Article
Biochemical Research Methods
Annestasia Simbut John, Mohammad Mizwaruddin Sidek, Lee Yien Thang, Sabita Sami, Hui Yin Tey, Hong Heng See
Summary: This review provides a comprehensive overview of various online preconcentration techniques in nonaqueous capillary and microchip electrophoresis, including sample stacking, isotachophoresis, and micellar-based stacking. Attention is also given to multi-stacking strategies used for nonaqueous electrophoresis.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Andaravaas Patabadige Jude P. Vaas, Joselito P. Quirino
Summary: A stacking technique using CTAB and SDS was proposed to enhance the detection sensitivity of capillary zone electrophoresis (CZE) with UV detection. The method involves enriching and releasing analytes from the inner walls of a fused silica capillary by forming catanionic micelles, leading to improved detection sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Ziyan Yu, Yaxiao Liao, Jie Liu, Qin Wu, Yu Cheng, Ke Huang
Summary: A novel colorimetric approach for detecting kanamycin based on the aggregation of gold nanoparticles (AuNPs) induced by kanamycin is presented in this study. The sensor showed a linear range of 0.5-3 nM and a limit of detection of 0.11 nM under optimal conditions. It was tested by monitoring kanamycin in six food samples with satisfactory spiked recoveries, making it a desirable choice for fast and highly sensitive detection of kanamycin.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Petr Tuma, Tomas Hlozek, Blanka Sommerova, Dusan Koval
Summary: The study developed an electrophoretic stacking method for sensitive determination of three zwitterionic antiepileptics in human serum. The method showed a high sensitivity analysis under specific background electrolyte conditions.
Article
Biochemical Research Methods
Andaravaas Patabadige Jude P. Vaas, Raymond B. Yu, Joselito P. Quirino
Summary: Cyclodextrins are proposed as a pseudophase in pseudophase-to-pseudophase microextraction in capillary zone electrophoresis. This method, known as CD to admicelle ME, involves the injection of a dilute analyte solution into the capillary, forming admicelles at the interface. CD solution is then injected to release the analytes from the capillary surface, resulting in concentration of the analytes. The method shows enhanced sensitivity and has potential for analysis of urine samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Petr Tuma, Blanka Sommerova, Dusan Koval, Francois Couderc
Summary: In this study, capillary electrophoresis with contactless conductivity detection was employed to determine asymmetric and symmetric dimethylarginines in human plasma. The method is highly sensitive, accurate, and does not require derivatization processing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Analytical
Theo Lienard-Mayor, Myriam Taverna, Stephanie Descroix, Thanh Duc Mai
Summary: This study provides a comprehensive overview of different strategies for combining droplet-based sample handling and preparation with electrophoretic separation. It focuses on three principal interfacing strategies, including droplets in immiscible phases, digital microfluidics with electrowetting-on-dielectric principle, and inkjet droplet generation. Different instrumental developments, pros and cons of designs, and application demonstrations of droplet-interfaced electrokinetic strategies are discussed.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Kevin Jooss, Luis F. Schachner, Rachel Watson, Zachary B. Gillespie, Sarah A. Howard, Marcus A. Cheek, Matthew J. Meiners, Amin Sobh, Jonathan D. Licht, Michael-Christopher Keogh, Neil L. Kelleher
Summary: The study introduces a new platform for separating and characterizing whole nucleosomes and their modifications, aiding in understanding epigenetic regulatory mechanisms. The platform offers low sample requirements and costs, with high detection sensitivity.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Alexander Vladimirovich Ivanov, Mikhail Aleksandrovich Popov, Valery Vasil'evich Aleksandrin, Lubov Mikhailovna Kozhevnikova, Alexey Aleksandrovich Moskovtsev, Maria Petrovna Kruglova, Silina Ekaterina Vladimirovna, Stupin Victor Aleksandrovich, Aslan Amirkhanovich Kubatiev
Summary: A new approach for direct determination of GSH and GSSG in whole blood by capillary electrophoresis has been developed. The method includes GSH-stabilizing sample preparation, the use of an internal standard, and pH-mediated stacking. The method provides accurate and precise analysis of GSH and GSSG levels in blood samples.
