Article
Biochemical Research Methods
Nisitha Wijewantha, Morgan M. Eikanger, Ryan M. Antony, Rashaun A. Potts, Khosrow Rezvani, Grigoriy Sereda
Summary: The study aimed to develop a therapeutic strategy using nanoparticles to selectively deliver the anticancer molecule VTD to colorectal cancer cells and release it on demand, enhancing efficiency through enzyme-triggered release with minimal side effects, making it a promising anticancer drug.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Cell Biology
Olivia R. Grafinger, Genya Gorshtein, Tyler Stirling, Jennifer Geddes-McAlister, Marc G. Coppolino
Summary: Integrin signaling is crucial for the establishment of focal adhesions and invadopodia formation in malignant cancer cells, promoting tumor cell invasion and metastasis through localized adhesion and degradation of the ECM. Inhibition of 81 integrin hinders the internalization and recycling of MT1-MMP, ultimately leading to a loss of invasive behavior.
CELLULAR SIGNALLING
(2021)
Review
Biochemistry & Molecular Biology
Katerina Strouhalova, Ondrej Tolde, Daniel Rosel, Jan Brabek
Summary: MT1-MMP (MMP-14) is a versatile protease involved in regulating extracellular matrix degradation, activation of other proteases, as well as various cellular processes such as migration and viability in both physiological and pathological conditions. The cytoplasmic tail of MT1-MMP, consisting of the final 20 C-terminal amino acids, plays a crucial role in determining its localization, signal transduction capabilities, and interaction with other proteins. This review summarizes the involvement of the cytoplasmic tail in regulating and executing the functions of MT1-MMP, providing insights into cellular adhesion and invasion mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Luis Placido, Yair Romero, Mariel Maldonado, Fernanda Toscano-Marquez, Remedios Ramirez, Jazmin Calyeca, Ana L. Mora, Moises Selman, Annie Pardo
Summary: This study explored the role of MMP14 in idiopathic pulmonary fibrosis (IPF) and found that its deficiency exacerbates fibrotic injury and affects repair, possibly through its anti-senescent activity. Increased MMP14 in IPF may represent an anti-fibrotic mechanism overwhelmed by the strong profibrotic microenvironment of the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Deanna Maybee, Nicole L. Ink, Mohammad A. M. Ali
Summary: This review article discusses the subcellular localization and intracellular roles of matrix metalloproteinases (MMPs), focusing on membrane-type-1 matrix metalloproteinase (MT1-MMP) and matrix metalloproteinase-2 (MMP-2). The article highlights the diverse subcellular localizations of these enzymes and their involvement in cell migration, gene expression, and tumor migration and invasion. The authors suggest that targeting nuclear MT1-MMP or MMP-2 may lead to the development of new therapies for cancer and other diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Megan Brasher, Shawn C. Chafe, Paul C. McDonald, Oksana Nemirovsky, Genya Gorshtein, Zachary J. Gerbec, Wells S. Brown, Olivia R. Grafinger, Matthew Marchment, Esther Matus, Shoukat Dedhar, Marc G. Coppolino
Summary: This study demonstrates that disrupting the interaction between Stx4 and Munc18c can dramatically alter the progression of tumor metastasis, suggesting it as a potential therapeutic target.
MOLECULAR CANCER RESEARCH
(2022)
Review
Oncology
Hannah L. M. Spencer, Steven D. Shnyder, Paul M. Loadman, Robert A. Falconer
Summary: This review summarizes the expression and potential role of Membrane - type 1 matrix metalloproteinase (MT1-MMP) in osteosarcoma (OS), and assesses its therapeutic potential as a drug target. The findings suggest that exploiting the expression and proteolytic capacity of MT1-MMP could lead to the discovery of novel, safer and more selective drugs for the treatment of OS.
