraskevas D. Tzanavaras
Summary: In this study, an optimized and validated method was used to monitor glutathione disulfide (GSSG) in human saliva samples. The results showed accurate quantification of GSSG in the samples, with a concentration range of 100-2000 nmol L-1 and a LOD of 20 nmol L-1. The stability of the samples was ensured by blocking reduced glutathione with N-ethylmaleimide.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Chemistry, Analytical
Qiao-Lian Duan, Hai-Wei Shi, Li Tan, Zhen Liu, Qing Huang, Wei Shen, Ling Cao, Hian Kee Lee, Sheng Tang
Summary: A new ultrahigh-performance supercritical fluid chromatography (UHPSFC) method was developed to solve the difficult separation problem of various biogenic amines (BAs) in gentamicin. The method achieved complete separation of 10 BAs using dansyl chloride derivatization and UHPSFC system. It demonstrated high sensitivity and accuracy, and met the quantification limits required for impurity determination.
ANALYTICAL CHEMISTRY
(2022)
Article
Parasitology
Lee Phone Youth Zen, Meng Yee Lai, Syaza Izzati binti Rozlan, Mohd Hafizi Abdul Hamid, Jenarun Jelip, Rose Nani Mudin, Yee Ling Lau
Summary: This study proposes the use of colorimetric dyes (malachite green, phenol red, and xylenol orange) in the LAMP assay to simplify current end-point detection methods. The optimal concentration of dyes was determined to be 125μM MG at pH 8.8, which produced the most obvious color change. MG-LAMP showed 100% sensitivity and specificity when tested on 71 clinical blood samples. This method is simple, affordable, and suitable for malaria diagnosis in resource-limited settings.
EXPERIMENTAL PARASITOLOGY
(2022)
Article
Food Science & Technology
Thanh Duy Nguyen, Thuy Ngan Ha Nguyen, Tuan Kiet Ly, Quoc Hung Nguyen, Thanh Tho Le, Van Hai Chu, Tien Dat Nguyen, Dinh Vu Le
Summary: Optimization and validation of a method using ultra-performance liquid chromatography with fluorescence detection were performed for the simultaneous quantitation of aflatoxins in peanuts and raisins. The method offered advantages including short analysis time, simple sample handling, and reduced solvent consumption. The detection limits obtained were lower than those of other methods. The study can serve as a standard method for aflatoxin analysis in peanut and raisin matrices. The investigation showed that a significant percentage of peanut samples were contaminated with aflatoxins, while almost no aflatoxins were found in the raisin samples.
FOOD SCIENCE & NUTRITION
(2023)
Article
Chemistry, Applied
Apostolia Tsiasioti, Paraskevas D. Tzanavaras
Summary: In this study, naphthalene-2,3-dicarboxaldehyde (NDA) was used in Pulsed-PCD, a new concept for on-line post column derivatization (PCD) coupled with liquid chromatography. The use of expensive reagents in PCD was minimized by introducing the reagents into the mobile phase flow at precise timing. NDA-CN chemistry was applied to determine histamine in food samples, while two additional methods based on histamine reactions with o-phthalaldehyde (OPA) were developed. The consumption of PCD reagents was significantly reduced by using a 50 μl pulse (OPA/NDA + Buffer) in all three methods.
Article
Biochemistry & Molecular Biology
Kalliopi Papavasileiou, Apostolia Tsiasioti, Paraskevas D. Tzanavaras, Constantinos K. Zacharis
Summary: This study demonstrates the development, validation, and application of a new liquid chromatography post-column derivatization method for the determination of Colistin in human urine samples. The method was optimized using experimental design and validated using the total error concept. The proposed method showed satisfactory results and was successfully applied to the analysis of the drug in human urine samples.
Article
Chemistry, Analytical
Bert Vankeerberghen, Francois Parmentier, Gert Desmet
Summary: The efficiency of liquid chromatography separations can be greatly improved by using a bundle of parallel capillary tubes instead of packed bed columns. However, the presence of polydispersity caused by small differences in capillary diameter ruins this potential. The concept of diffusional bridging that introduces a cross talk between adjacent capillaries has been proposed to solve this issue, and this study provides the first experimental proof and validation of the theory behind it. The observed reduction in dispersion aligns well with theoretical predictions, suggesting that this theory could lead to the design of a new generation of chromatographic beds with unprecedented performance.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Hakan Alyuruk, Aynur Kontas, Oya Altay
Summary: Two reversed-phase HPLC methods were compared for molecular analysis of dissolved free monosaccharides in seawater, with the 2-amino benzamide method showing better results. A total of 12 monosaccharides could be quantified with detection limits between 1.2 and 11 nM and good repeatability. Additional studies using different column polarities may lead to even higher sensitivity and detection of more monosaccharides.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)