Article
Biochemical Research Methods
N. Fontanals, J. Zohar, F. Borrull, S. Ronka, R. M. Marce
Summary: This study introduces a novel mixed-mode weak cation-exchange (WCX) material prepared by functionalizing a mesoporous divinylbenzene (DVB) resin with maleic acid. The material showed good recovery rates and low matrix effects in the solid-phase extraction (SPE) of basic drugs from environmental water samples. The optimized protocol coupled with high resolution mass spectrometry (HRMS) proved effective in quantifying basic drugs in different environmental water samples.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Multidisciplinary
Hedvika Raabova, Lucie Chocholousova Havlikova, Jakub Erben, Jiri Chvojka, Frantisek Svec, Dalibor Satinsky
Summary: The study introduced the application of a poly-e-caprolactone composite sorbent consisting of micro- and nanometer fibers for the online extraction of non-steroidal anti-inflammatory drugs from a biological matrix, showing successful development of an effective method.
Article
Engineering, Environmental
Changkun Liu, Ye Peng, Chaojie Cai, Jiayan Zhang, Xinzhen Zhao
Summary: This study successfully enhanced the evaporation performance and desalination efficiency of a new type of solar evaporator, while suppressing salt crystallization during the process, providing important insights for the practical application of solar desalination technology.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Biochemical Research Methods
Hongyu Ning, Yilei Fan, Huijun Liu, Zhongping Huang, Xing Ke, Yu Xu, Yuanbin She
Summary: A novel weak cation-exchange sorbent (PS-DVB-WCX-II) was successfully prepared for the selective extraction of illicit drugs in environmental water. The sorbent demonstrated better adsorption performance compared to commercial SPE cartridges and showed high detection sensitivity in real wastewater samples.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Automation & Control Systems
Shuang An, Feiyue Wu, Jie Lian, Dong Wang
Summary: This article investigates the stability problem of discrete-time switched positive linear systems (SPLSs) with marginally stable subsystems. By utilizing the weak common linear copositive Lyapunov function (weak CLCLF) approach, the switching property and the state component property are combined to ensure the asymptotic stability of SPLSs under three types of switching signals. Novel cycle-dependent joint path conditions are proposed for transfer-restricted switching signals, and two types of path conditions are constructed for designing switching schemes under time interval sequence. Additionally, necessary and sufficient conditions for the asymptotic stability of SPLSs under arbitrary switching are established, and three examples are provided to demonstrate the effectiveness of the proposed method.
IEEE TRANSACTIONS ON CYBERNETICS
(2023)
Article
Biochemical Research Methods
Henrique Dipe de Faria, Alberto Thalison Silveira, Beatriz Carvalho do Prado, Julia Louise Moreira Nacif, Mariana Azevedo Rosa, Juliana da Rocha dos Santos, Paulo Caleb Junior Lima Santos, Eduardo Costa Figueiredo, Isarita Martins
Summary: Untreated samples were directly injected into a column switching system, an online solid-phase extraction (SPE) method, using a novel type of restricted access material called restricted access hybrid carbon nanotubes (RAHCNTs), coupled to ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS/MS). RAHCNTs showed improved performance compared to its predecessor (Restricted Access Carbon Nanotubes-RACNTs) for analyzing organic compounds, thanks to their greater adsorption capacity and higher stability in various conditions. The developed method demonstrated high extraction recovery, accuracy, and precision, making it promising for therapeutic monitoring and personalized strategies in antismoking treatment.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Bruna Carneiro Pires, Flavia Viana Avelar Dutra, Hanna Leijoto de Oliveira, Warley de Souza Borges, Keyller Bastos Borges
Summary: By synthesizing a new adsorbent based on restricted access mesoporous magnetic polypyrrole, magnetic solid phase extraction for simultaneous determination of diclofenac, fipronil and febantel in whey samples was successfully achieved. The method showed advantages such as easy operation, high recovery, multiple reuse without losing efficiency, etc.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Melissa Bernhardt, Yiliam Cruz-Garcia, Anne Rech, Svenja Meierjohann, Florian Erhard, Bastian Schilling, Andreas Schlosser
Summary: Mass spectrometry-based immunopeptidomics allows for comprehensive identification of MHC peptides, but their detectability can be influenced by their physicochemical properties. Highly hydrophobic peptides are lost during sample preparation using C18 solid-phase extraction (SPE). To overcome this limitation, an optimized protocol involving restricted access material (RAM) was established, which improved overall MHC peptide recovery and expanded the range of mass spectrometry-detectable MHC peptides to include more hydrophobic peptides.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Apostolia Tsiasioti, Paraskevas D. Tzanavaras
Summary: A new method for the determination of histamine and its precursor histidine in fish products and oriental sauces was developed, validated, and successfully applied for various food samples without matrix interferences. The method showed good precision and recovery rates, with limits of detection of 0.16 and 0.17 mu mol L-1 for histidine and histamine respectively.
