Article
Cell Biology
Oliver H. Voss, Daniel Arango, Justin C. Tossey, Miguel A. Villalona Calero, Andrea Doseff
Summary: Apigenin sensitizes primary lung cancer cells to TRAIL-induced apoptosis through reprogramming alternative splicing of key TRAIL/DISC components and directly binding heat shock protein 70 to promote cell death. These findings emphasize the synergies between diet and cancer treatments, providing new avenues for improved cancer therapies.
CELL DEATH & DISEASE
(2021)
Review
Immunology
Ehsan Razeghian, Wanich Suksatan, Heshu Sulaiman Rahman, Dmitry O. Bokov, Walid Kamal Abdelbasset, Ali Hassanzadeh, Faroogh Marofi, Mahboubeh Yazdanifar, Mostafa Jarahian
Summary: TRAIL, as an immune cytokine, has the ability to selectively eliminate tumor cells but faces resistance issues. Studies have shown that overcoming TRAIL resistance can be achieved through combined treatment with other antitumor agents, the utilization of human MSCs and NPs for TRAIL delivery. These approaches show promise in enhancing the therapeutic efficacy and survival rate in cancer treatment.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
K. M. A. Zinnah, Sang-Youel Park
Summary: The study demonstrated the mechanism behind the synergistic anticancer effect of amitriptyline and TRAIL, showing that amitriptyline increases TRAIL-induced apoptosis by upregulating death receptors DR4 and DR5. Inhibition of autophagy by amitriptyline was also shown to enhance DR4 and DR5 expression.
Article
Medicine, Research & Experimental
Peiwen Wang, Junwen Zhang, Qing Zhang, Fusheng Liu
Summary: The use of mesenchymal stem cells (MSCs) as vehicles of oncolytic adenovirus (oAd) is a promising strategy for enhancing the treatment efficacy of glioblastoma multiforme (GBM) by improving viral infiltration and protection from the host immune response.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Chemistry, Medicinal
Adriana G. Quiroz-Reyes, Paulina Delgado-Gonzalez, Jose F. Islas, Adolfo Soto-Dominguez, Carlos A. Gonzalez-Villarreal, Gerardo R. Padilla-Rivas, Elsa N. Garza-Trevino
Summary: This study evaluated the cytotoxic and proapoptotic activity of soluble TRAIL overexpressed by mesenchymal stem cells (MSC) in an oxaliplatin-resistant CRC cell line. The results demonstrated that oxaliplatin can enhance the apoptotic activity induced by soluble TRAIL.
Article
Nanoscience & Nanotechnology
Xin Liang, Hui Cai, Hao Sun, Na Li, Wenyue Zhao, Qiang Liu, Qiang Zeng, Yan Wang, Qing Gu
Summary: This study evaluated the efficacy of combining nanoscale exosomes derived from gingival mesenchymal stem cells (GMSCs) with radiotherapy (RT) in treating non-small cell lung carcinoma (NSCLC). The results showed that GMSCs-exo possessed a cup-shaped morphology with an average diameter of approximately 142 nm. The combination of GMSCs-exo and ionizing radiation (IR) demonstrated enhanced efficacy in inhibiting proliferation and promoting apoptosis of NSCLC cells, compared to IR treatment alone. In vivo experiments also confirmed the suppression of tumor growth upon administration of GMSCs-exo in combination with RT. Thus, this study highlights the potential clinical applications of MSC-derived exosomes in tumor radiotherapy.
