Article
Environmental Sciences
Lenka Luptakova, Simona Dvorcakova, Zuzana Demcisakova, Lassaad Belbahri, Katarina Holovska, Eva Petrovova
Summary: The study revealed that DMSO has embryotoxic effects on developing chicken embryos, sometimes resulting in malformations. At the molecular level, DMSO affects the expression of CYP genes. Additionally, increasing doses of DMSO lead to higher embryo mortality rates.
Article
Multidisciplinary Sciences
Lulu Ni, Ping Miao, Jian Jiang, Fang Wan, Jiangan Li, Min Ai, Lingzhong Kong, Su Tu
Summary: This study found that Glycyrrhiza promotes the metabolism of toxic components of Aconitum by upregulating the expression of CYP3A, reducing the content of heart failure-related markers, and improving the myocardial energy metabolism disorder, thus alleviating the development of congestive heart failure.
Article
Chemistry, Inorganic & Nuclear
Lu-Lu Liu, Li-Xin Wang, Min Peng, Jing Xiang, Huan Yang, Shek-Man Yiu, Tai-Chu Lau
Summary: The reactivity of electrophilic OsVI and RuVI nitrides with amines has been reported before. In this study, a luminescent osmium(VI) nitrido complex was found to undergo ring nitrogenation of aromatic amines under visible light irradiation. The reaction proceeds through nucleophilic addition of the amine nitrogen to the excited state complex. The products were characterized using spectroscopic techniques and X-ray crystallography. The reaction mechanism involves electron transfer and proton transfer steps.
INORGANIC CHEMISTRY
(2023)
Article
Chemistry, Inorganic & Nuclear
Shaoru Chen, Jieying Hu, Hua-Qun Zhou, Fangying Yu, Can-Min Wu, Lai-Hon Chung, Lin Yu, Jun He
Summary: Three primitive metal-organic frameworks bearing triazine-centered linkers were prepared and studied for their electrocatalytic hydrogen evolution reaction (HER) performance. Transition metal loading was found to enhance the electrocatalytic activity, with ZrL3TM demonstrating the best HER performance. This work highlights the effective strategy of microenvironment regulation to improve the electrocatalytic activity of MOF materials.
INORGANIC CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Peng Jiang, Hongbo Jin, Guangtao Zhang, Wenjun Zhang, Wei Liu, Yiguang Zhu, Changsheng Zhang, Liping Zhang
Summary: This study provides mechanistic understanding of the functional differences between the homologous cytochrome P450 enzymes IkaD and CftA, which catalyze multiple C-H oxidations on the same substrate with distinct regio- and chemoselectivity. The crystal structures reveal that the differences in the conformation of the F/G region in IkaD and CftA control the substrate orientation and determine their binding patterns with polar moieties. Fine-tuning the polar subpocket of IkaD can alter its regioselectivity.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Gastroenterology & Hepatology
Fubo Ji, Jianjuan Zhang, Niya Liu, Yuanzhuo Gu, Yan Zhang, Peipei Huang, Nachuan Zhang, Shengda Lin, Ran Pan, Zhuoxian Meng, Xin-Hua Feng, Stephanie Roessler, Xin Zheng, Junfang Ji
Summary: This study identified sex-related molecules and their associated functions in hepatocellular carcinoma (HCC) patients and found that the liver-specific gene CYP39A1 was significantly suppressed in HCC tumors. The study also showed that overexpression of CYP39A1 inhibited tumor formation in both female and male mice, suggesting that strategies to up-regulate CYP39A1 could be a promising treatment for HCC in both genders.
Article
Multidisciplinary Sciences
Wanfu Wu, Margaret Warner, Li Wang, Wei-Wei He, Ruipeng Zhao, Xiaoxiang Guan, Cindy Botero, Bo Huang, Charlotte Ion, Charles Coombes, Jan-Ake Gustafsson
Summary: Comparing gene expression profiles of malignant parts and normal adjacent parts of TNBC breasts revealed that CYP-mediated pathways may drive TNBC. However, despite being expressed in TNBC, ER13 is unlikely to be a tumor suppressor as its absence of main tethering partners renders it ineffective on genes related to proliferation and inflammation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biotechnology & Applied Microbiology
Sheng Dong, Jingfei Chen, Xingwang Zhang, Fei Guo, Li Ma, Cai You, Xiao Wang, Wei Zhang, Xiaobo Wan, Shuang-Jiang Liu, Li-Shan Yao, Shengying Li, Lei Du, Yingang Feng
Summary: Selective oxidation of C-H bonds in alkylphenols is crucial for structural derivatization in pharmaceutical and biomanufacturing, as well as for biological degradation of toxic chemicals in environmental protection. A novel chemomimetic biocatalytic system using cytochrome P450 monooxygenase CreJ has been developed, showing excellent regio- and stereoselectivity in oxidizing various alkylphenol substrates. The results provide mechanistic insights into CreJ regio- and stereoselectivity, offering promising directions for further enzyme design and engineering efforts.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Editorial Material
Biochemistry & Molecular Biology
F. Peter Guengerich
Summary: The paper introduces the concept of cytochrome b(5) as an auxiliary protein facilitating cytochrome P450 reactions, providing important insights for future research.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Multidisciplinary Sciences
Laurentijn Tilleman, Kaat Rubben, Wim Van Criekinge, Dieter Deforce, Filip Van Nieuwerburgh
Summary: This study tested the effectiveness of Targeted Locus Amplification (TLA) combined with Illumina or Nanopore sequencing in accurate genotyping and phasing of pharmacogenes. The results showed limited nucleotide mapping on target and incomplete coverage of genes with both sequencing methods. However, the optimized primer pair for the CYP2D6 gene in the Nanopore dataset resulted in correct haplotypes, indicating the potential reliability of this method with patient-specific primer design and optimization.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Freeborn Rwere, Sangchoul Im, Lucy Waskell
Summary: By altering the 140s FMN binding loop of CYPOR, the activity with cyt P450s can be affected, with the mutant activity primarily determined by its ability to reduce ferric cyt P450. The different behaviors of the mutants are mainly due to the altered structure and mobility of the loop.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Rui Xiong, Ting He, Yamei Wang, Shifan Liu, Yameng Gao, Hanwei Yan, Yan Xiang
Summary: This study identified 188 PnCYP genes, most of which underwent purification selection and continuous replication during evolution; GO analysis revealed functional diversity of these genes; functional divergence analysis at the amino acid level identified critical sites. After nitrogen treatment, 10 genes were significantly up-regulated, 37 genes were significantly down-regulated, and five potential functional genes responded to MEJA and ABA treatment.
