Article
Multidisciplinary Sciences
Changhe Ji, Jakob Bader, Pradhipa Ramanathan, Luisa Hennlein, Felix Meissner, Sibylle Jablonka, Matthias Mann, Utz Fischer, Michael Sendtner, Michael Briese
Summary: The study reveals a functional association between the Smn complex involved in spliceosome biogenesis and the 7SK complex involved in transcriptional regulation, showing that the interaction is enhanced by changes in transcriptional activity, leading to modulation of spliceosome production.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Edward M. Courchaine, Andrew E. S. Barentine, Korinna Straube, Dong-Ryoung Lee, Joerg Bewersdorf, Karla M. Neugebauer
Summary: Biomolecular condensation plays a key role in cellular compartmentalization, with proteins like SMN promoting condensation through tudor domain-DMA interactions. The asymmetry or symmetry of DMA influences the organization of nuclear MLOs, as revealed by sub-diffraction microscopy.
Article
Cell Biology
Maximilian Schilling, Archana B. Prusty, Bjoern Boysen, Felix S. Oppermann, Yannick L. Riedel, Alma Husedzinovic, Homa Rasouli, Angelika Koenig, Pradhipa Ramanathan, Juergen Reymann, Holger Erfle, Henrik Daub, Utz Fischer, Oliver J. Gruss
Summary: The study found that mTOR and ribosomal protein S6 kinase beta-1 are key regulators of the SMN complex's ability to condense in Cajal bodies, and phosphorylation is an important regulatory mechanism for the complex's activity.
Article
Biochemistry & Molecular Biology
Alexandra Bergfort, Tarek Hilal, Benno Kuropka, Ibrahim Avsar Ilik, Gert Weber, Tugce Aktas, Christian Freund, Markus C. Wahl
Summary: This study reveals that the intrinsically disordered protein TSSC4 interacts with PRPF8 Jab1/MPN domain and SNRNP200 helicase to inhibit helicase activity, align the proteins, facilitate U5 submodule formation, and block premature interaction of SNRNP200 with other spliceosomal factors, thus promoting snRNP assembly.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Lu He, Jinke Yang, Yu Hao, Xing Yang, Xijuan Shi, Dajun Zhang, Dengshuai Zhao, Wenqian Yan, Xintian Bie, Lingling Chen, Guohui Chen, Siyue Zhao, Xiangtao Liu, Haixue Zheng, Keshan Zhang
Summary: DDX20 is an RNA-decapping enzyme that plays important roles in cellular transcription, post-transcriptional modifications, and the innate immune system. It is involved in processes such as translation initiation, RNA splicing, and microRNA maturation. DDX20 also affects signaling pathways such as NF-kappa B and p53, and can influence viral replication and innate immune responses.
Article
Thermodynamics
V. V. S. Sricharan, Srinivasan Chandrasekaran
Summary: A novel floating wave energy converter (BFWEC) with multiple 'bean shape' floats and a hydraulic power-take-off system (HPTO) has been proposed. Numerical analysis and simulations were conducted to study the maximum capture width and output effects of BFWEC in different configurations, as well as the impact of HPTO damping coefficient on the output.
Article
Microbiology
Laura White, Bilgi Erbay, G. Eric Blair
Summary: Cajal bodies can be reorganized into microfoci during adenovirus infection. The protein p80-coilin forms a complex with L4-22K protein, facilitating the nuclear export of Ad mRNA.
Article
Cell Biology
Katheryn E. Lett, Madelyn K. Logan, Douglas M. McLaurin, Michael D. Hebert
Summary: MicroRNAs (miRNAs) are small noncoding RNAs that control gene expression by translational inhibition and destabilization of their target mRNAs. The study reveals a novel regulatory interaction between the Microprocessor components and Coilin, which affects miRNA biogenesis and target gene expression.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Article
Biochemistry & Molecular Biology
Alba Puente-Bedia, Maria T. Berciano, Olga Tapia, Carmen Martinez-Cue, Miguel Lafarga, Noemi Rueda
Summary: Down syndrome is characterized by cognitive impairments, which may be due to defective neurogenesis and abnormal neuronal populations. In the TS mouse model of DS, the triplication of Hsa21 orthologous genes leads to alterations in the nuclear architecture of hippocampal granular cells, potentially contributing to cognitive dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Huan Niu, Meng Zhao, Jing Huang, Jing Wang, Yang Si, Shan Cheng, Wei Ding
Summary: CBs may serve as indicators of drug sensitivity or resistance in tumor cells, while UHMK1 maintains the structure and morphology of CBs by regulating splicing events.