ACS APPLIED NANO MATERIALS
(2023)
Article
Cell & Tissue Engineering
Zujia Wang, Hongting Chen, Peiyun Wang, Miaojin Zhou, Guangxu Li, Zhiqing Hu, Qian Hu, Junya Zhao, Xionghao Liu, Lingqian Wu, Desheng Liang
Summary: Mesenchymal stem cells (MSCs) have shown promise as a cellular vehicle for delivering anti-cancer factors to tumors. In this study, TRAIL was specifically integrated into the rDNA locus of human-induced pluripotent stem cells (iPSCs) using a non-viral vector and TALENickases. The genetically modified iPSCs were then differentiated into TRAIL-expressing MSCs, which demonstrated strong anti-tumor effects on various cancers both in vitro and in vivo.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Article
Immunology
Zhihui Zhang, Yulin Kuang, Kui Ma, Yan Li, Xiaoming Liu, Yuru Shi, Xu Wu
Summary: This study investigates the therapeutic effects of bone marrow mesenchymal stem cells (BMSCs) overexpressing GCLc on acute lung injury (ALI). The results show that overexpression of GCLc in BMSCs can reduce oxidative stress, improve cell survival, and inhibit apoptosis. The anti-apoptotic effects of GCLc are mediated through the PI3K/AKT/Foxo1 pathway. In an ALI mouse model, GCLc-overexpressing BMSCs improve lung inflammation and pulmonary edema.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Cell Biology
Wenjie Li, Yiquan Li, Yingli Cui, Shanzhi Li, Yilong Zhu, Chao Shang, Gaojie Song, Zirui Liu, Zhiru Xiu, Jianan Cong, Tingyu Li, Xiao Li, Lili Sun, Ningyi Jin
Summary: The study found that the recombinant adenovirus Ad-VT significantly inhibited the growth of MCF-7-CSC cells and induced apoptosis, demonstrating a strong inhibitory effect in mice and providing a new theoretical basis for the future treatment of breast cancer.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Genetics & Heredity
Beatrice Tolksdorf, Sina Zarif, Juergen Eberle, Ahmet Hazini, Babette Dieringer, Franziska Jonsson, Florian Kreppel, Jens Kurreck, Henry Fechner
Summary: Silencing of Mcl-1 can overcome TRAIL resistance in melanoma cells and enhance the cytotoxicity of TRAIL-expressing adenovirus. The increase of apoptosis induced by Mcl-1 silencing is the main mechanism for this effect, which occurs in both TRAIL-sensitive and TRAIL-resistant melanoma cells.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2021)
Article
Chemistry, Medicinal
Hussein Hamad, Birgitte Brinkmann Olsen
Summary: The study showed that CBD decreased the viability of lung cancer stem cells and adherent lung cancer cells, induced cell death, and reduced the self-renewal of cancer stem cells. This suggests that CBD may be effective against treatment-resistant cancer stem cells and warrants further investigation into its mechanisms.
Article
Biochemistry & Molecular Biology
Yeon-Jee Kahm, Rae-Kwon Kim
Summary: BIRC5, also known as survivin, is a protein that negatively regulates apoptosis by inhibiting caspase activation. Its overexpression enables cancer cells to survive and divide. BIRC5 is highly expressed in stem cells and may be involved in the regulation of cancer stem cells (CSCs). The study shows that BIRC5 is a key factor in CSCs and epithelial to mesenchymal transition (EMT) regulation, and it regulates the secretion of plasminogen activator inhibitor-1 (PAI-1), which is involved in signaling mechanisms regulating CSCs and EMT.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Anatomy & Morphology
Daria S. Chulpanova, Zarema E. Gilazieva, Elvira R. Akhmetzyanova, Sevindzh K. Kletukhina, Albert A. Rizvanov, Valeriya V. Solovyeva
Summary: The study aimed to produce vesicles from mesenchymal stem cells with simultaneous overexpression of TRAIL, PTEN, and IFN-beta 1, and found that these vesicles could activate immune cells and induce apoptosis in various types of carcinomas in vitro. Further studies on animal models in vivo are required to fully understand the immunomodulatory and antitumor properties of these vesicles.
Review
Cell & Tissue Engineering
Ameneh Jafari, Mostafa Rezaei-Tavirani, Behrouz Farhadihosseinabadi, Hakimeh Zali, Hassan Niknejad
Summary: Human amniotic fluid/membrane mesenchymal stem cells have conflicting roles in cancer treatment, with some studies demonstrating their anticancer effects while others suggesting their potential to promote cancer progression.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Anna-Christina Rambow, Insa Aschenbach, Sofie Hagelund, Doaa Tawfik, Jan-Paul Gundlach, Sebastian Weisse, Nicolai Maass, Anna Trauzold
Summary: Binding of TRAIL to its death domain-containing receptors TRAIL-R1 and TRAIL-R2 can induce cell death and/or pro-inflammatory signaling. The importance of TRAIL and TRAIL-R1/R2 in tumor immune surveillance and cancer biology has meanwhile been well documented. TRAIL also binds to TRAIL-R3 and TRAIL-R4, which have regulatory functions in apoptotic and non-apoptotic signaling pathways. Knockdown of TRAIL-R4 affects the activation of apoptotic and non-apoptotic pathways in cancer cells, showing opposing effects on cell death and clonogenic survival. TRAIL-R4 also regulates the expression of anti-apoptotic proteins and affects the activity of AKT, ERK, p38 and NF-kappa B. This study provides evidence for the important role of endogenous TRAIL-R4 in cancer cells and improves the understanding of the complex TRAIL/TRAIL-R system in humans.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemical Research Methods
Kathleen Pappritz, Oliver Klein, Fengquan Dong, Nazha Hamdani, Arpad Kovacs, Lisa O'Flynn, Steve Elliman, Timothy O'Brien, Carsten Tschope, Sophie Van Linthout
Summary: This study investigates the therapeutic effect of CD362(+)-selected MSC on early onset experimental diabetic cardiomyopathy using IMS. CD362(+) MSCs were able to improve titin intensity distribution and shift the isoform ratio towards the more compliant form, N2BA. In contrast, WT and CD362(-) MSCs improved titin phosphorylation and protein kinase G activity, leading to an improvement in diastolic performance.
