Article
Cell Biology
Grant A. King, Rahel Wettstein, Joseph M. Varberg, Keerthana Chetlapalli, Madison E. Walsh, Ludovic C. J. Gillet, Claudia Hernandez-Armenta, Pedro Beltrao, Ruedi Aebersold, Sue L. Jaspersen, Joao Matos, Elcin Unal
Summary: King and Wettstein et al. have discovered that nuclear pore complexes undergo two distinct remodeling events during budding yeast meiosis. This study provides insights into the organization of the nuclear basket and reveals a conserved aspect of NPC plasticity.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Rojapriyadharshini Gandhimathi, Dorothea Pinotsi, Mario Koehler, Jorg Mansfeld, Caroline Ashiono, Tatjana Kleele, Sumit Pawar, Ulrike Kutay
Summary: During mitotic entry, nuclear pore complexes (NPCs) disintegrate and so do the large nuclear and cytoplasmic rings formed by Y-complexes. Y-complex Nups dissociate slower than other Nups during in vitro NPC disassembly and remain associated with mitotic ER membranes. Super-resolution microscopy reveals two types of higher-order assemblies of mitotic Y-complexes: large clusters at kinetochores and small focal ER-associated structures, which refine current models of nuclear reassembly.
Article
Cell Biology
Margarida Dantas, Andreia Oliveira, Paulo Aguiar, Helder Maiato, Jorge G. Ferreira
Summary: Mitotic entry is regulated by the activity of cyclin B1-CDK1 complexes. This study demonstrates that actomyosin-dependent nuclear tension during prophase regulates the nuclear translocation of cyclin B1 and has implications for chromosome segregation efficiency.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Genetics & Heredity
Mariel A. Pfeifer, Chang Hyun Khang
Summary: In this study, the nuclear envelope arrangement in the rice blast fungus, Magnaporthe oryzae, was determined to involve two conserved components, Nup84 and Src1. Live-cell super-resolution structured illumination microscopy showed colocalization of Nup84-tdTomato and Src1-EGFP within the nuclear envelope during interphase, as well as Nup84-tdTomato's association with the dividing nucleus. Additionally, appressorium development in the fungus was found to require mitotic nuclear migration through the germ tube.
FUNGAL GENETICS AND BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kenta Tsuchiya, Hisato Hayashi, Momoko Nishina, Masako Okumura, Yoshikatsu Sato, Masato T. Kanemaki, Gohta Goshima, Tomomi Kiyomitsu
Summary: Spindle assembly in human cells is regulated independently by the Ran pathway without equivalently regulating all Ran-regulated SAFs. Ran's functions in mitotic spindle assembly are dissected from its interphase functions, with Ran-GTP and importin-beta coordinately promoting specific interactions to maintain proper spindle length during prometaphase.
Article
Biochemistry & Molecular Biology
Agata Gluszek-Kustusz, Benjamin Craske, Thibault Legal, Toni McHugh, Julie P. Welburn
Summary: During mitosis, the microtubule crosslinker protein PRC1 recruits motor proteins CENP-E and Kif4A to the central spindle to coordinate chromosome segregation and cytokinesis. This recruitment is mediated by a conserved hydrophobic motif and is spatially and temporally regulated by CENP-E. The interaction between PRC1 and microtubule motor proteins is essential for controlling chromosome partitioning, maintaining central spindle integrity, and ensuring cytokinesis during anaphase.
Article
Cell Biology
Jana Uhlirova, Lenka Sebestova, Karel Fiser, Tomas Sieger, Jindriska Fiserova, Pavel Hozak
Summary: The nucleoporin TPR plays a crucial role in the regulation of genes related to muscle formation and function and has been shown to affect the morphology of myoblasts and myotubes. However, more evidence is needed to fully understand how TPR specifically influences the expression of myogenic genes.
Article
Cell Biology
Jennifer R. Aleman, Terra M. Kuhn, Pau Pascual-Garcia, Janko Gospocic, Yemin Lan, Roberto Bonasio, Shawn C. Little, Maya Capelson
Summary: Dosage compensation in Drosophila melanogaster involves a 2-fold transcriptional upregulation of the male X chromosome, which relies on the X-chromosome-binding male-specific lethal complex. This study shows that the nuclear pore component Mtor plays a key role in regulating the correct transcription levels from the male X chromosome by affecting the function of the MSL complex, rather than through defects in mRNA export. Depletion of Mtor results in selective upregulation of X chromosome target genes beyond the required 2-fold, further highlighting its importance in dosage compensation.
