Article
Immunology
Omkar Indari, Annu Rani, Budhadev Baral, Suleyman Ergun, Kiran Bala, Srikanth Karnati, Hem Chandra Jha
Summary: Viruses exploit peroxisomal compartment for successful infection. This study investigates the modulation of peroxisomal proteins in EBV transformed cell lines and during acute EBV infection. The results reveal distinct regulation of peroxisomal proteins in EBV infected cells and highlight the importance of investigating the manipulation of the peroxisomal compartment in EBV pathologies.
MICROBIAL PATHOGENESIS
(2023)
Review
Biochemistry & Molecular Biology
Delara Akhter, Yuchan Zhang, Jianping Hu, Ronghui Pan
Summary: Protein ubiquitination plays a crucial role in regulating diverse cellular processes in eukaryotic organisms, including growth, development, and stress response. Despite its importance, the mechanisms underlying protein ubiquitination in peroxisomes, especially in plants, are poorly understood. This review provides a comprehensive summary of the current knowledge on the involvement of protein ubiquitination in peroxisome biology, focusing on plants.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Ning Zhang, Xufang Hu, Haolin Chen, Chunhui Deng, Nianrong Sun
Summary: A hydrophilic probe was utilized to enrich exosomes from cancer cells and distinguish them based on unique exosomal glycoproteins. The probe showed efficient performance in both processes, highlighting its potential for exosome research in cancer diagnosis and treatment.
CHEMICAL COMMUNICATIONS
(2021)
Article
Genetics & Heredity
Hans R. Waterham, Janet Koster, Merel S. Ebberink, Pavel Jesina, Jiri Zeman, Lenka Noskova, Stanislav Kmoch, Perrine Devic, David Cheillan, Ronald J. A. Wanders, Sacha Ferdinandusse
Summary: We report two unrelated patients with autosomal dominant Zellweger spectrum disorders (ZSDs). Biochemical and genetic studies revealed single heterozygous de novo variants in the PEX14 genes of both patients, leading to mRNA mis-splicing and expression of truncated PEX14 proteins. Functional studies demonstrated the dominant-negative effect of these truncated proteins on peroxisome functioning, which was restored by inhibition of pexophagy.
GENETICS IN MEDICINE
(2023)
Article
Multidisciplinary Sciences
Noa Dahan, Yury S. Bykov, Elizabeth A. Boydston, Amir Fadel, Zohar Gazi, Hodaya Hochberg-Laufer, James Martenson, Vlad Denic, Yaron Shav-Tal, Jonathan S. Weissman, Naama Aviram, Einat Zalckvar, Maya Schuldiner
Summary: This study discovered that translation of specific peroxisomal membrane proteins (PMPs) occurs on the surface of peroxisomes in yeast, similar to chloroplasts, mitochondria, and the endoplasmic reticulum. This localized translation process ensures the correct insertion of hydrophobic proteins into the peroxisomal membrane. Proper targeting of PMP transcripts to peroxisomes is crucial for cellular and peroxisomal function, highlighting the importance of localized translation in cellular physiology.
Editorial Material
Cell Biology
Seung Cho Lee, Robert A. Martienssen
Summary: Nuclear dicing bodies in plants are now understood as liquid-liquid phase-separated condensates driven by intrinsically disordered regions of the Dicing component SERRATE, which play a role in both phase separation and miRNA processing.
NATURE CELL BIOLOGY
(2021)
Article
Medical Laboratory Technology
Irene De Biase, Tatiana Yuzyuk, Wei Cui, Lauren M. Zuromski, Ann B. Moser, Nancy E. Braverman
Summary: Plasmalogens are glycerophospholipids with important roles in cellular processes, and their deficiency is associated with neurodegenerative diseases and peroxisome biogenesis disorders. A novel LC-MS/MS method was developed to quantify phosphoethanolamine plasmalogens in red blood cells, providing a specific and precise diagnostic tool. This method has the potential to enhance our understanding of disease pathogenesis and monitor therapy.
CLINICA CHIMICA ACTA
(2023)
Article
Cell Biology
Yuichi Yagita, Yuichi Abe, Yukio Fujiki
Summary: Previously, mammalian PEX16 was believed to be essential for peroxisomal membrane biogenesis and maintenance. However, recent studies found that pex16 mutants in other model organisms still have partially functional peroxisomes. It was also discovered that PEX16 accelerates the process of forming peroxisomal membranes in peroxisome-less cells, independently of its role in mediating peroxisomal targeting of PEX3.