CELL COMMUNICATION AND SIGNALING
(2022)
Review
Plant Sciences
Zhen Lei, Ling Wang, Eun Yu Kim, Jungnam Cho
Summary: Gene expression can be modulated by epigenetic mechanisms, including liquid-liquid phase separation (LLPS) in plants, which plays a key role in the chromatin and small RNA pathways. LLPS provides a mechanistic explanation for the self-assembly of subcellular structures, contributing to the efficiency and specificity of certain cellular reactions.
Article
Materials Science, Multidisciplinary
Binod K. Rai, Ganesh Pokharel, Hasitha Suriya Arachchige, Seung-Hwan Do, Qiang Zhang, Masaaki Matsuda, Matthias Frontzek, Gabriele Sala, V. Ovidiu Garlea, Andrew D. Christianson, Andrew F. May
Summary: This study investigates the anisotropic magnetism in NdCoGe3 and reveals the complex magnetic order derived from modulated magnetic moments through thermodynamic and neutron diffraction measurements. The material hosts multiple magnetic phases, and the magnetic structures exhibit incommensurate properties with a unique propagation vector.
Article
Chemistry, Multidisciplinary
Patricia S. Ferreira, Jose F. Malta, Nuno A. G. Bandeira, Alexander Allgaier, Joris van Slageren, Jose A. Paixao, Manuel Almeida, Laura C. J. Pereira, Pedro T. Gomes
Summary: A new homoleptic Co(ii) complex with two highly sterically congested 2-formiminopyrrolyl N,N'-chelating ligands is reported, showing slow relaxation of magnetization at zero static field. This compound has a large zero-field splitting parameter D of -42.6(4) cm(-1), resulting in a spin-reversal energy barrier U-eff of 85 cm(-1).
CHEMICAL COMMUNICATIONS
(2022)
Article
Genetics & Heredity
Ken Saida, Junya Tamaoki, Masayuki Sasaki, Muzhirah Haniffa, Eriko Koshimizu, Toru Sengoku, Hiroki Maeda, Masahiro Kikuchi, Haruna Yokoyama, Masamune Sakamoto, Kazuhiro Iwama, Futoshi Sekiguchi, Kohei Hamanaka, Atsushi Fujita, Takeshi Mizuguchi, Kazuhiro Ogata, Noriko Miyake, Satoko Miyatake, Makoto Kobayashi, Naomichi Matsumoto
Summary: Cerebellar ataxia is a genetically heterogeneous disorder caused by biallelic loss-of-function variants in the GEMIN5 gene, leading to neurodevelopmental delay, hypotonia, and cerebellar atrophy/hypoplasia. Zebrafish models further confirmed the pathogenicity of these variants.
Article
Materials Science, Multidisciplinary
E. Malchukova
Summary: This study investigates the effect of rare earth codoping on the emission ability of aluminoborosilicate glasses. The results show that the luminescence intensity and reduction efficiency of Sm ions exhibit nonlinear behavior with increasing rare earth concentration.
MATERIALS RESEARCH BULLETIN
(2022)
Article
Biochemistry & Molecular Biology
Antonio Galarreta, Pablo Valledor, Patricia Ubieto-Capella, Vanesa Lafarga, Eduardo Zarzuela, Javier Munoz, Marcos Malumbres, Emilio Lecona, Oscar Fernandez-Capetillo
Summary: Inhibitors of USP7 lead to widespread activation of CDK1 throughout the cell cycle, causing DNA damage and toxicity to mammalian cells. Additionally, USP7 interacts with the phosphatase PP2A, facilitating its active localization in the cytoplasm, and inhibition of USP7 or PP2A results in similar changes in the phosphoproteome, including increased phosphorylation of CDK1 targets.
