Article
Biology
Qing Zhang, Feng Lin, Jianyong Huang, Chunyang Xiong
Summary: Cell phenotype heterogeneity within tumor tissue, especially due to epithelial-mesenchymal transition (EMT), plays a role in cancer invasion and metastasis. In this study, heterotypic tumor spheroids containing both epithelial and EMT cancer cells were generated and it was found that EMT cells dominated the periphery of the spheroid. Furthermore, EMT cells acted as leader cells to enhance the migration efficiency of epithelial cancer cells and promote dispersion and invasion of the spheroids through force transition and heterophilic N-cadherin/E-cadherin adhesion complexes. Impairment of this adhesion complex formation prevented EMT cells from guiding epithelial cancer cell migration and blocked the dispersion of tumor spheroids.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Review
Oncology
Arpita Datta, Shuo Deng, Vennila Gopal, Kenneth Chun-Hong Yap, Clarissa Esmeralda Halim, Mun Leng Lye, Mei Shan Ong, Tuan Zea Tan, Gautam Sethi, Shing Chuan Hooi, Alan Prem Kumar, Celestial T. Yap
Summary: The cellular process of EMT is a critical mechanism in cancer metastasis, involving reorganization of the cytoskeleton composed of actin filaments, microtubules, and intermediate filaments. Understanding the physiological functions of the cytoskeleton and targeting key components for EMT regulation may offer promising approaches for cancer therapy, particularly in tackling multidrug-resistant cancer cells.
Article
Oncology
Weichao Dan, Yizeng Fan, Tao Hou, Yi Wei, Bo Liu, Taotao Que, Bixin Yu, Jin Zeng, Lei Li
Summary: This study found that silibinin inhibits the migration, invasion and epithelial-mesenchymal transition of prostate cancer cells by regulating the autophagic degradation of Yes-associated protein, suggesting it as a potential novel treatment strategy for metastatic castration-resistant prostate cancer.
Review
Biochemistry & Molecular Biology
Rahul Advani, Sara Luzzi, Emma Scott, Caroline Dalgliesh, Joachim Weischenfeldt, Jennifer Munkley, David J. Elliott
Summary: This article discusses the expression of splicing regulators ESRP1 and ESRP2 in aggressively proliferating primary prostate tumors as markers of disease progression. It suggests that ESRP1 and ESRP2 act as lineage survival oncogenes in prostate cancer and highlights the need to identify the gene expression targets controlled by these regulators to develop targeted therapies.
Article
Reproductive Biology
Qiao Ping Xu, Kui Deng, Zhen Zhang, Hongkai Shang
Summary: Ovarian cancer is a growing global disease, and there is an urgent need for chemotherapy and targeted therapy. SLFN5 plays an important role in malignancy, but its specific function in ovarian cancer cells is still unclear. This study suggests that SLFN5 expression is related to the progression and malignancy of ovarian cancer, and its silencing has a significant inhibitory effect on the invasion and metastasis ability of ovarian cancer cells. Therefore, SLFN5 may serve as a potential target for future drug development in the treatment of ovarian cancer.
JOURNAL OF OVARIAN RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Yulin Wang, Zhiyue Tu, Wei Zhao, Liping Wang, Jing Jiang, Lili Gu, Meng Li, Lujian Jiang, Yibin Wang, Yinglu Bi
Summary: This study found that PLCB1 expression is elevated in gastric cancer and is associated with poor prognosis. It promotes cell migration and invasion by regulating actin cytoskeleton rearrangement and epithelial-mesenchymal transition process. Targeting PLCB1 may be a potential strategy to improve the prognosis of gastric cancer patients.
