期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1204, 期 1, 页码 110-113出版社
ELSEVIER
DOI: 10.1016/j.chroma.2008.07.050
关键词
CLA; free fatty acid; ruminal liquid; sulphuric acid; methylation; silver-ion HPLC; GC
资金
- Ministerio de Ciencia e Innovacion [AGL2005-04760-C02-01/ALI, CSD2007-063]
- Comunidad Autonoma de Madrid [S-0505/AGR/000153]
This study used GC and silver-ion HPLC to examine the effects of temperature and time on methylation of individual and mixtures of conjugated linoleic acid (CLA) isomers in free fatty acid form using sulphuric acid as catalyst. In the conditions tested (temperatures between 20 and 50 degrees C and times between 10 and 60 min) methylation was complete while avoiding isomerization of conjugated dienes and the formation of artefacts that could interfere with chromatographic determinations. An analytical method using solvent extraction of the lipids followed by selective elution of the free fatty acids from aminopropyl bonded phase columns and methylation with H2SO4 in mild conditions was then applied to determine the CLA isomers in free fatty acid form in rumen fluid, and the results were evaluated. (c) 2008 Elsevier B.V. All rights reserved.
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