Article
Biochemistry & Molecular Biology
Orie Adachi, Hideki Sugii, Tomohiro Itoyama, Shoko Fujino, Hiroshi Kaneko, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Junko Obata, Shinichiro Yoshida, Masataka Kadowaki, Risa Sugiura, Mhd Safwan Albougha, Hidefumi Maeda
Summary: The study aims to clarify the biological functions of decorin in the healing and regeneration of wounded periodontal tissue. The results demonstrate that decorin promotes osteoblastic differentiation of periodontal ligament stem cells and contributes to bone healing through ERK1/2 signaling pathway.
Article
Environmental Sciences
Yifen Shen, Chao Liu, Tao Yang, Ying Tang, Yihang Shen, Yongchun Gu
Summary: In this study, the effects of e-cigarette exposure on human gingival mesenchymal stem cells (GMSCs) and periodontal ligament stem cells (PDLSCs) were examined through RNA-seq analysis. The results showed that e-cigarette exposure primarily affected immunoregulation and inflammatory responses in GMSCs, and the microenvironment, differentiation, and corticosteroid response in PDLSCs. Additionally, a novel mechanism involving KDM6B-mediated H3K27me3 on enhancers for gene transcription regulation was identified.
ENVIRONMENTAL POLLUTION
(2023)
Article
Dentistry, Oral Surgery & Medicine
Sinan Mert, Hanna Malyaran, Rogerio B. Craveiro, Michael Wolf, Ali Modabber, Willi Jahnen-Dechent, Sabine Neuss
Summary: Clinical experience suggests that wounds in the maxilla heal faster and more efficiently compared with the mandible. This study aimed to investigate whether differences in the proliferation and differentiation of periodontal ligament stem cells (PDLSC) from the maxilla and mandible contribute to this discrepancy. The results showed that maxillary PDLSC exhibited higher proliferation and differentiation potential compared to mandibular PDLSC, indicating a possible cell-based explanation for the different healing efficacy in the maxilla and mandible.
JOURNAL OF PERIODONTOLOGY
(2023)
Review
Pharmacology & Pharmacy
Edward J. Calabrese
Summary: This paper provides a detailed assessment of hormetic dose responses by human periodontal ligament stem cells, induced by a broad range of chemicals. The underlying mechanistic foundations of these dose responses are clarified, showing them to be widespread and highly conserved across different types of stem cells. The therapeutic implications of these hormetic dose responses are explored, highlighting their potential in various applications.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Jianjia Li, Zhifa Wang, Xiangyu Huang, Zhaodan Wang, Zehao Chen, Runting Wang, Zhao Chen, Wei Liu, Buling Wu, Fuchun Fang, Wei Qiu
Summary: This study conducted a dynamic proteomic analysis of osteogenic differentiation of hPDLSCs, revealing a regulatory mechanism and potential perspectives for periodontal regeneration research. The results indicated that oxidative phosphorylation and redox-sensitive signaling pathways play important roles in osteogenesis, with SOD2 identified as a key protein in the process.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Medicine, Research & Experimental
Lingping Tan, Zeyuan Cao, Huan Chen, Yunyi Xie, Le Yu, Chuanqiang Fu, Wei Zhao, Yan Wang
Summary: The study showed that curcumin could enhance cell proliferation and increase bone tissue regeneration ability of transplanted hPDLSCs under oxidative stress conditions. Curcumin reduced apoptosis and promoted osteogenesis of hPDLSCs through various mechanisms, suggesting its potential clinical application in tissue regeneration.
Article
Dentistry, Oral Surgery & Medicine
Yu Ye, Lin Fu, Liu Liu, Tong Xiao, Ana Gloria Cuba Manduca, Jinhua Yu
Summary: This study investigated the impact of hypoxia on human periodontal ligament stem cells (PDLSCs) using RNA sequencing. The expression profiles of circRNAs, lncRNAs, and miRNAs were found to be significantly altered in PDLSCs cultured in low oxygen conditions. Specific circRNAs and lncRNAs may have a competitive role in the differentiation of PDLSCs.