Article
Chemistry, Multidisciplinary
Oluseun Akintola, Patrick Gerlach, Christian T. Plass, Andrea Balducci, Winfried Plass
Summary: This study focuses on the characterization and use of the anionic metal-organic framework JUMP-1 and its alkali-metal ion-exchanged analogs as electrode materials for lithium and sodium batteries. The results show that the ion-exchanged materials exhibit higher capacities and better stability compared to the original JUMP-1 compound, indicating their potential as high-performance electrodes for energy storage devices.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Physical
Nafiseh Khodabakhshloo, Bhabananda Biswas
Summary: In this study, modified composites using Iranian zeolite and diatomaceous earth were developed and found to have stronger adsorption capacity for PFOS. The adsorption of PFOS onto the material was mainly due to electrostatic and hydrophobic interactions. This research provides insights into the adhesion mechanisms of PFOS onto untreated and surfactant-altered zeolite and diatomaceous earth in aquatic environments.
APPLIED CLAY SCIENCE
(2023)
Article
Chemistry, Physical
Shengnan Li, Tianyu Liu, Bing Yan
Summary: A multifunctional fluorescent material with ultrahigh stability has been successfully fabricated, which shows excellent performance in detecting 2-methoxyacetic acid and sulfide ions, and the quenching mechanism has been discussed.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Yaoming Wang, Zhengjin Yang, Liang Wu, Liang Ge, Tongwen Xu
Summary: Ion exchange membranes play a crucial role in chemical manufacturing and new applications, improving industrial efficiency and meeting environmental needs. Academician Binglin He has been the most influential figure guiding students into the field of ion exchange membranes. The most promising area for future development is alkaline stable anion-exchange membranes. Scientists driving the development of domestic ion exchange membranes have successfully transferred patented technologies for industrial use.
CHINESE JOURNAL OF CHEMISTRY
(2021)
Article
Biochemical Research Methods
Gaoya Yuan, Xudong Zhang, Yuanyi Zhang, Wanyuan Dong, Yifeng Li
Summary: In this work, a method for continuous separation and collection of acidic and basic charge variants was developed, enabling efficient generation of these variants with high purity.
PROTEIN EXPRESSION AND PURIFICATION
(2023)
Article
Chemistry, Applied
Camille S. Guzella, Denio E. P. Souto, Bruno J. G. Silva
Summary: A novel alginate-based hydrogel device was successfully prepared and applied for solid-phase microextraction of drugs in human plasma, showing high extraction efficiency and precision. This concept has not been explored in previous literature.
