Article
Biochemistry & Molecular Biology
Francesca Camera, Eleonora Colantoni, Tomas Garcia-Sanchez, Barbara Benassi, Claudia Consales, Adeline Muscat, Leslie Vallet, Luis M. Mir, Franck Andre, Caterina Merla
Summary: In recent years, the application of nanosecond pulsed electric fields has been investigated for novel medical purposes. This study focused on examining molecular calcium fluxes induced by different fractionated protocols of short electric pulses. The researchers found that fractionated doses of nanosecond pulsed electric fields did not increase cell electrosensitization, but targeted activation of calcium-dependent genes was observed based on the duration of calcium flux.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Pi-Cheng Cheng, Ruo-Ciao Cheng, Rong-Chi Huang
Summary: Glutamate induces Ca2+ signaling in the suprachiasmatic nucleus (SCN) by activating intracellular mechanisms involving Na+ loads, Na+/K+-ATPase (NKA), and Na+/Ca2+-exchanger (NCX). The presence of Na+ loads leads to slower Ca2+ clearance and increased rebound Ca2+ suppression. However, even in the absence of external Na+, additional Ca2+ handlers are involved in the slower Ca2+ clearance induced by glutamate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Hayden E. Fowler, Philipp Rothemund, Christoph Keplinger, Timothy J. White
Summary: The integration of soft, stimuli-responsive materials in robotic systems allows for delicate object manipulation. Electrical control of mechanical response in liquid crystal elastomers (LCEs) results in rapid deformation with little hysteresis. The directional expansion and shape transformation capabilities of LCE actuators demonstrate promising applications in robotics.
ADVANCED MATERIALS
(2021)
Review
Plant Sciences
Hyo-Jun Lee, Pil Joon Seo
Summary: Plants have evolved stress-response mechanisms involving cytosolic Ca2+ signaling, with ion channels and transporters playing important roles in perceiving stress stimuli and facilitating Ca2+ influx. These early signaling events not only allow plants to adapt to environmental stresses, but also inspire the identification of additional stress sensors.
TRENDS IN PLANT SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Vincenzo Nassisi, Aurora Mazzei, Gianmarco Del Vecchio, Antonio Calisi, Luciano Velardi, Pietro Alifano, Tiziano Verri
Summary: In this experiment, groups of zebrafish embryos were exposed to radiations of different frequencies and intensities using new prototype devices. The radiations included static, extremely low-frequency, low-frequency, very-high-frequency, and ultra-high-frequency fields. The exposure led to alterations in body pigmentation of the zebrafish embryos and larvae. These findings contribute to understanding the effects of magnetic radiation on living organisms.
APPLIED SCIENCES-BASEL
(2023)
Review
Biochemistry & Molecular Biology
Qiuyan Dong, Lukas Wallrad, Bader O. Almutairi, Joerg Kudla
Summary: Adverse variations of abiotic environmental cues can cause stress to plants, leading to reduced crop yield and negative impact on ecosystem stability. Ca2+ plays multifaceted roles in abiotic stress responses, serving as a versatile second messenger and connecting stress perception to signal transduction.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Environmental Sciences
Marta Silva Muniz, Katharina Halbach, Igor Caue Alves Araruna, Rafael Xavier Martins, Bettina Seiwert, Oliver Lechtenfeld, Thorsten Reemtsma, Davi Farias
Summary: Moxidectin exhibited acute toxicity to zebrafish embryos by decreasing hatching rates, altering enzymatic activity, and strongly accumulating in embryos, mainly distributed in the head and eyes.
ENVIRONMENTAL POLLUTION
(2021)
Article
Engineering, Environmental
Yan Wang, Tong Xiao, Shiyu Zuo, Jinquan Wan, Zhicheng Yan, Bin Zhu, XiaoLong Zhang
Summary: By tuning the defects of the C-N co-ordination of the support, the polarized electric field (PEF) characteristics are changed, which affects the electron transport behavior. This study proposes a novel PEF regulation strategy and explores its mechanism for safe treatment of ECs.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Review
Chemistry, Physical
Lei Chen, Jin-Tao Ren, Zhong-Yong Yuan
Summary: In recent years, there has been a growing interest in utilizing advanced photo-/electrocatalysts for efficient energy conversion and environmental remediation. The construction of internal electric fields has emerged as a promising technique for enhancing various catalytic processes. This review provides a comprehensive overview of the latest advances in constructing internal electric fields and their impact on photo(electro)catalytic and electrocatalytic activity, as well as their potential applications in energy and environmental fields.