Article
Immunology
Christian Behm, Alice Blufstein, Johannes Gahn, Andreas Moritz, Xiaohui Rausch-Fan, Oleh Andrukhov
Summary: Human periodontal ligament-derived mesenchymal stromal cells (hPDL-MSCs) have bi-directional interaction with CD4(+) T lymphocytes, and their immunomodulatory abilities are enhanced by 25-hydroxyvitamin D-3 (25(OH)D-3) through boosting the expression of immunomediators. The influence of 25(OH)D-3 on this interaction is still unknown.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Yingzhi Gu, Yuxing Bai
Summary: This study aimed to investigate the effect of crocin on the proliferation and osteogenic differentiation of human periodontal ligament stem cells (PDLSCs) in vitro and in vivo. Crocin treatment significantly promoted PDLSC mRNA levels, protein expression, mineralized nodule formation, and ALP activity. Crocin also enhanced the phosphorylation of beta-catenin and cyclin D1. Inhibition of the Wnt/beta-catenin pathway partially reversed crocin-mediated promotion of PDLSC osteogenic differentiation.
Article
Biochemistry & Molecular Biology
Risa Sugiura, Sayuri Hamano, Atsushi Tomokiyo, Daigaku Hasegawa, Shinichiro Yoshida, Hideki Sugii, Shoko Fujino, Orie Adachi, Masataka Kadowaki, Daiki Yamashita, Hidefumi Maeda
Summary: Periodontal ligament stem cells (PDLSCs) play important roles in maintaining, repairing, and regenerating periodontal ligament (PDL) tissue. This study identified the transcription factor PAX9 as a regulator of ECM production in human PDL cells, which plays a crucial role in the differentiation of iNCs into iPDLSCs.
Article
Dentistry, Oral Surgery & Medicine
Dongqing Ai, Yuanyuan Yin, Xuyun Xia, Sihan Yang, Yu Sun, Jie Zhou, Han Qin, Xiaohui Xu, Jinlin Song
Summary: This study aimed to explore an appropriate model of Type 2 diabetes (T2DM) in human periodontal ligament stem cells (hPDLSCs) and uncover the mechanisms linking T2DM and periodontitis. The researchers found that palmitic acid is a promising candidate for developing T2DM model in hPDLSCs, as it induced inflammation, insulin resistance, and pyroptosis, while inhibiting osteogenic differentiation.
Article
Dentistry, Oral Surgery & Medicine
Alice Blufstein, Christian Behm, Barbara Kubin, Johannes Gahn, Andreas Moritz, Xiaohui Rausch-Fan, Oleh Andrukhov
Summary: The study suggests that the transcriptional activity of VDR is reduced under inflammatory conditions, potentially diminishing the effectiveness of vitamin D(3) supplementation during periodontal treatment.
JOURNAL OF PERIODONTOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Qing Shao, ShiWei Liu, Chen Zou, Yilong Ai
Summary: This study found that miR-590-3p promotes osteogenic differentiation of hPDLSCs in periodontitis by targeting LSD1 transcription and upregulating H3K4me2 methylation to enhance OSX transcription.
Article
Chemistry, Multidisciplinary
Jiayao Sun, Zhiguo Wang, Peng Liu, Yingzhe Hu, Tingting Li, Jianbo Yang, Pengyu Gao, Quanchen Xu
Summary: This study aimed to explore the effects of exosomes derived from human gingival mesenchymal stem cells (GMSC-Exo) on the inflammatory response of periodontal ligament stem cells (PDLSCs) in an inflammatory microenvironment. The study found that GMSC-Exo attenuated the inflammatory response of PDLSCs by regulating the expression of NF-kappa B signaling and Wnt5a.
FRONTIERS IN CHEMISTRY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Liu Wang, Xiangfen Li, Yao Song, Lan Zhang, Ling Ye, Xuedong Zhou, Dongzhe Song, Dingming Huang
Summary: This study explored the effect of BMP9 on the osteogenic differentiation of inflammatory periodontal ligament stem cells (PDLSCs). The results showed that BMP9 promoted osteogenic differentiation of PDLSCs, but also upregulated the expression of inflammatory markers. NELL1 played an important role in this process by activating the MAPK/p38/ERK signaling pathway, reducing the expression of inflammatory genes induced by BMP9, and enhancing the osteogenic effect of the cells.