JOURNAL OF CANCER METASTASIS AND TREATMENT
(2022)
Article
Multidisciplinary Sciences
Xuanming Guo, Jianli Cao, Jian-Piao Cai, Jiayan Wu, Jiangang Huang, Pallavi Asthana, Sheung Kin Ken Wong, Zi-Wei Ye, Susma Gurung, Yijing Zhang, Sheng Wang, Zening Wang, Xin Ge, Hiu Yee Kwan, Aiping Lyu, Kui Ming Chan, Nathalie Wong, Jiandong Huang, Zhongjun Zhou, Zhao-Xiang Bian, Shuofeng Yuan, Hoi Leong Xavier Wong
Summary: The study identifies MT1-MMP as a critical host protease for solACE2-mediated SARS-CoV-2 infection. SARS-CoV-2 infection leads to increased activation of MT1-MMP that is colocalized with ACE2 in human lung epithelium. MT1-MMP directly cleaves ACE2 to release solACE2, thus facilitating cell entry of SARS-CoV-2.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Anna M. M. Knapinska, Gary Drotleff, Cedric Chai, Destiny Twohill, Alexa Ernce, Dorota Tokmina-Roszyk, Isabella Grande, Michelle Rodriguez, Brad Larson, Gregg B. B. Fields
Summary: In this study, we used a fluorescent substrate, membrane type 1 matrix metalloproteinase (MT1-MMP), and collagen embedded tumor spheroids to quantify MT1-MMP activity. The results showed that MT1-MMP activity was closely correlated with glioma spheroid invasion. The application of MT1-MMP inhibitors reduced proteolytic activity and glioma spheroid invasion. The presence of MT1-MMP in glioma spheroids was confirmed. Thus, invasion was dependent on MT1-MMP activity, and inhibitors of MT1-MMP and invasion could be conveniently screened.
Article
Biochemistry & Molecular Biology
Juan Juan Yang, Ye Hua Wang, Jiao Yin, Hong Leng, Song Dong Shen
Summary: The polysaccharide isolated from Ulva prolifera was found to inhibit the invasive potential of non-small cell lung cancer cells by suppressing the Myc-mediated MMP-9 gene transcription through the MAPK signaling pathway. These results provide a theoretical basis for the application of polysaccharides in preventing lung cancer metastasis.
Article
Oncology
Hung-yi Chen, Mei-due Yang, Yu-cheng Chou, Yi-shih Ma, Shu-fen Peng, Ching-lung Liao, Po-yuan Chen, Te-chun Hsia, Jin-cherng Lien, Chung-hung Chen
Summary: Ouabain suppresses cell metastasis through multiple signaling pathways in AGS cells, reducing cell proliferation, viability, and motility, while also inhibiting cell migration and invasion.
ANTICANCER RESEARCH
(2021)
Article
Cell Biology
Tomoko Matsuzaki, Douglas R. Keene, Emi Nishimoto, Makoto Noda
Summary: The study suggests that RECK, ADAMTS10, and MT1-MMP cooperate to support the formation of robust FBN1 fibers by regulating proteolytic activity and morphology, despite their initially perceived opposing functions.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Immunology
Laurent Devel, Nicolas Guedeney, Sarah Bregant, Animesh Chowdhury, Mickael Jean, Patrick Legembre
Summary: CD95L, a member of the tumor necrosis family superfamily, plays a crucial role in both apoptotic and non-apoptotic signaling. However, the different forms and functions of this ligand, as well as the metalloproteases involved, need further investigation. Understanding these aspects can contribute to the development of targeted therapies for CD95/CD95L.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Plant Sciences
Guozhu Tang, Shuchi Wu, Bingyu Zhao, Barry S. Flinn
Summary: The plant extracellular matrix (ECM) undergoes significant modifications during plant growth, development and stress responses. This study focuses on the characterization of At5-MMP, a constitutively expressed MMP in Arabidopsis thaliana. The study found that At5-MMP exhibits maximum proteolytic activity at pH 7.5 and requires a signal peptide for targeting to the cell periphery. Additionally, WAK1 was identified as a potential substrate for At5-MMP cleavage.
JOURNAL OF PLANT BIOLOGY
(2022)
Review
Cell Biology
Izabela Figiel, Patrycja K. Kruk, Monika Zareba-Koziol, Paulina Rybak, Monika Bijata, Jakub Wlodarczyk, Joanna Dzwonek
Summary: The extracellular matrix plays a critical role in synaptic function, with MMP-9 and Rho GTPases identified as key factors in regulating synaptic plasticity. MMP-9 contributes to dynamic remodeling by cleaving ECM components, while Rho GTPases control cellular processes in brain physiology and pathology.
Article
Hematology
Lobna Alkebsi, Xintao Wang, Hiroshi Ohkawara, Masahiko Fukatsu, Hirotaka Mori, Takayuki Ikezoe
Summary: Studies have shown that dasatinib may induce EndMT in vascular-endothelial cells, contributing to vascular toxicity, while bosutinib could effectively prevent this transition from occurring.
INTERNATIONAL JOURNAL OF HEMATOLOGY
(2021)
Review
Hematology
Hirotaka Mori, Hiroshi Ohkawara, Ryuichi Togawa, Mami Rikimaru, Yoko Shibata, Takayuki Ikezoe
Summary: Current studies on the diagnosis and treatment of DIC complicated by COVID-19 mainly focus on ISTH and SIC scores as well as the effectiveness of LMWH, indicating a need for further research to compare with other available DIC scoring systems and anticoagulants.
