Article
Pharmacology & Pharmacy
Tal Berg, Ofri Doppelt-Flikshtain, Benjamin R. Coyac, Hadar Zigdon-Giladi
Summary: The aim of this study was to investigate the influence of Zoledronic Acid and soft tissue cells on osteogenic differentiation of mesenchymal stem cells (MSCs). The results showed that Zoledronic Acid significantly decreased the osteogenic differentiation of MSCs, but the addition of oral fibroblasts' conditioned medium could restore the osteogenic differentiation of MSCs. It was found that oral fibroblasts secrete proteins that enable MSCs' osteogenic differentiation in the presence of Zoledronic Acid.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Yoojin Seo, Min-Jung Kang, Hyung-Sik Kim
Summary: MSCs are used as cell therapeutics for immune disorders due to their immunoregulatory properties, but variation among cells from different donors and rapid clearance after transplantation limit their efficacy. Various bioengineering strategies, including genetic modification and physical engineering, have been suggested to improve the immunomodulatory functions of MSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Engineering, Biomedical
Calvin Chang, Jerry Yan, Zhicheng Yao, Chi Zhang, Xiaowei Li, Hai-Quan Mao
Summary: This review focuses on the bioactivity and secretome of mesenchymal stem cells, as well as the engineering approaches to tune the MSC secretome. It also discusses the recent advances in biomaterials-based therapeutic strategies for delivering MSCs and MSC-derived secretome in extracellular vesicles, along with their advantages and challenges.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Engineering, Environmental
Xianglin Fan, Jie Zhan, Xianmei Pan, Xu Liao, Wenjie Guo, Peier Chen, Hekai Li, Weijing Feng, Yanbin Cai, Minsheng Chen
Summary: Transplantation of stem cells with biomaterials is an effective method for treating degenerative diseases. Synthetic biomaterials, which mimic the native tissue microenvironment, have been extensively used in tissue engineering. However, it is challenging to develop synthetic materials that can mimic the dynamic processes of cell-mediated remodeling.
CHEMICAL ENGINEERING JOURNAL
(2022)
Review
Biochemistry & Molecular Biology
Li-Na Zhang, Di-Di Zhang, Lei Yang, Yu-Xuan Gu, Qiu-Ping Zuo, Hao-Yi Wang, Jia Xu, Dian-Xin Liu
Summary: Invasion and metastasis are key characteristics of malignant tumors, with epithelial-mesenchymal transition (EMT) recognized as the initial step. Cell fusion, particularly involving mesenchymal stromal/stem cells (MSCs), plays a critical role in cancer development and progression, potentially influencing tumor growth and metastasis.
Article
Dentistry, Oral Surgery & Medicine
Carina Lantmann Cabreira, Roberta Limeira Fulginiti, Patricia Sesterheim, Rosemary Sadami Arai Shinkai, Eduardo Rolim Teixeira
Summary: This study evaluated different molecular weight hyaluronic acids as potential matrices for tissue-engineered bone grafting, with results showing higher expression of growth factors in the high/low molecular weight group. Differentiated cells secreted fewer cytokines compared to undifferentiated cells, with IGF-1 showing the highest expression in the low molecular weight group.
