Article
Pharmacology & Pharmacy
Honglei Kang, Yimin Dong, Renpeng Peng, Haiyang Liu, Qian Guo, Kehan Song, Meipeng Zhu, Kaixu Yu, Wei Wu, Feng Li
Summary: This study found that IRE1 plays an important role in the progression of IDD, and inhibition of the IRE1 pathway can suppress the degeneration of NP cells and prevent IDD in vivo. These findings suggest that IRE1 may be a potential target for IDD treatment.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hiroki Ohnishi, Zhongying Zhang, Takashi Yurube, Yoshiki Takeoka, Yutaro Kanda, Ryu Tsujimoto, Kunihiko Miyazaki, Tomoya Matsuo, Masao Ryu, Naotoshi Kumagai, Kohei Kuroshima, Yoshiaki Hiranaka, Ryosuke Kuroda, Kenichiro Kakutani
Summary: This study investigated the effects of AdipoRon on human intervertebral disc (IVD) nucleus pulposus (NP) cells using a three-dimensional in vitro culturing system, and also examined the effects of AdipoRon on rat tail IVD tissues using an in vivo puncture-induced IVD degeneration model. The results demonstrated that AdipoRon suppressed the gene expression of proinflammatory factors and phosphorylation of p65 via the AMPK pathway, thereby alleviating IVD degeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Kaori Suyama, Daisuke Sakai, Masahiko Watanabe
Summary: It has been reported that degenerated and herniated lumbar intervertebral discs express high levels of IL-17, indicating the presence of local immune reactions in patients with low back pain. Although clinical sample analyses from different laboratories confirm this, little is known about how IL-17 is induced in the pathology and its interactions with other inflammatory responses. This conscience review aims to organize current laboratory findings on this topic and provide a trajectory for a comprehensive understanding of the role of IL-17 in the pathology of intervertebral disc disease.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yifan Zhou, Zhiqian Chen, Xiao Yang, Xiankun Cao, Zhihao Liang, Hui Ma, Jie Zhao
Summary: The study revealed that TXNIP promotes pyroptosis in NP cells via the NLRP3/Caspase-1/IL-10 signaling pathway, while Morin shows significant inhibitory effects on the TXNIP/NLRP3/Caspase-1 signaling pathway, potentially serving as an effective therapeutic agent for IDD.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Orthopedics
Rahul Ramanathan, Ayesha Firdous, Qing Dong, Dong Wang, Joon Lee, Nam Vo, Gwendolyn Sowa
Summary: The study reveals that metformin can reduce inflammatory response in rat AF cells under inflammatory and mechanical stress, suggesting its potential role as an anti-degenerative agent.
Article
Orthopedics
Adel Tekari, Alessandro Marazza, Katherine Crump, Paola Bermudez-Lekerika, Benjamin Gantenbein
Summary: This study investigates the role of the extracellular signal-regulated kinase (ERK) pathway in inflammation-induced intervertebral disc degeneration. Blocking the ERK pathway can downregulate catabolic enzymes and proinflammatory cytokines, and upregulate some markers related to intervertebral disc phenotype, suggesting its potential in treating this disorder.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Feng Lv, Longbiao Yang, Jianxiu Wang, Zhixiang Chen, Qizhao Sun, Peiguo Zhang, Chentong Guan, Yanbin Liu
Summary: The study demonstrated that blocking TNFR1 effectively suppressed LPS-induced apoptosis and inflammation in HNPCs, suggesting a potential therapeutic treatment for IDD.
NEUROCHEMICAL RESEARCH
(2021)
Article
Orthopedics
S. Li, X. Pan, Y. Wu, Y. Tu, W. Hong, J. Ren, J. Miao, T. Wang, W. Xia, J. Lu, J. Chen, X. Hu, Y. Lin, X. Zhang, X. Wang
Summary: In this study, the protective effect of Interleukin-37 (IL-37) on intervertebral disc degeneration (IDD) was evaluated. IL-37 was found to decrease during the IDD process and the experimental results showed that IL-37 can suppress the SASP factors level and senescence phenotype in IL-1b treated cells. Animal experiments also demonstrated that IL-37 can alleviate IDD progression in a puncture-induced rat model. Mechanistic studies revealed that IL-37 inhibits IDD progression by downregulating the activation of the NF-kB pathway through the activation of IL-1R8.