Article
Cell Biology
Aymen al-Rawi, Edward Kaye, Svitlana Korolchuk, Jane A. Endicott, Tony Ly
Summary: Ordered protein phosphorylation by CDKs is a key mechanism for regulating the cell cycle. We show how CDK1 activity and non-catalytic CDK1 subunits contribute to the choice of substrate and site of phosphorylation using a fixed cell kinase assay and phosphoproteomics. Contrary to the model of CDK1 as an exclusively proline-directed kinase, Cyclin A and Cks1 enhance non-proline-directed phosphorylation, preferably on sites with a +3 lysine residue.
Article
Cell Biology
Dini Kurnia Ikliptikawati, Nozomi Hirai, Kei Makiyama, Hemragul Sabit, Masashi Kinoshita, Koki Matsumoto, Keesiang Lim, Makiko Meguro-Horike, Shin-ichi Horike, Masaharu Hazawa, Mitsutoshi Nakada, Richard W. Wong
Summary: This study reveals the important roles of nuclear pore complexes (NPCs) in glioblastoma (GBM). It identifies genomic amplification of NUP107 in GBM and demonstrates that NUP107 is overexpressed along with the critical E3 ligase MDM2. Depletion of NUP107 inhibits GBM cell growth through p53 protein stabilization.
Article
Biochemistry & Molecular Biology
Debanjan Bhattacharjee, Sreeram Kaveti, Nishant Jain
Summary: STAT3, an oncogene associated with poor prognosis, can be ubiquitinated by APC/C CDH1 during mitosis, resulting in long-lasting anti-tumor responses. Inhibiting phosphatases can decrease STAT3 ubiquitination. Targeting mitosis may be a potential therapeutic window for treating STAT3-activated cancers.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2023)
Article
Cell Biology
Stylianos Didaskalou, Christos Efstathiou, Sotirios Galtsidis, Ilona Kesisova, Aliaksandr Halavatyi, Tountzai Elmali, Avgi Tsolou, Andreas Girod, Maria Koffa
Summary: Accurate chromosome segregation requires proper formation of the mitotic spindle, and the localization of HURP protein in close proximity to the chromosomes is crucial for this process. In this study, we investigated the impact of microtubule flux and phosphorylation of HURP at Ser627 on its dynamics using photoactivation and FRAP experiments. Additionally, we identified the interactions of HURP with various proteins during mitosis through immunoprecipitation assays. Our findings show that phosphorylation of HURP at Ser627 regulates its interaction with these partners, and HURP participates in at least two distinct complexes during metaphase to ensure its proper localization and promote the bundling and stabilization of kinetochore fibers.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Rachel A. Berg, James B. Moseley
Summary: The protein kinase Cdr2 promotes mitotic entry by organizing cortical oligomeric nodes and inhibiting Wee1. Increasing Cdr2 expression leads to hyperphosphorylation of Wee1 and reduction in cell size. Cdr2 sequesters Wee1 in cytoplasmic clusters, away from Cdk1 in the nucleus, acting as a dosage-dependent regulator of cell size.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Oncology
Yi Xiao, Yuanhong Chen, Aimin Peng, Jixin Dong
Summary: CTDSPL2, phosphorylated by CDK1 in mitosis, plays a crucial role in regulating cell mitosis, proliferation, and motility in pancreatic cancer. Depletion of CTDSPL2 or using a phosphorylation-deficient mutant hindered tumor growth in xenograft models. Downstream regulators of CTDSPL2, p21 and p27, were identified, providing an alternative molecular target for pancreatic cancer treatment.
Review
Oncology
Ignacio Campillo-Marcos, Raul Garcia-Gonzalez, Elena Navarro-Carrasco, Pedro A. Lazo
Summary: VRK1 is a nuclear Ser-Thr chromatin kinase that is not mutated in cancer, but is overexpressed in many types of tumors and linked to poor prognosis. It plays crucial roles in regulating cell proliferation and DNA damage response pathways, having both pro-tumorigenic effects and protective anti-tumor roles depending on the cellular context.