JOURNAL OF CELL SCIENCE
(2022)
Article
Genetics & Heredity
Anuhya Anne, Sonal Saxena, Kommu Naga Mohan
Summary: Large DNA methylation changes were found in the gene promoters of cerebellum tissues of patients with autism spectrum disorder (ASD), including genes involved in peroxisome function. This supports the hypothesis that impaired peroxisome function/biogenesis is associated with ASD. Similar approaches could be used to identify rare epimutations in ASD and other complex disorders.
Article
Biochemistry & Molecular Biology
Mirco Schilff, Yelena Sargsyan, Julia Hofhuis, Sven Thoms
Summary: The study explores the impact of stop codon context on the treatment strategy for genetic premature termination codon diseases and finds that SCC plays a significant role in readthrough stimulation and drug concentration selection.
Review
Biochemistry & Molecular Biology
Annalisa Pecoraro, Martina Pagano, Giulia Russo, Annapina Russo
Summary: Cytosolic and mitochondrial ribosomes play crucial roles in protein synthesis and cellular functions. The biogenesis of ribosomes is tightly regulated and sensitive to various cellular stressors. Alterations in ribosome biogenesis are associated with tumorigenesis, and understanding the extra-ribosomal functions of ribosomal proteins is important for cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Hong-Bo Guo, Zhi-Fei Zhang, Jia-Qing Wang, Si-Yu Wang, Ji-Kang Yang, Xi-Yao Xing, Xiao-Jian Qi, Xiao-Dan Yu
Summary: This study provides insight into the molecular mechanism of autolysis in the fruiting bodies of Coprinus comatus by analyzing the transcriptome. Changes in metabolic pathways during the transition from mature stage to discolored stage were identified, providing a foundation for future studies on the autolysis of C. comatus fruiting bodies.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Stefan Gaussmann, Mohanraj Gopalswamy, Maike Eberhardt, Maren Reuter, Peijian Zou, Wolfgang Schliebs, Ralf Erdmann, Michael Sattler
Summary: In this study, the membrane interactions of PEX5 and PEX14 NTDs were characterized in vitro using membrane mimicking bicelles and nanodiscs, revealing weak interaction of PEX14 NTD with bicelles and multiple interaction sites of PEX5 NTD involving amphipathic alpha-helical regions. Interestingly, the interaction between PEX5 and PEX14 NTDs was found to be largely unaffected by the presence of the membrane, suggesting that docking of PEX5 to PEX14 at the membrane does not reduce the overall binding affinity between the two proteins in the assembly of the peroxisome translocon.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Zachary T. Neeb, Alexander J. Ritter, Lokendra V. Chauhan, Sol Katzman, W. Ian Lipkin, Nischay Mishra, Jeremy R. Sanford
Summary: The study found that SARS-CoV-2 infection causes global changes in host gene expression and differential expression of many host miRNAs. In addition, an expanded landscape of SARS-CoV-2-derived small viral RNAs (svRNAs) was discovered, which are predicted to interact with differentially expressed host mRNAs and miRNAs. These findings suggest that svRNAs may play a role in the propagation of SARS-CoV-2 and antagonizing them may have therapeutic value.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Zhenjing Ren, Kaijian Fan, Sihan Zhen, Jie Zhang, Yan Liu, Junjie Fu, Chunlai Qi, Qianhan Wei, Yao Du, Wurinile Tatar, Xiaofeng Zhang, Guoying Wang, Allan G. Rasmusson, Jianhua Wang, Yunjun Liu
Summary: The maize gene SMK11 encodes a mitochondria-localized tetratricopeptide repeat protein, which plays critical roles in the assembly and activity of mitochondrial complex IV, and is essential for seed development.
