Article
Neurosciences
Ruijie Li, Sibo Wang, Jing Lyu, Ke Chen, Xiaxin Sun, Junjie Huang, Pei Sun, Susu Liang, Min Li, Mengke Yang, Hongbang Liu, Shaoqun Zeng, Xiaowei Chen, Longhui Li, Hongbo Jia, Zhenqiao Zhou
Summary: This study introduces a high-speed two-photon microscope based on a custom-built acousto-optic deflector (AOD) that provides high temporal resolution and versatility. By combining AOD with resonance-galvo (RS) scanning, the microscope allows for stepwise magnification from population view to subcellular view with high spatial and temporal resolution. The results demonstrate that this method can effectively measure the velocity of neuronal activities and blood flow.
Article
Multidisciplinary Sciences
Guanghan Meng, Jian Zhong, Qinrong Zhang, Justin S. J. Wong, Jianglai Wu, Kevin K. Tsia, Na Ji
Summary: Understanding blood flow dynamics is crucial for studying the function of blood vessels. Current methods lack the resolution to monitor blood flow at a cellular level in large vessels. However, using a fast line-scanning module, researchers were able to achieve high-resolution imaging of blood flow in the cortex of awake mice. This breakthrough allowed them to measure cerebral blood flow and observe pulsatile blood flow patterns.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Conghao Wang, Qinmiao Chen, Huilan Liu, Runlong Wu, Xiong Jiang, Qiang Fu, Zhe Zhao, Ye Zhao, Yuqian Gao, Bosong Yu, Hongchen Jiao, Aimin Wang, Shumin Xiao, Lishuang Feng
Summary: Miniature two-photon microscopy is a powerful technique for investigating brain activity in freely moving animals. This study successfully addressed the challenges of probe weight and animal behavior constraints by employing metalenses, and demonstrated two-photon imaging of mouse brain structures.
Review
Chemistry, Analytical
Vinayak Juvekar, Hyo Won Lee, Dong Joon Lee, Hwan Myung Kim
Summary: Two-photon microscopy is an important tool for deep-tissue imaging, providing high-resolution fluorescence images beyond millimeter depths. Recent research has focused on developing innovative strategies and probes for quantitative analysis using two-photon fluorescence.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Wei Yan, Yangrui Huang, Luwei Wang, Jin Li, Yong Guo, Zhigang Yang, Junle Qu
Summary: This study demonstrates a multi-color two-photon imaging method with single-wavelength excitation. The problem of spectral crosstalk is effectively solved by selecting suitable filter combinations and applying image subtraction. The results show that two-color and three-color two-photon imaging can be achieved using a single femtosecond laser.
Review
Immunology
Tuula Penate Medina, Jan Philip Kolb, Gereon Huttmann, Robert Huber, Oula Penate Medina, Linh Ha, Patricia Ulloa, Naomi Larsen, Arianna Ferrari, Magdalena Rafecas, Mark Ellrichmann, Mariya S. Pravdivtseva, Mariia Anikeeva, Jana Humbert, Marcus Both, Jennifer E. Hundt, Jan-Bernd Hovener
Summary: This article reviews the recent advances in imaging technologies and their potential applications in the medical field, focusing on the development of imaging in precision medicine for inflammation. It also analyzes the challenges and opportunities faced by imaging technologies, showcasing some current or near-clinical applications as examples.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Luqman Khan, Rick van Lanen, Govert Hoogland, Olaf Schijns, Kim Rijkers, Dimitrios Kapsokalyvas, Marc van Zandvoort, Roel Haeren
Summary: The review highlighted the application of two-photon imaging (2PI) in epilepsy research, showcasing its ability to evaluate pathomechanisms associated with epileptic seizures, including propagation models, cellular changes, and neurovascular alterations. The findings suggest that 2PI is a valuable imaging technique in advancing our understanding of epilepsy pathophysiology.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Multidisciplinary
M. Marini, A. Nardini, R. Martinez Vazquez, C. Conci, M. Bouzin, M. Collini, R. Osellame, G. Cerullo, B. S. Kariman, M. Farsari, E. Kabouraki, M. T. Raimondi, G. Chirico
Summary: Non-linear excitation microscopy has advantages over conventional confocal techniques for in-vivo imaging, but tissue penetration can still be problematic. This study develops and tests fibroblast cell culture plano-convex microlenses for non-linear imaging of biological tissue. The microlenses can be used individually or in an array, and they provide magnified fluorescence images without substantially affecting the signal-to-noise ratio. These results pave the way for optical in-vivo inspection of biological processes using implanted micro-optics.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Biochemical Research Methods
Po-Yen Lin, Sheng-Ping L. Hwang, Chi-Hon Lee, Bi-Chang Chen
Summary: By incorporating axicon imaging into LSFM, the depth of field is extended, allowing for faster three-dimensional imaging and capturing dynamic changes in physiological processes.
