Article
Cell Biology
Qiudong Yang, Wenhua Zhao, Yuyi Chen, Yue Chen, Jiali Shi, Ran Qin, Hua Wang, Ruixia Wang, Hua Yuan, Wen Sun
Summary: The study reveals that the RelA/miR-30a/NLRP3 signaling axis is involved in rheumatoid arthritis by regulating the NLRP3 inflammasome in macrophages.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Sarah Venus, Kaba Tandjigora, Eckhard Jankowsky
Summary: The protein K7 from vaccinia virus is shown to inhibit the biochemical activities and cellular functions of DDX3X by binding to its N-terminus, restraining its association with stress granules.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Immunology
Nadia Jeremiah, Hermine Ferran, Konstantina Antoniadou, Kevin De Azevedo, Jovan Nikolic, Mathieu Maurin, Philippe Benaroch, Nicolas Manel
Summary: A transcriptional rheostat orchestrated by RELA controls the expression of type I/III interferon in T cells, conferring them with innate-like abilities. Despite intact cGAS-STING signaling, CD4(+) T cells exhibit weaker IFN-I/III responses compared to dendritic cells or macrophages. The lysine residues in RELA regulate the IFN-I/III response in CD4(+) T cells at baseline and in response to STING stimulation.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Plant Sciences
Chi Xu, Zhanhui Zhang, Juan He, Yongsheng Bai, Jie Cui, Lin Liu, Jihua Tang, Guiliang Tang, Xuemei Chen, Beixin Mo
Summary: RCF1 is shown to play a role in pri-miRNA processing and splicing, as well as pre-mRNA splicing in Arabidopsis thaliana. A mutant with miRNA biogenesis defects was identified, and it was found to be caused by a recessive point mutation in RCF1 (rcf1-4). RCF1 promotes D-body formation and facilitates the interaction between pri-miRNAs and HYL1, and rcf1-4 exhibits a global splicing defect in intron-containing pri-miRNAs and pre-mRNAs. This study uncovers the importance of RCF1 in miRNA biogenesis and RNA splicing in Arabidopsis.
Article
Biochemistry & Molecular Biology
Boris Bonaventure, Antoine Rebendenne, Ana Luiza Chaves Valadao, Mary Arnaud-Arnould, Segolene Gracias, Francisco Garcia de Gracia, Joe McKellar, Emmanuel Labaronne, Marine Tauziet, Valerie Vivet-Boudou, Eric Bernard, Laurence Briant, Nathalie Gros, Wassila Djilli, Valerie Courgnaud, Hugues Parrinello, Stephanie Rialle, Mickael Blaise, Laurent Lacroix, Marc Lavigne, Jean-Christophe Paillart, Emiliano P. Ricci, Reiner Schulz, Nolwenn Jouvenet, Olivier Moncorge, Caroline Goujon
Summary: DDX42 has been identified as an intrinsic antiviral inhibitor, restricting HIV-1 and other RNA viruses. It does not impact the replication of several negative-strand RNA viruses, suggesting a specific mode of action on target cells.
Article
Biology
Yaqiong Jin, Jin Shi, Huanmin Wang, Jie Lu, Chenghao Chen, Yongbo Yu, Yaru Wang, Yeran Yang, Dong Ren, Qi Zeng, Xin Ni, Yongli Guo
Summary: The study found that the DDX1 gene polymorphism rs72780850 is associated with an increased risk of NB, especially in aggressive cases. This association may be due to the regulation of DDX1 gene expression affecting tumor risk. Additionally, the molecular mechanism of the rs72780850 polymorphism leading to increased NB risk may be related to the binding affinity of the MAX transcription factor.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Paul T. Winnard, Farhad Vesuna, Venu Raman
Summary: DDX3X, a multifunctional protein utilized by various viruses, plays a crucial role in viral metabolism. Modulating the functions of DDX3 during viral infections could effectively suppress viral production and have a significant impact on safely and efficiently subduing a broad spectrum of viral infections.
ANTIVIRAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Jun-Xiu Wang, En-Bo Ma, Jian-Zhen Zhang, Shu-Ping Xing
Summary: Cellular and molecular mechanisms play a key role in maintaining midgut homeostasis, with LmDDX47 acting as an important regulator by controlling 18S rRNA synthesis and protein modification levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Hugo L. de Araujo, Beatriz A. Picinato, Alan P. R. Lorenzetti, Nisansala S. Muthunayake, I. W. Rathnayaka-Mudiyanselage, Naara M. dos Santos, Jared Schrader, Tie Koide, Marilis V. Marques
Summary: The absence of the RNA helicase RhlB in Caulobacter crescentus causes important changes in gene expression and cell physiology. These changes are likely due, at least in part, to inefficient RNA processing by the RNA degradosome, especially at low-temperature conditions.