Article
Medicine, Research & Experimental
Lucia Zhu, Diana Retana, Pedro Garcia-Gomez, Laura Alvaro-Espinosa, Neibla Priego, Mariam Masmudi-Martin, Natalia Yebra, Lauritz Miarka, Elena Hernandez-Encinas, Carmen Blanco-Aparicio, Sonia Martinez, Cecilia Sobrino, Nuria Ajenjo, Maria-Jesus Artiga, Eva Ortega-Paino, Raul Torres-Ruiz, Sandra Rodriguez-Perales, Riccardo Soffietti, Luca Bertero, Paola Cassoni, Tobias Weiss, Javier Munoz, Juan Manuel Sepulveda, Pedro Gonzalez-Leon, Luis Jimenez-Roldan, Luis Miguel Moreno, Olga Esteban, Angel Perez-Nunez, Aurelio Hernandez-Lain, Oscar Toldos, Yolanda Ruano, Lucia Alcazar, Guillermo Blasco, Jose Fernandez-Alen, Eduardo Caleiras, Miguel Lafarga, Diego Megias, Osvaldo Grana-Castro, Carolina Nor, Michael D. Taylor, Leonie S. Young, Damir Vareslija, Nicola Cosgrove, Fergus J. Couch, Lorena Cusso, Manuel Desco, Silvana Mouron, Miguel Quintela-Fandino, Michael Weller, Joaquin Pastor, Manuel Valiente
Summary: We report a medium-throughput drug-screening platform based on organotypic cultures that evaluates inhibitors against metastases growing in situ. Through this approach applied to brain metastasis, vulnerabilities were identified and a blood-brain barrier permeable HSP90 inhibitor was found to be effective against mouse and human brain metastases. In situ proteomic analysis revealed a novel molecular program in brain metastasis and potential biomarkers of poor prognosis and resistance mechanisms. This study validates METPlatform as a potent resource for metastasis research and personalized management of metastatic disease.
EMBO MOLECULAR MEDICINE
(2022)
Article
Oncology
Nazely Diban, Marian Mantecon-Oria, Maria T. Berciano, Alba Puente-Bedia, Maria J. Rivero, Ane Urtiaga, Miguel Lafarga, Olga Tapia
Summary: Graphene nanoplatelets immobilized in polyacrylonitrile film substrates inhibit migratory activity of breast cancer cells and induce epithelial-like differentiation, leading to changes in cell morphology and adhesion. The graphene-rich regions play a crucial role in these effects.
CANCER NANOTECHNOLOGY
(2022)
Review
Nutrition & Dietetics
Ana Palanca, Francisco Javier Ampudia-Blasco, Jose T. Real
Summary: Thyroid cancer is increasing in incidence and vitamin D may have a role in its prevention. However, the exact role of vitamin D in thyroid cancer development is controversial. Some studies suggest elevated serum vitamin D levels have a protective role, while others show no inverse relationship with the occurrence of thyroid cancer. Circulating vitamin D concentration is inversely correlated with disease aggressiveness and poor prognosis.
Article
Biochemistry & Molecular Biology
Raquel de la Hoz, Nazely Diban, Maria T. Berciano, Carlos San Emeterio, Ane Urtiaga, Miguel Lafarga, Jose C. Rodriguez-Rey, Olga Tapia
Summary: In this study, we compared the synthesis of polyethyleneimine (PEI) nanoparticles using different methods and found that coaxial electrospraying is a promising technique for the production of therapeutic nanoparticle-based carriers. The nanoparticles synthesized by coaxial electrospraying showed efficient RNA-based drug encapsulation, increased nanoparticle surface availability for functionalization, high transfection efficiency, and hyperactivation of clathrin-independent endocytic pathways as the main intracellular uptake mechanism.
Review
Biochemistry & Molecular Biology
Jose Ramos-Vivas, Olga Tapia, Maria Elexpuru-Zabaleta, Kilian Tutusaus Pifarre, Yasmany Armas Diaz, Maurizio Battino, Francesca Giampieri
Summary: Hafnia alvei is a bacterium that has attracted attention in the medical and veterinary field. It produces a wide variety of molecules, which has led to interest in its use as a probiotic, its impact on the microbiota, and its presence in consumer foods. This bacterium is capable of producing Acyl Homoserine Lactones (AHLs), a type of quorum-sensing signaling molecule, and has been found to promote certain enzymatic activities in fermented foods. It also produces molecules that modify the organoleptic properties of products, particularly cheeses. Additionally, some strains of H. alvei have been found to produce antibacterial compounds and other compounds of interest in clinical medicine and the food industry.