INTERNATIONAL JOURNAL OF HEMATOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Masashi Kamioka, Naoko Hijioka, Minoru Nodera, Shinya Yamada, Takashi Kaneshiro, Yasuchika Takeishi
Summary: The electrophysiological parameter LATD <= 7 ms is associated with intramural origins of left ventricular outflow tract-ventricular tachyarrhythmias, with some anatomical limitations.
JOURNAL OF INTERVENTIONAL CARDIAC ELECTROPHYSIOLOGY
(2022)
Article
Immunology
Rongqing Li, Na Sun, Xin Chen, Xueqin Li, Jie Zhao, Wanpeng Cheng, Hui Hua, Masahiko Fukatsu, Hirotaka Mori, Hiroshi Takahashi, Hiroshi Ohkawara, Miwa Fukami, Masatoshi Okamoto, Yoichi Hamazaki, Kuiyang Zheng, Jing Yang, Takayuki Ikezoe
Summary: The JAK2(V617F) mutation leads to increased production of IL-6, possibly by enhancing STAT3 activation. Additionally, the mutation also protects PKM1 protein through inhibition of CMA-mediated degradation, ultimately promoting IL-6 production.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Hematology
Hirotaka Mori, Masahiko Fukatsu, Hiroshi Ohkawara, Yuka Oka, Yuki Kataoka, Shunsuke Taito, Takayuki Ikezoe
Summary: The diagnosis of plasmablastic lymphoma, plasmablastic myeloma, and plasmablastic neoplasm may be arbitrary in some cases due to the inconsistent diagnostic criteria in previous studies. A scoping review was conducted to analyze the heterogeneity in diagnostic criteria, revealing variations in criteria for PBL but consistency for PBM. An algorithm developed in the study included PBN for the first time, with significant differences in results among three diagnostic algorithms. Hematologists and pathologists are recommended to pay more attention to the differential diagnosis of PBL, PBM, and PBN.
INTERNATIONAL JOURNAL OF HEMATOLOGY
(2021)
Article
Cell Biology
Yoshinori Koga, Ami Sotokawauchi, Yuichiro Higashimoto, Yuri Nishino, Naoki Hashizume, Tatsuyuki Kakuma, Jun Akiba, Yoshiaki Tanaka, Takanori Matsui, Minoru Yagi, Sho-ichi Yamagishi
Summary: The study suggests that RAGE-aptamer could attenuate multiple organ damage in LPS-injected septic mice by inhibiting inflammatory reactions and improving survival rates.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2021)
Article
Oncology
Shiori Tsuruhisa, Takanori Matsui, Yoshinori Koga, Ami Sotokawauchi, Minoru Yagi, Sho-Ichi Yamagishi
Summary: PEDF may exert antitumor effects in AGE-exposed breast cancer cells by suppressing NADPH oxidase-induced ROS generation and VEGF and MMP-9 expression via interaction with LR. This study suggests that pharmacological upregulation or restoration of PEDF may inhibit the growth and metastasis of breast cancer, as PEDF expression is decreased in breast cancer tissues.
Article
Geriatrics & Gerontology
Nobuhiro Tahara, Atsuko Tahara, Shoko Maeda-Ogata, Hanae Yoshimura, Munehisa Bekki, Yoichi Sugiyama, Akihiro Honda, Sachiyo Igata, Yuri Nishino, Takanori Matsui, Ako Fukami, Mika Enomoto, Hisashi Adachi, Yoshihiro Fukumoto, Sho-ichi Yamagishi
Summary: The study found a correlation between increased serum levels of glyceraldehyde-derived AGEs and aging-related disorders, but it is unclear which structurally distinct AGEs could be reliable biomarkers. The findings suggest that measuring urine pentosidine-to-creatinine ratio with a newly developed ELISA kit may help identify high-risk patients for fall-related fractures.
REJUVENATION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Masahiko Fukatsu, Hiroshi Ohkawara, Xintao Wang, Lobna Alkebsi, Miki Furukawa, Hirotaka Mori, Miwa Fukami, Shin-ichi Fukami, Takahiro Sano, Hiroshi Takahashi, Kayo Harada-Shirado, Satoshi Kimura, Koichi Sugimoto, Kazuei Ogawa, Takayuki Ikezoe
Summary: This study found that plasma concentrations of Gas6 and soluble Mer were higher in patients with severe sepsis or septic ALI/ARDS. The selective Mer inhibitor UNC2250 was shown to effectively inhibit LPS-induced lung injury in mouse models, suggesting its potential therapeutic use in treating inflammatory responses in ALI/ARDS.