MICROBIOLOGY SPECTRUM
(2023)
Article
Plant Sciences
Lili Zhang, Yanjie Xu, Xuening Liu, Meizhu Qin, Shenglan Li, Tianhua Jiang, Yingjie Yang, Cai-Zhong Jiang, Junping Gao, Bo Hong, Chao Ma
Summary: The chrysanthemum DEAD-box RNA helicase gene CmRH56 plays a role in modulating rhizome outgrowth in response to drought stress by repressing gibberellin biosynthesis.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Multidisciplinary Sciences
Shringar Rao, Cynthia Lungu, Raquel Crespo, Thijs H. Steijaert, Alicja Gorska, Robert-Jan Palstra, Henrieke A. B. Prins, Wilfred van Ijcken, Yvonne M. Mueller, Jeroen J. A. van Kampen, Annelies Verbon, Peter D. Katsikis, Charles A. B. Boucher, Casper Rokx, Rob A. Gruters, Tokameh Mahmoudi
Summary: This study demonstrates the selective induction of cell death in latent HIV-1-infected cells by DDX3 inhibitors, and provides evidence for the reduction of the inducible HIV-1 reservoir in CD4+ T cells from PLWHIV. The pharmacological targeting of DDX3 shows promise in reversing HIV-1 latency and eliminating the latent reservoir.
NATURE COMMUNICATIONS
(2021)
Article
Genetics & Heredity
Denise S. Whitford, Brendan T. Whitman, George W. Owttrim
Summary: The study conducted a phylogenetic analysis of DEAD-box RNA helicases in cyanobacteria, revealing three distinct clades and limited distribution across cyanobacterial diversity. Additionally, extensive conservation of gene synteny surrounding the rimO?crhR operon was observed, indicating a role in the maintenance of photosynthesis.
MICROBIAL GENOMICS
(2021)
Article
Multidisciplinary Sciences
Akira Nakazato, Mai Mochizuki, Rie Shibuya-Takahashi, Haruna Fujimori, Keitaro Fujii, Satoshi Saijoh, Shinkichi Morita, Tomoko Yamazaki, Takayuki Imai, Ikuro Sato, Kennichi Satoh, Kazunori Yamaguchi, Kazuo Sugamura, Jun Yasuda, Kazuto Matsuura, Hideo Shojaku, Yukinori Asada, Keiichi Tamai
Summary: This study found that RELA, a subunit of nuclear factor kappaB, is critical for the transcription of CD271 in hypopharyngeal cancer cells. RELA promotes CD271 transcription in squamous cell carcinoma cell lines, but not in normal epithelium and neuroblastoma cell lines. The specific region + 957 to + 1138 within the CD271 promoter sequence is important for RELA binding.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Spyros Foutadakis, Eugenia Roupakia, Panagiotis Liakopoulos, Petros Kolovos, Evangelos Kolettas
Summary: Transcription Factors (TFs) are key regulators of gene expression, controlling aspects such as cell homeostasis, identity, and fate. NF-kappa B and E2F1 are important TFs involved in inflammation and cell cycle progression, respectively. This study compared their genomic binding profiles and found distinct preferences in the type of DNA elements they bind to. Additionally, NF-kappa B and E2F1 showed limited overlap in their binding sites and tended to bind to active chromatin even before stimulation. Furthermore, NF-kappa B was found to recruit E2F1 near pro-inflammatory genes following immune stimulation.
Article
Neurosciences
Anna Simankova, Norihisa Bizen, Sei Saitoh, Shinsuke Shibata, Nobuhiko Ohno, Manabu Abe, Kenji Sakimura, Hirohide Takebayashi
Summary: Ddx20 is essential for the terminal differentiation of oligodendrocytes and maintenance of myelin gene expression. Knockout of Ddx20 in mice led to abnormal behaviors, muscle weakness, and reduced lifespan, with significant reductions in mature oligodendrocytes and myelin-associated gene expression levels.
