Article
Biochemistry & Molecular Biology
Ting-Wei Kao, Arthur Chiou, Keng-Hui Lin, Yi-Shiuan Liu, Oscar Kuang-Sheng Lee
Summary: Research shows that mechanical stimulation affects the intracellular viscoelasticity of human mesenchymal stem cells, with cells cultured in rigid matrix displaying higher elastic and viscous moduli. This effect remains consistent throughout the differentiation process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Laidi Wu, Zhixin Liu, Li Xiao, Mi Ai, Yingguang Cao, Jing Mao, Ke Song
Summary: This review comprehensively elucidates the specific roles of Gli1+ MSCs in craniofacial bone osteogenesis and tissue repair, offering new insights into bone regeneration therapy.
Article
Multidisciplinary Sciences
Sook-Kyoung Heo, Yunsuk Choi, Yoo Kyung Jeong, Lan Jeong Ju, Ho-Min Yu, Do Kyoung Kim, Hye Jin Seo, Yoo Jin Lee, Jaekyung Cheon, SuJin Koh, Young Joo Min, Eui-Kyu Noh, Jae-Cheol Jo
Summary: Osteoporosis is a progressive skeletal disease affecting millions worldwide, currently treated with drugs that have severe side effects. Cell replacement therapy using mesenchymal stem cells may be a future treatment strategy. This study found that LIGHT can enhance osteogenesis of hBM-MSCs, possibly through WNT/β-catenin pathway activation.
Article
Medicine, Research & Experimental
Leiluo Yang, Qing Li, Junhong Zhang, Pengcheng Li, Pingjiang An, Chunqing Wang, Pingsheng Hu, Xue Zou, Xiaowei Dou, Lixin Zhu
Summary: The study investigated the role of Wnt7a in bone formation and demonstrated its key role in MSC differentiation into osteoblasts, offering a potential therapeutic target for bone-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Review
Endocrinology & Metabolism
Soichiro Sonoda, Takayoshi Yamaza
Summary: Systemic transplantation of MSCs is an effective treatment for osteopenia, but the mechanism of action is unclear. Donor MSCs only have a limited presence in the recipient target tissue, suggesting that their direct contribution may not be significant. However, the secretomes and extracellular vesicles (EVs) released from donor MSCs play a key role in disease treatment. This review discusses the contribution of MSC-EVs to bone recovery and introduces a novel mechanism of action of SHED-EVs in osteopenia. It is found that MSC-EVs can rejuvenate the microenvironment and recover bone density by enhancing telomerase activity in recipient BMMSCs. Future challenges in developing reproducible MSC-EV therapy in osteopenia are also addressed.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Cell & Tissue Engineering
Zhaohua Wang, Si Wen, Meiqi Zhong, Ziming Yang, Wei Xiong, Kuo Zhang, Shude Yang, Huizheng Li, Shu Guo
Summary: Bone has limited capacity to repair critical-sized bone defects, but stem cells, particularly mesenchymal stem cells (MSCs), show promise in enhancing bone regeneration. Epigenetic modification, including nucleic acids methylation, histone modification, and non-coding RNAs, plays a crucial role in determining MSCs fate and differentiation. Understanding the epigenetic mechanisms of MSCs can improve stem cell activity and function. This review highlights the potential of harnessing epigenetic modification of MSCs for treating bone defects and promoting bone regeneration, providing therapeutic targets for bone-related diseases.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Sepideh Saghati, cigir Biray Avci, Ayla Hassani, Sajed Nazifkerdar, Hassan Amini, Solmaz Saghebasl, Mahdi Mahdipour, Behnaz Banimohamad-Shotorbani, Atieh Rezaei Namjoo, Fateme Nazary Abrbekoh, Reza Rahbarghazi, Hamid Tayefi Nasrabadi, Ali Baradar Khoshfetrat
Summary: This study investigated the osteogenic properties of phenolated alginate hydrogel containing collagen and nano-hydroxyapatite on human mesenchymal stem cells. The results showed that the combination of alginate/collagen/nano-hydroxyapatite had suitable mechanical features and promoted cell viability, osteoblast differentiation, and new bone formation through involving the Wnt signaling pathway.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Multidisciplinary Sciences
Nishant Verma, Todd Le, Jonah Mudge, Peter J. Nicksic, Lillian Xistris, Maisha Kasole, Andrew J. Shoffstall, Samuel O. Poore, Kip A. Ludwig, Aaron M. Dingle
Summary: Noninvasive electronic bone growth stimulators (EBGSs) have been used clinically for decades, but systematic reviews show inconsistent and limited efficacy. Studies in small animals suggest promising efficacy when the stimulation electrode is closer to the fracture site, but this has not been replicated in large animals or humans. The weaker electric fields reaching the fracture site when scaling from small animals to large animals and humans may explain this disparity.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Yoon Young Go, Sung-won Chae, Jae-Jun Song
Summary: Human chorionic membrane extracts (CME) enhance osteogenesis by providing an osteoconductive environment for osteoblasts. CME contains various osteogenesis-related stimulators and triggers osteogenic differentiation through specific cellular signaling pathways.