JOURNAL OF PERIODONTOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
R. Bright, G. M. Thiele, J. Manavis, T. R. Mikuls, J. B. Payne, P. M. Bartold
JOURNAL OF PERIODONTAL RESEARCH
(2018)
Article
Cell & Tissue Engineering
Esther Camp, Clara Pribadi, Peter J. Anderson, Andrew C. W. Zannettino, Stan Gronthos
STEM CELLS AND DEVELOPMENT
(2018)
Article
Nanoscience & Nanotechnology
Soraya Rasi Ghaemi, Bahman Delalat, Stan Gronthos, Morgan R. Alexander, David A. Winkler, Andrew L. Hook, Nicolas H. Voelcker
ACS APPLIED MATERIALS & INTERFACES
(2018)
Article
Hematology
Agnieszka Arthur, Thao M. Nguyen, Sharon Paton, Andrew C. W. Zannettino, Stan Gronthos
EXPERIMENTAL HEMATOLOGY
(2019)
Editorial Material
Dentistry, Oral Surgery & Medicine
Peter Mark Bartold, Thomas E. Van Dyke
JOURNAL OF CLINICAL PERIODONTOLOGY
(2019)
Article
Dentistry, Oral Surgery & Medicine
Mark Bartold, Stan Gronthos, David Haynes, Saso Ivanovski
JOURNAL OF CLINICAL PERIODONTOLOGY
(2019)
Article
Genetics & Heredity
Dimitrios Cakouros, Sarah Hemming, Kahlia Gronthos, Renjing Liu, Andrew Zannettino, Songtao Shi, Stan Gronthos
EPIGENETICS & CHROMATIN
(2019)
Article
Cell & Tissue Engineering
Dongqing Yang, Stan Gronthos, Sandra Isenmann, Howard A. Morris, Gerald J. Atkins
STEM CELLS INTERNATIONAL
(2019)
Review
Clinical Neurology
Maria R. Gancheva, Karlea L. Kremer, Stan Gronthos, Simon A. Koblar
FRONTIERS IN NEUROLOGY
(2019)
Article
Biochemistry & Molecular Biology
Pingping Han, Peter Mark Bartold, Carlos Salomon, Saso Ivanovski
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Pingping Han, Peter Mark Bartold, Carlos Salomon, Saso Ivanovski
Summary: The study compared salivary sEVs from periodontitis patients, healthy controls, and gingivitis patients, finding that global 5mC hypermethylation in salivary sEVs can serve as a potential biomarker with high sensitivity and specificity for distinguishing periodontitis patients. Periodontitis sEVs showed significantly increased levels of LPS+ OMVs, global 5mC methylation, and periodontal pathogens, which were more pronounced in sEVs than whole saliva.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Multidisciplinary
Shu Hua, Peter Mark Bartold, Karan Gulati, Corey Stephen Moran, Saso Ivanovski, Pingping Han
Summary: This review discusses the biogenesis of extracellular vesicles (EVs), particularly small EVs (sEVs) derived from periodontal and dental pulp cells, and their therapeutic potential. The study found that these EVs have various functions in vitro and in vivo, indicating a significant potential for regenerative applications.
Article
Dentistry, Oral Surgery & Medicine
Andrew Liaw, Chun Liu, Mark Bartold, Saso Ivanovski, Pingping Han
Summary: This study aimed to investigate the expression levels of HDACs in human saliva in different periodontal conditions. The results suggest that HDAC4, 8, and 9 could have potential as biomarkers for periodontal diagnosis.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Nicholas Smith, Suzanna Shirazi, Dimitrios Cakouros, Stan Gronthos
Summary: Many important epigenetic changes occur during early skeletal development and throughout life, influenced by factors such as aging, disease, and lifestyle. Epigenetics explores the heritable changes in gene expression resulting from environmental changes without any alterations in DNA sequence. Cellular epigenetic profiles are dynamic and regulated through mechanisms including histone modifications, non-coding RNA-associated gene silencing, and DNA methylation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Geriatrics & Gerontology
Dimitrios Cakouros, Stan Gronthos