Review
Virology
Yu Chen, Qianyun Liu, Deyin Guo
JOURNAL OF MEDICAL VIROLOGY
(2020)
Letter
Medicine, General & Internal
Shibo Jiang, Zhengli Shi, Yuelong Shu, Jingdong Song, George F. Gao, Wenjie Tan, Deyin Guo
Article
Virology
Deyin Guo
Article
Cell Biology
Panpan Hou, Kongxiang Yang, Penghui Jia, Lan Liu, Yuxin Lin, Zibo Li, Jun Li, Shuliang Chen, Shuting Guo, Junyu Wu, Hong Peng, Weijie Zeng, Chunmei Li, Yingfang Liu, Deyin Guo
Summary: CCDC50 is a newly discovered autophagy receptor that negatively regulates the type I interferon signaling pathway by targeting K63-polyubiquitinated RLRs for autophagic degradation, which enhances viral resistance and survival rates in mouse models.
Article
Virology
Junwei Li, Mingyue Song, Deyin Guo, Yongxiang Yi
Article
Biochemistry & Molecular Biology
Yuxin Lin, Zibo Li, Yicheng Wang, Tian Tian, Penghui Jia, Yu Ye, Miao He, Zixiao Yang, Chunmei Li, Deyin Guo, Panpan Hou
Summary: This study identifies a novel autophagy cargo receptor, CCDC50, which negatively regulates NLRP3 inflammasome assembly and the release of related inflammatory factors by degrading NLRP3. CCDC50 deficiency results in upregulation of signaling pathways associated with autoinflammatory diseases and enhances gut inflammation response.
Review
Virology
Wenhao Wang, Xiaoxue Peng, Yunyun Jin, Ji-An Pan, Deyin Guo
Summary: The ongoing COVID-19 pandemic has led to severe public health crises and economic losses. Limited knowledge about the SARS-CoV-2 virus calls for more research. Reverse genetics systems, like viral infectious clones and replicons, provide powerful platforms for various viral research projects. The development of noninfectious replicons allows for safer operations in more widely available Biosafety Level 2 (BSL2) facilities. After the outbreak, multiple reverse genetics systems for SARS-CoV-2 have been developed, offering researchers various options for their studies.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Oncology
Jingshu Xiao, Jiaming Liang, Junjie Fan, Panpan Hou, Xiaodong Li, Haipeng Zhang, Kai Li, Lang Bu, Ping Li, Miao He, Yongheng Zhong, Liping Guo, Penghui Jia, Qiaoqiao Xiao, Junyu Wu, Hong Peng, Chunmei Li, Fan Xing, Deyin Guo
Summary: This study presents a promising strategy for the treatment of aggressive glioblastoma (GBM) by combining CDK4/6 inhibitors with oncolytic viruses (OV). CDK4/6 inhibition enhances the efficacy of OV by promoting autophagic degradation of MAVS, impairing antiviral responses, and amplifying oncolysis. Furthermore, CDK4/6 inhibitors and OV synergistically induce immuno-genic cell death and boost antitumor immunity, inhibiting tumor growth and prolonging survival in GBM models.
Letter
Cell Biology
Lihong Liu, Birong Zheng, Manhui Luo, Jie Du, Fang Yang, Chuanxin Huang, Zengyi Ma, Chunmei Li, Deyin Guo, Hong Peng
Article
Microbiology
Hongchang Dong, Wencheng Wu, Jingjing Li, Yilei Ma, Xiaomei Deng, Deyin Guo, Pei Xu
Summary: This study provides detailed insights into the signal transduction pathways of PML bodies in response to IFN and virus infection. It reveals the involvement of the constitutive component Sp100A in mediating these processes and highlights the importance of nuclear importation of Sp100A in activating essential antiviral genes.
Letter
Biochemistry & Molecular Biology
Liping Guo, Xue Liu, Yuchen Gu, Jinyan Jiang, Ziyue Yang, Qiuying Lv, Deyin Guo, Yang Yang, Hongzhou Lu, Jing Yuan
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Letter
Biochemistry & Molecular Biology
Qifan Zhou, Sidi Yang, Liu Cao, Yang Yang, Tiefeng Xu, Qishu Chen, Hongzhou Lu, Yingjun Li, Deyin Guo, Xumu Zhang
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Letter
Biochemistry & Molecular Biology
Xiangpeng Sheng, Fang Zhu, Hong Peng, Fan Yang, Yi Yang, Cong Yang, Zhen Wang, Wei Chen, Deyin Guo, Ronggui Hu
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Letter
Biochemistry & Molecular Biology
Lang Bu, Huan Wang, Ji-an Pan, Lang Chen, Fan Xing, Junyu Wu, Shun Li, Deyin Guo
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)
Letter
Biochemistry & Molecular Biology
Fan Xing, Jingshu Xiao, Junyu Wu, Jiaming Liang, Xiaoyu Lu, Liping Guo, Ping Li, Panpan Hou, Chunmei Li, Deyin Guo
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)