期刊
NEURON
卷 88, 期 3, 页码 492-498出版社
CELL PRESS
DOI: 10.1016/j.neuron.2015.09.047
关键词
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资金
- JSPS/MEXT KAKENHI [15H04261, 15K14321, 26110720, 15K18346]
- JSPS Core-to-Core Program, A. Advanced Research Networks
- Toray Science Foundation
- Uehara Foundation
- Grants-in-Aid for Scientific Research [15K18346, 15H04261, 26110720, 15K14321] Funding Source: KAKEN
Synaptic vesicles are tethered to the active zone where they are docked/primed so that they can fuse rapidly upon Ca2+ influx. To directly study these steps at a CNS presynaptic terminal, we used total internal reflection fluorescence (TIRF) microscopy at the live isolated calyx of Held terminal and measured the movements of single synaptic vesicle just beneath the plasma membrane. Only a subset of vesicles within the TIRF field underwent exocytosis. Following exocytosis, new vesicles (newcomers) approached the membrane and refilled the release sites slowly with a time constant of several seconds. Uniform elevation of the intracellular Ca2+ using flash photolysis elicited an exocytotic burst followed by the sustained component, representing release of the readily releasable vesicles and vesicle replenishment, respectively. Surprisingly, newcomers were not released within a second of high Ca2+. Instead, already-tethered vesicles became release-ready and mediated the replenishment. Our results reveal an important feature of conventional synapses.
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