OSTEOARTHRITIS AND CARTILAGE
(2023)
Review
Biochemistry & Molecular Biology
Maria de los Angeles Erario, Eduardo Croce, Maria Teresita Moviglia Brandolino, Gustavo Moviglia, Anibal M. Grangeat
Summary: Ozone therapy for extruded disc herniation acts by modulating the immune response, activating macrophages to phagocytose the extruded nucleus pulposus. It facilitates the transition from an inflammatory phase to a reparative phase of macrophages. Further studies are needed to confirm this mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Mohamed H. Yousef, Mohamed Salama, Hassan A. N. El-Fawal, Anwar Abdelnaser
Summary: This study aims to explore the therapeutic utility of GSK3 inhibition in multimodal disease modification and emphasizes the difference in therapeutic value between selective isoform inhibition. The results reveal that GSK3 beta-selective inhibition has superior anti-inflammatory and anti-oxidative efficacy compared to GSK3 alpha-selective and non-selective pan-inhibition.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Cell Biology
Yizhen Huang, Jun Gao, Jianle Wang, Huali Ye, Teng Yao, Yining Xu, Zizheng Chen, Shuying Shen, Jianjun Ma
Summary: This study investigated the role of circPKNOX1 in the progression of IVDD and found that it affects IVDD by regulating the expression of KIAA0355 via miR-370-3p. CircPKNOX1-based therapy may serve as an effective treatment strategy for IVDD.
CELL DEATH DISCOVERY
(2021)
Article
Orthopedics
Jianhang Bao, Wenshuo Gao, Wei Zhang, Dong Wang, Hao Pan
Summary: The purpose of this study was to construct an injectable, sustained-release fibrin gel containing rhein to improve the bioavailability of rhein and evaluate its efficacy in treating intervertebral disc degeneration. The fibrin gel containing rhein exhibited good injectability, sustained release, and biocompatibility. In vitro experiments showed that the rhein@FG could improve the inflammatory microenvironment, regulate ECM metabolic disorders, and inhibit cell pyroptosis. In vivo experiments demonstrated that rhein@FG effectively prevented needle puncture-induced intervertebral disc degeneration in rats.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2023)
Article
Plant Sciences
Dong Wang, Liangping Zhang, Du He, Yujun Zhang, Jianhang Bao, Wenshuo Gao, Wei Cheng, Chengyue Zhu, Hongting Jin, Wei Zhang, Hang Zhu, Hao Pan
Summary: This study investigated the mechanism of action of Qiangjin Zhuang Qufeng mixture (QJZG) on low back pain (LBP) through drug component identification and network pharmacology analysis. The results demonstrated that QJZG delays intervertebral disc degeneration (IVDD) by suppressing inflammation and cell death.
Article
Engineering, Environmental
Dandan Zheng, Wei Chen, Huitong Ruan, Zhengwei Cai, Xiuyuan Chen, Tongtong Chen, Yuhui Zhang, Wenguo Cui, Hao Chen, Hongxing Shen
Summary: A glucose-responsive drug delivery system was developed to deliver metformin for anti-inflammation effect under high glucose conditions, promoting tissue regeneration in diabetes-induced intervertebral disc degeneration model rats. The system adjusted metformin release in response to glucose concentrations, protected mitochondria from ROS damage, inhibited inflammatory pathways, and stimulated extracellular matrix synthesis, ensuring smooth tissue regeneration under diabetic conditions.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Nutrition & Dietetics
Dean M. Cordingley, Stephen M. Cornish, Darren G. Candow
Summary: It is well established that creatine supplementation, especially when combined with resistance training, significantly increases muscle mass and strength. New research also suggests that creatine supplementation has positive effects on bone biology. Creatine influences cellular hydration, energy metabolism, growth factors, muscle protein kinetics, and bone remodeling. It also has anti-inflammatory and anti-catabolic properties, creating a favorable environment for muscle and bone development. Creatine supplementation can decrease inflammation markers and possibly slow down cancerous tumor growth. In terms of musculoskeletal health, creatine supplementation reduces muscle protein breakdown (especially in males) and bone resorption when combined with resistance training.
