期刊
JOURNAL OF CELLULAR PHYSIOLOGY
卷 227, 期 3, 页码 1157-1167出版社
WILEY
DOI: 10.1002/jcp.22836
关键词
clusterin; apoptosis; Bcl-XL
资金
- National Research Foundation of Korea [R13-2005-012-01001-1]
Clusterin (CLU), a glycoprotein, is involved in apoptosis, producing two alternatively spliced isoforms in various cell types. The pro-apoptotic CLU appears to be a nuclear isoform (nuclear clusterin; nCLU), and the secretory CLU (sCLU) is thought to be anti-apoptotic. The detailed molecular mechanism of nCLU as a pro-apoptotic molecule has not yet been clear. In the current study, overexpressed nCLU induced apoptosis in human kidney cells. Biochemical studies revealed that nCLU sequestered Bcl-XL via a putative BH3 motif in the C-terminal coiled coil (CC2) domain, releasing Bax, and promoted apoptosis accompanied by activation of caspase-3 and cytochrome c release. These results suggest a novel mechanism of apoptosis mediated by nCLU as a pro-apoptotic molecule. J. Cell. Physiol. 227: 11571167, 2012. (C) 2011 Wiley Periodicals, Inc.
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