Article
Biochemical Research Methods
C. Soto, H. D. Ponce-Rodriguez, J. Verdu-Andres, R. Herraez-Hernandez, P. Campins-Falco
Summary: In this study, three miniaturized liquid chromatography instruments were evaluated for analyzing caffeine in dietary supplements. The benchtop systems showed superior sensitivity, with capillary LC and nano LC systems having lower limits of detection compared to the portable LC system. The portable LC method was simpler and faster, while still providing accurate quantification of caffeine content in the samples.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Food Science & Technology
Oktawia Kalisz, Sylwia Studzinska, Szymon Bocian
Summary: Caffeine is a natural psychoactive substance found in various plants such as coffee, tea, cocoa, guarana, and yerba mate. It is commonly added to dietary supplements for its metabolism-boosting and weight loss benefits. A novel UHPLC method using environmentally friendly solvents was developed to determine the caffeine content in dietary supplements.
Article
Biochemistry & Molecular Biology
Camila Soto, Henry Daniel Ponce-Rodriguez, Jorge Verdu-Andres, Pilar Campins-Falco, Rosa Herraez-Hernandez
Summary: With the growth of the dietary supplements industry, there is a demand for simplified and easy-to-use instruments. This study tested a hand-portable miniaturized liquid chromatograph for the determination of chlorogenic acids. The results showed that the portable LC instrument had comparable analytical performance and faster speed for quantification.
Article
Agriculture, Multidisciplinary
Natalia Arroyo-Manzanares, Vicente Rodriguez-Estevez, Ana M. Garcia-Campana, Elena Castellon-Rendon, Laura Gamiz-Gracia
Summary: The occurrence of ergot alkaloids in feed samples in Spain appears to be lower than in other regions of Europe. All samples met current recommendations of the feed industry regarding practical limits for ergot alkaloids in pig feeds.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Chemistry, Applied
Delia Castilla-Fernandez, David Moreno-Gonzalez, Juan F. Garcia-Reyes, Evaristo Ballesteros, Antonio Molina-Diaz
Summary: Datura species are known for their high concentration of tropane alkaloids, which can lead to poisoning when accidentally mixed with edible crops. Testing on frozen spinach and spinach-based infant food products found that some samples had levels close to the maximum limits set by the European Union. This highlights the global concern over the presence of these compounds in various commodities.
Article
Biochemical Research Methods
Lisa Monika Klein, Angelika Miriam Gabler, Michael Rychlik, Christoph Gottschalk, Florian Kaltner
Summary: This study developed a sensitive analytical approach for simultaneous detection and quantification of unsaturated pyrrolizidine alkaloids, their corresponding N-oxides, and tropane alkaloids in milk of dairy cows. The method showed low limits of detection and quantification, good recovery rates and repeatability. Traces of pyrrolizidine alkaloids were detected in three milk samples obtained from milk vending stations and local marketers in Bavaria, Germany.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Henry Daniel Ponce-Rodriguez, Jorge Verdu-Andres, Pilar Campins-Falco, Rosa Herraez-Hernandez
Summary: This study used capillary liquid chromatography with diode array detection to determine different terpenes in dietary supplements, proposing an innovative method capable of detecting target compounds at concentration levels as low as 0.006 mg per gram of sample with a total analysis time <30 min. The reliability of the method was confirmed through analyzing various botanical dietary supplements.
Article
Food Science & Technology
Alejandro Garcia-Juan, Nuria Leon, Sergio Armenta, Olga Pardo
Summary: This study successfully developed and validated an analytical method for the simultaneous determination of ergot, tropane, and pyrrolizidine alkaloids in cereal-based food. The method showed high recoveries and precision, meeting the validation requirements. Analysis of reference materials further confirmed the reliability of the method. Analysis of actual samples revealed the presence of ergot alkaloids and pyrrolizidine alkaloids in some samples.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Applied
Donatella Nardiello, Wadir M. V. Marchesiello, Stefano Sportelli, Lucia Bonassisa, Donghao Li, Maurizio Quinto
Summary: A rapid LC/tandem MS method was proposed for the quantitative determination of matrine-based biopesticides in fruit and vegetable samples. The method achieved excellent chromatographic results in less than 10 minutes, with good selectivity, linearity, and detection limits. It was successfully applied to the analysis of commercially available fruit and vegetable samples.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Chemistry, Analytical
Ensie Hosseini, Jahan B. Ghasemi, Maryam Shekarchi
Summary: The study developed an analytical method for the simultaneous detection and quantitative determination of three key adulterants in dietary food supplements. The proposed method benefits from optimization and calibration, yielding reliable results.
JOURNAL OF AOAC INTERNATIONAL
(2022)
Article
Engineering, Chemical
Feifei Zhong, Li Ouyang, Nan Deng, Fangping Yin, Jiajie He, Deqing Lei, Jieying Gao, Hui Zeng, Zhaoxia Wang, Lu Wang, Lixia Yang, Hui Zhou
Summary: In this study, a method for the simultaneous determination of seven alkaloids in Semen Nelumbinis and its products was established. The extraction process and HPLC method were optimized using a single factor experiment. The results showed that the method had good linearity and could accurately quantify the alkaloids in the samples. Lotusine and Neferine were found to be the most abundant alkaloids in the samples.
Article
Biochemical Research Methods
Nuria Leon, Pablo Miralles, Vicent Yusa, Clara Coscolla
Summary: A green analytical method has been developed to determine the levels of 30 tropane and pyrrolizidine alkaloids and their N-oxides in dried teas and herbs. The method combines the QuEChERS procedure with LC-Q-Orbitrap HRMS analysis. The method has been validated and shown to have satisfactory performance in different tea and herb matrices. The results also demonstrate that the analyzed tea and herb samples comply with European regulations.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Food Science & Technology
Sandra Barreiro Astray, Letricia Barbosa-Pereira, Maria Asuncion Lage-Yusty, Julia Lopez-Hernandez
Summary: The study comparing two methods for determining melatonin in food supplements showed that constant-wavelength synchronous spectrofluorimetry is a fast, simple, and economical method for rapid and accurate determination of a single compound, while the HPLC-VWD-FLD method is more suitable for the determination of specified compounds in complex samples due to its high sensitivity and separation efficiency.
FOOD ANALYTICAL METHODS
(2021)
Article
Agriculture, Multidisciplinary
Gema Casado-Hidalgo, Gonzalo Martinez-Garcia, Sonia Morante-Zarcero, Damian Perez-Quintanilla, Isabel Sierra
Summary: An efficient and simple method was developed and validated for the analysis of six opium alkaloids in bakery products. The method showed adequate recoveries, low standard deviations, and no matrix effects. The study also revealed that some bakery products contained opium alkaloids exceeding the regulated standard.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Analytical
Xiaomei Chen, Liulin Wei, Qian Liu, Jingchan Zhao
Summary: A novel method utilizing SLSC-ME-HPLC-UV was developed for preconcentrating trace bisphenols in environmental water, achieving high extraction efficiency through optimized conditions.
MICROCHEMICAL JOURNAL
(2021)
