Article
Biochemistry & Molecular Biology
Shibo Sun, Weiping Xu, Yue Zhang, Yijia Yang, Qiang Ma, Jianqiang Xu
Summary: This study reveals that TrxR1 catalyzes the reduction of menadione in a Sec-independent manner, depending highly on Cys(498) instead. Mutation results showed that the U498C variant supported high-efficiency menadione reduction. The study also reveals that the Sec(498) of TrxR1 is the primary target of menadione.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Jiyoung Lee, Sunmi Lee, Seongchun Min, Sang Won Kang
Summary: This study provides the first evidence that mitochondrial superoxide accumulation is a consequence of necroptosis.
MOLECULES AND CELLS
(2022)
Article
Physiology
Aswathy M. Cheriyan, Adaku C. Ume, Cynthia E. Francis, Keyona N. King, Valerie A. Linck, Yun Bai, Hui Cai, Robert S. Hoover, Heping P. Ma, Jennifer L. Gooch, Clintoria R. Williams
Summary: CnA alpha deficiency stimulates Nox2 upregulation, and NF-kappa B serves as a novel CnA alpha-regulated transcription factor that mediates Nox2 and ROS regulation. These findings suggest potential for selective CNIs to improve long-term outcomes by mitigating renal side effects.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Stephenson B. Owusu, Elodie Hudik, Celine Ferard, Sophie Dupre-Crochet, Eric C. D. K. Addison, Kwasi Preko, Tania Bizouarn, Chantal Houee-Levin, Laura Baciou
Summary: Simulating oxidative stress with gamma irradiation leads to shortened half-lives of neutrophils but pre-activates surviving cells to produce superoxide anions. Incomplete assembly of the NADPH oxidase complex after irradiation significantly enhances neutrophil reactivity.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Medicine, General & Internal
Misaki Matsumoto, Hikari Sawada, Kazumi Iwata, Masakazu Ibi, Nozomi Asaoka, Masato Katsuyama, Kaori Shintani-Ishida, Hiroshi Ikegaya, Shigehiko Takegami, Atsushi Umemura, Chihiro Yabe-Nishimura
Summary: Our study demonstrates that the use of the peptide boronic acid proteasome inhibitor bortezomib significantly enhances the luminescence of the chemiluminescent probe L-012, improving the detection sensitivity of superoxide without affecting background levels. This enhancement is effective in both cell-based and cell-free systems, highlighting a novel feature of bortezomib as a powerful enhancer for the detection of superoxide.
FRONTIERS IN MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Xavier Serfaty, Pauline Lefrancois, Chantal Houee-Levin, Stephane Arbault, Laura Baciou, Tania Bizouarn
Summary: The study reveals that monitoring NADPH oxidation instead of cyt c reduction provides a more accurate measurement of Nox2 activity, indicating a potential underestimation of enzyme activity using the traditional method.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Biochemistry & Molecular Biology
Elizabeth A. Sweeny, Andrew P. Hunt, Allison E. Batka, Simon Schlanger, Nicolai Lehnert, Dennis J. Stuehr
Summary: Nitric oxide (NO) serves as a ubiquitous cell signaling molecule, mediating various processes by activating or inactivating protein targets. Research shows that NO can increase NOX5 activity through heme-dependent effects, shedding light on NOX5 regulation and the impact of NO on hemeprotein activity.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Matea Juric, Varun Rawat, Radhika Amaradhi, Jacek Zielonka, Thota Ganesh
Summary: In this study, a new Nox2 inhibitor, TG15-132, was discovered to prevent oxygen consumption and reactive oxygen species formation. It also showed inhibitory effects on the expression of inflammatory cytokines and iNOS. TG15-132 has a relatively long plasma half-life and excellent brain permeability, making it a potential neuroprotective agent. Furthermore, it does not exhibit toxicity on vital organs or blood biomarkers.
Article
Neurosciences
Yuri Zilberter, Dennis R. Tabuena, Misha Zilberter
Summary: Neurodegenerative diseases (NDDs) causing cognitive impairment and dementia are difficult to treat due to a lack of understanding of their primary initiating factors. Recent research suggests that early oxidative stress caused by NADPH oxidase (NOX) activation may be a shared initiating mechanism among major sporadic NDDs. This study focuses on Alzheimer's disease (AD), Parkinson's disease (PD), and acquired epilepsy, and explores the commonalities in neuroinflammation and NOX involvement to identify therapeutic targets and gain a deeper understanding of the underlying causes of NDDs.