ADVANCED ENERGY MATERIALS
(2023)
Article
Developmental Biology
Abijeet Singh Mehta, Pin Ha, Kan Zhu, ShiYu Li, Kang Ting, Chia Soo, Xinli Zhang, Min Zhao
Summary: During the migration of cranial neural crest cells (CNCCs), biophysical cues such as electric fields play a crucial role, with mammalian CNCCs demonstrating directional migration towards the anode in direct current electric fields. This migration behavior is voltage-dependent and influenced by the strength of the electric field.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemical Research Methods
Arezoo Khalili, Ellen van Wijngaarden, Georg R. Zoidl, Pouya Rezai
Summary: This study investigates the effects of dopamine signaling pathway on the electric response of zebrafish larvae. It is found that dopamine antagonists decrease locomotor activities, while dopamine agonists do not induce similar behaviors. Moreover, exposing larvae pretreated with dopamine antagonists to specific dopamine agonists restores the electric locomotion. These findings reveal a correlation between electric response and dopamine signaling pathway, and demonstrate the conditioning effect of dopamine receptor modulation on electric-induced zebrafish larvae locomotion.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Chemistry, Physical
Nadia G. Leonard, Rakia Dhaoui, Teera Chantarojsiri, Jenny Y. Yang
Summary: Electric fields play a crucial role in modifying the free energy landscape and impacting reactivity, yet they are rarely considered in synthetic design. This Perspective discusses the commonalities of electric field effects across different platforms and identifies practical challenges in applying them to mediate reactivity in synthetic molecular systems.
Article
Biochemistry & Molecular Biology
Hassan Osman Alhassan Elsaid, Hakon Tjeldnes, Mariell Rivedal, Camille Serre, Oystein Eikrem, Einar Svarstad, Camilla Tondel, Hans-Peter Marti, Jessica Furriol, Janka Babickova
Summary: Fabry disease is an X-linked metabolic disorder caused by a deficiency of lysosomal alpha-galactosidase A. The disease is characterized by renal insufficiency and cardiovascular involvement. Recently, there has been a need for additional markers to monitor the disease's progression and treatment response. A gla-knockout zebrafish model was used to identify alternative biomarkers in Gb3-free conditions, expanding the understanding of the pathophysiology and potential therapeutic targets for Fabry disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Environmental
Zsuzsanna Kolbert, Attila Ordog
Summary: The study highlights the important regulatory role of nitric oxide (NO) in plant responses to metalloids, mitigating oxidative stress and promoting antioxidant defense. Silicon intensifies the stress-mitigating effect of NO on plants, while selenium, arsenic, and antimony may cause toxicity through the accumulation of NO or reactive nitrogen species.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Ling Huang, Audrey Holtzinger, Ishaan Jagan, Michael BeGora, Ines Lohse, Nicholas Ngai, Cristina Nostro, Rennian Wang, Lakshmi B. Muthuswamy, Howard C. Crawford, Cheryl Arrowsmith, Steve E. Kalloger, Daniel J. Renouf, Ashton A. Connor, Sean Cleary, David F. Schaeffer, Michael Roehrl, Ming-Sound Tsao, Steven Gallinger, Gordon Keller, Senthil K. Muthuswamy
