期刊
NEURON
卷 87, 期 3, 页码 521-533出版社
CELL PRESS
DOI: 10.1016/j.neuron.2015.07.001
关键词
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资金
- Deutsche Forschungsgemeinschaft [DR373/3-3]
- DFG Research Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)
- AvH Sofja Kovalevskaja grant
- ERC SytActivity FP7 [260916]
- DFG [SFB 889, DE1951/1-1]
- CNMPB
- CellNetworks cluster of Excellence [EXC81]
- European Commission [LSHM-CT-2005-019055]
- Initial Training Network BrainTrain'' of the Marie-Curie Program
- Collaborative Research Center 1134
- Collaborative Research Center 889
- European Research Council (ERC) [260916] Funding Source: European Research Council (ERC)
Mover, a member of the exquisitely small group of vertebrate-specific presynaptic proteins, has been discovered as an interaction partner of the scaffolding protein Bassoon, yet its function has not been elucidated. We used adeno-associated virus (AAV)-mediated shRNA expression to knock down Mover in the calyx of Held in vivo. Although spontaneous synaptic transmission remained unaffected, we found a strong increase of the evoked EPSC amplitude. The size of the readily releasable pool was unaltered, but short-term depression was accelerated and enhanced, consistent with an increase in release probability after Mover knockdown. This increase in release probability was not caused by alterations in Ca2+ influx but rather by a higher Ca2+ sensitivity of the release machinery, as demonstrated by presynaptic Ca2+ uncaging. We therefore conclude that Mover expression in certain subsets of synapses negatively regulates synaptic release probability, constituting a novel mechanism to tune synaptic transmission.
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