Article
Chemistry, Multidisciplinary
Vedagopuram Sreekanth, Animesh Kar, Sandeep Kumar, Sanjay Pal, Poonam Yadav, Yamini Sharma, Varsha Komalla, Harsh Sharma, Radhey Shyam, Ravi Datta Sharma, Arnab Mukhopadhyay, Sagar Sengupta, Ujjaini Dasgupta, Avinash Bajaj
Summary: DTX-PC NMs, engineered sub-100 nm nanomicelles, show promising efficacy in animal studies without causing systemic toxicity. By increasing the expression of tumor suppressor genes, these nanomicelles have the potential to improve the quality of life in cancer patients.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Mansoor Hussain, Aftab Mohammed, Shabnam Saifi, Aamir Khan, Ekjot Kaur, Swati Priya, Himanshi Agarwal, Sagar Sengupta
Summary: Mutations in Pol gamma A gene lead to PEO, which can be negatively regulated by ubiquitylation by MITOL, causing impaired mitochondrial entry. Some PEO patients' Pol gamma A variants are heavily ubiquitylated, preventing mitochondrial entry.
Article
Immunology
Iqbal Azmi, Md Imam Faizan, Rohit Kumar, Siddharth Raj Yadav, Nisha Chaudhary, Deepak Kumar Singh, Ruchika Butola, Aryan Ganotra, Gopal Datt Joshi, Gagan Deep Jhingan, Jawed Iqbal, Mohan C. Joshi, Tanveer Ahmad
Summary: A major bottleneck in scaling-up COVID-19 testing is the need for sophisticated instruments and trained healthcare professionals, as well as constraints in the supply chain. CASSPIT introduces a new approach that allows direct use of saliva samples for detection without the extra RNA extraction step.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mehak Zahoor Khan, Biplab Singha, Mohammad Farhan Ali, Khushman Taunk, Srikanth Rapole, Samudrala Gourinath, Vinay Kumar Nandicoori
Summary: The study identified AosR as a crucial transcription factor for Mycobacterium tuberculosis survival in the host by mitigating oxidative and nitrosative stress, and promoting growth in the murine lungs and spleen. AosR interacts with SigH to upregulate CysM-dependent cysteine biosynthesis pathway, which is essential for detoxifying host-derived radicals and enhancing bacterial survival in the intracellular environment.
Article
Ophthalmology
Aparna Rao, Prity Sahay, Munmun Chakraborty, Birendra Kumar Prusty, Sandhya Srinivasan, Gagan Deep Jhingan, Pragyan Mishra Snr, Rahul Modak, Mrutyunjay Suar
Summary: This study found that autophagy-related proteins may play a predominant role in cell death mechanisms in severe glaucoma patients, showing a stronger presence compared to apoptosis. After oxidative stress, autophagy genes in HTM cells were upregulated between 48-72 hours, while apoptosis-related genes showed a downregulation trend at 72 hours.
CLINICAL OPHTHALMOLOGY
(2021)
Article
Microbiology
S. Salini, Sinchana G. Bhat, Saba Naz, Ramanathan Natesh, R. Ajay Kumar, Vinay Kumar Nandicoori, Krishna Kurthkoti
Summary: Applying antibiotics to susceptible bacterial cultures generates persistent bacteria that remain susceptible to antibiotics but can endure them. Recent reports have found that antibiotic persisters of mycobacteria experience oxidative stress and develop resistance. Our study reveals that mycobacterial persisters activate the SOS response and upregulate the error-prone DNA polymerase DnaE2, leading to mutagenesis and rapid evolution of antibiotic resistance.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Shubhi Pandey, Punita Kumari, Mithu Baidya, Ryoji Kise, Yubo Cao, Hemlata Dwivedi-Agnihotri, Ramanuj Banerjee, Xaria X. Li, Cedric S. Cui, John D. Lee, Kouki Kawakami, Jagannath Maharana, Ashutosh Ranjan, Madhu Chaturvedi, Gagan Deep Jhingan, Stephane A. Laporte, Trent M. Woodruff, Asuka Inoue, Arun K. Shukla
Summary: The study uncovers that non-canonical 7TMRs, D6R and C5aR2, efficiently couple to parrs with distinct engagement of GRKs and activation of non-canonical downstream signaling pathways.