Article
Chemistry, Analytical
Branislav Zabensky, Robert Bodor, David Makata, Roman Szucs, Marian Masar
Summary: An analytical test procedure for the direct determination of trace levels of perchlorate in drinking water by isotachophoresis combined with capillary zone electrophoresis was developed. The method exhibited excellent repeatability, linearity, and quantitation limit for perchlorate, making it more sensitive and robust than ion chromatography.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Biochemical Research Methods
Zhaohui Chu, Jingyi Chen, Jingzi Zhang, Qihui Xie, Fan Zhang, Qingjiang Wang
Summary: Efficient detection of pathogenic bacteria is crucial for food safety and preventing foodborne illnesses. In this study, a capillary electrophoresis coupled laser-induced fluorescence assay was developed for the detection of Escherichia coli by detecting its specific DNA. The method showed high sensitivity and was successfully applied to the detection of commercially available skim milk samples.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Ya-Ling Yu, Si-Chen Zhu, Min-Zhen Shi, Fang-Ming Liu, Jun Cao
Summary: An effective and sensitive method for analyzing arsenic species using co-electro-osmotic flow capillary zone electrophoresis and micelle-tosolvent stacking was developed. The method achieved high enrichment factors and low detection limits by utilizing a two-step stacking approach.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Mohamed Adel Ahmed, Joselito P. Quirino
Summary: The study discusses the in-line sample concentration by micelle to cyclodextrin stacking in open-tubular liquid chromatography with UV detection. Factors influencing MCDS, such as type and concentration of CD, concentration of CTAB, were studied to achieve higher sensitivity and linear range under optimized conditions. Application to real samples showed comparable results to high-performance liquid chromatography-UV method.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Anne-Lise Marie, Somak Ray, Shulin Lu, Jennifer Jones, Ionita Ghiran, Alexander R. Ivanov
Summary: A highly sensitive method for profiling N-glycans released from proteins using CZE-ESI-MS was developed and applied to glycan analysis of plasma and blood-derived isolates. The technique significantly improved detection sensitivity and resolution, allowing for qualitative and quantitative analysis of N-glycans in blood samples with unmatched depth compared to previous methods.
ANALYTICAL CHEMISTRY
(2021)
Article
Medicine, General & Internal
Katarzyna Pauter, Viorica Railean-Plugaru, Michal Zloch, Pawel Pomastowski, Malgorzata Szultka-Mlynska, Boguslaw Buszewski
Summary: The study aimed to characterize biofilm before and after antibiotic treatment using methods such as 16S rRNA gene sequencing and capillary electrophoresis. Results showed that antibiotics (amoxicillin and gentamicin) can alter the surface charge of bacterial cells, leading to cell clumping. Additionally, microscopic and spectrometry studies revealed that antibiotics inhibit cell wall synthesis and ribosomal protein synthesis in bacterial cells.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Food Science & Technology
Laura Gamiz-Gracia, Ana M. Garcia-Campana
Article
Agriculture, Dairy & Animal Science
Paloma Abad, Natalia Arroyo-Manzanares, Juan J. Ariza, Alberto Banos, Ana M. Garcia-Campana
Summary: The study showed that allium extract containing organosulfur compounds had a positive effect on productivity and gut microbiota of laying hens without affecting egg quality. Further research is needed to explore the effects of allium extract as a feed supplement.
Article
Agriculture, Multidisciplinary
Natalia Arroyo-Manzanares, Vicente Rodriguez-Estevez, Ana M. Garcia-Campana, Elena Castellon-Rendon, Laura Gamiz-Gracia
Summary: The occurrence of ergot alkaloids in feed samples in Spain appears to be lower than in other regions of Europe. All samples met current recommendations of the feed industry regarding practical limits for ergot alkaloids in pig feeds.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Chemistry, Analytical
Laura Carbonell-Rozas, Francisco J. Lara, Monsalud del Olmo Iruela, Ana M. Garcia-Campana
Summary: A simple and effective method using solid-liquid extraction followed by capillary liquid chromatography with diode array detection has been established for the extraction and determination of seven neonicotinoid insecticides in cereal samples. The method showed good linearity, repeatability, reproducibility, and trueness, with detection limits and quantification limits suitable for trace level determination in cereal samples below maximum residue limits. The advantages of miniaturized CLC technique and simplicity of solid-liquid extraction make this method a useful alternative for monitoring residues in cereal samples.
MICROCHEMICAL JOURNAL
(2021)
Article
Food Science & Technology
Laura Carbonell-Rozas, Choukri Khelifa Mahdjoubi, Natalia Arroyo-Manzanares, Ana M. Garcia-Campana, Laura Gamiz-Gracia
Summary: The study investigated the natural occurrence of six major ergot alkaloids in 60 cereal samples from Algeria, revealing that 20% of the samples were contaminated. Wheat was the most contaminated matrix, with ergosine, ergokryptine, and ergocristine showing the highest incidences. Barley had higher concentrations of total ergot alkaloids and was most commonly contaminated with ergometrine.
Article
Chemistry, Analytical
M. Mar Aparicio-Muriana, Rocio Carmona-Molero, Francisco J. Lara, Ana M. Garcia-Campana, Monsalud del Olmo-Iruela
Summary: The development of a HILIC-MS/MS multiclass method for simultaneous analysis of eight cyanotoxins in reservoir water samples is described in this work, providing a reliable and sensitive analytical approach for cyanotoxin analysis. The study shows that a tandem solid-phase extraction system can effectively extract and preconcentrate different classes of cyanotoxins.
