Article
Infectious Diseases
Martina Cremanns, Felix Lange, Soeren G. Gatermann, Niels Pfennigwerth
Summary: The study identified resistance mechanisms in Klebsiella pneumoniae with varying levels of resistance to carbapenems, mainly through mutations in ompK36 and the rpoE gene. These mutations affect bacterial resistance and growth rates, reducing the expression of major outer membrane proteins. Enhanced synthesis of sigma(E) leads to increased resistance to cephalosporins and carbapenems in clinical K. pneumoniae isolates, accompanied by a significant reduction in growth rates.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Brian D. Johnston, Paul Thuras, Stephen B. Porter, Melissa Anacker, Brittany VonBank, Paula Snippes Vagnone, Medora Witwer, Mariana Castanheira, James R. Johnson
Summary: The study characterized a globally distributed collection of carbapenem-resistant Escherichia coli isolates and identified diverse molecular traits and antibiotic resistance patterns among different geographical regions. The global population of CR E. coli is highly diverse and varies significantly by geographical region, presenting challenges for prevention and management that require ongoing surveillance.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Microbiology
Junxin Zhou, Weiping Wang, Min Liang, Qian Yu, Shiqi Cai, Tailong Lei, Yan Jiang, Xiaoxing Du, Zhihui Zhou, Yunsong Yu
Summary: The study aimed to investigate the molecular mechanism underlying ceftazidime-avibactam resistance caused by a novel CMY-178 variant. The results showed that CMY-178 increased the resistance to multiple cephalosporins and ceftazidime-avibactam compared with CMY-172. Structural modeling and molecular docking revealed that CMY-178 had a narrower ligand-binding pocket and a stronger positive charge at the pocket entrance than CMY-172. The bla(CMY-178) gene was located in a transferable plasmid with high transfer frequency and no fitness costs.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Tam Tran, Sylvia Checkley, Niamh Caffrey, Chunu Mainali, Sheryl Gow, Agnes Agunos, Karen Liljebjelke
Summary: This study identified potential horizontal gene transfer events of antimicrobial resistance genes between E. coli and Salmonella in broiler chicken production, revealing various resistant genes in the process.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Engineering, Multidisciplinary
Yingbo Shen, Fupin Hu, Yongqiang Wang, Dandan Yin, Lu Yang, Yiqiang Chen, Chunyan Xu, Jiyun Li, Junyao Jiang, Xueyang Wang, Yulin Fu, Dongyan Shao, Dejun Liu, Tengfei Ma, Chang Cai, Zhangqi Shen, Shaolin Wang, Juan Li, Rong Zhang, Yuebin Ke, Congming Wu, Jianzhong Shen, Timothy R. Walsh, Yang Wang
Summary: Although carbapenem use is prohibited in animals in China, carbapenem-resistant Escherichia coli (CREC), especially New Delhi metallo-b-lactamase (NDM)-producing strains, are widely prevalent in food-producing animals. A study found that being male, an age below 1, an age between 13 and 18, provinces with greater chicken production, and provinces with higher pig production were associated with higher odds of CREC infection. The study also revealed frequent transmission of CREC between humans and animals.