Article
Biochemistry & Molecular Biology
Xiangpeng Dai, Wenjian Gan, Xiaoning Li, Shangqian Wang, Wei Zhang, Ling Huang, Shengwu Liu, Qing Zhong, Jianping Guo, Jinfang Zhang, Ting Chen, Kouhei Shimizu, Francisco Beca, Mirjam Blattner, Divya Vasudevan, Dennis L. Buckley, Jun Qi, Lorenz Buser, Pengda Liu, Hiroyuki Inuzuka, Andrew H. Beck, Liewei Wang, Peter J. Wild, Levi A. Garraway, Mark A. Rubin, Christopher E. Barbieri, Kwok-Kin Wong, Senthil K. Muthuswamy, Jiaoti Huang, Yu Chen, James E. Bradner, Wenyi Wei
Article
Oncology
Maud-Emmanuelle Gilles, Liangliang Hao, Ling Huang, Rajesha Rupaimoole, Pedro P. Lopez-Casas, Emilia Pulver, Jong Cheol Jeong, Senthil K. Muthuswamy, Manuel Hidalgo, Sangeeta N. Bhatia, Frank J. Slack
CLINICAL CANCER RESEARCH
(2018)
Article
Cell Biology
David M. Graham, Ling Huang, Kenneth R. Robinson, Mark A. Messerli
JOURNAL OF CELL SCIENCE
(2013)
Article
Biochemistry & Molecular Biology
Fleur M. Ferguson, Behnam Nabet, Srivatsan Raghavan, Yan Liu, Alan L. Leggett, Miljan Kuljanin, Radha L. Kalekar, Annan Yang, Shuning He, Jinhua Wang, Raymond W. S. Ng, Rita Sulahian, Lianbo Li, Emily J. Poulin, Ling Huang, Jost Koren, Nora Dieguez-Martinez, Sergio Espinosa, Zhiyang Zeng, Cesear R. Corona, James D. Vastall, Ryoma Ohi, Taebo Sim, Nam Doo Kim, Wayne Harshbarger, Jose M. Lizcano, Matthew B. Robers, Senthil Muthaswamy, Charles Y. Lin, A. Thomas Look, Kevin M. Haigis, Joseph D. Mancias, Brian M. Wolpin, Andrew J. Aguirre, William C. Hahn, Kenneth D. Westover, Nathanael S. Gray
NATURE CHEMICAL BIOLOGY
(2020)
Article
Chemistry, Medicinal
Fleur M. Ferguson, Yan Liu, Wayne Harshbarger, Ling Huang, Jinhua Wang, Xianming Deng, Stephen J. Capuzzi, Eugene N. Muratov, Alexander Tropsha, Senthil Muthuswamy, Kenneth D. Westover, Nathanael S. Gray
JOURNAL OF MEDICINAL CHEMISTRY
(2020)
Article
Oncology
Spas Dimitrov-Markov, Javier Perales-Paton, Bruno Bockorny, Ana Dopazo, Manuel Munoz, Natalia Banos, Victoria Bonilla, Camino Menendez, Yolanda Duran, Ling Huang, Sofia Perea, Senthil K. Muthuswamy, Fatima Al-Shahrour, Pedro P. Lopez-Casas, Manuel Hidalgo
MOLECULAR CANCER THERAPEUTICS
(2020)
Article
Medicine, Research & Experimental
Ling Huang, Bruno Bockorny, Indranil Paul, Dipikaa Akshinthala, Pierre-Oliver Frappart, Omar Gandarilla, Arindam Bose, Veronica Sanchez-Gonzalez, Emily E. Rouse, Sylvain D. Lehoux, Nicole Pandell, Christine M. Lim, John G. Clohessy, Joseph Grossman, Raul Gonzalez, Sofia Perea Del Pino, George Daaboul, Mandeep S. Sawhney, Steven D. Freedman, Alexander Kleger, Richard D. Cummings, Andrew Emili, Lakshmi B. Muthuswamy, Manuel Hidalgo, Senthil K. Muthuswamy
Article
Cell & Tissue Engineering
Ling Huang, Ridhdhi Desai, Daniel N. Conrad, Nayara C. Leite, Dipikaa Akshinthala, Christine Maria Lim, Raul Gonzalez, Lakshmi B. Muthuswamy, Zev Gartner, Senthil K. Muthuswamy
Summary: Induction of human pluripotent stem cells towards pancreatic ductal and acinar organoids has provided a renewable source for modeling exocrine development and diseases. The study demonstrated the lineage tropism and plasticity for oncogene action in the human pancreas, with specific oncogenes like GNAS(R201C) and KRAS(G12D) showing differential effects on ductal and acinar organoids both in culture and in vivo.