Article
Engineering, Environmental
Toshio Mori, Haruka Ohno, Hirofumi Ichinose, Hirokazu Kawagishi, Hirofumi Hirai
Summary: In this study, it was found that the white-rot fungus P. chrysosporium degrades neonicotinoid insecticides through the activity of at least two different CYP isozymes.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Yanhui Lu, Qi Bai, Qiang Li, Xusong Zheng, Junce Tian, Jiawen Guo, Hongxing Xu, Zhongxian Lu
Summary: The study identified and characterized two cytochrome P450 monooxygenase genes, CsCYP6SN3 and CsCYP306A1, associated with the lethal effect of vetiver against C. suppressalis larvae. Inhibition of these genes significantly reduced pupation rate and pupa weight in the pests.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Inorganic & Nuclear
Yunling Pan, Lin Cheng, Yi Pan, Wai-Lun Man, Shek-Man Yiu, Jianhui Xie, Kai-Chung Lau, Tai-Chu Lau
Summary: This study reports the first example of oxidative cleavage of primary amines by a ruthenium(VI) nitrido complex. The reaction is driven by the formation of stable NuN after the initial coupling of the amine N and the nitrido ligand.
DALTON TRANSACTIONS
(2022)
Article
Biochemical Research Methods
Line Marie Nielsen, Niels Bjerre Holm, Lars Olsen, Kristian Linnet
DRUG TESTING AND ANALYSIS
(2016)
Article
Chemistry, Medicinal
Ewa Szymanska, Karla Frydenvang, Darryl S. Pickering, Christian Krintel, Birgitte Nielsen, Ayesheh Kooshki, Linda G. Zachariassen, Lars Olsen, Jette S. Kastrup, Tommy N. Johansen
JOURNAL OF MEDICINAL CHEMISTRY
(2016)
Article
Biophysics
Christian Krintel, Pierre Francotte, Darryl S. Pickering, Lina Juknaite, Jacob Pohlsgaard, Lars Olsen, Karla Frydenvang, Eric Goffin, Bernard Pirotte, Jette S. Kastrup
BIOPHYSICAL JOURNAL
(2016)
Review
Chemistry, Multidisciplinary
Ulf Ryde, Par Soderhjelm
Article
Biochemical Research Methods
Line Marie Nielsen, Niels Bjerre Holm, Sebastian Leth-Petersen, Jesper Langgaard Kristensen, Lars Olsen, Kristian Linnet
DRUG TESTING AND ANALYSIS
(2017)
Article
Chemistry, Inorganic & Nuclear
Geng Dong, Ulf Ryde
INORGANIC CHEMISTRY
(2016)
Article
Biochemistry & Molecular Biology
Geng Dong, Ulf Ryde
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2016)
Article
Biochemistry & Molecular Biology
Octav Caldararu, Martin A. Olsson, Christoph Riplinger, Frank Neese, Ulf Ryde
JOURNAL OF COMPUTER-AIDED MOLECULAR DESIGN
(2017)
Article
Biochemistry & Molecular Biology
Luigi Capoferri, Rasmus Leth, Ernst ter Haar, Arun K. Mohanty, Peter D. J. Grootenhuis, Eduardo Vottero, Jan N. M. Commandeur, Nico P. E. Vermeulen, Flemming Steen Jorgensen, Lars Olsen, Daan P. Geerke
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2016)
Article
Toxicology
Amalie Moller Sorensen, Cecilie Hurup Hansen, Silvia Bonomo, Lars Olsen, Flemming Steen Jorgensen, Johan Juhl Weisser, Andreas Christopher Kretschmann, Bjarne Styrishave
TOXICOLOGY IN VITRO
(2016)
Article
Chemistry, Multidisciplinary
Ola Spjuth, Patrik Rydberg, Egon L. Willighagen, Chris T. Evelo, Nina Jeliazkova
JOURNAL OF CHEMINFORMATICS
(2016)
Article
Chemistry, Physical
Jan H. Jensen, Christopher J. Swain, Lars Olsen
JOURNAL OF PHYSICAL CHEMISTRY A
(2017)
Article
Biochemistry & Molecular Biology
Dario Vazquez-Albacete, Marco Montefiori, Stefan Kol, Mohammed Saddik Motawia, Birger Lindberg Moller, Lars Olsen, Morten H. H. Norholm
Article
Chemistry, Multidisciplinary
Morten Larsen, Cecilie H. Hansen, Tobias B. Rasmussen, Julie Islin, Bjarne Styrishave, Lars Olsen, Flemming Steen Jorgensen
CHEMICAL COMMUNICATIONS
(2017)
Article
Chemistry, Multidisciplinary
Silvia Bonomo, Flemming Steen Jorgensen, Lars Olsen
CHEMISTRY-A EUROPEAN JOURNAL
(2017)