BIOCHEMICAL GENETICS
(2023)
Article
Nanoscience & Nanotechnology
Qianqian Guo, Yang Dong, Yanhua Zhang, Hao Fu, Chuanrong Chen, Liting Wang, Xupeng Yang, Ming Shen, Jian Yu, Meiwan Chen, Jiali Zhang, Yourong Duan
Summary: The study found that PTX-resistant prostate cancer cells exhibited EMT and enhanced metastasis. By developing a novel nanoparticle carrier, the researchers successfully reversed PTX resistance and improved efficacy, with good tumor-targeting ability and minimal side effects.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Integrative & Complementary Medicine
Yih-Shou Hsieh, Shu-Chen Chu, Shih-Chien Huang, Shao-Hsuan Kao, Meng-Shuan Lin, Pei-Ni Chen
Summary: The research showed that gossypol inhibited the invasion and migration of human cervical cancer cells by targeting the FAK signaling pathway and reversing TGF-beta 1-induced EMT.
AMERICAN JOURNAL OF CHINESE MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Aitao Sun, JinBo Li, Weijing Kong, Xiaodong Jiang
Summary: This study investigates the expression and underlying mechanisms of the immunoglobulin superfamily containing leucine-rich repeat (ISLR) in gastric cancer (GC). The findings suggest that ISLR is overexpressed in GC tumor tissues and cells and is associated with lymph node metastasis, tumor size, and clinical stage. Additionally, ISLR regulates the epithelial-mesenchymal transition (EMT) signaling pathway and affects cell viability, proliferation, migration, and invasion in GC cells. These results provide new evidence for the role of ISLR in GC progression.
Article
Endocrinology & Metabolism
Mao-Gui Pang, Xin Wang, Shuang-Shuang Le, Song-Tao Ji, Tong-Tong Guo, Yun Zhou, Bo-Da Wang, Kun Ji, Yi Liu, Fa-Dong Wang, Xiao-Di Zhao, Xin Wang
Summary: This study revealed that downregulation of TPM1 promotes colorectal cancer progression by enhancing cell migration, invasion, and proliferation, as well as destabilizing the cytoskeleton. On the other hand, overexpression of TPM1 significantly inhibits the EMT process. These findings suggest that TPM1 has an important role in colorectal cancer metastasis.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2023)
Review
Oncology
Samantha Gogola, Michael Rejzer, Hisham F. Bahmad, Wassim Abou-Kheir, Yumna Omarzai, Robert Poppiti
Summary: Prostate cancer is the second most common cancer in men worldwide, and treatment options include radiation therapy, surgery, and active surveillance. However, in most cases, the cancer eventually becomes castration-resistant. The transition from androgen-dependent to androgen-independent state is not well understood, but epithelial-to-non-epithelial transition plays a crucial role. This review summarizes the factors and pathways involved in this transition, as well as the diagnostic and prognostic biomarkers.
Article
Biochemistry & Molecular Biology
Qiaosi Tang, Ashley Lento, Kensuke Suzuki, Gizem Efe, Tatiana Karakasheva, Apple Long, Veronique Giroux, Mirazul Islam, E. Paul Wileyto, Andres J. Klein-Szanto, Hiroshi Nakagawa, Adam Bass, Anil K. Rustgi
Summary: The study reveals that TP53 mutation is the most common in ESCC, while Rab11-FIP1 gene is significantly downregulated in mutant p53 tumor cells. Genetic knockdown of Rab11-FIP1 results in increased organoid size and promotes tumor cell invasion through mutant p53.