PROTEOMICS CLINICAL APPLICATIONS
(2021)
Article
Oncology
Paul Loftus, Luke Watson, Laura M. Deedigan, Eva Camarillo-Retamosa, Roisin M. Dwyer, Lisa O'Flynn, Matthew Griffin, Timothy O'Brien, Michael Kerin, Stephen J. Elliman, Laura R. Barkley
Summary: The study revealed that syndecan-2 expressed on a specific stromal cell population within tumors plays a crucial role in regulating tumor growth and metastasis through modulation of TGF beta signaling. Inhibiting the migratory and immunosuppressive properties of tumor-associated stromal cells (TASCs) can effectively suppress tumor growth and spread.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Review
Multidisciplinary Sciences
Jingjing He, Desheng Kong, Zhifen Yang, Ruiyun Guo, Asiamah Ernest Amponsah, Baofeng Feng, Xiaolin Zhang, Wei Zhang, Aijing Liu, Jun Ma, Timothy O'Brien, Huixian Cui
Summary: This meta-analysis study found that MSCs therapy may be an effective and safe intervention for individuals with diabetes. Significant reductions in fasting plasma glucose levels and HbA1c were observed, while no significant increase in fasting C-peptide levels was found. There were no significant differences in adverse events between the MSCs treatment group and control group.
Article
Endocrinology & Metabolism
Matthias von Herrath, Stephen C. Bain, Bruce Bode, Jesper Ole Clausen, Ken Coppieters, Leylya Gaysina, Janusz Gumprecht, Troels Krarup Hansen, Chantal Mathieu, Cristobal Morales, Ofri Mosenzon, Stine Segel, George Tsoukas, Thomas R. Pieber
Summary: This study aimed to investigate the combination of anti-interleukin-21 antibody and liraglutide for treating type 1 diabetes, and found that this combination therapy could preserve beta-cell function with a better safety profile compared to traditional immunomodulation methods.
LANCET DIABETES & ENDOCRINOLOGY
(2021)
Article
Surgery
Sara-Azhari Mohamed, Navian Lee Viknaswaran, Jonathan Doran, Clara Sanz-Nogues, Khalid Ahmed, Linda Howard, Muhammad Tubassam, Timothy O'Brien, Stewart Redmond Walsh
Summary: The study found that the ERICVA risk score system has fair validity in patients with critical limb ischemia, but cannot be considered a reliable single predictor of one year amputation-free survival. The simplified score showed almost identical performance to the full score and can be used as a replacement.
ANNALS OF VASCULAR SURGERY
(2021)
Article
Cell & Tissue Engineering
Yicheng Ding, Aisling O'Brien, Berta Marco de la Cruz, Meimei Yang, Yin Lu, Xiaohong Qian, Guangming Yang, Veronica McInerney, Janusz Krawczyk, Sally A. Lynch, Linda Howard, Nicholas M. Allen, Timothy O'Brien, Louise Gallagher, Sanbing Shen
Summary: NRXN1 deletions are commonly found in ASD and other neurodevelopmental/neuropsychiatric disorders. Derivation of iPSCs from different diseases involving different deletion regions is essential for studying NRXN1's numerous splicing variants.
STEM CELL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Ning Ge, Min Liu, Xiaoran Zhu, Janusz Krawczyk, Veronica McInerney, Sanbing Shen, Timothy O'Brien, Terence Prendiville
Summary: Three human induced pluripotent stem cell (hiPSC) lines were generated from a healthy 7-year-old boy using non-integrational Sendai reprogramming method expressing specific genes. The stem cells were characterized through morphology, immunofluorescence staining, RT-qPCR, and showed differentiation potential to all three germ layers with lineage verification and normal molecular karyotyping.