PROGRESS IN NEUROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Chia-Chi Ho, Yu-Cheng Chen, Ming-Hsien Tsai, Hui-Ti Tsai, Chen-Yi Weng, Shaw-Fang Yet, Pinpin Lin
Summary: Epidemiological studies have shown a link between ambient particulate matter exposure and vascular diseases. The research revealed that exposure to ambient PM led to increased cell migration ability in vascular smooth muscle cells and pulmonary arterial smooth muscle cells. Additionally, the study demonstrated that PM induced intracellular reactive oxygen species, activated the NF-kappa B signaling pathway, and increased expression of proinflammatory cytokines in VSMCs. Polyphenolic compounds like curcumin, resveratrol, and gallic acid were found to prevent PM-induced migration by blocking the ROS-dependent NF-kappa B signaling pathway in VSMCs.
Article
Chemistry, Medicinal
Yu Zhang, Xiaohui Tan, Zhen Lin, Fangping Li, Chunyan Yang, Haiying Zheng, Lingyu Li, Huazhong Liu, Jianghua Shang
Summary: The study demonstrated that fucoidan (FPS) effectively inhibits the expression induction of urate transporters URAT1 and GLUT9 induced by uric acid, through repressing the activation of NF-kappa B, JNK, and PI3K/Akt signal pathways.
Review
Biochemistry & Molecular Biology
Sameera Kumar, Daret St Clair
Summary: Prostate cancer is common in men, and oxidative stress caused by free radicals and reactive oxygen species could be a contributing factor. The superoxide dismutase family plays a crucial role in removing oxygen-derived reactive oxygen species, and increased superoxide dismutase activity may have a protective effect against prostate cancer. Understanding the role of superoxide dismutase in radioresistance has led to the development of interventions targeting the NF-kappa B pathway for prostate cancer treatment.
Article
Immunology
Rong Hu, Libo Chen, Xiaolong Chen, Zhong Xie, Chao Xia, Yong Chen
Summary: Aloperine has been shown to inhibit osteoclast activity and formation mediated by RANKL, indicating its potential as a new drug for the prevention and treatment of postmenopausal osteoporosis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Andrea Guidarelli, Andrea Spina, Gloria Buffi, Giulia Blandino, Mara Fiorani, Orazio Cantoni
Summary: Exposure of U937 cells to arsenite stimulates calcium release from IP3R, which leads to a cascade of events including effects on mitochondria and oxidoreductases. Higher concentrations of arsenite promote more calcium release and activate additional mechanisms, but without affecting calcium homeostasis. Dependent calcium release from IP3R plays a pivotal role in the effects of arsenite on calcium homeostasis.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Review
Ecology
Colleen M. Hansel, Julia M. Diaz
Summary: Reactive oxygen species (ROS) production plays essential roles in signaling, growth, biological interactions, and physiochemical defense systems in various organisms. Additionally, biological ROS production significantly influences natural fluxes of ROS in the ocean, affecting the fate of carbon, metals, oxygen, and climate-relevant gases.