Article
Cardiac & Cardiovascular Systems
Tomonori Watanabe, Hiroaki Watanabe, Hitoshi Hachiya, Masafumi Sato, Tadayuki Mitama, Takafumi Okuyama, Ayako Yokota, Masashi Kamioka, Takahiro Komori, Tomoyuki Kabutoya, Yasushi Imai, Kazuomi Kario
Summary: Additional therapeutic strategies for atrial fibrillation (AF) beyond pulmonary vein isolation (PVI) have not been established. Remodeling of the left atrium (LA) could impact AF recurrence post-PVI. We investigated the impact of unipolar voltage (UV) criteria for the LA posterior wall (LA-PW) on AF recurrence post-PVI.
JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Michishige Terasaki, Keita Shibata, Yusaku Mori, Tomomi Saito, Takanori Matsui, Makoto Ohara, Tomoyasu Fukui, Keiji Hasumi, Yuichiro Higashimoto, Koji Nobe, Sho-ichi Yamagishi
Summary: SMTP-44D inhibits the formation of atherosclerotic plaques by blocking the AGEs-RAGE pathway and reduces macrophage infiltration and AGEs accumulation in the aortic roots. It also reduces foam cell formation in macrophages by suppressing the Cdk5-CD36 pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Masashi Kamioka, Hisaki Makimoto, Tomonori Watanabe, Hiroaki Watanabe, Takafumi Okuyama, Takashi Kaneshiro, Naoko Hijioka, Ayako Yokota, Takahiro Komori, Tomoyuki Kabutoya, Yasushi Imai, Kazuomi Kario
Summary: The relationship between local unipolar voltage (UV) in the pulmonary vein (PV)-ostia and left atrial wall thickness (LAWT) and their utility as indices of outcome after atrial fibrillation (AF) ablation were investigated. It was found that both UV and LAWT were significantly higher in patients with atrial tachyarrhythmia (ATA) recurrence, and they had a strong positive correlation. Combining UV and LAWT allows for stratification of recurrence risk and suggests a tailored ablation strategy based on LA tissue properties.
Article
Hematology
Shin-ichi Fuchida, Koji Kawamura, Kazutaka Sunami, Nobuhiro Tsukada, Shiro Fujii, Hiroshi Ohkawara, Kensuke Usuki, Atsushi Wake, Shinya Endo, Ken Ishiyama, Yasunori Ueda, Yukinori Nakamura, Toshihiro Miyamoto, Takahiro Fukuda, Tatsuo Ichinohe, Yoshiko Atsuta, Hiroyuki Takamatsu
Summary: Autologous stem cell transplantation (ASCT) is the standard treatment for eligible patients with light-chain (AL) amyloidosis. However, little is known about ASCT in Asian populations. Our study investigated the outcomes and prognostic factors of ASCT in AL amyloidosis patients. The results showed that overall survival improved over time, but poor performance status and cardiac involvement had negative impacts on prognosis. Early mortality after ASCT was relatively high, and brain natriuretic peptide (BNP) level was a useful prognostic factor.
TRANSPLANTATION AND CELLULAR THERAPY
(2022)
Article
Integrative & Complementary Medicine
Mitsuyoshi Miyata, Shunsuke Iwata, Chie K. Mifude, Masahiro Tajima, Mayu Kameyama, Masaki Ihara, Takanori Matsui, Sho-Ichi Yamagishi, Hiroyuki Ishitobi, Shigeru Miyaki, Kuniyoshi Kaseda
Summary: This study aimed to find effective microalgae from hot springs for the treatment of inflammatory diseases. A novel strain of green algae, Mucidosphaerium sp., isolated from a Beppu hot spring, showed anti-inflammatory and antioxidant properties, making it a potentially useful alternative medicine for skin and joint inflammatory disorders.
ALTERNATIVE THERAPIES IN HEALTH AND MEDICINE
(2021)
Article
Endocrinology & Metabolism
Ami Sotokawauchi, Takanori Matsui, Yuichiro Higashimoto, Yuri Nishino, Yoshinori Koga, Minoru Yagi, Sho-ichi Yamagishi
Summary: In obese type 2 diabetic mice, RAGE-aptamer was found to inhibit tubular damage, reduce oxidative stress in kidney tissues, and improve insulin resistance.
DIABETES & VASCULAR DISEASE RESEARCH
(2021)
