期刊
JOURNAL OF CELLULAR BIOCHEMISTRY
卷 112, 期 10, 页码 2891-2901出版社
WILEY
DOI: 10.1002/jcb.23205
关键词
PERIODONTAL LIGAMENT FIBROBLASTS; MECHANICAL FORCES; ALVEOLAR BONE REMODELING; TUMOR NECROSIS FACTOR-alpha; OSTEOCLASTOGENESIS; LYMPHOCYTES
资金
- Ministry for Health, Welfare & Family Affairs, Republic of Korea [A084283]
- Korea Health Promotion Institute [A084283] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Periodontal ligament fibroblasts (PLF) sense and respond to mechanical stimuli and participate in alveolar bone resorption during orthodontic treatments. This study examined how PLF influence osteoclastogenesis from bone marrow-derived macrophages (BMM) after application of tension or compression force. We also investigated whether lymphocytes could be a primary stimulator of osteoclastic activation during alveolar bone remodeling. We found that mechanical forces inhibited osteoclastic differentiation from BMM in co-cultures with PLF, with PLF producing predominantly osteoprotegerin (OPG) rather than receptor activator of nuclear factor-kappaB (NF-kappa B) ligand (RANKL). In particular, PLF increased the expression of tumor necrosis factor (TNF)-alpha in response to compression. Additional experiments showed the presence of CD4- and B220-positive cells with a subsequent increase in tartrate-resistant acid phosphatase (TRAP)-positive cells and RANKL expression only at the compression side of the force-subjected periodontal tissues. Exogenous TNF-alpha increased the number of TRAP-positive cells and pit formation in the co-cultures of BMM with Jurkat, but not with BJAB cells and this effect was almost completely inhibited by antibodies to TNF-alpha or TNF receptor. Collectively, the current findings suggest that PLF secrete relatively higher levels of TNF-alpha at the compression side than at the tension side and this imbalance leads to RANKL expression by activating CD4+ T cells, thereby facilitating bone resorption during orthodontic tooth movement. J. Cell. Biochem. 112: 2891-2901, 2011. (C) 2011 Wiley-Liss, Inc.
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