ANNUAL REVIEW OF MARINE SCIENCE, VOL 13, 2021
(2021)
Article
Oncology
Ying-Nai Wang, Heng-Huan Lee, Chao-Kai Chou, Wen-Hao Yang, Yongkun Wei, Chun-Te Chen, Jun Yao, Jennifer L. Hsu, Cihui Zhu, Haoqiang Ying, Yuanqing Ye, Wei-Jan Wang, Seung-Oe Lim, Weiya Xia, How-Wen Ko, Xiuping Liu, Chang-Gong Liu, Xifeng Wu, Huamin Wang, Donghui Li, Laura R. Prakash, Matthew H. Katz, Yaan Kang, Michael Kim, Jason B. Fleming, David Fogelman, Milind Javle, Anirban Maitra, Mien-Chie Hung
Article
Oncology
Pei-Chih Lee, Yueh-Fu Fang, Hirohito Yamaguchi, Wei-Jan Wang, Tse-Ching Chen, Xuan Hong, Baozhen Ke, Weiya Xia, Yongkun Wei, Zhengyu Zha, Yan Wang, Han-Pin Kuo, Chih-Wei Wang, Chih-Yen Tu, Chia-Hung Chen, Wei-Chien Huang, Shu-Fen Chiang, Lei Nie, Junwei Hou, Chun-Te Chen, Longfei Huo, Wen-Hao Yang, Rong Deng, Katsuya Nakai, Yi-Hsin Hsu, Shih-Shin Chang, Tai-Jan Chiu, Jun Tang, Ran Zhang, Li Wang, Bingliang Fang, Ting Chen, Kwok-Kin Wong, Jennifer L. Hsu, Mien-Chie Hung
Article
Cell Biology
Junwei Hou, Rongce Zhao, Weiya Xia, Chiung-Wen Chang, Yun You, Jung-Mao Hsu, Lei Nie, Yeh Chen, Yu-Chuan Wang, Chunxiao Liu, Wei-Jan Wang, Yun Wu, Baozhen Ke, Jennifer L. Hsu, Kebin Huang, Zu Ye, Yi Yang, Xianghou Xia, Yintao Li, Chia-Wei Li, Bin Shao, John A. Tainer, Mien-Chie Hung
NATURE CELL BIOLOGY
(2020)
Article
Virology
Sheng-Teng Huang, Yeh Chen, Wei-Chao Chang, Hsiao-Fan Chen, Hsiang-Chun Lai, Yu-Chun Lin, Wei-Jan Wang, Yu-Chuan Wang, Chia-Shin Yang, Shao-Chun Wang, Mien-Chie Hung
Summary: The study found that extracts from Scutellaria barbata D. Don can inhibit the enzyme activities of M-pro and TMPRSS2, effectively inhibiting SARS-CoV-2 infection through a TMPRSS2-dependent mechanism.
Article
Biochemistry & Molecular Biology
You-Zhe Lin, Yi-Chun Shen, Wan-Rong Wu, Wei-Jan Wang, Yuan-Liang Wang, Chen-Yuan Lin, Mien-Chie Hung, Shao-Chun Wang
Summary: The study shows that imatinib can inhibit the expression of ACE2 gene, reduce tissue expression of ACE2 in lungs and renal tubules, and effectively block the cell entry of SARS-CoV-2. This suggests that receptor tyrosine kinases could play a role in COVID-19 infection and be potential therapeutic targets in combination with conventional care.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Wei-Jan Wang, Hong-Yue Lai, Fei Zhang, Wan-Jou Shen, Pei-Yu Chu, Hsin-Yin Liang, Ying-Bin Liu, Ju-Ming Wang
Summary: This study highlights that obesity is associated with increased risks and decreased survival in patients with GBC. Leptin plays a role in GBC growth and resistance to gemcitabine, possibly through the pSTAT3/CEBPD/MCL1 axis. Targeting this pathway may provide a new therapeutic strategy for improving gemcitabine efficacy in GBC patients.
Article
Integrative & Complementary Medicine
Guan-Yu Chen, Yi-Cheng Pan, Tung-Ying Wu, Tsung-You Yao, Wei-Jan Wang, Wan-Jou Shen, Azaj Ahmed, Shu-Ting Chan, Chih-Hsin Tang, Wei-Chien Huang, Mien-Chie Hung, Juan-Cheng Yang, Yang-Chang Wu
Summary: This study identified 39 natural products from the Taiwan Database of Extracts and Compounds that can potentially prevent the binding of viral spike proteins with human ACE2 proteins, thus blocking the entry of SARS-CoV-2 into cells. In silico and in vitro experiments demonstrated the binding affinities of these natural products and their inhibitory effects on viral infection.