Article
Chemistry, Multidisciplinary
Catherine L. Tooke, Philip Hinchliffe, Michael Beer, Kirill Zinovjev, Charlotte K. Colenso, Christopher J. Schofield, Adrian J. Mulholland, James Spencer
Summary: KPC-2 is a carbapenemase that confers resistance to β-lactam antibiotics in Gram-negative pathogens. The flexible a-loop in KPC-2 plays a role in catalytic activity, while carbapenemase activity is mainly due to efficient deacylation of the A2-enamine acyl-enzyme tautomer. Quantum mechanics/molecular mechanics simulations reveal the specific reactivity of the two isomers.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Microbiology
Franca Schaefer, Pia Goerner, Sabrina Woltemate, Christina Brandenberger, Robert Geffers, Stefan Ziesing, Dirk Schlueter, Marius Vital
Summary: This study investigated the physiological adaptation of carbapenem-resistant Enterobacteriaceae isolates with different resistance mechanisms during growth with sublethal concentrations of ertapenem. The results showed distinct strategies and gene expression patterns between strains carrying carbapenemase or alterations in porin-encoding genes. The study also observed a survival-like phenotype and reduced growth efficiencies in strains with alterations in porin-encoding genes, indicating costs of resistance.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Zoi Athanasakopoulou, Katerina Tsilipounidaki, Marina Sofia, Dimitris C. Chatzopoulos, Alexios Giannakopoulos, Ioannis Karakousis, Vassilios Giannakis, Vassiliki Spyrou, Antonia Touloudi, Maria Satra, Dimitrios Galamatis, Vassilis Diamantopoulos, Spyridoula Mpellou, Efthymia Petinaki, Charalambos Billinis
Summary: This study identified 13 Escherichia coli strains producing plasmid-encoded AmpC beta-lactamases (pAmpC) in fecal samples from animals in Greece, with bla(CMY-2) being the predominant gene. The strains belonged to different sequence types and displayed co-resistance to various antibiotics, including tetracyclines and sulfonamides, along with mutations in quinolone resistance-determining regions. This report provides evidence of pAmpC dissemination and expands the knowledge on antibiotic resistance in animals in Greece.
Article
Infectious Diseases
Sien De Koster, Moniek Ringenier, Christine Lammens, Arjan Stegeman, Tijs Tobias, Francisca Velkers, Hans Vernooij, Marjolein Kluytmans-van den Bergh, Jan Kluytmans, Jeroen Dewulf, Herman Goossens
Summary: This study found marked differences in antibiotic resistance between countries, farms, and within farms in Belgium and the Netherlands. ESBL-E. coli were more prevalent in Belgian samples, while CiproR-E. coli were highly prevalent in broilers of both countries. Multidrug resistance was frequently observed in samples from both countries, with a higher prevalence of ESBL production in combination with ciprofloxacin resistance in samples from Belgium.
Article
Microbiology
Somorita Baishya, Chandrayee Deshamukhya, Jayalaxmi Wangkheimayum, Bhaskar Jyoti Das, Anand Anbarasu, Anupam Das Talukdar, Amitabha Bhattacharjee, Manabendra Dutta Choudhury
Summary: The study identified secondary resistance genes repA2 and ccdB as assisting in the maintenance of primary resistance genes bla(NDM) and bla(CTX-M) under carbapenem and cephalosporin stress, potentially leading to the reversion of antibiotic susceptibility in resistant bacteria.
Article
Multidisciplinary Sciences
Parichart Boueroy, Thidathip Wongsurawat, Piroon Jenjaroenpun, Peechanika Chopjitt, Rujirat Hatrongjit, Sathaporn Jittapalapong, Anusak Kerdsin
Summary: This study investigated the genetic features of mcr-1-carrying plasmids in bacteria and found that diverse promiscuous plasmids may facilitate the spread of the mcr-1 gene. These results provide important information for surveillance systems and infection control.
SCIENTIFIC REPORTS
(2022)
Article
Biology
Qingqing Fang, Yu Feng, Alan McNally, Zhiyong Zong
Summary: Fang et al. identified two previously unidentified phages that can inhibit the growth and decrease the virulence of carbapenem-resistant Klebsiella pneumoniae (CRKP). They also found that CRKP can develop phage resistance but can still be eliminated in a mouse intestinal colonization model, highlighting the potential of phage therapy as a treatment against drug-resistant pathogens.
COMMUNICATIONS BIOLOGY
(2022)
Article
Microbiology
Fengtian Li, Kun Ye, Xin Li, Liyan Ye, Ling Guo, Lifeng Wang, Jiyong Yang
Summary: This study investigated 144 carbapenem-resistant Escherichia coli (CREco) isolates from 10 cities in China, finding that the majority belonged to ST167, followed by ST410 and ST131; most of them carried various NDM-coding genes, with some ST types showing an outbreak trend.