CLINICAL ORAL INVESTIGATIONS
(2021)
Article
Medicine, Research & Experimental
Jin Qian, Bauer L. LeSavage, Kelsea M. Hubka, Chenkai Ma, Suchitra Natarajan, Joshua T. Eggold, Yiren Xiao, Katherine C. Fuh, Venkatesh Krishnan, Annika Enejder, Sarah C. Heilshorn, Oliver Dorigo, Erinn B. Rankin
Summary: Ovarian cancer-associated mesothelial cells play a critical role in promoting chemoresistance and stemness in ovarian cancer. The secreted factor osteopontin drives mesothelial-mediated chemoresistance and stemness, and therapeutic targeting of osteopontin may enhance platinum sensitivity in ovarian cancer.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Cell & Tissue Engineering
Nurul Dinah Kadir, Zheng Yang, Afizah Hassan, Vinitha Denslin, Eng Hin Lee
Summary: This study demonstrates that conditioning media of MSCs cultured on electrospun fiber sheets have improved secretome yield and profile compared to those generated from TCP, enhancing cell migration and proliferation, promoting chondrogenesis, mitigating inflammation, and protecting chondrocytes from apoptosis. Topographical cues from aligned or random fiber orientation also influence FAK and ERK signaling pathways in modulating MSC secretome production.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Materials Science, Multidisciplinary
Hao Ye, Yan Li, Jinze Li, Cong Cao, Yuxi Luo, Yihong Gong
Summary: Three-dimensional polyethylene terephthalate microfiber sponge (PET-Gel) as a cell carrier can promote the adhesion and proliferation of human umbilical cord mesenchymal stem cells (HUCMSCs). Material characterization results showed that PET-Gel has good coating stability, thermal stability, and mechanical properties, and is suitable for long-term in vitro culture of HUCMSCs. The paracrine function of cells on PET-Gel is nearly equivalent to that on 2D plates.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Karla Montalban-Hernandez, Cesar Casado-Sanchez, Jose Avendano-Ortiz, Jose Carlos Casalvilla-Duenas, Gloria C. Bonel-Perez, Julia Prado-Montero, Jaime Valentin-Quiroga, Roberto Lozano-Rodriguez, Veronica Terron-Arcos, Fatima Ruiz de la Bastida, Laura Cordoba, Fernando Laso-Garcia, Luke Diekhorst, Carlos del Fresno, Eduardo Lopez-Collazo
Summary: This study successfully generated a new hybrid cell population, named foam hybrid cell (FHC), through co-culture. The FHC exhibited enhanced motility, proliferation, immunomodulation properties, and maintained stemness features. This finding is of great significance for stem cell therapy and regenerative medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Sree Samanvitha Kuppa, Hyung Keun Kim, Ju Yeon Kang, Seok Cheol Lee, Jong Keun Seon
Summary: Osteoarthritis is a low-grade inflammatory disorder of the joints, but there are limited treatment options currently available. This article explores the use of mesenchymal stem cells and their secreted factors for intervention in osteoarthritis, with a focus on the polarization of macrophages to reduce inflammation and symptoms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Afsaneh Asgari Taei, Pariya Khodabakhsh, Sanaz Nasoohi, Maryam Farahmandfar, Leila Dargahi
Summary: It is well known that the therapeutic effects of transplanted mesenchymal stem cells (MSCs) in ischemic stroke are not due to cell replacement, but rather the secretion of bioactive molecules. The secretome of MSCs, which includes growth factors, cytokines, and extracellular vesicles, plays a crucial role in neuroprotection. The application of secretome derivatives, such as conditioned medium or purified extracellular vesicles, has shown significant advantages over MSC transplantation in stroke treatment.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Xingliang Dai, Yuxuan Shao, Xuefeng Tian, Xiaoyan Cao, Lei Ye, Peng Gao, Hongwei Cheng, Xianwen Wang
Summary: Cell fusion between GSCs and MSCs in 3D-bioprinted models promotes the progression of glioma by enhancing the malignant properties of the fused cells.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Pharmacology & Pharmacy
Rui Alvites, Mariana Branquinho, Ana C. Sousa, Bruna Lopes, Patricia Sousa, Ana Colette Mauricio
Summary: Mesenchymal Stem/Stromal Cells (MSCs) have high clinical value and have been extensively studied. Their immunomodulatory influence occurs through both direct and paracrine routes, but the mechanisms are not fully understood. This review summarizes the immunoregulatory capacity of MSCs and their secretion products, with a focus on migration, homing, direct cell action, and paracrine activity, and explores related techniques.
Article
Cell & Tissue Engineering
Christopher L. Haga, Cori N. Booker, Ana Carvalho, Siddaraju Boregowda, Donald G. Phinney
Summary: Despite clinical testing, MSC-based therapies are not effective due to lack of predictive biomarkers. TWIST1 predicts inter-donor differences in MSC quality attributes. The present work identified TWIST1 targets related to ECM structure, skeletal and circulatory system development, interferon gamma signaling, and inflammation, which indicates the mechanistic link between stem/progenitor and paracrine activities of MSCs. The findings provide potential genes for further studies.