Review
Biochemistry & Molecular Biology
Alba Corman, Oleksandra Sirozh, Vanesa Lafarga, Oscar Fernandez-Capetillo
Summary: The nucleolus is where ribosome biogenesis takes place, which is one of the most resource-intensive processes in eukaryotic cells. It is highly responsive to growth signaling and nucleolar insults, collectively known as nucleolar stress. Nucleolar alterations are a prominent feature in various human diseases, including cancer and neurodegeneration, and are also associated with aging. There have been numerous efforts to develop compounds targeting different aspects of nucleolar activity. This article provides an overview of therapeutic opportunities and current therapies for targeting nucleoli in different pathologies.
TRENDS IN BIOCHEMICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Raquel Frances, Jorge Mata-Garrido, Roberto de la Fuente, Maria Carcelen, Miguel Lafarga, Maria Teresa Berciano, Raquel Garcia, Maria A. Hurle, Monica Tramullas
Summary: Neuropathic pain is a prevalent and severe chronic syndrome that is often difficult to treat, and its development and maintenance may involve epigenetic mechanisms. This study shows that inhibition of miR-30c-5p leads to global DNA hypermethylation in neurons of the dorsal root ganglia and spinal dorsal horn in rats with sciatic nerve injury. This mechanism is mediated by the upregulation of DNMT3a and DNMT3b, which are targeted by miR-30c-5p. Additionally, the study found that the inhibition of miR-30c-5p resulted in hypomethylation of the promoter region of TGF-beta 1, leading to long-term protection against neuropathic pain development. Overall, this research highlights the importance of miR-30c-5p in the epigenetic mechanisms underlying neuropathic pain and suggests it as a potential therapeutic target.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Alba Puente-Bedia, Maria T. Berciano, Carmen Martinez-Cue, Miguel Lafarga, Noemi Rueda
Summary: Hippocampal granule cells in Ts65Dn mice exhibit important oxidative stress-associated alterations, including DNA damage, abnormalities in the proteasome and lysosomal systems, and mitochondrial structural defects. These changes may contribute to cellular senescence, accelerated aging, neurodegenerative diseases, and early development of Alzheimer's pathology.
Article
Cardiac & Cardiovascular Systems
Jacob A. Ross, Nathaly Arcos-Villacis, Edmund Battey, Cornelis Boogerd, Constanza Avalos Orellana, Emilie Marhuenda, Pamela Swiatlowska, Didier Hodzic, Fabrice Prin, Tim Mohun, Norman Catibog, Olga Tapia, Larry Gerace, Thomas Iskratsch, Ajay M. Shah, Matthew J. Stroud
Summary: This study reveals that Lem2 plays a crucial role in cardiac development during embryogenesis and its deficiency leads to underdevelopment of the heart. However, partial depletion of Lem2 in adult hearts does not significantly affect cardiac function. These findings provide important insights into the mechanisms underlying cardiomyopathy caused by mutations in the Lem2 gene and cardio-laminopathies in general.
CARDIOVASCULAR RESEARCH
(2023)
Article
Cell Biology
Joshua S. Titlow, Maria Kiourlappou, Ana Palanca, Jeffrey Y. Lee, Dalia S. Gala, Darragh Ennis, Joyce J. S. Yu, Florence L. Young, David Miguel Susano Pinto, Sam Garforth, Helena S. Francis, Finn Strivens, Hugh Mulvey, Alex Dallman-Porter, Staci Thornton, Diana Arman, Marissa J. Millard, Aino I. Jarvelin, Mary Kay Thompson, Martin Sargent, Ilias Kounatidis, Richard M. Parton, Stephen Taylor, Ilan Davis
Summary: This study surveyed the intracellular localization of 200 mRNAs and encoded proteins in the Drosophila larval nervous system. It found that post-transcriptional regulation is common, with a significant number of transcripts localizing at tips of glia/neurons. There was discordance between mRNA distribution and protein expression in the majority of genes studied, suggesting the extensive nature of post-transcriptional regulation.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Immunology
Jorge RuizdelRio, Pedro Munoz, Patricia Carreira, David Maestro, Jose L. Pablos, Ana Palanca, Jesus Merino, Anna Serrano-Mollar, Ramon Merino, Esther Tamayo, Marcos Lopez-Hoyos, Federico Diaz-Gonzalez, Victor Martinez-Taboada, Ana V. Villar
Summary: This study investigated the expression and function of Hsp90 alpha in skin fibrosis. The results showed that Hsp90 alpha is overexpressed in fibrotic conditions and is associated with disease severity in SSc patients.
JOURNAL OF IMMUNOLOGY
(2022)