Article
Cell Biology
Xian-liang Zhao, Zhuang-gui Chen, Tian-ci Yang, Ming Jiang, Jie Wang, Zhi-xue Cheng, Man-jun Yang, Jia-xin Zhu, Tian-tuo Zhang, Hui Li, Bo Peng, Xuan-xian Peng
Summary: Exogenous glutamine can enhance the killing effect of antibiotics against multidrug-resistant bacteria, promote the influx of antibiotics, increase intracellular antibiotic concentrations, effectively prevent the development of antibiotic resistance, and provide new insights for the prevention or management of chronic, multidrug-resistant bacterial infections, bacterial persistence, and difficult-to-treat bacterial biofilms.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Microbiology
Live L. Nesse, Solveig S. Mo, Silje N. Ramstad, Ingun L. Witso, Camilla Sekse, Anna Eline E. Bruvoll, Anne Margrete Urdahl, Lene K. Vestby
Summary: Norwegian monitoring program detected Extended-spectrum cephalosporin-resistant Escherichia coli (ESCR E. coli) with plasmids carrying the bla(CMY-2) resistance gene in the Norwegian broiler production chain, which displayed relatively low biofilm forming abilities. The study found that these plasmids may cause the observed reduction in biofilm formation through changes in flagellar expression or function.
Article
Medicine, General & Internal
Ming-Shyan Lin, Huang-Shen Lin, Chang-Ming Chung, Yu-Sheng Lin, Mei-Yen Chen, Po-Han Chen, Jing-Hong Hu, Wen-Nan Chou, Jui-Chu Huang, Tung-Jung Huang
Article
Infectious Diseases
Huang-Shen Lin, Ming-Hsun Lee, Chun-Wen Cheng, Po-Chang Hsu, Hsieh-Shong Leu, Ching-Tai Huang, Jung-Jr Ye
INFECTIOUS DISEASES
(2015)
Article
Infectious Diseases
Jung-Jr Ye, Huang-Shen Lin, Chun-Fu Yeh, Yen-Mu Wu, Po-Yen Huang, Chien-Chang Yang, Ching-Tai Huang, Ming-Hsun Lee
BMC INFECTIOUS DISEASES
(2016)
Article
Medicine, General & Internal
Jing-Hong Hu, Mei-Yen Chen, Chau-Ting Yeh, Huang-Shen Lin, Ming-Shyan Lin, Tung-Jung Huang, Ming-Ling Chang
Article
Endocrinology & Metabolism
Ming-Shyan Lin, Su-Er Guo, Huang-Shen Lin, Jen-Te Hsu, Yu-Sheng Lin, Tsai-Hui Lin, Tung-Jung Huang, Mei-Yen Chen, Chang-Min Chung
Article
Infectious Diseases
Jung-Jr Ye, Huang-Shen Lin, An-Jing Kuo, Hsieh-Shong Leu, Ping-Cherng Chiang, Ching-Tai Huang, Ming-Hsun Lee
JOURNAL OF INFECTION
(2011)
Article
Medicine, General & Internal
Ming-Shyan Lin, Po-Han Chen, Po-Chang Wang, Huang-Shen Lin, Tung-Jung Huang, Shih-Tai Chang, Wen-Nan Chiu, Mei-Yen Chen
Article
Clinical Neurology
Kuo-Hsuan Chang, Rong-Kuo Lyu, Wan-Ting Lin, Yu-Tung Huang, Huang-Shen Lin, Shang-Hung Chang
FRONTIERS IN NEUROLOGY
(2019)
Article
Immunology
Chun-Wen Cheng, Huang-Shen Lin, Jung-Jr Ye, Chien-Chang Yang, Ping-Cherng Chiang, Ting-Shu Wu, Ming-Hsun Lee
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2009)
Article
Immunology
Huang-Shen Lin, Jung-Jr Ye, Tsung-Yu Huang, Po-Yen Huang, Ting-Shu Wu, Ming-Hsun Lee
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2008)
