Article
Biochemistry & Molecular Biology
Shuang Guo, Dongyu Gu, Chang Liu, Shanshan Tang, Yi Wang, Yi Yang
Summary: This study explored the antioxidant effect and mechanism of scavenging free radicals of Morus alba leaves, and found that rutin is the main antioxidant in Morus alba leaves. The study also revealed that the 4'-OH group in rutin scavenges free radicals through hydrogen atom transfer in non-polar media and single electron transfer followed by proton transfer in polar media.
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
(2023)
Article
Chemistry, Analytical
Xiaowei Dong, Hongping Huang, Rong Wang, Shiyu Luo, Yahui Mi, Yuqing Pan, Wei Shen, Jiamin Cui, Xiaolong Hu, Xuexiang Cheng, Xinhong Shi, Hao Wang
Summary: In this study, phenolic compounds were successfully separated from Chrysanthemum morifolium cv. Fubaiju flowers, including flavonoid glycosides and quinic acid derivatives. The isolation was achieved by using high-speed counter-current chromatography and preparative high-performance liquid chromatography. The isolated compounds were evaluated for their protective effects on H2O2-induced oxidative damage in adult retinal pigment epithelial cells.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Food Science & Technology
Xiao Li, Jing-Nan Ren, Gang Fan, Shu-Zhen Yang, Lu-Lu Zhang, Si-Yi Pan
Summary: Nootkatone, an important functional sesquiterpene, was successfully separated and purified from the fermentation broth by using high-speed counter-current chromatography (HSCCC). The selected solvent system proved to be effective in separating the target product, yielding nootkatone with high purity.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2022)
Article
Biotechnology & Applied Microbiology
Joao P. Mendes, Ricardo J. S. Silva, Mikael Berg, Linda Mathiasson, Cristina Peixoto, Paula M. Alves, Manuel J. T. Carrondo
Summary: Virus-based biologicals, particularly oncolytic viruses, have promising applications in the field of medicine. The use of periodic counter-current chromatography (PCC) has shown to be an efficient method for intermediate purification of oncolytic adenovirus, leading to high virus recoveries and impurity reductions. The study also highlights the importance of process stability and automation in ion-exchange chromatography capture.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Chemistry, Analytical
Zhibo Song, Tao Chen, Shuo Wang, Cheng Shen, Hongmei Li, Aijing Li, Peipei Li, Yumei Ma, Zhi Chen, Yulin Li
Summary: In this study, an efficient strategy for the large-scale preparation of two minor polar polyphenols from Phyllanthus emblica L. has been established using macroporous resin column chromatography and high-temperature preparative high-speed counter-current chromatography. The combination of enrichment and separation steps provides a reliable method for obtaining compounds with high purity.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Biochemical Research Methods
Mateusz Dembowski, John E. Rowley, Kevin Boland, Janelle Droessler, David A. Hathcoat, Alexandria Marchi, George S. Goff, Iain May
Summary: This study evaluates the application of High-Speed Counter-Current Chromatography (HSCCC) in the separation of non-radioactive rare earth elements (REEs). The results demonstrate the feasibility and effectiveness of HSCCC in separating a subset of REEs, providing potential applications in radioelement separation chemistry.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Nuanyi Liang, Lucie Necasova, Yuan-Yuan Zhao, Jonathan M. Curtis
Summary: This study describes an analytical-semipreparative scale counter-current chromatography (CCC) method for enriching multiple ganglioside homologues, demonstrating successful separation of porcine gangliosides using adjustments in solvent system composition and elution modes.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Review
Food Science & Technology
Lingxi Li, Jian Zhao, Tingting Yang, Baoshan Sun
Summary: Polyphenols, the most abundant dietary antioxidants found in fruits, vegetables, cereals, and legumes, have shown the ability to prevent various diseases. Conventional separation techniques for polyphenols are complicated and expensive, while high-speed countercurrent chromatography (HSCCC) offers an efficient and cost-effective method for large-scale separation and purification of polyphenols.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Chemistry, Analytical
Sha Li, Tiandan Li, Xiaochao Hu, Yong Yang, Yangyi Huang, Kai He
Summary: This study demonstrated the importance of the retention of the stationary phase in achieving high-resolution separation in HSCC. By investigating the correlation between settling time and retention of the stationary phase in different solvent systems, the study provides valuable insights for solvent system selection in HSCCC.
Article
Pharmacology & Pharmacy
Joao P. Mendes, Magnus Bergman, Anita Solbrand, Cristina Peixoto, Manuel J. T. Carrondo, Ricardo J. S. Silva
Summary: Replacing batch unit operations of biopharmaceuticals by continuous manufacturing with periodic counter-current chromatography (PCC) is a mature concept. This study describes the implementation of continuous adeno-associated virus (AAV) affinity capture using a four-column PCC process, which achieved nearly threefold improvement in productivity compared to the optimized batch process.
Article
Chemistry, Analytical
Raja Ghosh
Summary: This paper discusses the use of an in-house designed membrane chromatography device for ultrahigh-speed, ultrahigh-resolution preparative protein separation. The experiments show that membrane chromatography has higher resolution and faster speed compared to resin-packed preparative columns, making it a better option for protein purification. Membrane chromatography is also effective in resolving tracer impurity peaks.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Yifeng Zhou, Meixu Chen, Xinyi Huo, Qilin Xu, Linlin Wu, Liling Wang
Summary: Flavonoids, including isoorientin, orientin, vitexin, and isovitexin, were successfully extracted and enriched from bamboo leaves extraction residues using macroporous resins. The XAD-7HP resin showed the best adsorption and desorption performance and was selected for further evaluation. Dynamic resin column chromatography achieved a 4.5-fold increase in the content of the four flavonoids, with recoveries ranging from 72.86% to 88.21%. In addition, chlorogenic acid with a purity of 95.1% was obtained during the separation process. This method provides a rapid and efficient way to utilize bamboo leaves extraction residues for the production of high-value-added food and pharmaceutical products.
Article
Biochemical Research Methods
Haoquan Li, Zengyuan Cao, Shuang Guo, Dongyu Gu, Shuai Wang, Yi Yang, Fei He
Summary: In this study, a method based on HSCCC was successfully established to separate rutin from Apocynum venetum, resulting in a high purity rutin extract. Consecutive HSCCC was introduced to further improve the yield, providing a strategy not only for the scale-up production of rutin but also for other natural active compounds.
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
(2021)
Article
Chemistry, Analytical
Xiaohan Zhu, Pengcheng Li, Jintian Tang, Yanqi Su, Mi Xiao, Hongkun Xue, Xu Cai
Summary: The selection of an appropriate solvent system is crucial in high-speed countercurrent chromatography (HSCCC) separation. The polarity of compounds plays an important role in HPLC analysis and HSCCC separation, and can be calculated using the HPLC polarity parameter model and the average polarity of the HSCCC solvent system. This study validated the applicability and accuracy of the HPLC polarity parameter model and selected suitable HSCCC solvent systems for separation experiments.
ANALYTICAL METHODS
(2022)
Article
Plant Sciences
Chen Xiang-Yang, Bi Shu-Feng
Summary: The volatile components of Zephyranthes candida flowers were extracted and analyzed for the first time using headspace-solid phase micro-extraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS). The major compounds identified in the volatile extract were 1-hexanol, methyl heptanoate, hexyl acetate, 2-acetyl pyrrole, methyl-alpha-D-rhamnopyranoside, phenethyl alcohol, heptanoic acid, and 1-octanol.
BANGLADESH JOURNAL OF BOTANY
(2022)