JOURNAL OF BIOMEDICAL OPTICS
(2021)
Letter
Oncology
Shrikant B. Mali
Summary: Intravital microscopy and optical coherency tomography are powerful imaging tools that have revolutionized cancer diagnosis and therapies. They allow for real-time observation of biological processes in vivo, providing insights into tumor behaviors. Recent developments in techniques for observing deep tissues have improved the detection of subcellular events and made these technologies indispensable in basic sciences and drug development.
Article
Biochemical Research Methods
Weijian Zong, Runlong Wu, Shiyuan Chen, Junjie Wu, Hanbin Wang, Zhe Zhao, Guoqing Chen, Rui Tu, Danlei Wu, Yanhui Hu, Yangyang Xu, Yao Wang, Zhuoli Duan, Haitao Wu, Yunfeng Zhang, Jue Zhang, Aimin Wang, Liangyi Chen, Heping Cheng
Summary: Our miniature two-photon microscope, equipped with an axial scanning mechanism and a long-working-distance miniature objective, enables multi-plane imaging within a small animal's body, aiding in deciphering neuronal mechanisms.
Editorial Material
Biochemical Research Methods
Jerome A. Lecoq, Roman Boehringer, Benjamin F. Grewe
Summary: New three-photon miniature microscopes enable the study of neuronal networks in the deep regions of the brains of behaving animals.
Article
Multidisciplinary Sciences
Giovanni Barbera, Rachel Jun, Yan Zhang, Bo Liang, Yun Li, Da-Ting Lin
Summary: In this work, a lightweight one-photon microscope capable of imaging at different focal depths is presented. The focal plane can be dynamically changed by modulating the pulse width of the control signal, enabling the switching of focal plane between frames. Experimental results showed an increase in the number of imaged neurons compared to single plane imaging.
SCIENTIFIC REPORTS
(2022)
Review
Plant Sciences
Yoko Mizuta
Summary: Two-photon excitation microscopy (2PEM) allows deep imaging of living tissues with reduced autofluorescence compared to confocal microscopy, achieving higher resolution. Fluorescent proteins with long emission wavelengths, such as orange-red fluorescent proteins, are suitable for high-quality two-photon live imaging in plants.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Biochemical Research Methods
Bowen Wei, Chenmao Wang, Zongyue Cheng, Baoling Lai, Wen-Biao Gan, Meng Cui
Summary: COMPACT allows imaging of large volumes in the brain through a periscope-like probe inside a glass capillary, enabling observation of the dynamics of neuronal structure and function in different regions. This technology provides a valuable tool for understanding the neural circuit mechanisms underlying behavior.
Article
Biology
Aniruddha Das, Kevin Murphy, Patrick J. Drew
Summary: This article reviews the systemic and local non-neural contributions to haemodynamic signals during resting-state fMRI, emphasizing the links between these vascular fluctuations and brain physiology. It also discusses tools developed to correct for these fluctuations and how fMRI measurements can be utilized to gain valuable information about neurovascular health and internal brain states.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2021)
Article
Optics
Shubham Mirg, Haoyang Chen, Kevin L. Turner, Kyle W. Gheres, Jinyun Liu, Bruce J. Gluckman, Patrick J. Drew, Sri-Rajasekhar Kothapalli
Summary: This article proposes a transparent ultrasound transducer (TUT) based on lithium niobate as a cranial window for thinned mouse skull, which can be used for optical resolution photoacoustic microscopy (OR-PAM) imaging and other applications.
Editorial Material
Multidisciplinary Sciences
Giorgio A. Ascoli, Bing-Xing Huo, Partha P. Mitra
Article
Neurosciences
Qingguang Zhang, Kevin L. Turner, Kyle W. Gheres, Md Shakhawat Hossain, Patrick J. Drew
Summary: This article discusses the importance of monitoring behavioral state in imaging experiments using unanesthetized rodents and describes how to incorporate detailed behavioral and physiological measurements. The effects of movements and sleep-related signals on brain hemodynamics and electrophysiological signals are summarized, with guidance on integrating these measurements into functional brain imaging and electrophysiology. The article provides a how-to guide on monitoring and interpreting physiological signals in awake behaving mice for neurovascular coupling experiments, aiming to facilitate neuroimaging in awake animal models.
Correction
Neurosciences
Aaron T. Winder, Christina Echagarruga, Qingguang Zhang, Patrick J. Drew
NATURE NEUROSCIENCE
(2022)
Article
Neurosciences
Ravi Teja Kedarasetti, Patrick J. Drew, Francesco Costanzo
Summary: The movement of fluid in and out of the brain plays a vital role in clearing metabolic waste. This research investigates the mechanisms behind fluid movement and suggests that arteriolar dilations can drive fluid circulation in the brain, especially during sleep-like conditions. The study highlights the importance of convective flow in the extracellular space for waste clearance.
FLUIDS AND BARRIERS OF THE CNS
(2022)
Review
Hematology
Shubham Mirg, Kevin L. Turner, Haoyang Chen, Patrick J. Drew, Sri-Rajasekhar Kothapalli
Summary: Microcirculation plays a crucial role in maintaining tissue health, and photoacoustic imaging (PAI) is an effective technique for imaging microvascular networks and obtaining quantitative information about blood dynamics. PAI is of great clinical relevance in neurovascular, cardiovascular, ophthalmic, and cancer research fields. However, there are still challenges and limitations that need to be addressed in PAI.