Article
Orthopedics
Aaron R. Owen, Louis Dagneaux, Afton K. Limberg, Jacob W. Bettencourt, Banu Bayram, Brad Bolon, Daniel J. Berry, Mark E. Morrey, Joaquin Sanchez-Sotelo, Andre J. van Wijnen, Matthew P. Abdel
Summary: This study introduced a novel rat model of arthrofibrosis and characterized its biomechanical, histological, and molecular features, showing significant differences between experimental and control limbs in terms of knee extension and tissue thickness. The proposed model is reproducible, cost-effective, and offers potential for translational investigations into arthrofibrosis pathogenesis and treatment efficacy.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Article
Orthopedics
Caroline Struijk, Wouter Van Genechten, Peter Verdonk, Aaron J. Krych, Allan B. Dietz, Andre J. van Wijnen, Daniel B. F. Saris
Summary: This study demonstrates the feasibility of injecting doses of MSCs (>0.1 million) in meniscus allograft tissue with active cell proliferation, migration, and robust cell survival.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Editorial Material
Orthopedics
Andre J. van Wijnen, Eric A. Lewallen
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME
(2022)
Article
Multidisciplinary Sciences
Coralee E. Tye, Prachi N. Ghule, Jonathan A. R. Gordon, Fleur S. Kabala, Natalie A. Page, Michelle M. Falcone, Kirsten M. Tracy, Andre J. van Wijnen, Janet L. Stein, Jane B. Lian, Gary S. Stein
Summary: This study reveals a long non-coding RNA (MIR181A1HG) that is highly expressed in multipotent mesenchymal stromal cells (MSCs) and down-regulated during osteogenic differentiation. Knockdown of MIR181A1HG impedes cell cycle progression in MSCs and enhances their differentiation into osteo-chondroprogenitors. This study suggests that MIR181A1HG may serve as a regulator of new bone formation in skeletal disorders.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Insug O-Sullivan, Arivarasu Natarajan Anbazhagan, Gurjit Singh, Kaige Ma, Stefan J. Green, Megha Singhal, Jun Wang, Anoop Kumar, Pradeep K. Dudeja, Terry G. Unterman, Gina Votta-Velis, Benjamin Bruce, Andre J. van Wijnen, Hee-Jeong Im
Summary: The study found that probiotic therapy with Lactobacillus acidophilus can rapidly alleviate inflammatory knee joint pain and prevent further progression of osteoarthritis. This treatment also reduces the levels of cartilage-degrading enzymes, pain markers, and inflammatory factors. Additionally, the probiotic treatment significantly alters the fecal microbiota.
Editorial Material
Genetics & Heredity
Andre J. van Wijnen, Erica Golemis, Israel Hanukoglu, Stephen Kwok-Wing Tsui
Article
Multidisciplinary Sciences
Amel Dudakovic, Sofia Jerez, Padmini J. Deosthale, Janet M. Denbeigh, Christopher R. Paradise, Martina Gluscevic, Pengfei Zan, Dana L. Begun, Emily T. Camilleri, Oksana Pichurin, Farzaneh Khani, Roman Thaler, Jane B. Lian, Gary S. Stein, Jennifer J. Westendorf, Lilian I. Plotkin, Andre J. van Wijnen
Summary: High-throughput microRNA sequencing was used to study the differentiation of osteoblasts and identify specific microRNAs involved in osteogenesis. The study found that miR-101a is highly upregulated during osteoblast differentiation and plays a role in accelerating mineralization. Transient elevation of miR-101a reduces the levels of Ezh2, a regulatory enzyme, and promotes bone formation. Male mice with overexpression of miR-101a exhibit enhanced trabecular bone parameters, while no significant changes were observed in females. This suggests that the effect of miR-101a on bone formation is influenced by intricate epigenetic mechanisms, which may differ between sexes.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Insug O-Sullivan, Ranjan Kc, Gurjit Singh, Vaskar Das, Kaige Ma, Xin Li, Fackson Mwale, Gina Votta-Velis, Benjamin Bruce, Arivarasu Natarajan Anbazhagan, Andre J. van Wijnen, Hee-Jeong Im
