期刊
JOURNAL OF CENTRAL SOUTH UNIVERSITY OF TECHNOLOGY
卷 18, 期 6, 页码 1849-1856出版社
JOURNAL OF CENTRAL SOUTH UNIV
DOI: 10.1007/s11771-011-0913-1
关键词
magnetic multiwalled carbon nanotubes; paramagnetism; chitosan/silica sol; laccase biosensor; catechol
资金
- Program for Changjiang Scholars and Innovative Research Team in University, China [IRT0719]
- National Natural Science Foundation of China [50978088, 51039001]
- Hunan Key Scientific Research Program, China [2009FJ1010]
- Natural Science Foundation of Hunan Province, China [10JJ7005]
- Hunan Provincial Innovation Foundation For Postgraduate [CX2009B080, CX2010B157]
- Hunan University, China
A simple and rapid strategy to construct laccase biosensor for determination of catechol was investigated. Magnetic multiwalled carbon nanotubes (MMCNT) which possess excellent capability of electron transfer were prepared by chemical coprecipitation method. Scanning electron microscope (SEM) and vibrating sample magnetometer (VSM) were used to identify its surfacetopography and magnetization, respectively. Laccase was immobilized on the MMCNT modified magnetic carbon paste electrode by the aid of chitosan/silica (CS) hybrid membrane. Using current-time detection method, the biosensor shows a linear response related to the concentration of catechol in the range from 10(-7) to 0.165x10(-3) mol/L. The corresponding detection limit is 3.34x10(-8) mol/L based on signal-to-noise ratios (S/N) a parts per thousand yen3 under the optimized conditions. In addition, its response current retains 90% of the original after being stored for 45 d. The results indicate that this proposed strategy can be expected to develop other enzyme-based biosensors.
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