Article
Medicine, Research & Experimental
Mei-Tsz Su, Pei-Yin Tsai, Chia-Yih Wang, Hui-Ling Tsai, Pao-Lin Kuo
Summary: Preeclampsia is a severe gestational hypertensive disorder that poses a major threat to maternal and fetal health. Aspirin has been shown to effectively reduce the incidence of premature preeclampsia among high-risk pregnant women, possibly by suppressing miR-200 family-mediated cell functions. This study demonstrates a potential mechanism explaining aspirin's effect on preeclampsia prevention and provides a therapeutic target for disease intervention.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Samuel A. Vilchez Mercedes, Federico Bocci, Mona Ahmed, Ian Eder, Ninghao Zhu, Herbert Levine, Jose N. Onuchic, Mohit Kumar Jolly, Pak Kin Wong
Summary: In this study, the researchers used a combination of immunocytochemistry, single cell biosensors, and computational modeling to show that the transcription factor Nrf2 functions as a phenotypic stability factor for hybrid epithelial/mesenchymal cells during collective cancer migration. They also demonstrated that Nrf2 and EMT signaling are spatially coordinated near the leading edge, and that the Nrf2-EMT-Notch network enhances the expression of certain genes associated with the formation of leader cells and protruding tips.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Claudia M. Garcia-Cuellar, Miguel Santibanez-Andrade, Yolanda I. Chirino, Raul Quintana-Belmares, Rocio Morales-Barcenas, Ericka Marel Quezada-Maldonado, Yesennia Sanchez-Perez
Summary: The study demonstrated that PM10 exposure induces EMT in lung cancer cells, leading to an increase in invasive phenotype. TGFB1 plays a crucial role in this process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Gaoyuan Cao, Hong Lam, Joseph A. Jude, Nikhil Karmacharya, Mengyuan Kan, William Jester, Cynthia Koziol-White, Blanca E. Himes, Geoffrey L. Chupp, Steven S. An, Reynold A. Panettieri
Summary: In most cells, cAMP acts as a short-lived second messenger, confined to the intracellular space and controlled by G protein actions. However, in human airway smooth muscle cells, activation of the cell-surface receptor β(2)AR leads to the release of cAMP into the extracellular space. This release is mediated by the ABC transporter ABCC1 and may have implications for the treatment of airflow obstruction in asthma.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Cell Biology
Magdalena Prechova, Zuzana Adamova, Anna-Lena Schweizer, Miloslava Maninova, Andreas Bauer, Delf Kah, Samuel M. Meier-Menches, Gerhard Wiche, Ben Fabry, Martin Gregor
Summary: This study reveals the crucial role of plectin in supporting intercellular junctions through organizing cortical cytoskeletal networks in epithelial sheets. Deficiency of plectin leads to abnormal cytoarchitecture, elevated intracellular tension, and reduced intercellular cohesion, resulting in destabilization of tissues under mechanical stress.
JOURNAL OF CELL BIOLOGY
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Francesco De Pascali, Michael Ippolito, Emily Wolfe, Konstantine E. Komolov, Nathan Hopfinger, Douglas Lemenze, Roger S. Armen, Steven S. An, Charles P. Scott, Jeffrey L. Benovic
Review
Genetics & Heredity
Donghwa Kim, Alina Tokmakova, Jung-A A. Woo, Steven S. An, William A. Goddard, Stephen B. Liggett
Summary: GPCRs are a superfamily of receptors activated by a variety of ligands, able to activate different signaling pathways with varying therapeutic effects. Biased ligands may offer new opportunities for drug development by selectively targeting specific pathways, though challenges in understanding structure-function relationships need to be addressed.
MOLECULAR DIAGNOSIS & THERAPY
(2022)
Article
Physiology
Brian T. Deeney, Gaoyuan Cao, Sarah Orfanos, Jordan Lee, Mengyuan Kan, Blanca E. Himes, Vishal Parikh, Cynthia J. Koziol-White, Steven S. An, Reynold A. Panettieri Jr
Summary: Epinephrine plays a role in physiological functions by activating adrenergic receptors in various organs, but its functional role on adrenergic receptors in human bronchial smooth muscle is still not clear. New research has found that human bronchial smooth muscle cells express a high abundance of α1 adrenergic receptor subtype B, and activation of these receptors can induce bronchial smooth muscle cell constriction, potentially leading to asthma attacks. This discovery provides a new basis for reevaluating the role of α1 adrenergic receptor inhibition in asthma treatment.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Ajay P. Nayak, Steven S. An