Summary: The NGF/TrkA signaling pathway plays a crucial role in osteoarthritis pain, involving cellular plasticity in peripheral sensory neurons and angiogenesis in synovial cells. In mice with sensory-neuron-specific deletion of TrkA, the OA-related joint pain was significantly improved.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Orthopedics
Andre J. van Wijnen, Eric A. Lewallen
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME
(2022)
Article
Multidisciplinary Sciences
Roman Thaler, Farzaneh Khani, Ines Sturmlechner, Sharareh S. Dehghani, Janet M. Denbeigh, Xianhu Zhou, Oksana Pichurin, Amel Dudakovic, Sofia S. Jerez, Jian Zhong, Jeong-Heon Lee, Ramesh Natarajan, Ivo Kalajzic, Yong-hui Jiang, David R. Deyle, Eleftherios P. Paschalis, Barbara M. Misof, Tamas Ordog, Andre J. van Wijnen
Summary: The primary function of vitamin C in bone is not just as a cofactor for collagen synthesis, but also as a global regulator of osteogenesis through epigenetic mechanisms. Deficiency in vitamin C disrupts bone integrity and affects osteogenic differentiation. Vitamin C's epigenetic functions are essential for osteoblastogenesis and bone formation.
NATURE COMMUNICATIONS
(2022)
Article
Genetics & Heredity
Parisa Dashti, Jeroen van de Peppel, Roman Thaler, Christopher R. Paradise, Gary S. Stein, Martin A. Montecino, Johannes P. T. M. van Leeuwen, Bram J. van der Eerden, Amel Dudakovic, Andre J. van Wijnen
Summary: This study found that Smyd2 inhibits proliferation and mineral deposition by osteoblasts, and operates parallel to the suppressive effects of Ezh2 and H3K27 trimethylation on osteoblast differentiation.
Editorial Material
Orthopedics
Andre J. van Wijnen, Eric A. Lewallen
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME
(2023)
Review
Biochemistry & Molecular Biology
Praneet Dara, Zeba Farooqui, Fackson Mwale, Chungyoul Choe, Andre J. van Wijnen, Hee-Jeong Im
Summary: Chronic pain conditions caused by arthritis have devastating effects on both individuals and society. The main objective of pharmacological treatments is to reduce pain, and several types of drugs have been considered. Naltrexone, an oral-activated opioid antagonist, has been shown to have beneficial effects on chronic pain conditions such as fibromyalgia and multiple sclerosis.
Article
Orthopedics
Hayman Lui, Cedryck Vaquette, Janet M. Denbeigh, Randy Bindra, Andre J. van Wijnen, Sanjeev Kakar
Summary: This study aims to assess the use of growth factors (BMP2 and GDF5) for bone and ligament regeneration in a multiphasic scaffold in a rabbit knee model. The addition of BMP2 and GDF5 in the BLB scaffold resulted in heterotopic bone formation and ligament failure.
JOURNAL OF WRIST SURGERY
(2023)
Article
Endocrinology & Metabolism
Stephane Blouin, Farzaneh Khani, Phaedra Messmer, Paul Roschger, Markus A. Hartmann, Andre J. van Wijnen, Roman Thaler, Barbara M. Misof
Summary: Vitamin C is crucial for bone health and low levels of serum Vitamin C increase the risk of skeletal fractures. This study examined the effects of Vitamin C on femoral structure, bone formation, and bone mineralization density distribution in mice. The results showed that Vitamin C depletion significantly reduced cortical thickness and bone volume, as well as diminished the amount of newly formed bone tissue. Additionally, Vitamin C supplementation led to higher calcium concentrations and suggested a longer bone tissue age. Female mice were found to be more sensitive to Vitamin C deficiency. These findings highlight the key role of Vitamin C in bone formation rate and its potential in preventing bone-degenerative diseases, especially in females.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)