Article
Biochemical Research Methods
Laura Carbonell-Rozas, Burkhard Horstkotte, Ana M. Garcia-Campana, Francisco J. Lara
Summary: In this work, an electrophoretic approach based on micellar electrokinetic chromatography coupled with tandem mass spectrometry (MEKC-MS/MS) was proposed for the simultaneous determination of nine neonicotinoids (NNIs) and the fungicide boscalid in pollen and honeybee samples. The method achieved improved separation efficiency and sensitivity through a stacking strategy and a scaled-down QuEChERS sample treatment. The results demonstrated that the proposed method is an environmentally friendly, efficient, sensitive, and useful alternative for the analysis of NNIs and boscalid.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemistry & Molecular Biology
Ivana H. Sramkova, Burkhard Horstkotte, Laura Carbonell-Rozas, Jakub Erben, Jiri Chvojka, Francisco J. Lara, Ana M. Garcia-Campana, Dalibor Satinsky
Summary: Polymeric nano- and microfibers were tested as potential sorbents for the extraction of neonicotinoids from natural waters. Polyimide nanofibers were found to be the most suitable sorbent. Automated preconcentration via online solid-phase extraction and HPLC separation achieved efficient extraction and large preconcentration factors. The developed method showed good performance in terms of analyte recoveries and concentration factors.
Article
Biochemical Research Methods
Laura Carbonell-Rozas, Maykel Hernandez-Mesa, Laura Righetti, Fabrice Monteau, Francisco J. Lara, Laura Gamiz-Gracia, Bruno Le Bizec, Chiara Dall'Asta, Ana M. Garcia-Campana, Gaud Dervilly
Summary: This study evaluates the potential of ion mobility spectrometry (IMS) to improve the analytical performance of liquid chromatography-mass spectrometry (LC-MS) workflows for the determination of ergot alkaloids (EAs) in cereal samples. Collision cross section (CCS) values are used for the unambiguous identification of EA epimers, and they have been validated and compared with predictions from machine-learning models. A LC-travelling wave ion mobility (TWIM)-MS method is developed for the analysis of EAs in barley and wheat samples, resulting in improved signal-to-noise ratio and ion chromatogram quality compared to the LC-TOF-MS method.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Maria del Mar Delgado-Povedano, Francisco J. Lara, Laura Gamiz-Gracia, Ana M. Garcia-Campana
Summary: This study proposes a novel method using non-aqueous capillary electrophoresis (NACE) coupled to quadrupole time-of-flight mass spectrometry (MS) for the identification and quantification of enniatins (ENN) and beauvericin (BEA). The method shows higher selectivity and good recovery and precision in wheat samples. Additionally, two other ENNs were tentatively identified.
Article
Food Science & Technology
Maria del Mar Aparicio-Muriana, Francisco J. Lara, Monsalud Del Olmo-Iruela, Ana M. Garcia-Campana
Summary: The consumption of blue-green algae (BGA) dietary supplements has increased due to their health benefits, but the production of cyanotoxins by cyanobacteria poses serious health risks. This study proposes the use of hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry (HILIC-MS/MS) to detect cyanotoxins in BGA dietary supplements. The method successfully separated and quantified target toxins, including microcystin-leucine-arginine (MC-LR), microcystin-arginine-arginine (MC-RR), nodularin, anatoxin-a, and three non-protein amino acids. The analysis of different commercial supplements revealed the presence of DAB, AEG, and MCs in some samples, emphasizing the importance of monitoring these substances for safe consumption.
Article
Agriculture, Multidisciplinary
Maria Mar Aparicio-Muriana, Renato Bruni, Francisco J. Lara, Monsalud del Olmo-Iruela, Maykel Hernandez-Mesa, Ana M. Garcia-Campana, Chiara Dall'Asta, Laura Righetti
Summary: The increased consumption of blue-green algae (BGA)-based dietary supplements has raised concerns about food safety due to cyanotoxin presence. A study employed ultrahigh-performance liquid chromatography coupled to traveling wave ion mobility spectrometry/quadrupole time-of-flight mass spectrometry (UHPLC-TWIMS-QTOF) to establish a comprehensive database for phycotoxins. Cyanotoxins were confidently identified in commercial BGA dietary supplements based on retention time, m/z, and collision cross section values.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemical Research Methods
Luca Narduzzi, Maria del Mar Delgado-Povedano, Francisco J. Lara, Bruno Le Bizec, Ana Maria Garcia-Campana, Gaud Dervilly, Maykel Hernandez-Mesa
Summary: Cationic, anionic, zwitterionic, and partially polar metabolites are important constituents of blood serum. This study compares two metabolomics-based analytical methods for separating polar compounds in human serum samples, and finds that HILIC-MS is a better tool for polar metabolome analysis.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)