Article
Cell Biology
Mateusz Molon, Anita Panek, Eliza Molestak, Marek Skoneczny, Marek Tchorzewski, Maciej Wnuk
Article
Oncology
Magdalena Dabrowska, Marek Skoneczny, Lukasz Uram, Wojciech Rode
Article
Cell Biology
Blazej Kempinski, Anna Chelstowska, Jaroslaw Poznanski, Kamil Krol, Lukasz Rymer, Zuzanna Frydzinska, Wolfgang Girzalsky, Adrianna Skoneczna, Ralf Erdmann, Marek Skoneczny
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Genetics & Heredity
Joanna Klim, Urszula Zielenkiewicz, Anna Kurlandzka, Szymon Kaczanowski, Marek Skoneczny
Article
Multidisciplinary Sciences
Zhenzhong Yu, Jia Gao, Olumuyiwa Igbalajobi, Marek Skoneczny, Marzena Sienko, Agnieszka M. Maciejewska, Jerzy Brzywczy, Reinhard Fischer
Summary: The phytochrome-dependent light signaling pathway in fungi regulates the expression of genes related to stress responses and secondary metabolite production. The central regulator MetR plays a crucial role in controlling the expression of sulfur-assimilation genes and hundreds of other genes. Light and phytochrome also regulate the expression of carbon-sulfur hydrolases, providing insight into the interplay between light sensing and metabolic pathways.
Article
Biochemistry & Molecular Biology
Katarzyna Kosiorek, Anna Koryszewska-Baginska, Marek Skoneczny, Lidia Stasiak-Rozanska, Tamara Aleksandrzak-Piekarczyk
Summary: The L. lactis IL594 strain contains seven plasmids and is the parental strain of the plasmid-free L. lactis IL1403. Transcriptomic studies revealed that plasmidic genes could influence overall gene expression in industrially important L. lactis strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Magdalena Dabrowska, Agnieszka Kepczynska, Katarzyna Gozdzik, Malgorzata Calka-Kresa, Marek Skoneczny, Zbigniew Zielinski, Maria Doligalska, Ewa Sikora
Summary: The study found that muscle larva of the parasitic nematode Trichinella spp. displayed markers of cellular senescence, including high senescence-associated beta-galactosidase activity, lipid deposition, DNA damage, and cell cycle inhibition. Oxidative stress and upregulation of antioxidant defense enzymes were also observed in the larva. The findings suggest that cellular senescence is a state that allows the long-term existence of muscle larva and provides it with antioxidant capacity, making senotherapy a potentially effective treatment for trichinosis.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Editorial Material
Cell Biology
Marek Skoneczny, Tania Francisco, Ralf Erdmann, Christos Gatsogiannis
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Katarzyna Kosiorek, Anna Koryszewska-Baginska, Marek Skoneczny, Tamara Aleksandrzak-Piekarczyk
Summary: This study found significant differences in the performance of L. lactis IL1403 without plasmids compared to L. lactis IL594 with plasmids. The presence of pIL2, pIL4, and pIL5 plasmids had the most pronounced effects on the metabolism of various carbon sources and the tolerance to antimicrobial compounds and heavy metal ions. Comparative transcriptomics revealed significant variations in the expression levels of multiple chromosomal genes due to the presence of single plasmids and the activity of all plasmids, suggesting both direct and indirect effects of plasmids on the observed phenotypic changes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Justyna Antoniuk-Majchrzak, Tuguldur Enkhbaatar, Anna Dlugajczyk, Joanna Kaminska, Marek Skoneczny, Daniel J. Klionsky, Adrianna Skoneczna
Summary: DNA double-strand breaks are harmful to cells, especially when there is no error-free repair pathway available. In this case, error-prone recombination pathways are used to repair the damage, leading to decreased cell viability due to genome rearrangements. Rad51 recombinase is a major protein involved in recombinational repair of DNA damage, and its level is regulated through the ubiquitin-dependent proteolytic pathway. Rad51 can be modified by both ubiquitin and SUMO, and these modifications affect its ability to form and disassemble DNA repair foci, influencing cell cycle progression and cell viability in genotoxic stress conditions.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Article
Ecology
Joanna Klim, Urszula Zielenkiewicz, Marek Skoneczny, Adrianna Skoneczna, Anna Kurlandzka, Szymon Kaczanowski
Summary: Our experimental study found that genetic interactions and module structure have limited effects on evolutionary trajectory after gene deletion, and minimal impact on short-term compensatory evolution. However, likely compensatory evolution was observed in functionally related genes, even if they do not directly interact.
BMC ECOLOGY AND EVOLUTION
(2021)
Article
Cell Biology
Urszula Natkanska, Adrianna Skoneczna, Marek Skoneczny
CELL STRESS & CHAPERONES
(2018)