CELL PROLIFERATION
(2022)
Article
Cell Biology
Dong Hoon Kwak, Ji Hye Park, Eul Sig Choi, Seong Hyun Park, Seo-Yeon Lee, Seoul Lee
Summary: This study analyzed the role of gangliosides in the osteogenesis of Lmna dysfunctional MSCs. The results showed that ganglioside GD1a played an important role in enhancing osteogenic differentiation in Lmna-mutated MSCs.
Article
Cell Biology
Asim Cengiz Akbulut, Grzegorz B. Wasilewski, Nikolas Rapp, Francesco Forin, Heike Singer, Katrin J. Czogalla-Nitsche, Leon J. Schurgers
Summary: This study generated mature osteoblasts from iPSCs using a stable expandable MSC intermediate, and investigated the effects of MK-7 on cell differentiation. The findings suggest that MK-7 promotes osteoblast maturation and increases osteogenic differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Hai-Bo Huang, Hai-Tao Luo, Na-Na Wei, Miao-Ling Liu, Fei He, Wei Yang, Jun Dong, Xiao-Fei Yang, Fu-Rong Li
Summary: This study revealed the landscape of lineage-specific circRNAs during adipo-osteogenesis of mesenchymal stem cells (MSCs) through integrative analysis. It identified circ-CRLF1 as a potential therapeutic target for skeleton-related diseases, providing new insights for their treatment.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Engineering, Biomedical
Qian Wang, Lei Xu, Regine Willumeit-Roemer, Berengere J. C. Luthringer-Feyerabend
Summary: The study reveals that magnesium and Mg-10Gd can modulate osteogenic differentiation through various pathways, including enhancing secretion of bone formation-related signaling complexes and reducing inflammatory factor secretion in macrophages and cocultures.
ACTA BIOMATERIALIA
(2021)
Article
Pharmacology & Pharmacy
Jeevithan Elango
Summary: This study developed a novel hydrogel system using collagen, chitosan, and VEGF composites for culturing MSCs and investigated their ability for osteogenic differentiation and mineral deposition. The hydrogel loaded with 100 ng/mL VEGF showed significant support for MSC proliferation, fibrillary filament structure, mineralization, alkaline phosphatase, and osteogenesis compared to other hydrogels and control. The hydrogel with VEGF could be used as an appropriate system to culture stem cells for bone and dental regeneration.
Article
Biophysics
Ee-Seul Kang, Huijung Kim, Yoojoong Han, Yeon-Woo Cho, Hyungbin Son, Zhengtang Luo, Tae-Hyung Kim
Summary: Graphene derivatives show promise for stem-cell-based regenerative therapies, especially in bone regeneration. A graphene oxide-based hybrid platform was developed to guide the osteogenesis of human adipose-derived mesenchymal stem cells effectively. The GOHP exhibited efficiency comparable to high-quality CVD-grown graphene in inducing osteogenesis by upregulating specific genes.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)