CARBOHYDRATE POLYMERS
(2022)
Article
Pharmacology & Pharmacy
Tamaki Miyazaki, Hitomi Kanno, Eiichi Yamamoto, Daisuke Ando, Ken-ich Izutsu, Yukihiro Goda
Article
Chemistry, Medicinal
Yasuhiro Abe, Eiichi Yamamoto, Hiroyuki Yoshida, Akiko Usui, Naomi Tomita, Hitomi Kanno, Sayaka Masada, Hidetomo Yokoo, Genichiro Tsuji, Nahoko Uchiyama, Takashi Hakamatsuka, Yosuke Demizu, Ken-ichi Izutsu, Yukihiro Goda, Haruhiro Okuda
CHEMICAL & PHARMACEUTICAL BULLETIN
(2020)
Article
Pharmacology & Pharmacy
Eiichi Yamamoto, Yuhji Taquahashi, Makiko Kuwagata, Hirokatsu Saito, Kohei Matsushita, Takeshi Toyoda, Futoshi Sato, Satoshi Kitajima, Kumiko Ogawa, Ken-ichi Izutsu, Yoshiro Saito, Yoko Hirabayashi, Yasuo Iimura, Masamitsu Honma, Haruhiro Okuda, Yukihiro Goda
Summary: This study visualized the spatial localization of inhaled ciclesonide and its metabolites in rat lungs using desorption electrospray ionization-time of flight mass spectrometry imaging. The results showed the distribution of ciclesonide and its metabolites in the lungs of rats.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Chemistry, Medicinal
Hidetomo Yokoo, Eiichi Yamamoto, Sayaka Masada, Nahoko Uchiyama, Genichiro Tsuji, Takashi Hakamatsuka, Yosuke Demizu, Ken-ichi Izutsu, Yukihiro Goda
Summary: This study identified specific impurities in ranitidine hydrochloride that can produce the carcinogen NDMA at different rates, with crystalline and amorphous impurities having varying impacts on NDMA formation. Strict control of these impurities can help reduce the risk of NDMA in pharmaceutical products.
CHEMICAL & PHARMACEUTICAL BULLETIN
(2021)
Article
Pharmacology & Pharmacy
Eiichi Yamamoto, Yoshihiro Takeda, Daisuke Ando, Tatsuo Koide, Yuta Amano, Shingo Miyazaki, Tamaki Miyazaki, Ken-ichi Izutsu, Hideko Kanazawa, Yukihiro Goda
Summary: A non-destructive discrimination method using Raman spectroscopy and X-ray CT was developed to differentiate between crystal polymorphs of ranitidine in tablet formulations. X-ray CT imaging revealed varying densities, sizes, and shapes of crystal particles within the same batch, suggesting its potential as a unique method for quantitative physicochemical characterization of crystal polymorphs and solid dosage forms.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Chemistry, Analytical
Seiji Tanaka, Nahoko Uchiyama, Takahiro Goda, Tetsuo Iida, Shinnosuke Horie, Sayaka Masada, Ryoko Arai, Eiichi Yamamoto, Takashi Hakamatsuka, Haruhiro Okuda, Yukihiro Goda
Summary: A simple and rapid method was developed to simultaneously analyze active pharmaceutical ingredients and impurities from metered-dose inhalers using ciclesonide MDI as an example. The method is applicable to other inhalable pharmaceutical products with complicated sample preparation procedures and general pharmaceutical products for profiling impurities.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Instruments & Instrumentation
Daisuke Ando, Tamaki Miyazaki, Eiichi Yamamoto, Tatsuo Koide, Ken-ichi Izutsu
Summary: This study developed a quality evaluation method for dissolving microneedle arrays (DMNAs) and explored the spatial distribution of drugs in DMNAs using Raman spectroscopy mapping. The analysis revealed the crystallization forms of diclofenac sodium (DCF) in the polymer films and the linear relationship between drug content and characteristic peak intensity in the films. Raman imaging demonstrated a homogeneous dispersion of small DCF crystals in the films, as well as the distribution of DCF on the surface of the DMNA needle.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2022)
Article
Biochemical Research Methods
Masaru Kato, Misa Yamaguchi, Tomoka Morita, Noriko Watanabe, Shigenori Ota, Eiichi Yamamoto
Summary: In this study, three types of cationic modified silica monoliths were used to separate and purify nanoparticles, and the nanoparticles were separated from other substances by changing the pH and concentration of the eluent. The results showed that this method can extract nanoparticles in a short time with only low-speed centrifugation and has a high recovery efficiency.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Eiichi Yamamoto, Hitomi Kan-no, Naomi Tomita, Daisuke Ando, Tamaki Miyazaki, Ken-ichi Izutsu
Summary: In order to quantitate NDMA in various drugs, an off-line isolation method was developed and confirmed to be applicable to different medications.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Multidisciplinary
Eiichi Yamamoto, Noriko Tominaga, Hitomi Kan-No, Daisuke Ando, Tamaki Miyazaki, Ken-Ichi Izutsu
Summary: Drug sorption on laboratory materials can affect the performance of analytical methods and lead to unreliable data. By using a linear free energy relationship (LFER) model with Abraham solvation parameters, drug sorption can be characterized and evaluated to prevent it. The physicochemical properties of drugs, rather than the materials used, play a key role in sorption, and additives like organic solvents can alter the rate of drug sorption. Equations have been developed to assess drug sorption extent on glass and polypropylene, and the LFER model is valuable for studying sorption properties in drug discovery research and other biochemical fields.