Article
Multidisciplinary Sciences
Shawn C. Chafe, Frederick S. Vizeacoumar, Geetha Venkateswaran, Oksana Nemirovsky, Shannon Awrey, Wells S. Brown, Paul C. McDonald, Fabrizio Carta, Andrew Metcalfe, Joanna M. Karasinska, Ling Huang, Senthil K. Muthuswamy, David F. Schaeffer, Daniel J. Renouf, Claudiu T. Supuran, Franco J. Vizeacoumar, Shoukat Dedhar
Summary: The study identified a redox homeostasis network involving the iron-sulfur cluster enzyme NFS1 in the survival mechanisms governed by the tumor hypoxia-induced pH regulator CAIX. Targeting CAIX while depleting NFS1 or blocking cyst(e)ine availability enhanced ferroptosis and inhibited tumor growth significantly. Altering intracellular pH and iron homeostasis through inhibiting CAIX activity may lead to the development of innovative therapeutic strategies for solid tumors to overcome hypoxia- and acidosis-mediated tumor progression and therapeutic resistance.
Article
Oncology
Joseph E. Grossman, Lakshmi Muthuswamy, Ling Huang, Dipikaa Akshinthala, Sofia Perea, Raul S. Gonzalez, Leo L. Tsai, Jonah Cohen, Bruno Bockorny, Andrea J. Bullock, Benjamin Schlechter, Mary Linton B. Peters, Catherine Conahan, Supraja Narasimhan, Christine Lim, Roger B. Davis, Robert Besaw, Mandeep S. Sawhney, Douglas Pleskow, Tyler M. Berzin, Martin Smith, Tara S. Kent, Mark Callery, Senthil K. Muthuswamy, Manuel Hidalgo
Summary: This pilot feasibility trial investigated the use of patient-derived organoids (PDOs) to guide personalized therapy for pancreatic ductal adenocarcinoma (PDAC). The study found that the drug sensitivity of PDOs can predict the clinical outcome of patients and is correlated with treatment responses.
CLINICAL CANCER RESEARCH
(2022)
Article
Oncology
Qingda Meng, Shanshan Xie, G. Kenneth Gray, Mohammad H. Dezfulian, Weilin Li, Ling Huang, Dipikaa Akshinthala, Elizabeth Ferrer, Catherine Conahan, Sofia Perea Del Pino, Joseph Grossman, Stephen J. Elledge, Manuel Hidalgo, Senthil K. Muthuswamy
Summary: This study reports a platform for expanding tumor-targeting T cells from the peripheral blood of patients with pancreatic cancer, and identifies the NKG2A-HLA-E axis as an important checkpoint for CD8+ T cells in pancreatic cancer. Empirical identification of tumor-targeting TCRs suitable for TCR therapy was demonstrated.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Oncology
Shira Orr, Ling Huang, James Moser, Dina Stroopinsky, Omar Gandarilla, Cori DeCicco, Jessica Liegel, Cansu Tacettin, Adam Ephraim, Giulia Cheloni, Daniela Torres, Donald Kufe, Jacalyn Rosenblatt, Manuel Hidalgo, Senthil K. Muthuswamy, David Avigan
Summary: The study demonstrates that the use of personalized cancer vaccines can expand pancreatic cancer specific lymphocytes, leading to significant therapeutic effects in mouse models, including reducing tumor volume and prolonging survival.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2023)
Article
Endocrinology & Metabolism
Sevim Kahraman, Ercument Dirice, Giorgio Basile, Danielle Diegisser, Jahedul Alam, Bente B. Johansson, Manoj K. Gupta, Jiang Hu, Ling Huang, Chew-Li Soh, Danwei Huangfu, Senthil K. Muthuswamy, Helge Raeder, Anders Molven, Rohit N. Kulkarni
Summary: The interaction between exocrine and endocrine cells through the transfer of mutant CEL protein from pancreatic acinar cells to beta-cells causes dysfunction and loss of beta-cells, leading to the development of MODY8.
Article
Biochemistry & Molecular Biology
Brian M. Kobylkevich, Anyesha Sarkar, Brady R. Carlberg, Ling Huang, Suman Ranjit, David M. Graham, Mark A. Messerli