Review
Physiology
Qingguang Zhang, William D. Haselden, Serge Charpak, Patrick J. Drew
Summary: Oxygen is crucial for neural metabolism, but the brain often has more oxygen than it needs. Changes in respiration rate, tied to the arousal state of the brain and cognition, can dynamically increase oxygen levels. The effects of these oxygenation changes on neural dynamics related to attention and arousal are still uncertain.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2023)
Article
Cell Biology
Yuan-ting Wu, Hannah C. Bennett, Uree Chon, Daniel J. Vanselow, Qingguang Zhang, Rodrigo Munoz-Castarieda, Keith C. Cheng, Pavel Osten, Patrick J. Drew, Yongsoo Kim
Summary: This study comprehensively defines the relationships between the cerebrovasculature, capillary pericytes, and different neuronal cell types using mapping methods. The results show regional variations in vasculature and pericyte densities, which correlate with energy-demanding neuronal subtypes.
Review
Neurosciences
Patrick J. Drew
Summary: In the brain, increases in neural activity lead to changes in local blood flow through neurovascular coupling. However, the traditional explanation that increased blood flow supplies the metabolic needs of active neurons is inconsistent with a large body of evidence. Neurovascular coupling is irregular or absent in many brain regions and states, and increased respiration can improve brain oxygenation without changes in blood flow. Simulation studies suggest that low blood flow areas are inevitable due to the brain's vascular architecture and cannot be eliminated by functional hyperemia. This article discusses potential alternative functions of neurovascular coupling such as supplying oxygen for neuromodulator synthesis, regulating brain temperature, signaling to neurons, optimizing cerebral vascular structure, accommodating non-Newtonian blood flow, and driving cerebrospinal fluid circulation.
TRENDS IN NEUROSCIENCES
(2022)
Article
Neurosciences
Kevin L. Turner, Kyle W. Gheres, Patrick J. Drew
Summary: Arousal state has significant impact on neural activity and blood vessel dynamics in the cortex. The relationship between pupil diameter, blink rate, neural activity, and blood volume in the somatosensory cortex was investigated in male and female mice. Pupil diameter was smaller during sleep and showed coherence with gamma-band power and blood volume. The correlation varied with arousal state and blinking was associated with changes in arousal and blood volume. Eye metrics could accurately determine the arousal state of the mice.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Neurosciences
Qingqing Zhang, Samuel R. Cramer, Kevin L. Turner, Thomas Neuberger, Patrick J. Drew, Nanyin Zhang
Summary: In this study, it was found that the multi-phase blood-oxygen-level dependent (BOLD) response observed in the visual cortices of unanesthetized rats during decreased illumination can be better explained by the high-frequency neuronal signal rather than non-neuronal physiological factors. The phases of the BOLD response were found to be reproducible and were not simply a result of the periodic stimulation structure.
Article
Multidisciplinary Sciences
Thanh Tan Vo, Geun Ho Im, Kayoung Han, Minah Suh, Patrick J. Drew, Seong-Gi Kim
Summary: In this study, the hemodynamic responses elicited by optogenetic stimulation of PV interneurons were investigated using electrophysiology, fMRI, OIS, and pharmacological applications. The findings showed that activation of PV neurons induced an early vasoconstrictive response and a later ultraslow vasodilation response. The ultraslow response was mediated by neuropeptide substance P released from PV neurons and was dependent on the brain state.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biology
Kyle W. W. Gheres, Hayreddin S. S. Unsal, Xu Han, Qingguang Zhang, Kevin L. L. Turner, Nanyin Zhang, Patrick J. J. Drew
Summary: This study found that sensory stimulation in neonatal mice induces a small increase in blood volume, followed by a large decrease. The arousal state of the mice, especially sleep, plays a dominant role in regulating neurovascular coupling, obscuring the sensory-evoked changes. The findings highlight the importance of considering sleep-related vascular changes in hemodynamic measures.
COMMUNICATIONS BIOLOGY
(2023)
Article
Neurosciences
Qingqing Zhang, Samuel R. Cramer, Zilu Ma, Kevin L. Turner, Kyle W. Gheres, Yikang Liu, Patrick J. Drew, Nanyin Zhang
Summary: The study reveals that ongoing brain-wide activity plays a significant role in the cross-trial variability of task-based functional magnetic resonance imaging (fMRI). Removing this ongoing activity can reduce the variability of the fMRI response and improve its coherence with neuronal activity.
Article
Neurosciences
Jose Sanchez-Bornot, Roberto C. Sotero, J. A. Scott Kelso, Ozguer Simsek, Damien Coyle
Summary: This study proposes a multi-penalized state-space model for analyzing unobserved dynamics, using a data-driven regularization method. Novel algorithms are developed to solve the model, and a cross-validation method is introduced to evaluate regularization parameters. The effectiveness of this method is validated through simulations and real data analysis, enabling a more accurate exploration of cognitive brain functions.