PHARMACEUTICAL RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Hiroko Shibata, Yusuke Nomura, Tsuyoshi Kawakami, Eiichi Yamamoto, Daisuke Ando, Nahoko Uchiyama, Hiroko Tokumoto, Tatsuo Koide, Hideyuki Sakoda, Hiroyuki Yoshida, Yasuhiro Abe, Takashi Hakamatsuka, Yoshiaki Ikarashi, Yuji Haishima, Akiko Ishii-Watabe, Ken-Ichi Izutsu, Masamitsu Honma, Yukihiro Goda
Summary: Foreign particles were found in Moderna vaccine vials at a vaccination site in Japan, leading to a recall of the affected batches. Analysis indicated that the particles were stainless steel SUS 316L, which had already been administered to some individuals. Further examination with a digital microscope revealed smaller particles, suggesting more vials may have been affected.
YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN
(2022)
Article
Nanoscience & Nanotechnology
Eiichi Yamamoto, Naoki Hosogi, Yuki Takechi-Haraya, Ken-ichi Izutsu, Nahoko Uchiyama, Yukihiro Goda
Summary: High-resolution cryogenic transmission electron microscopy (cryo-TEM) revealed that doxorubicin sulfate crystals in liposomes (prepared by remote loading with ammonium sulfate) formed folded, undulating, and fibrous structures with a diameter of approximately 2.4 nm. An undulating, fibrous crystal considered to be undergrowth, as well as bundles of fibrous crystals, were observed in doxorubicin-loaded liposomes. This explains the formation of various shaped doxorubicin sulfate crystals, such as curved, U-shaped, or circular, in addition to the cylinder and/or rod-like crystals reported in the literature. Liposomes without crystals contained high-density aqueous phases, indicating that doxorubicin is loaded remotely and remains as a solute without precipitation.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2023)
Article
Biochemical Research Methods
Yusuke Tamai, Akira Noda, Eiichi Yamamoto
Summary: The challenge of controlling analogous substances in complex drug candidates has increased, especially with the rise of candidates that do not comply with Lipinski's rule of five. Although advancements in liquid chromatography have improved efficiency, difficulties in separating similar impurities with high-performance liquid chromatography (HPLC) still remain. Bayesian inference is applied to develop an algorithm that assigns confidence intervals to quantitative data of each analogous substance using the MCR-ALS method.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Yusuke Nakai, Akira Noda, Eiichi Yamamoto
Summary: The stability of APIs and formulations is crucial in the pharmaceutical industry, affecting research and development feasibility, development period, and costs. To predict early-stage formulation stability accurately, we developed an algorithm combining Bayesian inference and the conventional Arrhenius equation, providing a narrow confidence interval even with data variations. Our method enables the reasonable prediction of long-term drug stability and contributes to pharmaceutical product development worldwide.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Pharmacology & Pharmacy
Hiroko Tokumoto, Eiichi Yamamoto, Takashi Hakamatsuka, Nahoko Uchiyama
Summary: The use of HRXCT technology allows for easy observation of the internal morphology of crude drugs. Compared to microscopic examination, HRXCT can observe the same internal morphological characteristics and simplifies the technical steps.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2022)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