Correction
Cell Biology
Kalpana Rajanala, Anshuk Sarkar, Gagan Deep Jhingan, Raina Priyadarshini, Manisha Jalan, Sagar Sengupta, Vinay Kumar Nandicoori
JOURNAL OF CELL SCIENCE
(2022)
Article
Biochemical Research Methods
Shashi Shekhar Singh, Mohammad Shahid Mansuri, Sarah Naiyer, Devinder Kaur, Mridula Agrahari, Sandhya Srinivasan, Gagan Deep Jhingan, Alok Bhattacharya, Sudha Bhattacharya
Summary: Using a combination of proteomics and transcriptomics, we investigated the adaptive response of Entamoeba histolytica during infection. We developed an advanced proteomics workflow that enabled the identification of a large number of proteins and integrated this data with transcriptomics to unravel regulation at the transcriptional and translational levels. We found differential abundance of proteins under stress conditions and observed inconsistencies between transcription and protein abundance, indicating the importance of post-transcriptional regulation.
Article
Biochemistry & Molecular Biology
Biplab Singha, Debashree Behera, Mehak Zahoor Khan, Nitesh Kumar Singh, Divya Tej Sowpati, Balasubramanian Gopal, Vinay Kumar Nandicoori
Summary: Through conditional gene replacement, it was found that the o-A gene in Mtb is essential for the survival of the bacteria, and its depletion leads to severe survival defects. RNA-seq analysis showed that o-A directly or indirectly regulates around 57% of the Mtb transcriptome, including 28% of essential genes. Overexpression of o-B, which has about 64% similarity to o-A, failed to compensate for the absence of o-A, suggesting minimal functional redundancy.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biology
Saba Naz, Kumar Paritosh, Priyadarshini Sanyal, Sidra Khan, Yogendra Singh, Umesh Varshney, Vinay Kumar Nandicoori, Digby F. Warner
Summary: Using genome-wide association analysis, we found a strong association between mutations in DNA repair genes and multidrug resistance in clinical strains of Mycobacterium tuberculosis (Mtb). Knockout and complemented strains in Mycobacterium smegmatis and Mtb were used to evaluate the impact of these mutations on drug resistance evolution. Results showed that variant mutations compromised the functions of MutY and UvrB, contributing to better survival under antibiotic/host stress conditions.
Article
Materials Science, Biomaterials
Indranil Mondal, Syeda Warisul Fatima, Swati Priya, Sagar Sengupta, Sunil Kumar Khare, Ritu Kulshreshtha
Summary: A novel nanostructure called TGNFs has been developed for efficient delivery of anti-miR-210 to glioblastoma cells, resulting in decreased proliferation and migration and increased apoptosis. This method shows potential as a delivery agent in miRNA inhibition therapy.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Microbiology
Basanti Malakar, Komal Chauhan, Priyadarshini Sanyal, Saba Naz, Haroon Kalam, R. P. Vivek-Ananth, Lakshya Veer Singh, Areejit Samal, Dhiraj Kumar, Vinay Kumar Nandicoori
Summary: This study discovers the regulation of secretion of the virulence factor CFP10 in Mycobacterium tuberculosis (Mtb) through protein phosphorylation. The protein-protein interaction network analysis reveals a complex relationship between phosphorylation and secretion. The dynamic phosphorylation status of CFP10 influences the secretion of virulence factors and impacts bacterial virulence.
Article
Multidisciplinary Sciences
Animesh Kar, Dolly Jain, Sandeep Kumar, Kajal Rajput, Sanjay Pal, Kajal Rana, Raunak Kar, Somesh K. Jha, Nihal Medatwal, Prabhu Srinivas Yavvari, Nishant Pandey, Devashish Mehta, Harsh Sharma, Debanjan Bhattacharya, Manas K. Pradhan, Ravi Datta Sharma, Aasheesh Srivastava, Usha Agrawal, Arnab Mukhopadhyay, Sagar Sengupta, Veena S. Patil, Avinash Bajaj, Ujjaini Dasgupta
Summary: This study presents a hydrogel-mediated localized delivery of a combination therapy of docetaxel and carboplatin for triple-negative breast cancer (TNBC), which effectively induces tumor regression and modifies the immunosuppressive tumor microenvironment. The therapy activates apoptotic cell death and immunogenic cell death, leading to the clearance of metastatic tumors.
Article
Biochemical Research Methods
Mansoor Hussain, Shabnam Saifi, Aftab Mohammed, Sagar Sengupta
Summary: The article outlines a method for detecting the ubiquitylation of PolyA by MITOL in cells and in vitro, involving the overexpression of Flag-PolyA and MITOL and the use of specific antibodies for detection.