JOURNAL OF TRADITIONAL AND COMPLEMENTARY MEDICINE
(2022)
Correction
Nutrition & Dietetics
Jilu Lang, Yanan Shao, Jiehao Liao, Jia Chen, Xuewen Zhou, Rong Deng, Wei-Jian Wang, Xian Sun
BRITISH JOURNAL OF NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Yeh Chen, Wen-Hao Yang, Hsiao-Fan Chen, Li-Min Huang, Jing-Yan Gao, Cheng-Wen Lin, Yu-Chuan Wang, Chia-Shin Yang, Yi-Liang Liu, Mei-Hui Hou, Chia-Ling Tsai, Yi-Zhen Chou, Bao-Yue Huang, Chian-Fang Hung, Yu-Lin Hung, Wei-Jan Wang, Wen-Chi Su, Vathan Kumar, Yu-Chieh Wu, Shih-Wei Chao, Chih-Shiang Chang, Jin-Shing Chen, Yu-Ping Chiang, Der-Yang Cho, Long-Bin Jeng, Chang-Hai Tsai, Mien-Chie Hung
Summary: This study screened a library of FDA-approved compounds and identified the antimalarial drug tafenoquine (TFQ) as a potential inhibitor of the SARS-CoV-2 main protease (M-pro). The crystal structure of the M-pro-TFQ complex revealed the binding and reshaping of the substrate-binding pocket, blocking the catalytic activity. TFQ was also found to inhibit a human trans-membrane protease. A derivative of TFQ, compound 7, showed improved efficacy in inhibiting protease activity and may therefore be effective against SARS-CoV-2 and its variants.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Oncology
Hoang Dang Khoa Ta, Wei-Jan Wang, Nam Nhut Phan, Nu Thuy An Ton, Gangga Anuraga, Su-Chi Ku, Yung-Fu Wu, Chih-Yang Wang, Kuen-Haur Lee
Summary: LSM4 expression is elevated in breast cancer and correlates with poor survival outcomes. Infiltration levels of immune cells are positively associated with LSM4 expression, suggesting its potential role in breast cancer prognosis. MetaCore and GlueGo analyses indicate LSM family members, particularly LSM4, are significantly correlated with oncogenesis in breast cancer patients.
Article
Biochemistry & Molecular Biology
Cheng-Wei Chou, Yu-Hsiu Hsieh, Su-Chi Ku, Wan-Jou Shen, Gangga Anuraga, Hoang Dang Khoa Ta, Kuen-Haur Lee, Yu-Cheng Lee, Cheng-Hsien Lin, Chih-Yang Wang, Wei-Jan Wang
Summary: The study revealed that OSBPL3, OSBPL8, OSBPL10, and OSBPL11 are highly upregulated in PDAC, with lower expressions associated with longer overall survival. The functions of OSBPL family members are mainly connected with various cancer signaling pathways and immune infiltrates.
Article
Health Care Sciences & Services
Gangga Anuraga, Wei-Jan Wang, Nam Nhut Phan, Nu Thuy An Ton, Hoang Dang Khoa Ta, Fidelia Berenice Prayugo, Do Thi Minh Xuan, Su-Chi Ku, Yung-Fu Wu, Vivin Andriani, Muhammad Athoillah, Kuen-Haur Lee, Chih-Yang Wang
Summary: NEK family genes are overexpressed in breast cancer, with NEK2/6/8 associated with poor prognosis in distant metastasis-free survival. NEK2 is linked to cell cycle, DNA repair, and immune infiltration signaling pathways.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Oncology
Shao-Chun Wang, Yeh Chen, Yu-Chuan Wang, Wei-Jan Wang, Chia-Shin Yang, Chia-Ling Tsai, Mei-Hui Hou, Hsiao-Fan Chen, Yi-Chun Shen, Mien-Chie Hung
AMERICAN JOURNAL OF CANCER RESEARCH
(2020)
Article
Oncology
Xian Sun, Chia-Wei Li, Wei-Jan Wang, Mei-Kuang Chen, Hui Li, Yun-Ju Lai, Jennifer L. Hsu, Paul B. Koller, Li-Chuan Chan, Pei-Chih Lee, Fang-Ju Cheng, Clinton Yam, Gong-Yan Chen, Mien-Chie Hung
AMERICAN JOURNAL OF CANCER RESEARCH
(2020)
Article
Oncology
Wei-Jan Wang, Jung-Mao Hsu, Ying-Nai Wang, Heng-Huan Lee, Hirohito Yamaguchi, Hsin-Wei Liao, Mien-Chie Hung
AMERICAN JOURNAL OF CANCER RESEARCH
(2019)