STEM CELL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Yicheng Ding, Aisling O'Brien, Berta Marco de la Cruz, Meimei Yang, Jacqueline Fitzgerald, Guangming Yang, Weidong Li, Veronica McInerney, Janusz Krawczyk, Sally A. Lynch, Linda Howard, Nicholas M. Allen, Timothy O'Brien, Louise Gallagher, Sanbing Shen
Summary: NRXN1 gene encodes numerous splicing variants, with NRXN1 alpha playing a crucial role in neuronal excitation and inhibition, and its deletion being associated with ASD. Induced pluripotent stem cells (iPSCs) derived from ASD patients carrying NRXN1 alpha(+/-) provide an opportunity for further investigating the function of NRXN1 alpha in human neurons and ASD.
STEM CELL RESEARCH
(2021)
Article
Cardiac & Cardiovascular Systems
Rutao Wang, Patrick W. Serruys, Chao Gao, Hironori Hara, Kuniaki Takahashi, Masafumi Ono, Hideyuki Kawashima, Neil O'leary, David R. Holmes, Adam Witkowski, Nick Curzen, Francesco Burzotta, Stefan James, Robert-Jan Van Geuns, Arie Pieter Kappetein, Marie-Angele Morel, Stuart J. Head, Daniel J. F. M. Thuijs, Piroze M. Davierwala, Timothy O'Brien, Valentin Fuster, Scot Garg, Yoshinobu Onuma
Summary: This study compared the 10-year all-cause death rates in patients with three-vessel disease or left main coronary artery disease who underwent either PCI or CABG. The results showed no significant difference in all-cause death rates at 10 years between PCI and CABG, regardless of diabetic status. However, there might be a survival benefit with CABG in patients with insulin-treated diabetes.
EUROPEAN HEART JOURNAL
(2022)
Article
Cardiac & Cardiovascular Systems
Kathleen Pappritz, Fengquan Dong, Kapka Miteva, Arpad Kovacs, Muhammad El-Shafeey, Bahtiyar Kerim, Lisa O'Flynn, Stephen Joseph Elliman, Timothy O'Brien, Nazha Hamdani, Carsten Tschoepe, Sophie Van Linthout
Summary: The research findings indicate that in an experimental model of early-onset diabetic cardiomyopathy, the impact of CD362(+) MSC application on cardiac function is less pronounced compared to other MSC populations, but still shows some therapeutic effects.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Oncology
Andrea Mohr, Tianyuan Chu, Christopher T. Clarkson, Greg N. Brooke, Vladimir B. Teif, Ralf M. Zwacka
Summary: The study showed a close relationship between Fas-mediated MSC proliferation and tumor progression in the microenvironment of pancreatic cancer. Patient transcriptomic data indicated that high FasL expression is associated with high levels of MSC markers, IL6, and CCL2.
Editorial Material
Medicine, General & Internal
James O'Connell
Summary: The article discusses the importance of using insulin pumps and continuous glucose monitors in diabetes technology, especially for patients with impaired awareness of hypoglycemia.
NEW ENGLAND JOURNAL OF MEDICINE
(2021)
Article
Cell & Tissue Engineering
Min Liu, Ning Ge, Jianhua Zhang, Meimei Yang, Fan Yang, Janusz Krawczyk, Deirdre Ward, Veronica McInerney, Timothy O'Brien, Sanbing Shen, Terence Prendiville
Summary: Long QT syndrome type 2 (LQT2) is associated with mutations in the KCNH2 gene, leading to loss of Kv11.1 channel function and disruption of channel assembly and trafficking in cells. Patient-derived iPSCs provide a valuable tool for studying disease pathology and identifying potential therapeutic targets.
STEM CELL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Min Liu, Ning Ge, Yongfeng Han, Jamie Reilly, Meimei Yang, Fan Yang, Janusz Krawczyk, Veronica McInerney, Timothy O'Brien, Terence Prendiville, Sanbing Shen
Summary: Skin punch biopsy from a healthy 51-year-old Caucasian male was used to derive dermal fibroblasts, which were reprogrammed into hiPSC lines using non-integrative Sendai viruses expressing OCT4, SOX2, KLF4, and c-MYC. The resulting iPSC lines, capable of expressing pluripotent markers and differentiating into cells of endodermal, mesodermal, and ectodermal origin, can serve as controls for human disease modeling and drug screening when combined with genome-editing and 3D systems.
STEM CELL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Clara Sanz-Nogues, Michael Creane, Sean O. Hynes, Xizhe Chen, Christine Ayu Lagonda, Katarzyna Goljanek-Whysall, Timothy O'Brien
Summary: The aim of this study was to develop and validate a multiparametric scoring tool for assessing the severity of skeletal muscle ischaemia in a commonly used preclinical animal model. The study found a significant negative correlation between the level of muscle ischaemia damage and the calf muscle weight and skeletal muscle fibre diameter.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2023)