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Francesco De Pascali, Michael Ippolito, Emily Wolfe, Konstantin E. Komolov, Nathan Hopfinger, Douglas Lemenze, Nicholas Kim, Roger S. Armen, Steven S. An, Charles P. Scott, Jeffrey L. Benovic
Summary: This study identified several G(s)-biased agonists that relieve airflow obstruction by activating G(s) signaling pathway through β(2)-adrenoceptor. These biased compounds showed minimal β-arrestin-mediated effects and induced minimal desensitization of the receptor in primary HASM cells. These findings may have implications for the treatment of asthma and the development of safer drugs.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Critical Care Medicine
Kwangmi Ahn, Raymond B. Penn, Satish Rattan, Reynold A. Panettieri, Benjamin F. Voight, Steven S. An
Summary: Using Mendelian randomization analysis, this study demonstrates causal relationships between gastroesophageal reflux disease (GERD) and atopic disorders (asthma and atopic dermatitis [AD]). Specifically, asthma is shown to be a causal risk factor for AD, and GERD could be a shared pathogenic mechanism for both asthma and AD.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Jung-A A. Woo, Maria Castano, Teresa R. Kee, Jordan Lee, Cynthia J. Koziol-White, Steven S. An, Donghwa Kim, David E. Kang, Stephen B. Liggett
Summary: TAS2Rs are bitter taste receptors expressed on human airway smooth muscle (HASM) cells. Activation of TAS2Rs promotes airway relaxation through severing of F-actin. This destabilization of actin is due to cofilin dephosphorylation, which is mediated by TAS2R-induced deactivation of LIM domain kinase. The understanding of this mechanism provides new insights into potential targets for bronchodilators.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2023)
Letter
Allergy
En Xu, Gaoyuan Cao, Zhi Yang, Yuanyue Zhang, Youwen Si, Kunal Singh, Joseph Jude, Steven S. An, Cynthia J. Koziol-White, Reynold A. Panettieri, Qi Yang
Article
Biochemistry & Molecular Biology
Eric B. Gebski, Vishal Parikh, Hong Lam, Nicholas Kim, Yury A. Bochkov, Gaoyuan Cao, Reynold A. Panettieri, Richard Kurten, James Gern, Steven S. An, Cynthia J. Koziol-White
Summary: Rhinovirus C15 attenuates agonist-induced bronchodilation, decreases efficacy of current therapeutics, and modulates components of relaxation pathways in airway smooth muscle.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Kazunori Sunadome, Alek G. G. Erickson, Delf Kah, Ben Fabry, Csaba Adori, Polina Kameneva, Louis Faure, Shigeaki Kanatani, Marketa Kaucka, Ivar Dehnisch Ellstroem, Marketa Tesarova, Tomas Zikmund, Jozef Kaiser, Steven Edwards, Koichiro Maki, Taiji Adachi, Takuya Yamamoto, Kaj Fried, Igor Adameyko
Summary: The developing skeleton produces mechanical tension that guides the directional outgrowth of skeletal muscles. Formation of oriented myofibrils is crucial in musculoskeletal development. The mechanisms that control myocyte orientation and fusion for muscle directionality in adults are still unknown.
NATURE COMMUNICATIONS
(2023)
Article
Physics, Applied
Sebastian J. Mueller, Ben Fabry, Stephan Gekle
Summary: Bioprinting of living cells can lead to shape deformations, affecting cell survival and functionality. Extensional stresses occurring when cells leave the nozzle have been ignored. Lattice Boltzmann simulations and a finite-element based cell model were used to study cell deformation inside the nozzle and at its exit. Two simple methods were developed to predict maximum cell stress and approximate cell strains based on printing parameters.
PHYSICAL REVIEW APPLIED
(2023)
Article
Biochemistry & Molecular Biology
Ajay P. Nayak, Elham Javed, Dominic R. Villalba, Yinna Wang, Henry P. Morelli, Sushrut D. Shah, Nicholas Kim, Rennolds S. Ostrom, Reynold A. Panettieri, Steven S. An, Dale D. Tang, Raymond B. Penn
Summary: Prostaglandin E2 has various physiological effects on multiple airway cells through its actions on different E-type prostanoid receptor subtypes. The EP2 and EP4 receptors, in particular, play a role in regulating the contraction and signaling of airway smooth muscle. However, the effectiveness of subtype-specific agonists in relaxing the muscle under different stimulation conditions varies, suggesting the involvement of compartmentalized signaling mechanisms.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2023)
Meeting Abstract
Oncology
Mikaela M. Mallin, Nicholas Kim, Mohammad Ikbal Choudhury, SeJong Lee, Steven S. An, Sean X. Sun, Konstantinos Konstantopoulos, Sarah R. Amend, Kenneth J. Pienta